Investigating self-recognition in bonobos : mirror exposure reduces looking time to self but not unfamiliar conspecifics

The research leading to these results has received funding from the European Research Council under the European Union's Seventh Framework Programme (FP7/2007-2013)/ERC grant agreement no. 283871 and the Swiss National Science Foundation (Social learning in primate communication: 31003A_166458 / Coordinating joint action in apes: Testing the boundaries of the human interaction engine: CR31I3_159655).The question of whether animals have some sort ofself-awareness is a topic of continued debate. A necessary precondition forself-awareness is the ability to visually discriminate the self from others,which has traditionally been investigated through mirror self-recognition experiments.Although great apes generally pass such experiments, interpretations of resultshave remained controversial. The aim of this study was to investigate howbonobos (Pan paniscus) respond todifferent types of images of themselves and others, both before and afterprolonged mirror exposure. We first presented presumably mirror-naive subjectswith representations of themselves in three different ways (mirror image,contingent and non-contingent video footage) as well as representations ofothers (video footage of known and unknown conspecifics). We found thatsubjects paid significantly less attention to contingent images of themselves(mirror image, video footage) than to non-contingent images of themselves andunfamiliar individuals, suggesting they perceived the non-contingent self-imageas novel. We then provided subjects with three months of access to a largemirror centrally positioned in the enclosure. Following this manipulation,subjects showed significantly reduced interest in the non-contingentself-images, while interest in unknown individuals remained unchanged,suggesting that the mirror experience has led to a fuller understanding oftheir own self. We discuss implications of this preliminary investigation forthe on-going debate on self-awareness in animals.Publisher PDFPeer reviewe

Non-Equilibrium Ionic Liquid-Electrode Interface at Elevated Temperature and Its Influence on Co2+Reduction Process

Electrodeposition in Ionic Liquid (IL) media is still at its infancy stage. Recently much attention is devoted to exploration of the IL-electrode interface for better understanding of the electrochemical processes at the electrified surfaces. Herein, we provide a new perspective of the temperature-driven IL-substrate interface structuring. We observed an increase in Co2+ reduction kinetics induced by applying a high temperature gradient. In contrast, keeping the cell at a high constant temperature (100°C) leads to slower Co2+ reduction kinetics, it results in a shift of the reduction onset and evolution of the cyclic voltammogram shape with time. We could ascribe these phenomena to the disturbance of the electrochemical double layer (induced by heating) and its further slow reorganization at constant temperature. We showed that such non-equilibrium behavior can be beneficial for the synthesis of metallic thin films from ILs. Electrochemical quartz crystal microgravimetry, impedance spectroscopy, cyclic voltammetry and chronoamperometry were employed within this work

adaptation across biologically relevant ‘levels’ in tamarins

The article processing charge was funded by the Open Access Publication Fund of Humboldt-Universität zu Berlin.Background: Biological adaptation manifests itself at the interface of different biologically relevant ‘levels’, such as ecology, performance, and morphology. Integrated studies at this interface are scarce due to practical difficulties in study design. We present a multilevel analysis, in which we combine evidence from habitat utilization, leaping performance and limb bone morphology of four species of tamarins to elucidate correlations between these ‘levels’. Results: We conducted studies of leaping behavior in the field and in a naturalistic park and found significant differences in support use and leaping performance. Leontocebus nigrifrons leaps primarily on vertical, inflexible supports, with vertical body postures, and covers greater leaping distances on average. In contrast, Saguinus midas and S. imperator use vertical and horizontal supports for leaping with a relatively similar frequency. S. mystax is similar to S. midas and S. imperator in the use of supports, but covers greater leaping distances on average, which are nevertheless shorter than those of L. nigrifrons. We assumed these differences to be reflected in the locomotor morphology, too, and compared various morphological features of the long bones of the limbs. According to our performance and habitat utilization data, we expected the long bone morphology of L. nigrifrons to reflect the largest potential for joint torque generation and stress resistance, because we assume longer leaps on vertical supports to exert larger forces on the bones. For S. mystax, based on our performance data, we expected the potential for torque generation to be intermediate between L. nigrifrons and the other two Saguinus species. Surprisingly, we found S. midas and S. imperator having relatively more robust morphological structures as well as relatively larger muscle in-levers, and thus appearing better adapted to the stresses involved in leaping than the other two. Conclusion: This study demonstrates the complex ways in which behavioral and morphological ‘levels’ map onto each other, cautioning against oversimplification of ecological profiles when using large interspecific eco-morphological studies to make adaptive evolutionary inferences.Peer Reviewe

Enduring voice recognition in bonobos

We would like to thank the French Ministère de l’Enseignement Supérieur et de la Recherche (PhD grant to SK), the Université de Saint-Etienne (research sabbaticals to FL and NM, visiting professorship to KZ and research funding) and the European Research Council (KZ grant PRILANG 283871).Long-term social recognition is vital for species with complex social networks, where familiar individuals can encounter one another after long periods of separation. For non-human primates who live in dense forest environments, visual access to one another is often limited, and recognition of social partners over distances largely depends on vocal communication. Vocal recognition after years of separation has never been reported in any great ape species, despite their complex societies and advanced social intelligence. Here we show that bonobos, Pan paniscus, demonstrate reliable vocal recognition of social partners, even if they have been separated for five years. We experimentally tested bonobos' responses to the calls of previous group members that had been transferred between captive groups. Despite long separations, subjects responded more intensely to familiar voices than to calls from unknown individuals - the first experimental evidence that bonobos can identify individuals utilising vocalisations even years after their last encounter. Our study also suggests that bonobos may cease to discriminate between familiar and unfamiliar individuals after a period of eight years, indicating that voice representations or interest could be limited in time in this species.Publisher PDFPeer reviewe

Electrochemical Method for Direct Deposition of Nanometric Bismuth and Its Electrochemical Properties vs Li

We report that nanometric bismuth can directly be electrodeposited at room temperature without the use of a nanoporous template. The morphology, microstructure, and purity of the as-prepared electrodeposits were characterized by scanning electron microscopy, transmission electron microscopy, and infrared spectroscopy. Typically, well-crystallized nanometer-sized particles of Bi ranging from 10 to 20 nm are obtained. The key to success of such a process lies in the electrochemical coreduction of pyrocatechol violet during the bismuth deposition, which disturbs the electrocrystallization process. As a first possible application, we show that Bi/Cu nanoarchitectured electrodes exhibit interesting rate capabilities when used as electrode material vs Li

Collective oscillations of a classical gas confined in harmonic traps

Starting from the Boltzmann equation we calculate the frequency and the damping of the monopole and quadrupole oscillations of a classical gas confined in an harmonic potential. The collisional term is treated in the relaxation time approximation and a gaussian ansatz is used for its evaluation. Our approach provides an explicit description of the transition between the hydrodynamic and collisionless regimes in both spherical and deformed traps. The predictions are compared with the results of a numerical simulation.Comment: 6 pages, revtex, 2 figures include

Importance of the double layer structure in the electrochemical deposition of Co from soluble Co2+ - based precursors in Ionic Liquid media

Electrodeposition in Ionic Liquid (IL) media is being widely used to obtain different metals, alloys and more recently binary compounds. However, the understanding of this process is still slim due to poor knowledge in the chemistry of ILs and also the complex structure of the double layer at the electrode surface. It is then difficult to predict deposition conditions when trying to synthesize a desired deposit. In this work, we provide insight into the processes taking place at the electrode by detailed studies of Co2+ reduction in ionic liquid media at elevated temperature, which have revealed an unusual Cyclic Voltammetry profile. A drastic drop in the reaction rate was observed, together with a well-shaped reduction peak during the oxidation sweep. Using Electrochemical Quartz Crystal Microbalance, Impedance Spectroscopy measurements and varying bath parameters, we ascribed those phenomena to the reconstruction of the double layer structure upon imposed polarization. Similar behaviors were observed for other systems (Mn2+, Ni2+, Zn2+). Finally, this work provides new insight into the electrochemical processes in ILs. We anticipate that it will also result in significant improvement in preparing metal and other deposits in the ionic liquid media

Intestinal Protists in Captive Non-human Primates and Their Handlers in Six European Zoological Gardens. Molecular Evidence of Zoonotic Transmission

We assessed the occurrence, genetic diversity, and zoonotic potential of four protozoan (Cryptosporidium spp., Entamoeba histolytica, Entamoeba dispar, Giardia duodenalis), one stramenopile (Blastocystis sp.), one microsporidia (Enterocytozoon bieneusi), and two ciliate (Balantioides coli, Troglodytella abrassarti) intestinal parasite or commensal protist species in captive non-human primates (NHP) and their zookeepers from six European zoological gardens in France (n = 1), Germany (n = 1), and Spain (n = 4). Faecal samples from NHP (n = 454) belonging to 63 species within 35 genera and humans (n = 70) were collected at two sampling periods in each participating institution between October 2018-August 2021. Detection and species identification was accomplished by PCR and Sanger sequencing of the ssu rRNA and/or ITS genes. Sub-genotyping analyses using specific markers were conducted on isolates positive for G. duodenalis (gdh, bg, tpi) and Cryptosporidium spp. (gp60). Overall, 41.0% (186/454) and 30.0% (21/70) of the faecal samples of NHP and human origin tested positive for at least one intestinal protist species, respectively. In NHP, Blastocystis sp. was the most prevalent protist species found (20.3%), followed by G. duodenalis (18.1%), E. dispar (7.9%), B. coli and T. abrassarti (1.5% each), and Cryptosporidium spp. and E. bieneusi (0.9% each). Occurrence rates varied largely among NHP host species, sampling periods, and zoological institutions. The predominant protist species found in humans was Blastocystis sp. (25.7%), followed by Cryptosporidium spp. (2.9%), E. dispar (1.4%), and G. duodenalis (1.4%). Sequencing of PCR-positive amplicons in human and/or NHP confirmed the presence of Cryptosporidium in six isolates (C. hominis: 66.7%, C. parvum: 33.3%), G. duodenalis in 18 isolates (assemblage A: 16.7%, assemblage B: 83.3%), Blastocystis in 110 isolates (ST1:38.2%, ST2:11.8%, ST3: 18.2%, ST4: 9.1%, ST5: 17.3%, ST8: 2.7%, ST13: 0.9%), and E. bieneusi in four isolates (CM18: 75.0%, Type IV: 25.0%). Zoonotic transmission events involving Blastocystis ST1-ST4 were identified in four zoological institutions. Zoonotic transmission of C. hominis was highly suspected, but not fully demonstrated, in one of them. Monitoring of intestinal protist species might be useful for assessing health status of captive NHP and their zookeepers, and to identify transmission pathways of faecal-orally transmitted pathogens.This study was funded by the Health Institute Carlos III (ISCIII), Spanish Ministry of Economy and Competitiveness under project PI16CIII/00024. DG-B was recipient of a Sara Borrell Postdoctoral Fellowship (CD19CIII/00011) funded by the Spanish Ministry of Science, Innovation and Universities. AD was recipient of a PFIS contract (FI20CIII/00002) funded by the Spanish Ministry of Science and Innovation and Universities.S