Spectrally selective fluorescence imaging of Chlorobaculum tepidum reaction centers conjugated to chelator-modified silver nanowires

A polyhistidine tag (His-tag) present on Chlorobaculum tepidum reaction centers (RCs) was used to immobilize photosynthetic complexes on a silver nanowire (AgNW) modified with nickel-chelating nitrilo-triacetic acid (Ni-NTA). The optical properties of conjugated nanostructures were studied using wide-field and confocal fluorescence microscopy. Plasmonic enhancement of RCs conjugated to AgNWs was observed as their fluorescence intensity dependence on the excitation wavelength does not follow the excitation spectrum of RC complexes in solution. The strongest effect of plasmonic interactions on the emission intensity of RCs coincides with the absorption spectrum of AgNWs and is observed for excitation into the carotenoid absorption. From the absence of fluorescence decay shortening, we attribute the emission enhancement to increase of absorption in RC complexes

Premises for research on the role of culture in the development of small cities - a case study of Poland

It is widely accepted that cultural sector and creative sector contribute to the socio-economic revival of cities, change their images, form a specific creative milieu, generate new jobs, and organize urban space. However, previous studies concerning the significance of the cultural and creative sectors in the urban development have focused on the largest cities, small cities have not been the subject of a wider research so far. The paper aims at drawing attention to the potential of small cities in the context of using the cultural industry or creatvie industry for the develoment of small cities on the example of Poland. The paper was prepared on the basis of the most relevant literature in the field. The study shows that small cities have a potential that may be used to build development strategies on the basis of the cultural and creative sectors. Research should elaborate the new strategies of development, implement new factors which can initiate required effects in this field

Events as a factor shaping the image of a tourist city

The aim of this paper is to analyze events regarded as elements of the urban image connected with cultural tourism. To achieve this objective, a questionnaire was given to tourists (250 respondents) who visited Toruń during the summer season (June) of 2016. The study established the spatial resonance of the events, demonstrated by the permanent addresses of the tourists who declared that they had heard of particular events. Then the features of the events were assessed in order to check whether those shown to have the greatest reach have a specific profile. The events were ranked according to a multiple-feature point based scale, which confirmed that combination of such elements as high uniqueness of the event, its cohesion with the image of the place, well-established position, broad accessibility and resonance more than local make it possible to achieve optimum marketing effects with regard to the spatial scope of promotional actions. The collected results can be treated as guidelines on the effectiveness of the past actions as well as on the best direction for optimizing such actions in the future

REVITALIZATION AS A PART OF THE EU COHESION POLICY

The aim of the paper is the general characterization of the process of the revitalization in Polish cities in the context of the EU cohesion policy on the example of Kujawsko-Pomorskie voivodship. The research has been conducted on the basis of data derived from the official websites of the Regional Operational Program of Kujawsko-Pomorskie Voivodship 2007-2013 and data obtained from the website of the Ministry of Infrastructure and Development where all projects co-financed from the UE have been currently upgraded and monitored. From database, which contained more than six thousand projects implemented under the Cohesion Policy in Kujawsko-Pomorskie voivodship, 222 projects related to the revitalization have been analyzed. In the paper we have demonstrated that the cohesion policy realized by the EU, has significantly affected the process of the revitalization in Polish cities. This impact can be considered in two ways. On the one hand, the cohesion policy caused the necessity of long-term and integrated planning of all actions required for the activation of problem areas in a city. On the other hand, it initiated instruments by which Polish cities had a real opportunity to start the thorough revitalization

Differences in lifestyle between students of medical and biological fields of study in Poland

Students are a specific social group characterized by different lifestyle behaviours.The aim of the study was to determine whether there are lifestyle differences between students of medical and biological fields from three different universities in Poland.The research material consisted of answers from 1163 students (781 women and 382 men), aged 17.5– 26.0 from the medical faculty of the Wroclaw Medical University, biological faculties of the Cardinal Stefan Wyszynski University in Warsaw and the University of Lodz. The survey included questions regarding gender, socio-demographic situation, lifestyle and eating habits. Students also provided height and weight data, which was used for BMI calculation. Chi-square test and one-way ANOVA were used to indicate differences in BMI between students and to estimate differences in lifestyle between students from three different university centres.Medical students exhibited significantly lower BMI values compared to students from other academic centres. They also more often reported doing additional sports and assessed their overall level of physical activity significantly higher compared to students from other academic centres. Biology students reported to sleep longer and being more exhausted compared to medical students. Biology students tended to drink sugar-sweetened beverages and eat fast-food significantly more often than medical students. Students from the medical faculty in Wroclaw reported to smoke cigarettes less often compared to students from non-medical study. There were no significant differences in other studied factors, such as the use of alcohol, snacking between the meals and consumption of energy drinks.Overall, students of medical fields reported a healthier lifestyle compared to their peers from biological faculties, although this was not consistent for all examined factors

1,8-Naphthalimides 3-substituted with imine or β-ketoenamine unit evaluated as compounds for organic electronics and cell imaging

In this paper, we describe both new as well as described in our previous works 1,8- naphthalimide derivatives substituted at the 3-C position with imine or -ketoenamine unitin order to demonstrate a broader scope of research enabling of analysis between the structureproperties relationship relevant to the application of these compounds in organic electronics and cellular imaging. Thermal, physicochemical, optical, electrochemical, electroluminescence, and biological properties of a series of derivatives containing the 1,8-naphthalimideunit were tested and compared. This allowed the determination of impact of substituents in the imide part (hexylamine, phenylethyl, benzyl, fluorobenzyl, methylbenzyl), type of bond (imine or ketoenamine) as well as the substituent on the naphthalene ring (2-hydroxyphenyl, 5-bromo-2- hydroxyphenyl, 3,5-diodo-2-hydroxyphenyl, pyrimidines) on their properties. Moreover, the properties in the aggregating state were tested in the MeOH/PBS system. Imines are susceptible to the hydrolysis process and aggregation-caused photoluminescence quenching(ACQ). In turn,-ketoenamine shown excited-state intramolecular proton transfer promoted by aggregation (AIEE). Our studies can be helpful in the further design of compounds containing the 1,8- naphthalimide structure for various applications

New acceptor-donor-acceptor systems based on bis-(imino-1,8- naphthalimide)

In this paper, six novel symmetrical bis-(imino-1,8-naphthalimides) differing in core and N-substituent structure were synthesized, and their thermal (TGA, DSC), optical (UV-Vis, PL), electrochemical (DPV, CV) properties were evaluated. The compounds were stable to 280 C and could be transferred into amorphous materials. Electrochemical investigations showed their ability to occur reductions and oxidations processes. They exhibited deep LUMO levels of about -3.22 eV and HOMO levels above -5.80 eV. The optical investigations were carried out in the solutions (polar and non-polar) and in films and blends with PVK:PBD. Bis-(imino-1,8-naphthalimides) absorbed electromagnetic radiation in the range of 243–415 nm and emitted light from blue to yellow. Their capacity for light emission under voltage was preliminarily tested in devices with an active layer consisting of a neat compound and a blend with PVK:PBD. The diodes emitted green or red light

Ground- and excited-state properties of Re(I) carbonyl complexes - effect of triimine ligand core and appended heteroaromatic groups

In this work, a series of six rhenium(I) complexes bearing 2,2′ :6′ ,2′′ -terpyridine (terpy), 2,6-di(thiazol-2-yl)pyridine (dtpy), and 2,6-di(pyrazin-2-yl)pyridine (dppy) with appended quinolin-2-yl and N-ethylcarbazol-3-yl groups were prepared and spectroscopically investigated to evaluate the photophysical consequences of both the trisheterocyclic core (terpy, dtpy and dppy) and the heterocyclic substituent. The [ReCl(CO)3(Ln-κ2N)] complexes are regarded as ideal candidates for getting structure–property relationships, while terpy-like framework represents an excellent structural backbone for structural modifications. The replacement of the peripheral pyridine rings of 2,2′ :6′ ,2′′ -terpyridine by thiazoles and pyrazines resulted in a significant red-shift of the absorption and emission of [ReCl(CO)3(Ln-κ2N)] due to stabilization of the ligand-centred LUMO orbital. Both quinoline and Nethylcarbazole are extended π-conjugation organic chromophores, but they differ in electron-donating abilities. The low-energy absorption band of Re(I) complexes with the triimine ligands bearing quinolin-2-yl group was contributed by the metal-to-ligand charge-transfer (MLCT) electronic transitions. The introduction of electrondonating N-ethylcarbazol-3-yl substituent into the triimine acceptor core resulted in the change of the character of the HOMO of Re(I) complexes and a significant increase of molar absorption coefficients of the longwavelength absorption, which was assigned to a combination of 1MLCT and 1ILCT (intraligand chargetransfer) transitions. Regardless of the appended heteroaromatic group, the emitting excited state of Re(I) terpy-based complexes was demonstrated to have predominant 3MLCT character, as evidenced by comprehensive studies including static and time-resolved emission spectroscopy along with ultrafast transient absorption measurements. The diodes with Re(I) complexes dispersed molecularly in a PVK:PBD matrix were emissive andeffects of the complex structure on colour of emitted light and its intensity was pronounced

Pro Libris: Lubuskie Pismo Literacko-Kulturalne, nr 4 (2010)

145 s. : il., portr.

Value Added Tax in Agriculture in Poland and the European Union. Comparative Analysis

Regulations concerning VAT are of great specificity both in the European Union countries and in Poland. It results, among others, from the character of the agriculture production, its seasonalness as well as related time discrepancy between expenditures and sale receipts of agriculture products. These factors resulted in subjecting the agriculture to the special system of VAT which consists in enabling farmers to account for the tax by means of the flat rate compensation. This regulation is directed mainly to farms of little activity scale. Comparisons revealed that not all of the European Union countries give such opportunity to farmers, what is more, the discrepancy in details of suggested regulations (for example tax rates and lists of products subjected to them) are significant. It results in the fact that VAT burden borne by farmers diverse considerably concerning both the geographic depiction as well as the size and the profile of the farm. The Polish regulations concerning VAT were formulated, so they were adjusted to the 6th Directive regulating the harmonization of indirect taxes within the European Union limits. However, the agriculture was included in the VAT system not earlier than in the year 2000. This way, our law became adjusted to standards of the European Union but, above all, farmers were enabled to regain at least parts of taxes paid on input. At the same time, farmers were given the opportunity to choose between normal and flat rate scheme.Zadanie pt. „Digitalizacja i udostępnienie w Cyfrowym Repozytorium Uniwersytetu Łódzkiego kolekcji czasopism naukowych wydawanych przez Uniwersytet Łódzki” nr 885/P-DUN/2014 zostało dofinansowane ze środków MNiSW w ramach działalności upowszechniającej naukę