The effect of UV-B on Arabidopsis leaves depends on light conditions after treatment

BACKGROUND: Ultraviolet B (UV-B) irradiation can influence many cellular processes. Irradiation with high UV-B doses causes chlorophyll degradation, a decrease in the expression of genes associated with photosynthesis and its subsequent inhibition. On the other hand, sublethal doses of UV-B are used in post-harvest technology to prevent yellowing in storage. To address this inconsistency the effect of short, high-dose UV-B irradiation on detached Arabidopsis thaliana leaves was examined. RESULTS: Two different experimental models were used. After short treatment with a high dose of UV-B the Arabidopsis leaves were either put into darkness or exposed to constant light for up to 4 days. UV-B inhibited dark-induced chlorophyll degradation in Arabidopsis leaves in a dose-dependent manner. The expression of photosynthesis-related genes, chlorophyll content and photosynthetic efficiency were higher in UV-B -treated leaves left in darkness. UV-B treatment followed by constant light caused leaf yellowing and induced the expression of senescence-related genes. Irrespective of light treatment a high UV-B dose led to clearly visible cell death 3 days after irradiation. CONCLUSIONS: High doses of UV-B have opposing effects on leaves depending on their light status after UV treatment. In darkened leaves short UV-B treatment delays the appearance of senescence symptoms. When followed by light treatment, the same doses of UV-B result in chlorophyll degradation. This restricts the potential usability of UV treatment in postharvest technology to crops which are stored in darkness. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-015-0667-2) contains supplementary material, which is available to authorized users

Physical activity cardio-surgical patients

Health Behaviors are one of the most important factors that determine health. Physical activity plays an important role in the prevention of diseases i.e. hypertension, coronary artery disease, diabetes type 2, stroke and overweight and obesity.In the study this in the clinic of cardiac surgery University Hospital # 1 in Bydgoszcz in the period from October to November 2016 uses the International physical activity questionnaire (IPAQ).  Encouraging patients coronary artery bypass grafting for physical activity before the procedure should be to educate patients about the importance of traffic before the operations and promote health promoting behaviors i.e.. correct diet and maintain a proper body weight, control blood pressure and glucose levels, and appropriate form traffic adapted to the needs and capabilities of the patient

Interaction of ferredoxin:NADP+ oxidoreductase with model membranes

AbstractThe ferredoxin:NADP+ oxidoreductase (FNR) is a plant enzyme, catalyzing the last step of photosynthetic linear electron transport, and involved also in cyclic electron transport around photosystem I. In this study we present the first evidence of FNR (isolated from spinach and from wheat) interaction directly with a model membrane without the mediation of any additional protein. The monomolecular layer technique measurements showed a significant increase in surface pressure after the injection of enzyme solution beneath a monolayer consisting of chloroplast lipids: monogalactosyldiacylglycerol or digalactosyldiacylglycerol. An ATR FTIR study revealed also the presence of FNR in a bilayer composed of these lipids. The secondary structure of the protein was significantly impaired by lipids, as with a pH-induced shift. The stabilization of FNR in the presence of lipids leads to an increase in the rate of NADPH-dependent reduction of dibromothymoquinone catalyzed by the enzyme. The biological significance of FNR–membrane interaction is discussed

Whitener pain - characteristic and fracture

Back pain can be dull dull character, which most often are located only in the lumbar area, in the case where there was not a disagreement radicular -vertebral, or may radiate to the lower limbs, depending on the level and degree of compression of the spinal roots. With a high degree of changes occurring that contribute to the emergence of a significant narrowing of the intervertebral holes and as a result the pressure on the roots, sensory and motor symptoms depend on the level and degree of oppression. It should, however, take into account that even large osteophytes on the anterior-lateral edges of the vertebral bodies, which do not give the roots of oppression, may be clinically silent almost symptomatically. Proper diagnosis, determine the type of pain as well as to diagnose the cause of the pain is important in the further process of rehabilitation

Cadmium inhibitory action leads to changes in structure of ferredoxin:NADP+ oxidoreductase

This study deals with the influence of cadmium on the structure and function of ferredoxin:NADP(+) oxidoreductase (FNR), one of the key photosynthetic enzymes. We describe changes in the secondary and tertiary structure of the enzyme upon the action of metal ions using circular dichroism measurements, Fourier transform infrared spectroscopy and fluorometry, both steady-state and time resolved. The decrease in FNR activity corresponds to a gentle unfolding of the protein, caused mostly by a nonspecific binding of metal ions to multiple sites all over the enzyme molecule. The final inhibition event is most probably related to a bond created between cadmium and cysteine in close proximity to the FNR active center. As a result, the flavin cofactor is released. The cadmium effect is compared to changes related to ionic strength and other ions known to interact with cysteine. The complete molecular mechanism of FNR inhibition by heavy metals is discussed. Electronic supplementary material The online version of this article (doi:10.1007/s10867-012-9262-z) contains supplementary material, which is available to authorized users

Przegląd wybranych metod rehabilitacji kończyny górnej u pacjentów po przebytym udarze niedokrwiennym mózgu = A review of selected methods for the rehabilitation of the upper extremity in patients after undergone brain ischemic stroke

Siminska Joanna, Pietkun Katarzyna, Porzych Piotr, Głowacka Iwona, Świątkowska Anna, Ogurkowski Karol, Grzyb Sebastian, Nowacka Krystyna, Beuth Wojciech. Przegląd wybranych metod rehabilitacji kończyny górnej u pacjentów po przebytym udarze niedokrwiennym mózgu = A review of selected methods for the rehabilitation of the upper extremity in patients after undergone brain ischemic stroke. Journal of Education, Health and Sport. 2016;6(4):240-248. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.50155http://ojs.ukw.edu.pl/index.php/johs/article/view/3466 The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015).755 Journal of Education, Health and Sport eISSN 2391-8306 7© The Author (s) 2016;This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, PolandOpen Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License(http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercialuse, distribution and reproduction in any medium, provided the work is properly cited.The authors declare that there is no conflict of interests regarding the publication of this paper.Received: 20.03.2016. Revised 17.04.2016. Accepted: 17.04.2016.  PRZEGLĄD WYBRANYCH METOD REHABILITACJI KOŃCZYNY GÓRNEJ U PACJENTÓW PO PRZEBYTYM UDARZE NIEDOKRWIENNYM MÓZGUA review of selected methods for the rehabilitation of the upper extremity in patients after undergone brain ischemic stroke Siminska Joanna1, Pietkun Katarzyna1,2, Porzych Piotr1, Głowacka Iwona1, Świątkowska Anna1, Ogurkowski Karol1, Grzyb Sebastian3, Nowacka Krystyna1, Beuth Wojciech4 1Katedra i Klinika Rehabilitacji CM UMK Bydgoszcz2Katedry Kosmetologii i Dermatologii Estetycznej CM UMK Bydgoszcz3Klinika Neurochirurgii, Neurotraumatologii i Neurochirurgii Dziecięcej CM UMK Bydgoszcz4Oddział Neurochirurgii, Wojewódzki Szpital Specjalistyczny, Włocławek Słowa kluczowe: udar niedokrwienny mózgu.Keywords: ischemic stroke.  Streszczenie Analizując dane statystyczne uważa się, że udary mózgu stanowią około 15% wszystkich zgonów na świecie. W Polsce na udar mózgu zapada około 70 tysięcy osób rocznie , spośród których w okresie pierwszego miesiąca umiera około 20%, kolejne 10-25% pacjentów odzyskuje pełną samodzielność, u 40% chorych pozostaje umiarkowana niepełnosprawność, zaś około 15-30%  osób wymaga stałej pomocy osób drugich. Dlatego tak niezwykle istotnym faktem staje się wczesne wdrążenie właściwych algorytmów postępowania procedur rehabilitacji. Wiadomo, że optymalnym okresem powrotu i poprawy funkcji, w tym wykonywania czynności samoobsługowych, są pierwsze 3 miesiące od wystąpienia incydentu udaru mózgu. W tym okresie należy odpowiednio zaplanować i zastosować możliwie najefektywniejsze metody leczenia i usprawniania, co pozwala na poprawę jakości życia jak i podniesienie możliwości wykonywania czynności w zakresie samoobsługi tych chorych. Abstract Analysing statistical data, it is considered that strokes account for approximately 15% of all deaths in the world. In Poland the stroke falls approximately 70 thousand people per year, of which during the first month dies around 20%, 10-25% of patients recover full autonomy in 40% of patients remains a moderate disability, while about 15-30% of people requires constant assistance of another person. Why it is so extremely important fact becomes early implementation of the relevant algorithms conduct rehabilitation procedures. It is known that the optimal period of return and improved features, including self-service operations, are the first 3 months of the occurrence of an incident of stroke. During this period, please plan accordingly and apply as soon as possible the most effective methods of treatment and improve, allowing you to improve the quality of life and raise the possibility of performing self-service operations of these patients

Metody i narzędzia zapisu i udostępniania danych

Praca recenzowana / Peer-reviewed paperPrzekazujemy czytelnikowi kolejną monografię wydaną w ramach serii „Informatyka”, zawierającą publikacje pracowników związanych z Wydziałem Zarządzania i Komunikacji Społecznej Krakowskiej Akademii im. Andrzeja Frycza Modrzewskiego z obszaru problemów optymalizacji, systemów informacyjnych oraz kształcenia zdalnego. Wśród autorów niniejszej publikacji znajdują się głównie pracownicy Studium Informatyki Krakowskiej Akademii i Katedry Informatyki Stosowanej. Opublikowane artykuły są wynikiem współpracy w zespołach z pracownikami innych krakowskich uczelni. Tematyka monografii koncentruje się na metodach optymalizacji, zastosowaniach systemów informatycznych, oraz kształcenia zdalnego. Całość tematyki podzielona jest na trzy wzajemnie powiązane części. Pierwsza część zawiera trzy podrozdziały, w których podjęto kwestie kształcenia zdalnego. Druga część monografii, ujęta w kolejnych trzech podrozdziałach, prezentuje wybrane narzędzia i modele optymalizacji. Ostatnia, trzecia – przedstawia problematykę związaną z zastosowaniem systemów informatycznych w firmie

Comparison of α-Helix and β-Sheet Structure Adaptation to a Quantum Dot Geometry: Toward the Identification of an Optimal Motif for a Protein Nanoparticle Cover

While quantum dots (QDs) are useful as fluorescent labels, their application in biosciences is limited due to the stability and hydrophobicity of their surface. In this study, we tested two types of proteins for use as a cover for spherical QDs, composed of cadmium selenide. Pumilio homology domain (Puf), which is mostly α-helical, and leucine-rich repeat (LRR) domain, which is rich in β-sheets, were selected to determine if there is a preference for one of these secondary structure types for nanoparticle covers. The protein sequences were optimized to improve their interaction with the surface of QDs. The solubilization of the apoproteins and their assembly with nanoparticles required the application of a detergent, which was removed in subsequent steps. Finally, only the Puf-based cover was successful enough as a QD hydrophilic cover. We showed that a single polypeptide dimer of Puf, PufPuf, can form a cover. We characterized the size and fluorescent properties of the obtained QD:protein assemblies. We showed that the secondary structure of the Puf proteins was not destroyed upon contact with the QDs. We demonstrated that these assemblies do not promote the formation of reactive oxygen species during illumination of the nanoparticles. The data represent advances in the effort to obtain a stable biocompatible cover for QDs

Metody analizy i oceny bezpieczeństwa oraz jakości informacji

Praca recenzowana / Peer-reviewed paperTematyka monografii koncentruje się na metodach zapewnienia bezpieczeństwa informacji, procedurach wspomagających podnoszenie jakości danych oraz narzędziach zwiększających możliwości pozyskiwania z dostępnych informacji wartościowych i rzetelnych wniosków analitycznych. Publikacja podzielona jest na trzy części. Pierwsza część zawiera 3 rozdziały, w których podjęto kwestie zapewnienia bezpieczeństwa informacji. Druga część monografii, ujęta w kolejnych trzech rozdziałach, prezentuje wybrane narzędzia i modele organizacji zasobów cyfrowych mających na celu zapewnienie wysokiej jakości, użyteczności oraz wiarygodności zbiorów danych zawartych w repozytoriach. Ostatnia część pracy przedstawia problematykę związaną z prawem Benforda, pozwalającym ocenić stopień rzetelności danych na podstawie analizy rozkładów cyfr w liczbach weryfikowanego zbioru danych