Quantifying Oxygen Content in Tungsten by Ion Beam Analysis

Treballs Finals de Grau de Física, Facultat de Física, Universitat de Barcelona, Curs: 2016, Tutors: Thomas Schwarz-Selinger i Antoni García-SantiagoThe quantitative analysis of 16O in near-surface regions of samples can be performed with 16O(3He,p)18F reaction. The cross section for this nuclear reaction in SiO2 samples has been determined at a reaction angle 135 for 3He energies in the laboratory frame between 1800 keV and 6100 keV. A relation between the number of protons collected as a result of the nuclear reaction and the amount of oxygen in tungsten samples has been determined

Procesos de apropiación adolescente del espacio público : otra cara de la renovación urbanística en Barcelona

Este artículo presenta el papel de la edad como eje de análisis en el marco de la geografía social y cultural. El trabajo de campo incluye grupos de discusión, entrevistas individuales e itinerarios participativos por el barrio, para identificar cuáles son los lugares frecuentados, los lugares preferidos y los lugares evitados por este grupo de población. Nos interesa conocer como desarrollan su vida en el barrio, como ocupan su tiempo cotidiano y como utilizan los espacios públicos. Analizar la vida en el barrio es esencial para entender las diferentes necesidades e intereses de chicos y chicas en relación a sus experiencias y percepciones de los espacios vividosThis article presents the role of age as an important axis of analysis in the framework of social and cultural geography. The fieldwork includes focus groups, interviews and walking tours around the neighborhood, to identify the more used places, the preferred places and the places young people don't like. We want to know how they build their lives in the neighborhood, how they spend their time and how they use public spaces. Analyzing the neighborhood life is essential to understand the different needs and interests of boys and girls in relation to their experiences and perceptions of lived space

Los lugares de la amistad y la vida cotidiana de chicas y chicos adolescentes en un barrio de Barcelona

Este artículo presenta los rasgos que caracterizan la amistad adolescente entre mujeres y varones, sus principales redes y los espacios donde se relacionan y se expresan. El estudio de caso se localiza en el barrio del Besòs de Barcelona (España). En un contexto de fuertes contrastes sociales y urbanísticos, los y las adolescentes construyen sus identidades, donde viven la experiencia de la amistad como red de apoyo mutuo y de solidaridad. A partir de grupos de discusión y entrevistas se ha obtenido información sobre los tiempos y los lugares de la amistad. Concretamente, se identifican cuáles son los lugares del barrio que frecuentan, los lugares preferidos para pasar su tiempo libre, así como los lugares que evitan frecuentar. La amistad emerge como una categoría que se cruza con las de etnicidad, género y clase, y que, a través de las prácticas sociales y espaciales cruza fronteras reales e imaginariasThis paper examines which are the features of adolescent boys and girls friendships, their main networks and spaces where they interact and express themselves. The case study is based on a field work in Besòs district, in the city of Barcelona (Spain). In this context, with relative social inequalities, adolescents are struggling to build up their identities and living, at the same time, the experience of friendship as a network of mutual support and solidarity. Data from focus groups and interviews gave us information about the times and places where groups of teenager friends meet and interact. Specifically, we've identified the places frequented by them, their favorite places, as well as the places they avoid. From their narratives, friendship emerges as a kind of place, as a category that crosses with those of ethnicity, gender and class, when and where teenagers' spatial and social practices of friendship shape real and imagined border

Lectures de l'impacte de la crisi econòmica en al vida quotidiana dels infants a Catalunya

La crisi econòmica i financera afecta intensament la vida quotidiana de les famílies i la infància a Catalunya. Utilitzant una metodologia de tipus qualitatiu, s'estudia el tractament d'aquest tema en els principals informes especialitzats i la premsa. Aquesta aproximació documental es complementa amb entrevistes a persones expertes de l'àmbit educatiu sobre la seva percepció del fenomen. S'observa que cada cop més famílies viuen situació de pobresa i vulnerabilitat. Les escoles i altres espais educatius viuen canvis significatius i s'hi adapten. Les famílies incrementen l'acompanyament dels infants a les escoles i redistribueixen tasques de la llar i de cura de la família entre el seus membres. Les entitats d'atenció social veuen disminuir els recursos mentre les demandes augmenten i es diversifiquen. Aquests efectes de la crisi no són iguals per a tota la població, ni a tot arreu. Es pretén analitzar aquestes diferències socials i espacials incorporant-hi una perspectiva de gènere.Reading on the economic crisis impact on children’s daily life in Catalonia The financial and economic crisis in countries of Southern Europe affects the daily lives of children and young people in Catalonia. Using a qualitative methodology, this paper focus on how this issue is treated by specialized reports and the press. This first approach is complemented with interviews to experts, from the field of education, on their perception of the phenomenon. More and more families live situations of poverty and vulnerability. Schools and after school activities experiment significant changes and try to adapt to them. Moreover, parents are taking children to school more often and housework and family care are readjusted among family members. Accordingly, in a context of fewer resources for social care organizations, the demand increases and become more diversified. The impact of the crisis on children’s daily lives varies in different locations. This article aims to analyse the social and spatial differences taking into account the gender perspective.Lecturas del impacto de la crisis económica en la vida cotidiana de la infancia en Cataluña La crisis económica y financiera afecta intensamente la vida cotidiana de las familias y la infancia de Cataluña. Utilizando una metodología de tipo cualitativo, se estudia cómo se trata este tema en los principales informes especializados y la prensa. Esta aproximación documental se complementa con entrevistas a personas expertas del ámbito educativo sobre su percepción del fenómeno. Se observan cada vez más familias que viven situaciones de pobreza y vulnerabilidad. Las escuelas y otros espacios educativos viven cambios significativos y se adaptan a ellos. Las familias incrementan su tarea de acompañamiento en las escuelas y redistribuyen las tareas del hogar y del cuidado de la familia. Las entidades de atención social ven disminuir los recursos mientras las demandas aumentan y se diversifican. Estos efectos de la crisis no son iguales para toda la población, ni en todos los lugares. Se pretende analizar estas diferencias sociales y espaciales incorporando una perspectiva de género

Differential pattern of glycogen accumulation after protein phosphatase 1 glycogen-targeting subunit PPP1R6 overexpression, compared to PPP1R3C and PPP1R3A, in skeletal muscle cells

Background PPP1R6 is a protein phosphatase 1 glycogen-targeting subunit (PP1-GTS) abundant in skeletal muscle with an undefined metabolic control role. Here PPP1R6 effects on myotube glycogen metabolism, particle size and subcellular distribution are examined and compared with PPP1R3C/PTG and PPP1R3A/GM. Results PPP1R6 overexpression activates glycogen synthase (GS), reduces its phosphorylation at Ser-641/0 and increases the extracted and cytochemically-stained glycogen content, less than PTG but more than GM. PPP1R6 does not change glycogen phosphorylase activity. All tested PP1-GTS-cells have more glycogen particles than controls as found by electron microscopy of myotube sections. Glycogen particle size is distributed for all cell-types in a continuous range, but PPP1R6 forms smaller particles (mean diameter 14.4 nm) than PTG (36.9 nm) and GM (28.3 nm) or those in control cells (29.2 nm). Both PPP1R6- and GM-derived glycogen particles are in cytosol associated with cellular structures; PTG-derived glycogen is found in membrane- and organelle-devoid cytosolic glycogen-rich areas; and glycogen particles are dispersed in the cytosol in control cells. A tagged PPP1R6 protein at the C-terminus with EGFP shows a diffuse cytosol pattern in glucose-replete and -depleted cells and a punctuate pattern surrounding the nucleus in glucose-depleted cells, which colocates with RFP tagged with the Golgi targeting domain of β-1,4-galactosyltransferase, according to a computational prediction for PPP1R6 Golgi location. Conclusions PPP1R6 exerts a powerful glycogenic effect in cultured muscle cells, more than GM and less than PTG. PPP1R6 protein translocates from a Golgi to cytosolic location in response to glucose. The molecular size and subcellular location of myotube glycogen particles is determined by the PPP1R6, PTG and GM scaffolding

Automatic system for electroporation of adherent cells growing in standard multi-well plates

In this study an automatic system is presented to perform electroporation, also known as electropermeabilization, on adherent cells. It is an intention of this system to apply electric field pulses directly to cells growing in standard multiwell plates as a step forward to include this technique in standard laboratory protocols. An interdigitated microelectrode assembly constructed with Printed Circuit Board (PCB) is placed closely above the cell monolayer, and in order to avoid direct contact with cells, small micro-separators were included in the structure. Additionally, distribution of current density was modified by filling the gap between adjacent electrodes with a non conductive material as predicted by electric field simulations. This modification helps to concentrate the electric field intensity in the region where cells are present. The device was tested using C2C12 cell line growing adhered in 24 multiwell plates and fluorescent labeled dextran FD20S as the molecule to be delivered. Successful transfection was observed with minimal invasiveness of the operation reducing the stress caused to cells.Postprint (published version

Morfologia i formació del complexe sinaptinèmic a espermatòcits i oòcits de Mus musculus

A method for the sequential study of synaptonemal complexes by light and electron microscopy has been used to characterize the synaptic process in mouse (Mus musculus) spermatocytes and oocytes. Pre- and post-synaptic figures can be easily identified