Regional differences in porcine adipocytes isolated from skeletal muscle and adipose tissues as identified by a proteomic approach.

International audienceThe content and distribution of body lipids are of special interest for production efficiency and meat quality in the farm animal industry. Triglycerides represent the most variable fraction of tissue lipids, and are mainly stored in adipocytes. Although several studies have reported regional differences in the expression of genes and their products in adipocytes from various species, the characteristics of i.m. adipocytes remain poorly described. To evaluate adipocyte features according to muscle and other fat locations, adipocyte proteins were isolated from trapezius skeletal muscle, and intermuscular, s.c., or perirenal adipose tissues from 6 female pigs (80 d of age). Protein extracts were labeled and analyzed by 2-dimensional, fluorescent, differential gel electrophoresis. The comparisons revealed that 149 spots were always differentially expressed (P < 0.05, ratio exceeding |2|-fold difference) between i.m. adipocytes and the fat cells derived from the 3 other adipose locations. The proteins that were downregulated in i.m. fat cells belonged to various metabolic pathways, such as lipogenesis (cytosolic malate dehydrogenase and isocitrate dehydrogenase, P < 0.01), glycolysis (enolases and aldolase,

Caractéristiques métaboliques des adipocytes selon leur origine tissulaire chez le porc : mise en place d'une étude du transcriptome

National audienc

Propriétés histologiques et métaboliques du muscle squelettique chez le mini-porc obèse et insulino-résistant

National audienc

Propriétés histologiques et métaboliques du muscle squelettique chez le mini-porc obèse et insulino-résistant

National audienc

Appraising the shelf life of farmed cobia, Rachycentron canadum, by application of a quality index method.

Freshness is the main concern of seafood quality, and the principal method to evaluate seafood freshness is sensory evaluation. The aim of this work was to study the quality changes of cobia, Rachycentron canadum, under ice storage through sensory and physical analysis as well as bacterial counting of specific spoilage organisms (SSOs). In particular, the utilization of a quality index method (QIM) scheme was proposed. Samples stored for 0-30 d were analyzed with the QIM.Autoria: PEREIRA, A. [i.e. M.] L.; Alitiene [i.e. Moura] Lemos Pereira. Version of record online: 21 jul. 2016