Rôle des propriétés physiques et chimiques du milieu dans la succession des protistes marins lors de la floraison printanière en baie de Baffin

Une diminution de l’étendue du couvert de glace et de neige au printemps a été observée en Arctique. Il est attendu que ceci affectera la phénologie des floraisons printanières, de même que la succession des groupes et espèces parmi les producteurs primaires. Les principaux objectifs étaient (i) de caractériser les communautés d’algues de glace et de phytoplancton et (ii) d’identifier les principaux forçages environnementaux associés à la succession des principaux groupes taxonomiques d’algues unicellulaires pendant une floraison printanière en baie de Baffin en 2015 et en 2016. Avec des mesures de variables environnementales à haute résolution temporelle et utilisant un cytomètre en flux imageur submersible (Imaging FlowCytobot) pour l’identification et le décompte des algues (<150 μm), nous avons évalué le rôle de la lumière et de la disponibilité en nutriments dans le contrôle des floraisons printanières d’algues unicellulaires. Les diatomées pennées représentaient principalement les communautés sympagiques. Les communautés phytoplanctoniques étaient initialement semblables à celles observées dans la glace, suggérant un possible ensemencement des floraisons phytoplanctoniques par les algues de glace. Une augmentation de l’intensité lumineuse, principalement causée par la fonte de la neige et l’apparition de cuvettes d’eau de fonte, semble avoir favorisé les diatomées centriques, ces dernières dominant les communautés pélagiques pendant les floraisons phytoplanctoniques des deux années. La disponibilité en lumière semble être le forçage principal limitant le déclenchement des floraisons sympagiques et pélagiques, avec une valeur journalière minimale de 0.1 mol photons m-2 d-1. Une limitation en nutriments dans la glace n’a pas clairement été observée, alors que les nitrates semblent avoir joué un rôle prépondérant dans le déclin de la floraison dans la colonne d’eau. Nos résultats suggèrent qu’il y a un fort potentiel pour des floraisons printanières sous la glace, qui sont actuellement principalement limitées par la lumière tôt dans la saison.With ongoing climate change in the Arctic, a decrease in the extent of sea ice and in the spring snow cover thickness has been observed. A modification of the ice and snow dynamics is predicted to impact the onset, the duration and the decline of microalgae spring blooms, as well as the succession among groups and species of primary producers. The main goals of the present study were (i) to characterize the ice-associated algae and phytoplankton communities and (ii) to identify the main drivers associated with the microalgal main taxonomic groups succession during an under-ice bloom in Baffin Bay in 2015 and 2016. With high-resolution time series of environmental parameters and using an Imaging FlowCytobot for the identification and enumeration of algal cells (<150 μm) within the sea ice bottom and in the underlying water column, we address the role of light and nutrients availability in controlling spring bloom phenology. Pennate diatoms dominated the sympagic community, with different genera dominating for each year. The phytoplankton community was initially alike that found in sea ice, suggesting a possible seeding of the pelagic bloom by the ice algal community. Light availability seemed to be the main factor controlling the onset of both sympagic and pelagic blooms, with a threshold value of 0.1 mol photons m-2 d-1. Through spring, snow and sea ice melting in association with melt pond onset caused the decline of the sympagic bloom, while the increase in under-ice irradiance likely favored centric diatoms, which dominated the protists assemblage during the phytoplankton blooms. Nutrients limitation in sea ice was not observed, while nitrate seemed to play a major role in the decline of the phytoplankton bloom. Our results suggest that there is a potential for early and massive under ice blooms, which are mostly light limited early in the season

Under-ice phytoplankton blooms inhibited by spring convective mixing in refreezing leads

Author Posting. © American Geophysical Union, 2018. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research: Oceans 123 (2018): 90–109, doi:10.1002/2016JC012575.Spring phytoplankton growth in polar marine ecosystems is limited by light availability beneath ice-covered waters, particularly early in the season prior to snowmelt and melt pond formation. Leads of open water increase light transmission to the ice-covered ocean and are sites of air-sea exchange. We explore the role of leads in controlling phytoplankton bloom dynamics within the sea ice zone of the Arctic Ocean. Data are presented from spring measurements in the Chukchi Sea during the Study of Under-ice Blooms In the Chukchi Ecosystem (SUBICE) program in May and June 2014. We observed that fully consolidated sea ice supported modest under-ice blooms, while waters beneath sea ice with leads had significantly lower phytoplankton biomass, despite high nutrient availability. Through an analysis of hydrographic and biological properties, we attribute this counterintuitive finding to springtime convective mixing in refreezing leads of open water. Our results demonstrate that waters beneath loosely consolidated sea ice (84–95% ice concentration) had weak stratification and were frequently mixed below the critical depth (the depth at which depth-integrated production balances depth-integrated respiration). These findings are supported by theoretical model calculations of under-ice light, primary production, and critical depth at varied lead fractions. The model demonstrates that under-ice blooms can form even beneath snow-covered sea ice in the absence of mixing but not in more deeply mixed waters beneath sea ice with refreezing leads. Future estimates of primary production should account for these phytoplankton dynamics in ice-covered waters.National Science Foundation (NSF) Grant Numbers: PLR-1304563 , PLR-1303617; KEL; NSF Graduate Research Fellowship Program Grant Number: DGE-06459622018-07-0

Green Edge ice camp campaigns : understanding the processes controlling the under-ice Arctic phytoplankton spring bloom

The Green Edge initiative was developed to investigate the processes controlling the primary productivity and fate of organic matter produced during the Arctic phytoplankton spring bloom (PSB) and to determine its role in the ecosystem. Two field campaigns were conducted in 2015 and 2016 at an ice camp located on landfast sea ice southeast of Qikiqtarjuaq Island in Baffin Bay (67.4797∘ N, 63.7895∘ W). During both expeditions, a large suite of physical, chemical and biological variables was measured beneath a consolidated sea-ice cover from the surface to the bottom (at 360 m depth) to better understand the factors driving the PSB. Key variables, such as conservative temperature, absolute salinity, radiance, irradiance, nutrient concentrations, chlorophyll a concentration, bacteria, phytoplankton and zooplankton abundance and taxonomy, and carbon stocks and fluxes were routinely measured at the ice camp. Meteorological and snow-relevant variables were also monitored. Here, we present the results of a joint effort to tidy and standardize the collected datasets, which will facilitate their reuse in other Arctic studies

A united statement of the global chiropractic research community against the pseudoscientific claim that chiropractic care boosts immunity.

BACKGROUND: In the midst of the coronavirus pandemic, the International Chiropractors Association (ICA) posted reports claiming that chiropractic care can impact the immune system. These claims clash with recommendations from the World Health Organization and World Federation of Chiropractic. We discuss the scientific validity of the claims made in these ICA reports. MAIN BODY: We reviewed the two reports posted by the ICA on their website on March 20 and March 28, 2020. We explored the method used to develop the claim that chiropractic adjustments impact the immune system and discuss the scientific merit of that claim. We provide a response to the ICA reports and explain why this claim lacks scientific credibility and is dangerous to the public. More than 150 researchers from 11 countries reviewed and endorsed our response. CONCLUSION: In their reports, the ICA provided no valid clinical scientific evidence that chiropractic care can impact the immune system. We call on regulatory authorities and professional leaders to take robust political and regulatory action against those claiming that chiropractic adjustments have a clinical impact on the immune system

Albiglutide and cardiovascular outcomes in patients with type 2 diabetes and cardiovascular disease (Harmony Outcomes): a double-blind, randomised placebo-controlled trial

Background: Glucagon-like peptide 1 receptor agonists differ in chemical structure, duration of action, and in their effects on clinical outcomes. The cardiovascular effects of once-weekly albiglutide in type 2 diabetes are unknown. We aimed to determine the safety and efficacy of albiglutide in preventing cardiovascular death, myocardial infarction, or stroke. Methods: We did a double-blind, randomised, placebo-controlled trial in 610 sites across 28 countries. We randomly assigned patients aged 40 years and older with type 2 diabetes and cardiovascular disease (at a 1:1 ratio) to groups that either received a subcutaneous injection of albiglutide (30–50 mg, based on glycaemic response and tolerability) or of a matched volume of placebo once a week, in addition to their standard care. Investigators used an interactive voice or web response system to obtain treatment assignment, and patients and all study investigators were masked to their treatment allocation. We hypothesised that albiglutide would be non-inferior to placebo for the primary outcome of the first occurrence of cardiovascular death, myocardial infarction, or stroke, which was assessed in the intention-to-treat population. If non-inferiority was confirmed by an upper limit of the 95% CI for a hazard ratio of less than 1·30, closed testing for superiority was prespecified. This study is registered with ClinicalTrials.gov, number NCT02465515. Findings: Patients were screened between July 1, 2015, and Nov 24, 2016. 10 793 patients were screened and 9463 participants were enrolled and randomly assigned to groups: 4731 patients were assigned to receive albiglutide and 4732 patients to receive placebo. On Nov 8, 2017, it was determined that 611 primary endpoints and a median follow-up of at least 1·5 years had accrued, and participants returned for a final visit and discontinuation from study treatment; the last patient visit was on March 12, 2018. These 9463 patients, the intention-to-treat population, were evaluated for a median duration of 1·6 years and were assessed for the primary outcome. The primary composite outcome occurred in 338 (7%) of 4731 patients at an incidence rate of 4·6 events per 100 person-years in the albiglutide group and in 428 (9%) of 4732 patients at an incidence rate of 5·9 events per 100 person-years in the placebo group (hazard ratio 0·78, 95% CI 0·68–0·90), which indicated that albiglutide was superior to placebo (p&lt;0·0001 for non-inferiority; p=0·0006 for superiority). The incidence of acute pancreatitis (ten patients in the albiglutide group and seven patients in the placebo group), pancreatic cancer (six patients in the albiglutide group and five patients in the placebo group), medullary thyroid carcinoma (zero patients in both groups), and other serious adverse events did not differ between the two groups. There were three (&lt;1%) deaths in the placebo group that were assessed by investigators, who were masked to study drug assignment, to be treatment-related and two (&lt;1%) deaths in the albiglutide group. Interpretation: In patients with type 2 diabetes and cardiovascular disease, albiglutide was superior to placebo with respect to major adverse cardiovascular events. Evidence-based glucagon-like peptide 1 receptor agonists should therefore be considered as part of a comprehensive strategy to reduce the risk of cardiovascular events in patients with type 2 diabetes. Funding: GlaxoSmithKline

The Changing Landscape for Stroke\ua0Prevention in AF: Findings From the GLORIA-AF Registry Phase 2

Background GLORIA-AF (Global Registry on Long-Term Oral Antithrombotic Treatment in Patients with Atrial Fibrillation) is a prospective, global registry program describing antithrombotic treatment patterns in patients with newly diagnosed nonvalvular atrial fibrillation at risk of stroke. Phase 2 began when dabigatran, the first non\u2013vitamin K antagonist oral anticoagulant (NOAC), became available. Objectives This study sought to describe phase 2 baseline data and compare these with the pre-NOAC era collected during phase&nbsp;1. Methods During phase 2, 15,641 consenting patients were enrolled (November 2011 to December 2014); 15,092 were eligible. This pre-specified cross-sectional analysis describes eligible patients\u2019 baseline characteristics. Atrial fibrillation&nbsp;disease characteristics, medical outcomes, and concomitant diseases and medications were collected. Data were analyzed using descriptive statistics. Results Of the total patients, 45.5% were female; median age was 71 (interquartile range: 64, 78) years. Patients were from Europe (47.1%), North America (22.5%), Asia (20.3%), Latin America (6.0%), and the Middle East/Africa (4.0%). Most had high stroke risk (CHA2DS2-VASc [Congestive heart failure, Hypertension, Age&nbsp; 6575 years, Diabetes mellitus, previous Stroke, Vascular disease, Age 65 to 74 years, Sex category] score&nbsp; 652; 86.1%); 13.9% had moderate risk (CHA2DS2-VASc&nbsp;= 1). Overall, 79.9% received oral anticoagulants, of whom 47.6% received NOAC and 32.3% vitamin K antagonists (VKA); 12.1% received antiplatelet agents; 7.8% received no antithrombotic treatment. For comparison, the proportion of phase 1 patients (of N&nbsp;= 1,063 all eligible) prescribed VKA was 32.8%, acetylsalicylic acid 41.7%, and no therapy 20.2%. In Europe in phase 2, treatment with NOAC was more common than VKA (52.3% and 37.8%, respectively); 6.0% of patients received antiplatelet treatment; and 3.8% received no antithrombotic treatment. In North America, 52.1%, 26.2%, and 14.0% of patients received NOAC, VKA, and antiplatelet drugs, respectively; 7.5% received no antithrombotic treatment. NOAC use was less common in Asia (27.7%), where 27.5% of patients received VKA, 25.0% antiplatelet drugs, and 19.8% no antithrombotic treatment. Conclusions The baseline data from GLORIA-AF phase 2 demonstrate that in newly diagnosed nonvalvular atrial fibrillation patients, NOAC have been highly adopted into practice, becoming more frequently prescribed than VKA in&nbsp;Europe and North America. Worldwide, however, a large proportion of patients remain undertreated, particularly in&nbsp;Asia&nbsp;and North America. (Global Registry on Long-Term Oral Antithrombotic Treatment in Patients With Atrial Fibrillation [GLORIA-AF]; NCT01468701

Foreperiod and range effects on time interval categorization

One factor influencing the perceived duration of a brief interval is the length of the period preceding it, namely the foreperiod (FP). When multiple FPs are varied randomly within a testing session, longer FPs result in longer perceived duration. The purpose of this study was to identify what characteristics modulate this effect. In a task where participants were asked to categorize the duration of target intervals with respect to a 100-ms standard, the FPs were distributed over a 150-, 300-, or 900-ms range with the midpoint (1000 ms) of these distributions being kept constant. The results indicate that the effect of the length of variable FPs on perceived duration was much stronger in the 900-ms range condition. More specifically, this effect is due to the differences between the shortest FPs. The results also reveal that, overall, there are more short responses in the 300-ms condition than in the other range conditions. Moreover, the data reveal that the narrower the distribution, the better the discrimination. One interpretation of the main result (range effect) is that a wider distribution leads to an increased prior uncertainty towards the foreperiod length. -- Keywords : Perception and action; Attention and memory; Categorization

Comprehensive Mass Spectrometric Metabolomic Profiling of a Chemically Diverse Collection of Plants of the Celastraceae Family

Natural products exhibit interesting structural features and significant biological activities. The discovery of new bioactive molecules is a complex process that requires high-quality metabolite profiling data to properly target the isolation of compounds of interest and enable their complete structural characterization. The same metabolite profiling data can also be used to understand chemotaxonomic links between species better. This Data Descriptor details a dataset resulting from the untargeted liquid chromatography-mass spectrometry profiling of 76 natural extracts of the Celastraceae family. The spectral annotation results and related chemical and taxonomic metadata are shared, along with proposed examples of data reuse. This data can be further studied by researchers exploring the chemical diversity of natural products. This can serve as a reference sample set for deep metabolome investigation of this chemically rich plant family

Comprehensive mass spectrometric metabolomic profiling of a chemically diverse collection of plants of the Celastraceae family

Abstract Natural products exhibit interesting structural features and significant biological activities. The discovery of new bioactive molecules is a complex process that requires high-quality metabolite profiling data to properly target the isolation of compounds of interest and enable their complete structural characterization. The same metabolite profiling data can also be used to better understand chemotaxonomic links between species. This Data Descriptor details a dataset resulting from the untargeted liquid chromatography-mass spectrometry metabolite profiling of 76 natural extracts of the Celastraceae family. The spectral annotation results and related chemical and taxonomic metadata are shared, along with proposed examples of data reuse. This data can be further studied by researchers exploring the chemical diversity of natural products. This can serve as a reference sample set for deep metabolome investigation of this chemically rich plant family