A profile of poisoning in pediatric population in rural hospital in Jammu

Background: Acute poisoning is one of the commonest Pediatric emergencies and an important cause of morbidity and mortality. This study is aimed at understanding the incidence, aetiology and manner of poisoning, outcome among children less than 18 years reporting to Pediatric department at sub district Hospital level in Jammu region.Methods: All the children between one month and eighteen years of age reporting to the Pediatric Emergency of SDH Akhnoor, Jammu over a period of one year as a case of acute poisoning were included in the study. The information obtained from subjects/parents/guardians and examinations of subjects was recorded on a pretested proforma and analyzed.Results: A total of 150 patients were admitted as a case of poisoning over a study period of one year. Male: Female ratio was 1.41:1. Total incidence rate was 2.94/100 0 population over a period of one year. Majority of the cases were accidental (94.67%) while suicidal attempt cases were 5.33%. Most the cases (49.33%) were due to accidental ingestion of household products followed by drug ingestion (21.33%), food poisoning (14%) and then agricultural/industrial products (8%). Maximum patients (86.6%) recovered while 2% referred to higher centre and rest 11.3% either absconded or lost to follow up.Conclusions: Acute poisoning in children is usually accidental with favourable outcome. Death can happen depending upon nature of poison and the stage at which the patient is brought to the hospital. Poisoning can be prevented by increasing public awareness, counselling and right guidance to the parents.

Influence of different transplanting date and weed management practices on yield and quality of basmati rice (Pusa Basmati-1509)

A field experiment was conducted during kharif 2014 at students’ farm of Chaudhary Charan Singh Haryana Agricultural University, college of agriculture, Kaul (Kaithal). The experiment consisted of four transplanting dates (June 15, July 5, July 25 and August 15) in main plots and six weed control treatments in sub plots treatments consisted of pre-emergence application of pretilachlor, oxadiargyl alone and sequential application of pre and post emergence herbicides viz., pretilachlor fb bispyribac, oxadiargyl fb bispyribac, weed free check and unweeded check. Based on research investigation it was observed that early transplanting dates (June 15 and July 5) produced taller plant, higher tillers/m2 and crop dry matter accumulation at all growth stages. Early transplanting produced significantly higher number of effective tillers/m2 (263) along with higher number of filled grain/ panicle (85) than delayed planting (July 25 and August 15). The 1000-grain weight was not affected by time of transplanting. The highest grain yield (4363 kg/ha) was recorded under June 15 transplanting which was statistically at par to the grain yield (4058 kg/ha) obtained under July 5 transplanting. Among the weed management practices tried, weed free check resulted in the highest values of plant height (104.2 cm), tillers/m2 (305), crop dry matter accumulation (964 g/ m2), effective tillers/m2 (271), grains length (8.5 cm), filled grains/panicle (86.3) as well as grain (4516 kg/ha) and straw yield (5506 kg/ha) which were however, comparable to pre-emergence application of oxadiargyl followed by bispyribac-sodium applied at 25 DAT and pretilachlor followed by bispribac-sodium at 25DAT

Green synthesis of acetohydroxamic acid by thermophilic amidase of Bacillus smithii IIIMB2907

Acetohydroxamic acid is a pharmaceutically active metal chelating agent which has various applications in the field of medicine. Current study focuses on the enzymatic synthesis of acetohydroxamic acid catalysed by thermophilic amidase from Bacillus smithii IIIMB2907. Bacterial cells were grown in 7 L fermenter for amidase production and effect of pH, temperature and substrate concentration for the biotransformation of acetamide to acetohydroxamic acid was studied. Batch reaction was also successfully optimized at bench scale with the recovery of ≈ 81% acetohydroxamic acid (purified)

Green synthesis of acetohydroxamic acid by thermophilic amidase of Bacillus smithii IIIMB2907

373-377Acetohydroxamic acid is a pharmaceutically active metal chelating agent which has various applications in the field of medicine. Current study focuses on the enzymatic synthesis of acetohydroxamic acid catalysed by thermophilic amidase from Bacillus smithii IIIMB2907. Bacterial cells were grown in 7 L fermenter for amidase production and effect of pH, temperature and substrate concentration for the biotransformation of acetamide to acetohydroxamic acid was studied. Batch reaction was also successfully optimized at bench scale with the recovery of ≈ 81% acetohydroxamic acid (purified)

Microtopography of the eggshell of Menacanthus eurysternus (Phthiraptera: Amblycera)

The egg laying sites, pattern and the egg morphology (SEM) of an amblyceran louse parasitizing Bank Myna (Acridotheres ginginianus) have been recorded. Unlike most of the species of the genus, Menacanthus studied so far, the eggshell of M. eurysternus lacks the apophyses (bristle like outgrowths arising from anterior portion of the eggshell). However, the opercular disc of M. eurysternus bears a polar thread and the micropyles are set along the opercular rim

An Experimental Models (In-Vivo and In-Vitro) Used for the Study of Antidiabetic agents

Diabetes is divided into two main types, type 1 (insulin-dependent diabetes Mellitus) and type 2 (non-insulin dependent diabetes). About 90% worldwide diabetics have type 2 diabetes. They are different in vivo and in vitro methods for screening new diabetes drugs. Mainly in life models uses chemicals like streptozotocin, alloxan, etc. to induce diabetes in vitro methods directly demonstrate its effect on cells responsible for induction of diabetes in humans. In vitro techniques provide more accurate information and possible Mechanisms related to diabetes. Now the latest techniques of the day such as the induction of diabetes by viruses that have also been introduced proves to be a good tool in the evaluation of diabetes drugs. This review can prove it be a good tool for researchers who want to investigate the diabetes it provides a comprehensive resource on the diabetes model under one roof

Transcriptome and metabolite analyses in Azadirachta indica: identification of genes involved in biosynthesis of bioactive triterpenoids

Bhambhani S, Lakhwani D, Gupta P, et al. Transcriptome and metabolite analyses in Azadirachta indica: identification of genes involved in biosynthesis of bioactive triterpenoids. SCIENTIFIC REPORTS. 2017;7(1): 5043.Azadirachta indica A. Juss, commonly known as Neem, is the reservoir of triterpenoids of economic importance. Metabolite analysis of different developmental stages of leaf and fruit suggests tissue-specific accumulation of the major triterpenoids in this important tree. Though biosynthesis of these complex molecules requires substrate flux from the isoprenoid pathway, enzymes involved in late biosynthetic steps remain uncharacterized. We established and analyzed transcriptome datasets from leaf and fruit and identified members of gene families involved in intermediate steps of terpenoid backbone biosynthesis and those related to secondary transformation leading to the tissue-specific triterpenoid biosynthesis. Expression analysis suggests differential expression of number of genes between leaf and fruit and probable participation in the biosynthesis of fruit-specific triterpenoids. Genome-wide analysis also identified members of gene families putatively involved in secondary modifications in late biosynthetic steps leading to the synthesis of highly oxygenated triterpenoids. Expression and molecular docking analyses suggest involvement of specific members of CYP450 family in secondary modifications for the biosynthesis of bioactive triterpenoids. This study generated rich genomic resource and identified genes involved in biosynthesis of important molecules, which will aid in the advancement of tools for functional genomics and elucidation of the biosynthesis of triterpenoid from this important tree