The ICAM-3/LFA-1 interaction is critical for epidermal Langerhans cell alloantigen presentation to CD4 + T cells

Intercellular adhesion molecule (ICAM)-3 is a recently described member of the immunoglobulin superfamily and, as such, is closely related to ICAM-1 and ICAM-2. All three ICAMS are cognate for the counter-receptor lymphocyte function associated antigen-1 (LFA-L CD11a/CD18). Unlike ICAM-1 and ICAM-2. ICAM-3 is constitutively expressed at high levels on resting leucocytes. We investigated the expression and function of ICAM-3 in normal skin ( n = 5), as well as its expression in psoriasis ( n = 4). atopic eczema ( n = 4), allergic (rhus) contact dermatitis ( n =3). and cutaneous T-cell lymphoma (CTCL. n =2). Five-micrometre cryostat sections of skin were stained using monoclonal antibodies to ICAM-3 and A well characterized immunoperoxidase technique. In normal skin. ICAM-3 was expressed by all cutaneous leucocytes hut most striking was the strong expression of ICAM-3 by Langerhans cells within both epidermis and dermis. This observation was confirmed by double-labelling with CD1a and negative staining with an IgG1 isotype control. In psoriasis, atopic eczema, allergic contact dermatitis, and CTCL. ICAM-3 was co-expressed on all CD1a + cells, although, in psoriasis, the intensity of ICAM-3 expression was reduced. Functional blocking experiments were performed to determine whether the observed ICAM-3 expression on Langerhans cells was functionally important in antigen presentation. CD4 + T cells were prepared from peripheral blood and 10 5 CD4 + T cells combined with 10 5 epidermal cells harvested from keratome biopsies of normal skin of an individual allogeneic to the T-cell donor. Addition of 50 Μg anti-ICAM-3 to the co-culture resulted in a consistent (50%) reduction in degree of alloantigen presentation by Langerhans cells to T cells. Inhibition was 77% of that produced by the addition of anti-LFA-1. These data indicate that ICAM-3 is constitutively expressed by Langerhans cells and is a major ligand for LFA-1 on CD4 + T cells during their response to Langerhans cells. Because fresh Langerhans ceils constitutively express little ICAM-1. whereas ICAM-3 is constitutively expressed at high levels, it would appear that 1CAM-3 is the dominant functional ICAM on in situ Langerhans cells in the normal epidermis.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73969/1/j.1365-2133.1995.tb06911.x.pd

Is adolescent body mass index and waist circumference associated with the food environments surrounding schools and homes? A longitudinal analysis

Background: There has been considerable interest in the role of access to unhealthy food options as a determinant of weight status. There is conflict across the literature as to the existence of such an association, partly due to the dominance of cross-sectional study designs and inconsistent definitions of the food environment. The aim of our study is to use longitudinal data to examine if features of the food environment are associated to measures of adolescent weight status. Methods: Data were collected from secondary schools in Leeds (UK) and included measurements at school years 7 (ages 11/12), 9 (13/14), and 11 (15/16). Outcome variables, for weight status, were standardised body mass index and standardised waist circumference. Explanatory variables included the number of fast food outlets, supermarkets and ‘other retail outlets’ located within a 1 km radius of an individual’s home or school, and estimated travel route between these locations (with a 500 m buffer). Multi-level models were fit to analyse the association (adjusted for confounders) between the explanatory and outcome variables. We also examined changes in our outcome variables between each time period. Results: We found few associations between the food environment and measures of adolescent weight status. Where significant associations were detected, they mainly demonstrated a positive association between the number of amenities and weight status (although effect sizes were small). Examining changes in weight status between time periods produced mainly non-significant or inconsistent associations. Conclusions: Our study found little consistent evidence of an association between features of the food environment and adolescent weight status. It suggests that policy efforts focusing on the food environment may have a limited effect at tackling the high prevalence of obesity if not supported by additional strategies

Unveiling pathways for the fissure among secessionists and unionists in Catalonia: identities, family language, and media influence

Catalonian secessionism acquired prominence from 2010 onwards. During the last decade secessionist parties won three regional elections and sustained Governments by tiny majorities at the Autonomous Parliament. Two illegal consultations about self-determination were called and around 2 million (38% of population census) supported secession from Spain. An “Independence Declaration” was proclaimed on 27th Oct. 2017, followed by suspension of Home Rule sanctioned by Spanish Parliament that endured till mid-2018. The main consequence of the secessionist push was the build-up of a confrontation between two large segments of Catalan citizenry, unionists and secessionists, which was absent before. This study aims to shed light on the rise of secessionism and the appearance of a deep fissure between these communities. By building upon the complete series of data from iterated official polls (88.538 respondents, 45 surveys), the paper displays the evolving changes along the period 2006–2019 of national identity feelings (“sense of belonging”). Along that period, there were increases exceeding 15 percentage points of “only Catalan” national identity and analogous decreases of "equally Catalan and Spanish" dual national identity. The findings disclosed highly significant covariations between changing trends on national identity feelings with: (1) family/mother language, Catalan vs. Spanish; (2) following regional media versus other media. Since language/ascendancy origins and media consumption trends are closely interrelated, within Catalonia, our discussion focuses on the role played by such ethno-cultural cleavage. Further, statistical analysis for longitudinal data identified several turning points linked to singular political events that likely accentuated polarization around the issue of secession. The findings unveil evolving tracks that could help in the understanding of a process that, in a very short time, produced a severe social division within a fully open and democratic society at the heart of Europe.Damián Gil M.D. provided the first script that permitted to build the database of the full series of CEO Barometers. OEC Group members provided lively discussions with suggestions that improved and helped to focus the present research. A.T. work was partially supported by AFOSR-MINERVA FA9550-18-0496 Grant and Bial Foundation Grant 163/14.Damián Gil M.D. provided the first script that permitted to build the database of the full series of CEO Barometers. OEC Group members provided lively discussions with suggestions that improved and helped to focus the present research. A.T. work was partially supported by AFOSR-MINERVA FA9550-18-0496 Grant and Bial Foundation Grant 163/14

Location and the multinational enterprise: John Dunning's thoughts on receiving the Journal of International Business Studies 2008 Decade Award

A retrospective by John H. Dunning on location and the MNE, written in the form of responses to questions posed by Rajneesh Narula in an interview recorded at the University of Reading which was shown at the JIBS Decade Award session at the AIB annual meetings in Milan, June 2008. Journal of International Business Studies (2009) 40, 20–34. doi:10.1057/jibs.2008.75