3 research outputs found
Towards nanomedicines of the future: Remote magneto-mechanical actuation of nanomedicines by alternating magnetic fields
The paper describes the concept of magneto-mechanical actuation of single-domain magnetic nanoparticles (MNPs) in super-low and low frequency alternating magnetic fields (AMFs) and its possible use for remote control of nanomedicines and drug delivery systems. The applications of this approach for remote actuation of drug release as well as effects on biomacromolecules, biomembranes, subcellular structures and cells are discussed in comparison to conventional strategies employing magnetic hyperthermia in a radio frequency (RF) AMF. Several quantitative models describing interaction of functionalized MNPs with single macromolecules, lipid membranes, and proteins (e.g. cell membrane receptors, ion channels) are presented. The optimal characteristics of the MNPs and an AMF for effective magneto-mechanical actuation of single molecule responses in biological and bio-inspired systems are discussed. Altogether, the described studies and phenomena offer opportunities for the development of novel therapeutics both alone and in combination with magnetic hyperthermia
<i>In Situ</i> Observation of Chymotrypsin Catalytic Activity Change Actuated by Nonheating Low-Frequency Magnetic Field
Magnetomechanical
modulation of biochemical processes is a promising
instrument for bioengineering and nanomedicine. This work demonstrates
two approaches to control activity of an enzyme, α-chymotrypsin
immobilized on the surface of gold-coated magnetite magnetic nanoparticles
(GM-MNPs) using a nonheating low-frequency magnetic field (LF MF).
The measurement of the enzyme reaction rate was carried out <i>in situ</i> during exposure to the magnetic field. The first
approach involves α-chymotrypsin-GM-MNPs conjugates, in which
the enzyme undergoes mechanical deformations with the reorientation
of the MNPs under LF MF (16–410 Hz frequency, 88 mT flux density).
Such mechanical deformations result in conformational changes in α-chymotrypsin
structure, as confirmed by infrared spectroscopy and molecular modeling,
and lead to a 63% decrease of enzyme initial activity. The second
approach involves an α-chymotrypsin–GM-MNPs/trypsin inhibitor–GM-MNPs
complex, in which the activity of the enzyme is partially inhibited.
In this case the reorientation of MNPs in the field leads to disruption
of the enzyme–inhibitor complex and an almost 2-fold increase
of enzyme activity. The results further demonstrate the utility of
magnetomechanical actuation at the nanoscale for the remote modulation
of biochemical reactions
Towards nanomedicines of the future: Remote magneto-mechanical actuation of nanomedicines by alternating magnetic fields
The paper describes the concept of magneto-mechanical actuation of single-domain magnetic nanoparticles (MNPs) in super-low and low frequency alternating magnetic fields (AMFs) and its possible use for remote control of nanomedicines and drug delivery systems. The applications of this approach for remote actuation of drug release as well as effects on biomacromolecules, biomembranes, subcellular structures and cells are discussed in comparison to conventional strategies employing magnetic hyperthermia in a radio frequency (RF) AMF. Several quantitative models describing interaction of functionalized MNPs with single macromolecules, lipid membranes, and proteins (e.g. cell membrane receptors, ion channels) are presented. The optimal characteristics of the MNPs and an AMF for effective magneto-mechanical actuation of single molecule responses in biological and bio-inspired systems are discussed. Altogether, the described studies and phenomena offer opportunities for the development of novel therapeutics both alone and in combination with magnetic hyperthermia