47 research outputs found
Functional analysis of the sporulation-specific SPR6 gene of Saccharomyces cerevisiae
Full text linkThe SPR6 gene of Saccharomyces cerevisiae encodes a moderately abundant RNA that is present at high levels only during sporulation. The gene contains a long open reading frame that could encode a hydrophilic protein approximately 21 kDa in size. This protein is probably produced by the yeast, because the lacZ gene of Escherichia coli is expressed during sporulation when fused to SPR6 in the expected reading frame. SPR6 is inessential for sporulation; mutants that lack SPR6 activity sporulate normally and produce viable ascospores. Nonetheless, the SPR6 gene encodes a function that is relevant to sporulating cells; the wild-type allele can enhance sporulation in strains that are defective for several SPR functions. SPR6 is located on chromosome V, 14.4 centimorgans centromere-distal to MET6 .Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/46973/1/294_2004_Article_BF00318210.pd
Optimal gene expression and amplification strategies for batch and continuous recombinant cultures
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Use of transcription and translation signals from the colicin El gene to express DNA sequences in Escherichia coli
No full textMolecular design of expression systems: Comparison of different control configurations using molecular mechanism models
No full textPorphyrin biosynthesis in normal and haem mutants of Saccharomyces cerevisiae. Studies on the inheritance of the HEM R+ phenotype
No full textNon-linear optimization of biotechnological processes by stochastic algorithms: Application to the maximization of the production rate of ethanol, glycerol and carbohydrates by Saccharomyces cerevisiae
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New versatile plasmid vectors for expression of hybrid proteins coded by a cloned gene fused to lacA gene sequences encoding an enzymatically active carboxy-terminal portion of β-galactosidase
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