34 research outputs found
The Vehicle, Fall 1988
Table of Contents
Cover LetterBob Zordanipage 3
Letter to Harrington StreetBob Zordanipage 4
The Only TruthBob Zordanipage 5
They\u27d Gone to a MovieMatt Mansfieldpage 6
The LocketMonica Grothpage 6
The Sleep of BabesMonica Grothpage 7
Techni-Color Characters in a Black and White TownMonica Grothpage 8
The HorseRodger Patiencepage 9
ValaciaRobyn Kerrpage 10
Gatsby\u27s LightJim Reedpage 11
Millions of MeJim Reedpage 12
View from the StreetsSteven M. Beamerpage 13
When Headlights on the HighwayMichael Salempage 23
Concrete AffairsMichael Salempage 24
The Middle of the StreetMichael Salempage 25
Scent of a StormMichael Salempage 26
The FishermanAngie Geraldpage 27
OrgansPatrick Peterspage 33
CarpentryPatrick Peterspage 34
FishingPatrick Peterspage 35
Autumn Poem for a Friend In a Printing PlantPatrick Peterspage 36https://thekeep.eiu.edu/vehicle/1051/thumbnail.jp
The Vehicle, Fall 1988
Table of Contents
Cover LetterBob Zordanipage 3
Letter to Harrington StreetBob Zordanipage 4
The Only TruthBob Zordanipage 5
They\u27d Gone to a MovieMatt Mansfieldpage 6
The LocketMonica Grothpage 6
The Sleep of BabesMonica Grothpage 7
Techni-Color Characters in a Black and White TownMonica Grothpage 8
The HorseRodger Patiencepage 9
ValaciaRobyn Kerrpage 10
Gatsby\u27s LightJim Reedpage 11
Millions of MeJim Reedpage 12
View from the StreetsSteven M. Beamerpage 13
When Headlights on the HighwayMichael Salempage 23
Concrete AffairsMichael Salempage 24
The Middle of the StreetMichael Salempage 25
Scent of a StormMichael Salempage 26
The FishermanAngie Geraldpage 27
OrgansPatrick Peterspage 33
CarpentryPatrick Peterspage 34
FishingPatrick Peterspage 35
Autumn Poem for a Friend In a Printing PlantPatrick Peterspage 36https://thekeep.eiu.edu/vehicle/1051/thumbnail.jp
The Vehicle, Spring 1989
Table of Contents
Home MoviesBob Zordanipage 4
Mummy BreathMichael Salempage 5
Pop ArtMonica Grothpage 6
Grey Haze and MoonAllison Stroudpage 7
The State of Being at a Soap & SudsDenise Santorpage 9
Letter HomeJim Reedpage 10
Thursday Afternoon in the StacksRebecca Dickenspage 11
Sizing DownMichael Salempage 12
Intellectual AnatomyMonica Grothpage 13
Grandmother PoemAmy Sparkspage 14
Blues of the BrothermanAlma Watsonpage 15
MigrationPatrick Peterspage 17
RidingBob Zordanipage 18
All Hallow\u27s EveErik Hansonpage 19
Waiting RoomAmy Sparkspage 20
Father, Forgive HerMonica Grothpage 21
Silent ReplyTom Caldwellpage 22
PhotographRobb Montgomerypage 24
WashdayAnn Moutraypage 25
PhotographDiane Atkinspage 26
Uptown FogRobb Montgomerypage 27
Shinbones and SkullsJennifer Berkshirepage 29
Sudden Small PhrasesPatrick Peterspage 31https://thekeep.eiu.edu/vehicle/1053/thumbnail.jp
A diverse range of bacterial and eukaryotic chitinases hydrolyzes the LacNAc (Gal<i>β</i>1-4GlcNAc) and LacdiNAc (GalNAc<i>β</i>1-4GlcNAc) motifs found on vertebrate and insect cells
There is emerging evidence that chitinases have additional functions beyond degrading environmental chitin, such as involvement in innate and acquired immune responses, tissue remodeling, fibrosis, and serving as virulence factors of bacterial pathogens. We have recently shown that both the human chitotriosidase and a chitinase from Salmonella enterica serovar Typhimurium hydrolyze LacNAc from Galβ1–4GlcNAcβ-tetramethylrhodamine (LacNAc-TMR (Galβ1–4GlcNAcβ(CH(2))(8)CONH(CH(2))(2)NHCO-TMR)), a fluorescently labeled model substrate for glycans found in mammals. In this study we have examined the binding affinities of the Salmonella chitinase by carbohydrate microarray screening and found that it binds to a range of compounds, including five that contain LacNAc structures. We have further examined the hydrolytic specificity of this enzyme and chitinases from Sodalis glossinidius and Polysphondylium pallidum, which are phylogenetically related to the Salmonella chitinase, as well as unrelated chitinases from Listeria monocytogenes using the fluorescently labeled substrate analogs LacdiNAc-TMR (GalNAcβ1–4GlcNAcβ-TMR), LacNAc-TMR, and LacNAcβ1–6LacNAcβ-TMR. We found that all chitinases examined hydrolyzed LacdiNAc from the TMR aglycone to various degrees, whereas they were less active toward LacNAc-TMR conjugates. LacdiNAc is found in the mammalian glycome and is a common motif in invertebrate glycans. This substrate specificity was evident for chitinases of different phylogenetic origins. Three of the chitinases also hydrolyzed the β1–6 bond in LacNAcβ1–6LacNAcβ-TMR, an activity that is of potential importance in relation to mammalian glycans. The enzymatic affinities for these mammalian-like structures suggest additional functional roles of chitinases beyond chitin hydrolysis
Experimental infection with H1N1 European swine influenza virus protects pigs from an infection with the 2009 pandemic H1N1 human influenza virus
The recent pandemic caused by human influenza virus A(H1N1) 2009 contains ancestral gene segments from North American and Eurasian swine lineages as well as from avian and human influenza lineages. The emergence of this A(H1N1) 2009 poses a potential global threat for human health and the fact that it can infect other species, like pigs, favours a possible encounter with other influenza viruses circulating in swine herds. In Europe, H1N1, H1N2 and H3N2 subtypes of swine influenza virus currently have a high prevalence in commercial farms. To better assess the risk posed by the A(H1N1) 2009 in the actual situation of swine farms, we sought to analyze whether a previous infection with a circulating European avian-like swine A/Swine/Spain/53207/2004 (H1N1) influenza virus (hereafter referred to as SwH1N1) generated or not cross-protective immunity against a subsequent infection with the new human pandemic A/Catalonia/63/2009 (H1N1) influenza virus (hereafter referred to as pH1N1) 21 days apart. Pigs infected only with pH1N1 had mild to moderate pathological findings, consisting on broncho-interstitial pneumonia. However, pigs inoculated with SwH1N1 virus and subsequently infected with pH1N1 had very mild lung lesions, apparently attributed to the remaining lesions caused by SwH1N1 infection. These later pigs also exhibited boosted levels of specific antibodies. Finally, animals firstly infected with SwH1N1 virus and latter infected with pH1N1 exhibited undetectable viral RNA load in nasal swabs and lungs after challenge with pH1N1, indicating a cross-protective effect between both strains
The Vehicle, Fall 1987
Table of Contents
Sketches in the SunRodger L. Patiencepage 3
Reflecting PoolRob Montgomerypage 5
Grandpa\u27s Porcelain DollRichard E. Hallpage 6
Tintype 1837Catherine Friemannpage 6
PhotographSteven M. Beamerpage 7
Washerwoman\u27s SongBob Zordanipage 8
Scrambled Eggs for D.O.Lynne A. Rafoolpage 8
my mother would sayMonica Grothpage 9
Retired by His ChildrenDan Von Holtenpage 10
I am the oldestMonica Grothpage 11
Ice on WheatRob Montgomerypage 12
The Nature of the RoseTroy Mayfieldpage 12
Past NebraskaDan Hornbostelpage 13
Five Minute Jamaican VacationChristy Dunphypage 14
PhotographSteven M. Beamerpage 14
The Angry PoemChristy Dunphypage 15
Road UnfamiliarChristy Dunphypage 15
raised voicesMonica Grothpage 16
Old Ladies & MiniskirtsKara Shannonpage 17
FreakspeakBob Zordanipage 18
PortraitDan Von Holtenpage 18
Mobile VacuumKathleen L. Fairfieldpage 19
Rev. Fermus DickSteve Hagemannpage 20
PhotographSteven M. Beamerpage 21
What\u27s the Name of That Flower?Richard Jesse Davispage 22
RequestChristy Dunphypage 23
SketchPaul Seabaughpage 24
ExperiencedMarilyn Wilsonpage 26
Leaving: Two ViewsTina Phillipspage 27
AntaeusDan Von Holtenpage 28
Misogyny at 19J. D. Finfrockpage 29
A Mental CrippleSteve Hagemannpage 32
AssociationsRhonda Ealypage 33
Banana BreadGail Bowerpage 34
Bill and JackBradford B. Autenpage 35
After Image No. 2Rob Montgomerypage 35
VrrooomBeth Goodmanpage 36
Mr. Modern LoverMolly Maddenpage 36
TravelogueRodger L. Patiencepage 37
Down the HighwayJoan Sebastianpage 38
A Retread HeavenRob Montgomerypage 41
StuporDan Von Holtenpage 42
Love Poem After a Seizure in Your BedBob Zordanipage 43
PalsyChristy Dunphypage 44
Interview with Mr. MatthewsBob Zordanipage 45
Chasing Down Hot Air Balloons on a Sunday MorningRob Montgomerypage 48https://thekeep.eiu.edu/vehicle/1049/thumbnail.jp
Interaction of p21CDKN1A with PCNA regulates the histone acetyltransferase activity of p300 in nucleotide excision repair
The cell-cycle inhibitor p21CDKN1A has been suggested to directly participate in DNA repair, thanks to the interaction with PCNA. Yet, its role has remained unclear. Among proteins interacting with both p21 and PCNA, the histone acetyltransferase (HAT) p300 has been shown to participate in DNA repair. Here we report evidence indicating that p21 protein localizes and interacts with both p300 and PCNA at UV-induced DNA damage sites. The interaction between p300 and PCNA is regulated in vivo by p21. Indeed, loss of p21, or its inability to bind PCNA, results in a prolonged binding to chromatin and an increased association of p300 with PCNA, in UV-irradiated cells. Concomitantly, HAT activity of p300 is reduced after DNA damage. In vitro experiments show that inhibition of p300 HAT activity induced by PCNA is relieved by p21, which disrupts the association between recombinant p300 and PCNA. These results indicate that p21 is required during DNA repair to regulate p300 HAT activity by disrupting its interaction with PCNA
Non-Integrative Lentivirus Drives High-Frequency cre-Mediated Cassette Exchange in Human Cells
Recombinase mediated cassette exchange (RMCE) is a two-step process leading to genetic modification in a specific genomic target sequence. The process involves insertion of a docking genetic cassette in the genome followed by DNA transfer of a second cassette flanked by compatible recombination signals and expression of the recombinase. Major technical drawbacks are cell viability upon transfection, toxicity of the enzyme, and the ability to target efficiently cell types of different origins. To overcome such drawbacks, we developed an RMCE assay that uses an integrase-deficient lentivirus (IDLV) vector in the second step combined with promoterless trapping of double selectable markers. Additionally, recombinase expression is self-limiting as a result of the exchangeable reaction, thus avoiding toxicity. Our approach provides proof-of-principle of a simple and novel strategy with expected wide applicability modelled on a human cell line with randomly integrated copies of a genetic landing pad. This strategy does not present foreseeable limitations for application to other cell systems modified by homologous recombination. Safety, efficiency, and simplicity are the major advantages of our system, which can be applied in low-to-medium throughput strategies for screening of cDNAs, non-coding RNAs during functional genomic studies, and drug screening
Zwitterionically modified alginates mitigate cellular overgrowth for cell encapsulation
Foreign body reaction (FBR) to implanted biomaterials and medical devices is common and can compromise the function of implants or cause complications. For example, in cell encapsulation, cellular overgrowth (CO) and fibrosis around the cellular constructs can reduce the mass transfer of oxygen, nutrients and metabolic wastes, undermining cell function and leading to transplant failure. Therefore, materials that mitigate FBR or CO will have broad applications in biomedicine. Here we report a group of zwitterionic, sulfobetaine (SB) and carboxybetaine (CB) modifications of alginates that reproducibly mitigate the CO of implanted alginate microcapsules in mice, dogs and pigs. Using the modified alginates (SB-alginates), we also demonstrate improved outcome of islet encapsulation in a chemically-induced diabetic mouse model. These zwitterion-modified alginates may contribute to the development of cell encapsulation therapies for type 1 diabetes and other hormone-deficient diseases