Diseño de material educativo. Aplicación para trabajar la morfosintaxis

Trabajo Fin de Grado. Grado en Logopedia. Curso Académico 2013/201

Composición química de la carne de conejo silvestre (Oryctolagus cuniculus) y viabilidad de su predicción mediante espectroscopía de infrarrojo cercano

En algunos países del entorno mediterráneo existe tradición de consumo de carne de conejo silvestre procedente de la caza. Sin embargo, las características de la canal y de la carne de esta especie cinegética han sido escasamente investigadas. Los objetivos de este estudio fueron analizar la composición química de la carne de conejo silvestre (Oryctolagus cuniculus algirus) procedente del sudoeste de la Península Ibérica y estudiar la viabilidad de la puesta a punto de una metodología rápida de análisis para su determinación mediante espectroscopía de infrarrojo cercano (NIRS). La carne de conejo silvestre de la subespecie O. c. algirus contiene un 23,7% de proteína bruta, 0,2% de grasa bruta, 74,9% de humedad y 1,2% de cenizas. Esta composición difiere de la publicada para conejos domésticos y para conejos silvestres de la subespecie O. c. cuniculus, siendo más magra debido a su mayor contenido de proteína y muy inferior valor de grasa. Se obtuvieron modelos basados en la espectroscopía NIR con buena capacidad de predicción para los parámetros proteína y humedad (r2 = 0,70 y ETVC = 0,39%; y r2 = 0,73 y ETVC = 0,38%; respectivamente); mientras que las calibraciones seleccionadas para grasa y cenizas no resultaron ser aceptables.Wild rabbit meat is commonly consumed in many Mediterranean countries. However, the characteristics of the carcass and meat of this game species have been scarcely investigated. This study was aimed at analysing the chemical composition of hunted wild rabbit (Oryctolagus cuniculus algirus) meat from Southern Iberian Peninsula, and studying the viability of a rapid analytical methodology for its determination by near infrared spectroscopy. Meat of the wild rabbit subspecies O. c. algirus has 23.7% of crude protein, 0.2% of crude fat, 74.9% of moisture and 1.2% of ash. This composition differed from the values published for domesticated rabbits and for wild rabbits of the O. c. cuniculus subspecies, it being leaner due to its higher protein content and its very lower fat content. NIR spectroscopy models obtained displayed a good predictive ability for the estimation of crude protein and moisture contents (r2 = 0.70 and SECV = 0.39%, and r2 = 0.73 and SECV = 0.38%, respectively), while the calibrations selected for crude fat and ash contents were not acceptabl

La morfosintaxis con tel expressivo. Aplicaciones multimedia como propuesta de intervención logopédica: modelo assure

Las dificultades en tareas de morfosintaxis, pueden presentarse como parámetros a ser intervenidos en distintas dificultades, tales como Retraso Simple del Lenguaje (RL), Trastorno Específico del Lenguaje (TEL; el concepto hace referencia a un conjunto de dificultades en la adquisición del lenguaje que están presentes en un grupo de niños que no evidencian problemas neurológicos, cognitivos, sensoriales, motores ni sociofamiliares), mientras que en el Trastorno por Déficit de Atención e Hiperactividad (TDAH), Déficit Auditivos, Trastorno del Espectro Autista (TEA), Síndromes genéticos, etc., aparece como síntoma asociado a su diagnóstico.A lo largo de este artículo se presenta la creación de una App, cuya finalidad general de la misma, gira a ser una posible solución que pueda llegar a solventar la problemática existente, trabajar el área de la morfosintaxis con un caso diagnosticado con TEL expresivo. Para la creación de esta App, nos hemos basado en el modelo ASSURE, un sistema de diseño instruccional, que los profesionales tanto em la didáctica como em laogopedia pueden usar para diseñar y desarrollar un ambiente de enseñanza-aprendizaje. Inicidimos em que la App que se presenta a lo largo del artículo, aunque en rasgos generales puede llegar a ser aplicada a diferentes casos, es diseñada y puesta en práctica para un sólo caso

Automation of an atomic force microscope via Arduino

The Dimension 3000 AFM used in this work was kindly donated by Prof. Nicholas D. Spencer, and facilitated by Prof. Lucio Isa, and Dr. Shivaprakash N. Ramakrishna, from ETH-Zurich. We thank Prof. David Cuartielles for encouraging us to publish this work in this special issue on Arduino Science Hardware. We also thank Llorenc Mercadal Fernandez for frutiful discussions and ideas, and the BiblioMaker unit in the Faculty of Sciences of the University of Granada for their help in 3D printing the gears used here. MAFR acknowledges support by the project PID2020-116615RA-I00 funded by MCIN/AEI/10.13039/501100011033, and the EMERGIA grant with reference EMC21_00008 funded by Consejeria de Universidad, Investigacion e Innovacion de la Junta de Andalucia, and by FEDER "ERDF A way of making Europe". JGGF and CLMM acknowledge support from grant A1S35536 by Conacyt Mexico.The Atomic Force Microscopy is a very versatile technique that allows to characterize surfaces by acquiring topographies with sub-nanometer resolution. This technique often overcomes the problems and capabilities of electron microscopy when characterizing few nanometers thin coatings over solid substrates. They are expensive, in the half million dollar range for standard units, and therefore it is often difficult to upgrade to new units with improved characteristics. One of these improvements, motorization and automation of the measurements is very interesting to sample different parts of a substrate in an unattended way. Here we report a low cost upgrade under 60 $ to a Dimension 3000 AFM based on a control unit using an Arduino Leonardo. It enables to acquire dozens or hundreds of images automatically by mimicking keyboard shortcuts and interfacing the AFM PCI card.MCIN/AEI PID2020-116615RA-I00Consejeria de Universidad, Investigacion e Innovacion de la Junta de Andalucia EMC21_00008Marie Curie ActionsConsejo Nacional de Ciencia y Tecnologia (CONACyT) A1S3553

Lysine 27 dimethylation of Drosophila linker histone dH1 contributes to heterochromatin organization independently of H3K9 methylation

Post-translational modifications (PTMs) of core histones are important epigenetic determinants that correlate with functional chromatin states. However, despite multiple linker histone H1s PTMs have been identified, little is known about their genomic distribution and contribution to the epigenetic regulation of chromatin. Here, we address this question in Drosophila that encodes a single somatic linker histone, dH1. We previously reported that dH1 is dimethylated at K27 (dH1K27me2). Here, we show that dH1K27me2 is a major PTM of Drosophila heterochromatin. At mitosis, dH1K27me2 accumulates at pericentromeric heterochromatin, while, in interphase, it is also detected at intercalary heterochromatin. ChIPseq experiments show that >98% of dH1K27me2 enriched regions map to heterochromatic repetitive DNA elements, including transposable elements, simple DNA repeats and satellite DNAs. Moreover, expression of a mutated dH1K27A form, which impairs dH1K27me2, alters heterochromatin organization, upregulates expression of heterochromatic transposable elements and results in the accumulation of RNA:DNA hybrids (R-loops) in heterochromatin, without affecting H3K9 methylation and HP1a binding. The pattern of dH1K27me2 is H3K9 methylation independent, as it is equally detected in flies carrying a H3K9R mutation, and is not affected by depletion of Su(var)3–9, HP1a or Su(var)4–20. Altogether these results suggest that dH1K27me2 contributes to heterochromatin organization independently of H3K9 methylation.MICIN/AEI 10.13039/501100011033 [BFU2015-65082-P and PGC2018-094538-B-100]; ‘FEDER, una manera de hacer Europa’; Generalitat de Catalunya [SGR2014-204, SGR2017-475]; this work was carried out within the framework of the ‘Centre de Referencia en Biotecnologia’ of ` the Generalitat de Catalunya. Funding for open access charge: MINECO [PGC2018-094538-B-100]. Conflict of interest statement. None declared

Direct X-Ray detection of the spin Hall effect in CuBi

The spin Hall effect and the inverse spin Hall effect are important spin-charge conversion mechanisms. The direct spin Hall effect induces a surface spin accumulation from a transverse charge current due to spin-orbit coupling even in nonmagnetic conductors. However, most detection schemes involve additional interfaces, leading to large scattering in reported data. Here we perform interface-free x-ray spectroscopy measurements at the Cu L3,2 absorption edges of highly Bi-doped Cu (Cu95Bi5). The detected x-ray magnetic circular dichroism signal corresponds to an induced magnetic moment of (2.2 ± 0.5) × 10-12 μB A-1 cm2 per Cu atom averaged over the probing depth, which is of the same order of magnitude as found for Pt measured by magneto-optics. The results highlight the importance of interface-free measurements to assess material parameters and the potential of CuBi for spin-charge conversion application

Current Perspectives on the Use of Anti-VEGF Drugs as Adjuvant Therapy in Glaucoma

The approval of one of the first anti-vascular endothelial growth factor (VEGF) agents for the treatment of neovascular age-related macular degeneration one decade ago marked the beginning of a new era in the management of several sight-threatening retinal diseases. Since then, emerging evidence has demonstrated the utility of these therapies for the treatment of other ocular conditions characterized by elevated VEGF levels. In this article we review current perspectives on the use of anti-VEGF drugs as adjuvant therapy in the management of neovascular glaucoma (NVG). The use of anti-VEGFs for modifying wound healing in glaucoma filtration surgery (GFS) is also reviewed. Selected studies investigating the use of anti-VEGF agents or antimetabolites in GFS or the management of NVG have demonstrated that these agents can improve surgical outcomes. However, anti-VEGF agents have yet to demonstrate specific advantages over the more established agents commonly used today. Further studies are needed to evaluate the duration of action, dosing intervals, and toxicity profile of these treatments

A specific gut microbiota signature is associated with an enhanced GLP-1 and GLP-2 secretion and improved metabolic control in patients with type 2 diabetes after metabolic Roux-en-Y gastric bypass

Objective: To determine changes in incretins, systemic inflammation, intestinal permeability and microbiome modifications 12 months after metabolic RYGB (mRYGB) in patients with type 2 diabetes (T2D) and their relationship with metabolic improvement. Materials and methods: Prospective single-center non-randomized controlled study, including patients with class II-III obesity and T2D undergoing mRYGB. At baseline and one year after surgery we performed body composition measurements, biochemical analysis, a meal tolerance test (MTT) and lipid test (LT) with determination of the area under the curve (AUC) for insulin, C-peptide, GLP-1, GLP-2, and fasting determinations of succinate, zonulin, IL-6 and study of gut microbiota. Results: Thirteen patients aged 52.6 ± 6.5 years, BMI 39.3 ± 1.4 kg/m2, HbA1c 7.62 ± 1.5% were evaluated. After mRYGB, zonulin decreased and an increase in AUC after MTT was observed for GLP-1 (pre 9371 ± 5973 vs post 15788 ± 8021 pM, P<0.05), GLP-2 (pre 732 ± 182 vs post 1190 ± 447 ng/ml, P<0.001) and C- peptide, as well as after LT. Species belonging to Streptococaceae, Akkermansiacea, Rickenellaceae, Sutterellaceae, Enterobacteriaceae, Oscillospiraceae, Veillonellaceae, Enterobacterales_uc, and Fusobacteriaceae families increased after intervention and correlated positively with AUC of GLP-1 and GLP-2, and negatively with glucose, HbA1c, triglycerides and adiposity markers. Clostridium perfringens and Roseburia sp. 40_7 behaved similarly. In contrast, some species belonging to Lachnospiraceae, Erysipelotricaceae, and Rumnicocaceae families decreased and showed opposite correlations. Higher initial C-peptide was the only predictor for T2D remission, which was achieved in 69% of patients. Conclusions: Patients with obesity and T2D submitted to mRYGB show an enhanced incretin response, a reduced gut permeability and a metabolic improvement, associated with a specific microbiota signature

Direct x-ray detection of the spin hall effect in CuBi

The spin Hall effect and the inverse spin Hall effect are important spin-charge conversion mechanisms. The direct spin Hall effect induces a surface spin accumulation from a transverse charge current due to spin-orbit coupling even in nonmagnetic conductors. However, most detection schemes involve additional interfaces, leading to large scattering in reported data. Here we perform interface-free x-ray spectroscopy measurements at the Cu L_(3;2) absorption edges of highly Bi-doped Cu (Cu_(95)Bi_5). The detected x-ray magnetic circular dichroism signal corresponds to an induced magnetic moment of (2.2 + 0.5) x 10^(-12) mu(B) A^(-1) cm^(2) per Cu atom averaged over the probing depth, which is of the same order of magnitude as found for Pt measured by magneto-optics. The results highlight the importance of interface-free measurements to assess material parameters and the potential of CuBi for spin-charge conversion applications