Evaluating essential processes and forecast requirements for meteotsunami-induced coastal flooding

Meteotsunamis pose a unique threat to coastal communities and often lead to damage of coastal infrastructure, deluge of nearby property, and loss of life and injury. The Great Lakes are a known hot-spot of meteotsunami activity and serve as an important region for investigation of essential hydrodynamic processes and model forecast requirements in meteotsunami-induced coastal flooding. For this work, we developed an advanced hydrodynamic model and evaluate key model attributes and dynamic processes, including: (1) coastal model grid resolution and wetting and drying process in low-lying zones, (2) coastal infrastructure, including breakwaters and associated submerging and overtopping processes, (3) annual/seasonal (ambient) water level change, and (4) wind wave-current coupling. Numerical experiments are designed to evaluate the importance of these attributes to meteotsunami modeling, including a “representative storm” scenario in the context of regional climate change in which a meteotsunami wave is generated under high ambient lake-level conditions with a preferable wind direction and speed for wind-wave growth. Results demonstrate that accurate representation of coastal topography and fully resolving associated hydrodynamic processes are critical to forecasting the realistic hazards associated with meteotsunami events. As most of existing coastal forecast systems generally do not resolve many of these features due to insufficient model grid resolution or lack of essential model attributes, this work shows that calibrating or assessing existing forecast models against coastal water level gauges alone may result in underestimating the meteotsunami hazard, particularly when gauging stations are sparse and located behind harbor breakwaters or inside estuaries, which represent dampened or otherwise unrepresentative pictures of meteotsunami intensity. This work is the first hydrodynamic modeling of meteotsunami-induced coastal flooding for the Great Lakes, and serves as a template to guide where resources may be most beneficial in forecast system development and implementation

Increasing seedstock production of domesticated giant tiger prawns

Given the rationale that pond systems are likely the most cost-effective system for large-scale production of P. monodon broodstock, this project aimed to determine whether pond-rearing poses a significant risk for broodstock production. The gross reproductive development of males reared in low-density broodstock ponds was found comparable to sibling males reared in controlled-environment tanks. Furthermore,none of the environmental ‘stressors’ and dietary manipulations examined impacted on male reproductive tract development. Thus, within the boundaries of the parameters tested, we can state that rearing of male broodstock in low-density ponds does not pose inherent risks of gross reproductive tract impairment. Given the typically lower costs of constructing and managing broodstock in large-scale pond systems, as compared to smaller raceway and tank systems, the incorporation of a pond-rearing phase in broodstock production could clearly increase cost-effectiveness of broodstock production at a commercial scale

Repo-Man recruits PP1γ to chromatin and is essential for cell viability

Protein phosphatase 1 (PP1) is a ubiquitous serine/threonine phosphatase regulating many cellular processes. PP1α and -γ are closely related isoforms with distinct localization patterns, shown here by time-lapse microscopy of stably expressed fluorescent protein fusions. A pool of PP1γ is selectively loaded onto chromatin at anaphase. Using stable isotope labeling and proteomics, we identified a novel PP1 binding protein, Repo-Man, which selectively recruits PP1γ onto mitotic chromatin at anaphase and into the following interphase. This approach revealed both novel and known PP1 binding proteins, quantitating their relative distribution between PP1α and -γ in vivo. When overexpressed, Repo-Man can also recruit PP1α to chromatin. Mutating Repo-Man's PP1 binding domain does not disrupt chromatin binding but abolishes recruitment of PP1 onto chromatin. RNA interference–induced knockdown of Repo-Man caused large-scale cell death by apoptosis, as did overexpression of this dominant-negative mutant. The data indicate that Repo-Man forms an essential complex with PP1γ and is required for the recruitment of PP1 to chromatin

Report on the State of Available Data for the Study of International Trade and Foreign Direct Investment

This report, prepared for the Committee on Economic Statistics of the American Economic Association, examines the state of available data for the study of international trade and foreign direct investment. Data on values of imports and exports of goods are of high quality and coverage, but price data suffer from insufficient detail. It would be desirable to have more data measuring value-added in trade as well as prices of comparable domestic and imported inputs. Value data for imports and exports of services are too aggregated and valuations are questionable, while price data for service exports and imports are almost non-existent. Foreign direct investment data are of high quality but quality has suffered from budget cuts. Data on trade in intellectual property are fragmentary. The intangibility of the trade makes measurement difficult, but budget cuts have added to the difficulties. Modest funding increases would result in data more useful for research and policy analysis.

The structural biology of patellamide biosynthesis

This work was supported by grants from the ERC339367 (JHN and MJ) and BBSRCBB/K015508/1 (JHN and MJ).The biosynthetic pathways for patellamide and related natural products have recently been studied by structural biology. These pathways produce molecules that have a complex framework and exhibit a diverse array of activity due to the variability of the amino acids that are found in them. As these molecules are difficult to synthesize chemically, exploitation of their properties has been modest. The patellamide pathway involves amino acid heterocyclization, peptide cleavage, peptide macrocyclization, heterocycle oxidation and epimerization; closely related products are also prenylated. Enzyme activities have been identified for all these transformations except epimerization, which may be spontaneous. This review highlights the recent structural and mechanistic work on amino acid heterocyclization, peptide cleavage and peptide macrocyclization. This work should help in using the enzymes to produce novel analogs of the natural products enabling an exploitation of their properties.Peer reviewe

Structure and substrate recognition of the Bottromycin maturation enzyme BotP

JK would like to thank the University of St Andrews, which is supported by a Wellcome Trust Capital Award (086036) and the Deutsche Forschungsgemeinschaft for an Emmy Noether fellowship (KO4116/3-1). BN would like to thank the European Research Council (339367).The bottromycins are a family of highly modified peptide natural products displaying potent antimicrobial activity against Gram-positive bacteria including methicillin-resistant Staphyloccoccus aureus. Bottromycins have recently been shown to be ribosomally synthesized and post-translationally modified peptides (RiPPs). Unique amongst RiPPs the precursor peptide BotA contains a C-terminal "follower" sequence, rather than the canonical N- terminal "leader" sequence. We report the structural and biochemical characterization of BotP, a leucyl-aminopeptidase like enzyme from the bottromycin pathway. We demonstrate that BotP is responsible for the removal of the N-terminal methionine from the precursor peptide. Determining the crystal structures of apo BotP and of BotP in complex with Mn2+ allowed us to model a BotP/substrate complex and to rationalize substrate recognition. Our data represent the first step towards targeted compound modification to unlock the full antibiotic potential of bottromycin.PostprintPeer reviewe

Reconstruction of a meteotsunami in Lake Erie on 27 May 2012: Roles of atmospheric conditions on hydrodynamic response in enclosed basins

On 27 May 2012, atmospheric conditions gave rise to two convective systems that generated a series of waves in the meteotsunami band on Lake Erie. The resulting waves swept three swimmers a 0.5 mi offshore, inundated a marina, and may have led to a capsized boat along the southern shoreline. Analysis of radial velocities from a nearby radar tower in combination with coastal meteorological observation indicates that the convective systems produced a series of outflow bands that were the likely atmospheric cause of the meteotsunami. In order to explain the processes that led to meteotsunami generation, we model the hydrodynamic response to three meteorological forcing scenarios: (i) the reconstructed atmospheric disturbance from radar analysis, (ii) simulated conditions from a high‐resolution weather model, and (iii) interpolated meteorological conditions from the NOAA Great Lakes Coastal Forecasting System. The results reveal that the convective systems generated a series of waves incident to the southern shore of the lake that reflected toward the northern shoreline and reflected again to the southern shore, resulting in spatial wave focusing and edge wave formation that combined to impact recreational users near Cleveland, OH. This study illustrates the effects of meteotsunami development in an enclosed basin, including wave reflection, focusing, and edge wave formation as well as temporal lags between the causative atmospheric conditions and arrival of dangerous wave conditions. As a result, the ability to detect these extreme storms and predict the hydrodynamic response is crucial to reducing risk and building resilient coastal communities.Key Points:Radar‐based velocity allows for detection of meteotsunami‐inducing frontWave reflection, focusing, and edge waves led to meteotsunami conditionsEnclosed basins can induce temporal lags between storm front and wave responsePeer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/145384/1/jgrc21485_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/145384/2/jgrc21485-sup-0001-2015JC010883-s01.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/145384/3/jgrc21485.pd

Structure of the cyanobactin oxidase ThcOx from Cyanothece sp. PCC 7425, the first structure to be solved at Diamond Light Source beamline I23 by means of S-SAD

Acknowledgements We thank Diamond Light Source for providing access to beamline I23. We also thank Thomas Sorensen and his staff for access to and support on beamline I02. This research was supported by grants from the UK Biotechnology and Biological Research Council (No. BB/K015508/1; JHN and MJ) and the European Research Council (No. 339367; JHN and MJ). Mass-spectrometric analysis was carried out by the Biomedical Sciences Research Complex Mass Spectrometry and Proteomics Facility, University of St Andrews and was funded by the Wellcome Trust (grant Nos. 094476/Z/10/Z and WT079272AIA). JHN is a Royal Society Wolfson Merit Award Holder and 1000 Talent scholar at Sichuan University.Peer reviewedPublisher PD

Ground-Based Submillimagnitude CCD Photometry of Bright Stars Using Snapshot Observations

We demonstrate ground-based submillimagnitude (<10^-3) photometry of widely separated bright stars using snapshot CCD imaging. We routinely achieved this photometric precision by (1) choosing nearby comparison stars of a similar magnitude and spectral type, (2) defocusing the telescope to allow high signal (>10^7 electrons) to be acquired in a single integration, (3) pointing the telescope so that all stellar images fall on the same detector pixels, and (4) using a region of the CCD detector that is free of nonlinear or aberrant pixels. We describe semiautomated observations with the Supernova Integrated Field Spectrograph (SNIFS) on the University of Hawaii 2.2m telescope on Mauna Kea, with which we achieved photometric precision as good as 5.2x10^-4 (0.56mmag) with a 5 minute cadence over a 2hr interval. In one experiment, we monitored eight stars, each separated by several degrees, and achieved submillimagnitude precision with a cadence (per star) of ~17 minutes. Our snapshot technique is suitable for automated searches for planetary transits among multiple bright stars.Comment: Accepted to PAS

Last millennium northern hemisphere summer temperatures from tree rings: Part I: The long term context

Large-scale millennial length Northern Hemisphere (NH) temperature reconstructions have been progressively improved over the last 20 years as new datasets have been developed. This paper, and its companion (Part II, Anchukaitis et al. in prep), details the latest tree-ring (TR) based NH land air temperature reconstruction from a temporal and spatial perspective. This work is the first product of a consortium called N-TREND (Northern Hemisphere Tree-Ring Network Development) which brings together dendroclimatologists to identify a collective strategy for improving large-scale summer temperature reconstructions. The new reconstruction, N-TREND2015, utilises 54 records, a significant expansion compared with previous TR studies, and yields an improved reconstruction with stronger statistical calibration metrics. N-TREND2015 is relatively insensitive to the compositing method and spatial weighting used and validation metrics indicate that the new record portrays reasonable coherence with large scale summer temperatures and is robust at all time-scales from 918 to 2004 where at least 3 TR records exist from each major continental mass. N-TREND2015 indicates a longer and warmer medieval period (∼900–1170) than portrayed by previous TR NH reconstructions and by the CMIP5 model ensemble, but with better overall agreement between records for the last 600 years. Future dendroclimatic projects should focus on developing new long records from data-sparse regions such as North America and eastern Eurasia as well as ensuring the measurement of parameters related to latewood density to complement ring-width records which can improve local based calibration substantially