Low-rate discharge of various electrochemical batteries for use with oceanographic instruments

The endurance of self-sustained oceanographic instruments is generally limited to battery energy. Tests were initiated to measure the capacities of several types of electrochemical batteries when discharged at temperatures and rates typical of oceanographic use. Battery systems represented are alkaline-manganese dioxide, mercuric-oxide, and lithium sulphur oxychloride. Results of tests completed so far are presented. A brief overview of those batteries best suited for use with self-sustained oceanographic instruments is included as an appendix.Funding was provided by the Office of Naval Research under contract Number N00014-76-C-0197, NR 083-400

Middle to late Cenozoic basin evolution in the western Alborz Mountains: Implications for the onset of collisional deformation in northern Iran

Oligocene-Miocene strata preserved in synclinal outcrop belts of the western Alborz Mountains record the onset of Arabia-Eurasia collision-related deformation in northern Iran. Two stratigraphic intervals, informally named the Gand Ab and Narijan units, represent a former basin system that existed in the Alborz. The Gand Ab unit is composed of marine lagoonal mudstones, fluvial and alluvial-fan clastic rocks, fossiliferous Rupelian to Burdigalian marine carbonates, and basalt flows yielding ^(40)Ar/^(39)Ar ages of 32.7 ± 0.3 and 32.9 ± 0.2 Ma. The Gand Ab unit is correlated with the Oligocene–lower Miocene Qom Formation of central Iran and is considered a product of thermal subsidence following Eocene extension. The Narijan unit unconformably overlies the Gand Ab unit and is composed of fluvial-lacustrine and alluvial fan sediments exhibiting contractional growth strata. We correlate the Narijan unit with the middle to upper Miocene Upper Red Formation of central Iran on the basis of lithofacies similarities, stratigraphic position, and an 8.74 ± 0.15 Ma microdiorite dike (^(40)Ar/^(39)Ar) that intruded the basal strata. Deformation timing is constrained by crosscutting relationships and independent thermochronological data. The Parachan thrust system along the eastern edge of the ancestral Taleghan-Alamut basin is cut by dikes dated at 8.74 ± 0.15 Ma to 6.68 ± 0.07 Ma (^(40)Ar/^(39)Ar). Subhorizontal gravels that unconformably overlie tightly folded and faulted Narijan strata are capped by 2.86 ± 0.83 Ma (^(40)Ar/^(39)Ar) andesitic lava flows. These relationships suggest that Alborz deformation had migrated southward into the Taleghan-Alamut basin by late Miocene time and shifted to its present location along the active range front by late Pliocene time. Data presented here demonstrate that shortening in the western Alborz Mountains had started by late middle Miocene time. This estimate is consistent with recent thermochronological results that place the onset of rapid exhumation in the western Alborz at ∼12 Ma. Moreover, nearly synchronous Miocene contraction in the Alborz, Zagros Mountains, Turkish-Iranian plateau, and Anatolia suggests that the Arabia-Eurasia collision affected a large region simultaneously, without a systematic outward progression of mountain building away from the collision zone

Brazil Basin Tracer Release Experiment

The purpose of the Brazil Basin Tracer Release Experiment is to measure diapycnal (across isopycnal) mixing and epipycnal (along-isopycnal) mixing and stirring in the deep ocean. This cruise is the fourth in the overall experiment. In the first cruise in early 1996, 110 kg of sulfur hexafluoride (SF6) were released on an isopycnal surface near 4000 meters depth in the eastern part of the basin on the flanks of the Mid-Atlantic Ridge (MAR). The location of the release was near 21.7 S, 18.4 W. The release site was over a zonal valley that leads to the MAR and is about 5000 m deep. The isopycnal surface of the release was defined as the surface on which the potential density anomaly, referenced to 4000 dbar pressure, was 45.9408 kg/m3. The release streaks and results of initial sampling in 1996 are described in Polzin et al. [1997]

An X-ray Proper Motion Study of the LMC SNR 0509-67.5

We present a third epoch of Chandra observations of the Type Ia Large Magellanic Cloud Supernova remnant (SNR) 0509-67.5. With these new observations from 2020, the baseline for proper motion measurements of the expansion has grown to 20 years (from the earliest Chandra observations in 2000). We report here the results of these new expansion measurements. The lack of nearby bright point sources renders absolute image alignment difficult. However, we are able to measure the average expansion of the diameter of the remnant along several projection directions. We find that the remnant is expanding with an average velocity of 6120 (4900 -- 7360) km s$^{-1}$. This high shock velocity is consistent with previous works, and also consistent with the inference that 0509-67.5 is expanding into a very low density surrounding medium. At the distance of the LMC, this velocity corresponds to an undecelerated age of 600 yrs, with the real age somewhat smaller.Comment: 9 pages, 5 figures. Accepted for publication in Ap

Shearmeter floats in the area of the WHOI Brazil Basin Tracer Release Experiment : technical and oceanographic data

Six drifting floats designed to measure shear were deployed in the vicinity of the Brazil Basin Tracer Release Experiment. The one-year long time series of oceanographic conditions obtained by the floats are for direct comparison with long-term tracer dispersion. The purpose of the tracer dispersion experiment was to study mixing of Antarctic Bottom Water at approximately 4000 m depth with less dense water above. Two of the floats returned shear records, one from about 1660 m depth and one from about 2800 m depth. Mean shear at 1660 m was 2.2 x 10 -3 s-1 with N = 1.1 cph, about 1.9 times the Garrett-Munk model amount. Mean shear at 2800 m was 1.1 x 10-3 with N = 0.5 cph, about 2.2 times Garrett-Munk. There was no apparent depth structure to the shear recorded by the near-bottom float moving over the mountainous seafloor. The two shear time series and the local tidal velocities were not strongly correlated, but the tide and shear series did have some similarities. Some variability in the 1660-m shear may be due to atmospheric forcing. Three floats deeper than 2800 m returned one-year long trajectories. Two trajectories were persistently eastward.Funding was provided by the National Science Foundation under Grant Nos. OCE-9416014 and OCE-9906685

Preliminary imaging results from the second Mercury encounter

The second Mercury encounter has resulted in the acquisition of about 360 pictures of the south polar regions which provide a reliable cartographic and geologic tie between the two sides of the planet photographed on the first encounter. Stereoscopic coverage of large areas of the southern hemisphere was obtained by combining Mercury 1 and 2 pictures taken at different viewing angles. The south polar regions consist of heavily cratered terrain and intercrater plains interspersed with patches of smooth plains. No large areas of smooth plains similar to those surrounding Caloris occur in the south polar regions. No new types of terrain have been recognized, but lobate scarps are common. The second largest basin seen by Mariner 10 (∼600-km diameter) has been confirmed on the new photography. At high solar elevations the surface displays an abundance of rays and rayed craters

Unwinding of primer-templates by archaeal family-B DNA polymerases in response to template-strand uracil

Archaeal family-B DNA polymerases bind tightly to deaminated bases and stall replication on encountering uracil in template strands, four bases ahead of the primer-template junction. Should the polymerase progress further towards the uracil, for example, to position uracil only two bases in front of the junction, 3′–5′ proof-reading exonuclease activity becomes stimulated, trimming the primer and re-setting uracil to the +4 position. Uracil sensing prevents copying of the deaminated base and permanent mutation in 50% of the progeny. This publication uses both steady-state and time-resolved 2-aminopurine fluorescence to show pronounced unwinding of primer-templates with Pyrococcus furiosus (Pfu) polymerase–DNA complexes containing uracil at +2; much less strand separation is seen with uracil at +4. DNA unwinding has long been recognized as necessary for proof-reading exonuclease activity. The roles of M247 and Y261, amino acids suggested by structural studies to play a role in primer-template unwinding, have been probed. M247 appears to be unimportant, but 2-aminopurine fluorescence measurements show that Y261 plays a role in primer-template strand separation. Y261 is also required for full exonuclease activity and contributes to the fidelity of the polymerase

Antigenicity and immunogenicity of differentially glycosylated HCV E2 envelope proteins expressed in mammalian and insect cells

Development of a prophylactic vaccine for hepatitis C virus (HCV) remains a global health challenge. Cumulative evidence supports the importance of antibodies targeting the HCV E2 envelope glycoprotein to facilitate viral clearance. However, a significant challenge for a B cell-based vaccine is focusing the immune response on conserved E2 epitopes capable of eliciting neutralizing antibodies not associated with viral escape. We hypothesized that glycosylation might influence the antigenicity and immunogenicity of E2. Accordingly, we performed head-to-head molecular, antigenic and immunogenic comparisons of soluble E2 (sE2) produced in (i) mammalian (HEK293) cells, which confer mostly complex and high mannose type glycans; and (ii) insect (Sf9) cells, which impart mainly paucimannose type glycans. Mass spectrometry demonstrated that all 11 predicted N-glycosylation sites were utilized in both HEK293- and Sf9-derived sE2, but that N-glycans in insect sE2 were on average smaller and less complex. Both proteins bound CD81 and were recognized by conformation-dependent antibodies. Mouse immunogenicity studies revealed that similar polyclonal antibody responses were generated against antigenic domains A–E of E2. Although neutralizing antibody titers showed that Sf9-derived sE2 induced moderately stronger responses than HEK293-derived sE2 against the homologous HCV H77c isolate, the two proteins elicited comparable neutralization titers against heterologous isolates. Given that global alteration of HCV E2 glycosylation by expression in different hosts did not appreciably affect antigenicity or overall immunogenicity, a more productive approach to increasing the antibody response to neutralizing epitopes may be complete deletion, rather than just modification, of specific N-glycans proximal to these epitopes

Human Neural Stem Cells Differentiate and Promote Locomotor Recovery in an Early Chronic Spinal coRd Injury NOD-scid Mouse Model

Traumatic spinal cord injury (SCI) results in partial or complete paralysis and is characterized by a loss of neurons and oligodendrocytes, axonal injury, and demyelination/dysmyelination of spared axons. Approximately 1,250,000 individuals have chronic SCI in the U.S.; therefore treatment in the chronic stages is highly clinically relevant. Human neural stem cells (hCNS-SCns) were prospectively isolated based on fluorescence-activated cell sorting for a CD133(+) and CD24(-/lo) population from fetal brain, grown as neurospheres, and lineage restricted to generate neurons, oligodendrocytes and astrocytes. hCNS-SCns have recently been transplanted sub-acutely following spinal cord injury and found to promote improved locomotor recovery. We tested the ability of hCNS-SCns transplanted 30 days post SCI to survive, differentiate, migrate, and promote improved locomotor recovery.hCNS-SCns were transplanted into immunodeficient NOD-scid mice 30 days post spinal cord contusion injury. hCNS-SCns transplanted mice demonstrated significantly improved locomotor recovery compared to vehicle controls using open field locomotor testing and CatWalk gait analysis. Transplanted hCNS-SCns exhibited long-term engraftment, migration, limited proliferation, and differentiation predominantly to oligodendrocytes and neurons. Astrocytic differentiation was rare and mice did not exhibit mechanical allodynia. Furthermore, differentiated hCNS-SCns integrated with the host as demonstrated by co-localization of human cytoplasm with discrete staining for the paranodal marker contactin-associated protein.The results suggest that hCNS-SCns are capable of surviving, differentiating, and promoting improved locomotor recovery when transplanted into an early chronic injury microenvironment. These data suggest that hCNS-SCns transplantation has efficacy in an early chronic SCI setting and thus expands the "window of opportunity" for intervention