Anaerobic Oxidation of Ethane, Propane, and Butane by Marine Microbes: A Mini Review

The deep ocean and its sediments are a continuous source of non-methane short-chain alkanes (SCAs) including ethane, propane, and butane. Their high global warming potential, and contribution to local carbon and sulfur budgets has drawn significant scientific attention. Importantly, microbes can use gaseous alkanes and oxidize them to CO2, thus acting as effective biofilters. A relative decrease of these gases with a concomitant 13C enrichment of propane and n-butane in interstitial waters vs. the source suggests microbial anaerobic oxidation. The reported uncoupling of sulfate-reduction (SR) from anaerobic methane oxidation supports their microbial consumption. To date, strain BuS5 isolated from the sediments of Guaymas Basin, Gulf of California, is the only pure culture that can anaerobically degrade propane and n-butane. This organism belongs to a metabolically diverse cluster within the Deltaproteobacteria called Desulfosarcina/Desulfococcus. Other phylotypes involved in gaseous alkane degradation were identified based on stable-isotope labeling and fluorescence in-situ hybridization. A novel syntrophic association of the archaeal genus, Candidatus Syntrophoarchaeum, and a thermophilic SR bacterium, HotSeep-1 was recently discovered from the Guaymas basin, Gulf of California that can anaerobically oxidize n-butane. Strikingly, metagenomic data and the draft genomes of ca. Syntrophoarchaeum suggest that this organism uses a novel mechanism for n-butane oxidation, distinct from the well-established fumarate addition mechanism. These recent findings indicate that a lot remains to be understood about our understanding of anaerobic SCA degradation. This mini-review summarizes our current understanding of microbial anaerobic SCA degradation, and provides an outlook for future research

Role for polo-like kinase 4 in mediation of cytokinesis.

The mitotic protein polo-like kinase 4 (PLK4) plays a critical role in centrosome duplication for cell division. By using immunofluorescence, we confirm that PLK4 is localized to centrosomes. In addition, we find that phospho-PLK4 (pPLK4) is cleaved and distributed to kinetochores (metaphase and anaphase), spindle midzone/cleavage furrow (anaphase and telophase), and midbody (cytokinesis) during cell division in immortalized epithelial cells as well as breast, ovarian, and colorectal cancer cells. The distribution of pPLK4 midzone/cleavage furrow and midbody positions pPLK4 to play a functional role in cytokinesis. Indeed, we found that inhibition of PLK4 kinase activity with a small-molecule inhibitor, CFI-400945, prevents translocation to the spindle midzone/cleavage furrow and prevents cellular abscission, leading to the generation of cells with polyploidy, increased numbers of duplicated centrosomes, and vulnerability to anaphase or mitotic catastrophe. The regulatory role of PLK4 in cytokinesis makes it a potential target for therapeutic intervention in appropriately selected cancers

Draft Genome Sequences of Three Closely Related Isolates of the Purple Nonsulfur Bacterium Rhodovulum sulfidophilum

We report here the draft genome sequences of three isolates of Rhodovulum sulfidophilum from a single population that will serve as a model system for understanding genomic traits that underlie metabolic variation within closely related marine purple nonsulfur bacteria in natural microbial communities

Pulmonary capillary hemangiomatosis: a lesson learned

Pulmonary capillary hemangiomatosis (PCH) is a rare and controversial entity that is known to be a cause of pulmonary hypertension and is microscopically characterized by proliferation of dilated capillary-sized channels along and in the alveolar walls. Clinically, it is mostly seen in adults. Clinical features are characterized by nonspecific findings such as shortness of breath, cough, chest pain, and fatigue. It can be clinically indistinguishable from pre-capillary pulmonary arterial hypertension disorders such as primary pulmonary arterial hypertension (PAH) or chronic thromboembolic pulmonary hypertension. However, the diagnostic distinction, which usually requires a multidisciplinary approach, is crucial in order to avoid inappropriate treatment with vasodilator medications usually used for PAH treatment. Prognosis of PCH remains poor with lung transplant being the only definitive treatment. We report an autopsy case of pulmonary capillary hemangiomatosis unmasked at autopsy that was treated with a prostacyclin analog, usually contraindicated in such patients. We emphasize that this entity should always be on the differential diagnosis in a patient with pulmonary hypertension and requires great vigilance on the part of the clinician, radiologist and pathologist to make the diagnosis and guide appropriate management

Near-Infrared Imaging of Early-Type Galaxies III. The Near-Infrared Fundamental Plane

Near-infrared imaging data on 251 early-type galaxies in clusters and groups are used to construct the near-infrared Fundamental Plane (FP) r_eff ~ sigma_0^1.53 _eff^-0.79. The slope of the FP therefore departs from the virial expectation of r_eff ~ sigma_0^2 _eff^-1 at all optical and near-infrared wavelengths, which could be a result of the variation of M/L along the elliptical galaxy sequence, or a systematic breakdown of homology among the family of elliptical galaxies. The slope of the near-infrared FP excludes metallicity variations as the sole cause of the slope of the FP. Age effects, dynamical deviations from a homology, or any combination of these (with or without metallicity), however, are not excluded. The scatter of both the near-infrared and optical FP are nearly identical and substantially larger than the observational uncertainties, demonstrating small but significant intrinsic cosmological scatter for the FP at all wavelengths. The lack of a correlation of the residuals of the near-infrared FP and the residuals from the Mg_2-sigma relation indicates that the thickness of these relations cannot be ascribed only to age or metallicity effects. Due to this metallicity independence, the small scatter of the near-infrared FP excludes a model in which age and metallicity effects ``conspire'' to keep the optical FP thin. All of these results suggest that the possible physical origins of the FP relations are complicated due to combined effects of variations of stellar populations and structural parameters among elliptical galaxies.Comment: to appear in The Astronomical Journal; 35 pages, including 13 Postscript figures and 1 table; uses AAS LaTeX style file

Near-Infrared Imaging of Early-Type Galaxies IV. The Physical Origins of the Fundamental Plane Scaling Relations

The physical origins of the Fundamental Plane (FP) scaling relations are investigated for early-type galaxies observed at optical and near-infrared wavelengths. The slope for the FP is shown to increase systematically with wavelength from the U-band through the K-band. A distance-independent construction of the observables is described which provides an accurate measurement of the change in the FP slope between any pair of bandpasses. The variation of the FP slope with wavelength is strong evidence of systematic variations in stellar content along the elliptical galaxy sequence. The intercept of the diagnostic relationship between log(D_K/D_V) and log(sigma_0) shows no significant dependence on environment within the uncertainties of the Galactic extinction corrections, demonstrating the universality of the stellar populations contributions at the level of Delta(V-K)=0.03 mag to the zero-point of the global scaling relations. Several other constraints on the properties of early-type galaxies --- the slope of the Mg_2-sigma_0 relation, the effects of stellar populations gradients, and deviations of early-type galaxies from a dynamically homologous family --- are included to construct an empirical, self-consistent model which provides a complete picture of the underlying physical properties which are varying along the early-type galaxy sequence. This empirical approach demonstrates that there are significant systematic variations in both age and metallicity along the elliptical galaxy sequence, and that a small, but systematic, breaking of dynamical homology (or a similar, wavelength independent effect) is required. Predictions for the evolution of the slope of the FP with redshift are described. [abriged]Comment: to appear in The Astronomical Journal; 40 pages, including 10 Postscript figures and 3 tables; uses AAS LaTeX style file

Endoplasmic reticulum and lysosomal Ca2+ stores are remodelled in GBA1-linked Parkinson disease patient fibroblasts.

Mutations in β-glucocerebrosidase (encoded by GBA1) cause Gaucher disease (GD), a lysosomal storage disorder, and increase the risk of developing Parkinson disease (PD). The pathogenetic relationship between the two disorders is unclear. Here, we characterised Ca2+ release in fibroblasts from type I GD and PD patients together with age-matched, asymptomatic carriers, all with the common N370S mutation in β-glucocerebrosidase. We show that endoplasmic reticulum (ER) Ca2+ release was potentiated in GD and PD patient fibroblasts but not in cells from asymptomatic carriers. ER Ca2+ signalling was also potentiated in fibroblasts from aged healthy subjects relative to younger individuals but not further increased in aged PD patient cells. Chemical or molecular inhibition of β-glucocerebrosidase in fibroblasts and a neuronal cell line did not affect ER Ca2+ signalling suggesting defects are independent of enzymatic activity loss. Conversely, lysosomal Ca2+ store content was reduced in PD fibroblasts and associated with age-dependent alterations in lysosomal morphology. Accelerated remodelling of Ca2+ stores by pathogenic GBA1 mutations may therefore feature in PD

Elimination of the vesicular acetylcholine transporter in the forebrain causes hyperactivity and deficits in spatial memory and long-term potentiation

Basal forebrain cholinergic neurons, which innervate the hippocampus and cortex, have been implicated in many forms of cognitive function. Immunolesion-based methods in animal models have been widely used to study the role of acetylcholine (ACh) neurotransmission in these processes, with variable results. Cholinergic neurons have been shown to release both glutamate and ACh, making it difficult to deduce the specific contribution of each neurotransmitter on cognition when neurons are eliminated. Understanding the precise roles of ACh in learning and memory is critical because drugs that preserve ACh are used as treatment for cognitive deficits. It is therefore important to define which cholinergic-dependent behaviors could be improved pharmacologically. Here we investigate the contributions of forebrain ACh on hippocampal synaptic plasticity and cognitive behavior by selective elimination of the vesicular ACh transporter, which interferes with synaptic storage and release of ACh. We show that elimination of vesicular ACh transporter in the hippocampus results in deficits in long-term potentiation and causes selective deficits in spatial memory. Moreover, decreased cholinergic tone in the forebrain is linked to hyperactivity, without changes in anxiety or depression-related behavior. These data uncover the specific contribution of forebrain cholinergic tone for synaptic plasticity and behavior. Moreover, these experiments define specific cognitive functions that could be targeted by cholinergic replacement therapy