Thyroid hormone receptor binding to DNA and T(3)-dependent transcriptional activation are inhibited by uremic toxins

BACKGROUND: There is a substantial clinical overlap between chronic renal failure (CRF) and hypothyroidism, suggesting the presence of hypothyroidism in uremic patients. Although CRF patients have low T(3 )and T(4 )levels with normal thyroid-stimulating hormone (TSH), they show a higher prevalence of goiter and evidence for blunted tissue responsiveness to T(3 )action. However, there are no studies examining whether thyroid hormone receptors (TRs) play a role in thyroid hormone dysfunction in CRF patients. To evaluate the effects of an uremic environment on TR function, we investigated the effect of uremic plasma on TRβ1 binding to DNA as heterodimers with the retinoid X receptor alpha (RXRα) and on T(3)-dependent transcriptional activity. RESULTS: We demonstrated that uremic plasma collected prior to hemodialysis (Pre-HD) significantly reduced TRβ1-RXRα binding to DNA. Such inhibition was also observed with a vitamin D receptor (VDR) but not with a peroxisome proliferator-activated receptor gamma (PPARγ). A cell-based assay confirmed this effect where uremic pre-HD ultrafiltrate inhibited the transcriptional activation induced by T(3 )in U937 cells. In both cases, the inhibitory effects were reversed when the uremic plasma and the uremic ultrafiltrate were collected and used after hemodialysis (Post-HD). CONCLUSION: These results suggest that dialyzable toxins in uremic plasma selectively block the binding of TRβ1-RXRα to DNA and impair T(3 )transcriptional activity. These findings may explain some features of hypothyroidism and thyroid hormone resistance observed in CRF patients

A novel route to optimize placement equipment kinematics by coupling capacitive accelerometers

Machine end-effector kinematic analysis is critical to optimizing transporting components where inertial forces are the main loads. While displacements may be measured with relatively high accuracy in transportation equipment motors, the inertial forces in the transported components are seldom optimized. This is especially relevant in electronic component placement systems, where the components have a wide range of configurations (i.e., geometry and mass) and the deployment dimensional/geometric tolerances are remarkably good. The optimization of these systems requires the monitoring of the real position of the accelerometers relative to the measurement point of interest with sufficient accuracy that allows the assembly position to be predicted instantaneously. This study shows a novel method to calibrate this equipment using triaxial accelerometers on a surface mount machine to measure the end-effector accelerations and velocities in its planar motion. The dynamic equations of the system and the method for integration are presented to address the uncertainty on the exact position of the accelerometer sensors relative to the measuring point of interest exist and allow the position correction to optimize response and accuracy.European Structural and Investment Funds in the FEDER component through the Operational Competitiveness and Internationalization Programme (COMPETE 2020) [Project no. 39479; Funding Reference: POCI-01-0247-FEDER-39479]

Community-acquired invasive liver abscess syndrome caused by a K1 serotype Klebsiella pneumoniae isolate in Brazil: a case report of hypervirulent ST23

Universidade Federal de São Paulo (UNIFESP) Departamento de MedicinaUniversidade Federal de São Paulo (UNIFESP) Departamento de Medicina Divisão de Doenças InfecciosasUNIFESP, Depto. de MedicinaUNIFESP, Depto. de Medicina Divisão de Doenças InfecciosasSciEL

The physics as structure of technical knowledge from the perspective of students.

Discutir as interfaces entre a educa??o cient?fica e a educa??o profissional e tecnol?gica envolve diversos fatores que comp?em as condi??es concretas das atividades de ensino e aprendizagem. Com o intuito de ampliar o debate sobre a interdisciplinaridade entre as componentes curriculares conhecidas como proped?uticas e t?cnicas, este trabalho foca a percep??o do estudante. Os discentes s?o sujeitos essenciais na composi??o institucional e, em ?ltima an?lise, raz?o para exist?ncia das institui??es de ensino. Dentre os diversos aspectos que comp?em as intera??es entre a educa??o cient?fica e a educa??o profissional, o objetivo do artigo ? apresentar e discutir resultados de pesquisa sobre a percep??o dos estudantes acerca das rela??es dos conte?dos da componente curricular F?sica na composi??o dos cursos profissionalizantes. Para tanto, foi criado, validado e aplicado um question?rio para coleta de dados e posterior an?lise quantitativa; o m?todo adotado foi a estat?stica descritiva. A amostra ? constitu?da por 249 estudantes do segundo e terceiro anos de cursos t?cnicos integrados ao Ensino M?dio do IFMG, Campus Ouro Preto (IFMG-OP).Discussing the interfaces between scientific education and professional and technological education involves several factors that compose the concrete conditions of teaching and learning activities. With the aim of expand the debate about the interdisciplinarity between the curricular components known as propedeutics and techniques, this paper focuses on the student's perception. The students are essential subjects in the institutional composition and, ultimately, reason for the existence of the educational institutions. Among the several aspects that compose the interactions between scientific education and professional education, the objective of this paper is to present and discuss results of students' perceptions about the content relations of the Physics curricular component in the composition of professional courses. For that, a questionnaire was created, validated and applied for data collection and posterior quantitative analysis; the method adopted was descriptive statistics. The sample consists of 249 students of the second and third years of technical courses integrated to the high school of the IFMG, Campus Ouro Preto (IFMG-OP)

double-blind crossover study

Background: to evaluate the effects of one week of supplementation with curcumin combined with piperine on physical performance, immune system cell counts, muscle damage, and plasma levels of inflammatory markers after a treadmill running training session. Methods: This study is a double-blind, crossover-balanced clinical trial with a three-week intervention. Sixteen male runners with a mean age of 36 ± 9 years and VO2 max of 60.6 ± 9.03 mL.kg −1 min −1 were recruited and randomly divided into 2 groups: the first group (CPG) was supplemented daily for 7 days with 500 mg of curcumin + 20 mg piperine, and the second group (PG) was supplemented with 540 mg of cellulose. After the 7th day of supplementation, the volunteers participated in the experimental running protocol, where blood samples were collected before, after, and one hour after exercise for analysis of the number of leukocytes, creatine kinase, and cytokine concentration (IL-2, TNF-α, IFN, IL-6, and IL-10) using flow cytometry. This process was repeated, reversing the supplementation offered to the groups. Results: curcumin and piperine supplementation could not change the physical performance, immune cell counts, and muscle damage; however, the aerobic fatiguing exercise protocol inhibited the elevation of the plasmatic levels of some cytokines. The running exercise protocol could elevate the circulating levels of IL-2 (from 49.7 to 59.3 pg/mL), TNF-α (from 48.5 to 51.5 pg/mL), INF (from 128.8 to 165.0 pg/mL), IL-6 (from 63.1 to 77.3 pg/mL), and IL-10 (from 48.9 to 59.6 pg/mL) 1 h after the end of the running protocol. However, the curcumin and piperine supplementation could inhibit this elevation. Conclusions: curcumin and piperine supplementation had no effect on physical performance, immune cell counts, or muscle damage; however, the supplementation could modulate the kinetics of IL-2, TNF-α, INF, IL-6, and IL-10 1 h after the end of exercise.9E1A-F9DD-3EB8 | Filipe Manuel ClementeN/

Rejeito de barragem de min?rio de ferro na produ??o de ladrilhos hidr?ulicos : uma an?lise de valor para sustentabilidade de edifica??es.

The present work discloses the development of a sustainable cement tile (SCT) produced with Iron Ore Tailings from tailings dams (IOT). Initially, technical evaluation and environmental analysis of IOT were performed through physical, chemical and morphological characterisation, leaching and dissolution tests. Its feasibility as a pigment was also investigated. Subsequently, the value analysis was performed, starting from an empathy map to a study on environmental, social and emotional values in the relationship between people and product. As result, the IOT presented fine, crystalline particles, no toxicity, and is technically feasible to be employed as filler, aggregate and pigment in the production of SCT. The colour layer of the SCT presented more homogeneous colour and less pores than conventional ones. Therefore, the design of the SCT was developed, incorporating intangible values such as: environmental ideology, local identity and social expression. In this sense, the present work seeks to assist in the decision-making process involving IOT as construction material.O presente trabalho abrange o desenvolvimento de ladrilho hidr?ulico sustent?vel (LHS) produzido com rejeitos de barragem de min?rio de ferro (RBMF). Inicialmente, a avalia??o t?cnica e a an?lise ambiental do RBMF foram realizadas atrav?s de testes de caracteriza??o f?sica, qu?mica e morfol?gica, lixivia??o e dissolu??o. Sua viabilidade como pigmento tamb?m foi investigada. Posteriormente, a an?lise de valor foi realizada, partindo de um mapa de empatia at? o estudo sobre valores ambientais, sociais e emocionais na rela??o entre pessoas e produtos. Como resultado, o RBMF se apresentou como part?culas finas, cristalinas, sem toxicidade, sendo tecnicamente vi?vel para ser empregado como f?ler, agregado e pigmento na produ??o de LHS. A camada de cor do LHS apresentou cores mais homog?neas e menos poros do que as convencionais. Assim, o projeto do LHS foi desenvolvido, incorporando valores intang?veis como: ideologia ambiental, identidade local e express?o social. Nesse sentido, o presente trabalho busca ajudar nas tomadas de decis?o envolvendo RBMF como material de constru??o

description of the methodological approach

publishersversionpublishe