56 research outputs found
The utilization of seawater for the hydrolysis of macroalgae and subsequent bioethanol fermentation
The application of phenotypic microarray analysis to anti-fungal drug development
Candida albicans metabolic activity in the presence and absence of acetylcholine was measured using phenotypic microarray analysis. Acetylcholine inhibited C. albicans biofilm formation by slowing metabolism independent of biofilm forming capabilities. Phenotypic microarray analysis can therefore be used for screening compound libraries for novel anti-fungal drugs and measuring antifungal resistance
Tolerance of pentose utilising yeast to hydrogen peroxide-induced oxidative stress.
BACKGROUND: Bioethanol fermentations follow traditional beverage fermentations where the yeast is exposed to adverse conditions such as oxidative stress. Lignocellulosic bioethanol fermentations involve the conversion of pentose and hexose sugars into ethanol. Environmental stress conditions such as osmotic stress and ethanol stress may affect the fermentation performance; however, oxidative stress as a consequence of metabolic output can also occur. However, the effect of oxidative stress on yeast with pentose utilising capabilities has yet to be investigated. RESULTS: Assaying for the effect of hydrogen peroxide-induced oxidative stress on Candida, Pichia and Scheffersomyces spp. has demonstrated that these yeast tolerate hydrogen peroxide-induced oxidative stress in a manner consistent with that demonstrated by Saccharomyces cerevisiae. Pichia guillermondii appears to be more tolerant to hydrogen peroxide-induced oxidative stress when compared to Candida shehatae, Candida succiphila or Scheffersomyces stipitis. CONCLUSIONS: Sensitivity to hydrogen peroxide-induced oxidative stress increased in the presence of minimal media; however, addition of amino acids and nucleobases was observed to increase tolerance. In particular adenine increased tolerance and methionine reduced tolerance to hydrogen peroxide-induced oxidative stress
The development of a biorefining strategy for the production of biofuel from sorghum milling waste
Exploring the tolerance of marine yeast to inhibitory compounds for improving bioethanol production
Impact of mixed feedstock of wheat straw, willow and Miscanthus on enzymatic hydrolysis and inhibitor production after microwave hydrothermal pre-treatment in Europe
Lignocellulosic feedstocks for biorefinery are likely to be seasonal and the supply of feedstock to cellulosic biorefineries remains a challenge. One way to overcome this is by utilizing a mixed feedstock which facilitates the maintenance of a year-round feedstock supply. This study investigated the impact of mixing of three industrially relevant cellulosic feedstocks—wheat straw, willow and Miscanthus using two major performance indicators—sugar yield and fermentation inhibitor production. A microwave hydrothermal pre-treatment regime of 200 °C for 5 min was applied to each feedstock individually and to 1:1 (w/w) mixes and the predicted sugar yield in the mixes was compared to the observed values. All the mixes resulted in improved sugar yields with willow + Miscanthus (15.4%, p = 0.015) and wheat + willow (13.6%, p = 0.010) showing a statistically significant improvement. Saccharification kinetics, inhibitor production, impact on yeast metabolic activity and growth were compared and no adverse impacts of mixing were observed. The use of mixed feedstocks in a hot water based commercial production of biofuels is unlikely to have any adverse effects on productivity and may indeed prove beneficial
Systematic analysis of the DNA damage response network in telomere defective budding yeast
Functional telomeres are critically important to eukaryotic genetic stability. Scores of proteins and pathways are known to affect telomere function. Here, we report a series of related genome-wide genetic interaction screens performed on budding yeast cells with acute or chronic telomere defects. Genetic interactions were examined in cells defective in Cdc13 and Stn1, affecting two components of CST, a single stranded DNA (ssDNA) binding complex that binds telomeric DNA. For comparison, genetic interactions were also examined in cells with defects in Rfa3, affecting the major ssDNA binding protein, RPA, which has overlapping functions with CST at telomeres. In more complex experiments, genetic interactions were measured in cells lacking EXO1 or RAD9, affecting different aspects of the DNA damage response, and containing a cdc13-1 induced telomere defect. Comparing fitness profiles across these data sets helps build a picture of the specific responses to different types of dysfunctional telomeres. The experiments show that each context reveals different genetic interactions, consistent with the idea that each genetic defect causes distinct molecular defects. To help others engage with the large volumes of data, the data are made available via two interactive web-based tools: Profilyzer and DIXY. One particularly striking genetic interaction observed was that the chk1∆ mutation improved fitness of cdc13-1 exo1∆ cells more than other checkpoint mutations (ddc1∆, rad9∆, rad17∆, and rad24∆), whereas, in cdc13-1 cells, the effects of all checkpoint mutations were similar. We show that this can be explained by Chk1 stimulating resection—a new function for Chk1 in the eukaryotic DNA damage response network
Effect of operating conditions on molasses fermentation for bioethanol production
The aim of the presented study was to evaluate the potential of molasses as bioethanol feedstock by studying the effect of different operating conditions on fermentation yield including initial sugar concentration (25-150 g/L), pH (4.5-9.5), and temperature (30-50°C). Molasses composition analyses indicated its richness with sucrose and fermentable sugars which qualify it as a promising feedstock for bioethanol production. The highest ethanol production (49 g/L) was achieved with an initial sugar = 150 g/L, pH = 4.5 but the maximum ethanol yield has been noted for: initial sugar concentration = 50 g/L, pH=4.5 and 30 °C of temperature which represent the optimum conditions for the fermentation. The kinetics study of fermentation experiment carried out under optimal conditions revealed that the fermentation reaction occurs in 3 phases: lag phase, acceleration phase and final phase
Thioredoxins function as deglutathionylase enzymes in the yeast Saccharomyces cerevisiae
<p>Abstract</p> <p>Background</p> <p>Protein-SH groups are amongst the most easily oxidized residues in proteins, but irreversible oxidation can be prevented by protein glutathionylation, in which protein-SH groups form mixed disulphides with glutathione. Glutaredoxins and thioredoxins are key oxidoreductases which have been implicated in regulating glutathionylation/deglutathionylation in diverse organisms. Glutaredoxins have been proposed to be the predominant deglutathionylase enzymes in many plant and mammalian species, whereas, thioredoxins have generally been thought to be relatively inefficient in deglutathionylation.</p> <p>Results</p> <p>We show here that the levels of glutathionylated proteins in yeast are regulated in parallel with the growth cycle, and are maximal during stationary phase growth. This increase in glutathionylation is not a response to increased reactive oxygen species generated from the shift to respiratory metabolism, but appears to be a general response to starvation conditions. Our data indicate that glutathionylation levels are constitutively high in all growth phases in thioredoxin mutants and are unaffected in glutaredoxin mutants. We have confirmed that thioredoxins, but not glutaredoxins, catalyse deglutathionylation of model glutathionylated substrates using purified thioredoxin and glutaredoxin proteins. Furthermore, we show that the deglutathionylase activity of thioredoxins is required to reduce the high levels of glutathionylation in stationary phase cells, which occurs as cells exit stationary phase and resume vegetative growth.</p> <p>Conclusions</p> <p>There is increasing evidence that the thioredoxin and glutathione redox systems have overlapping functions and these present data indicate that the thioredoxin system plays a key role in regulating the modification of proteins by the glutathione system.</p
Screening of Non- Saccharomyces cerevisiae Strains for Tolerance to Formic Acid in Bioethanol Fermentation.
Formic acid is one of the major inhibitory compounds present in hydrolysates derived from lignocellulosic materials, the presence of which can significantly hamper the efficiency of converting available sugars into bioethanol. This study investigated the potential for screening formic acid tolerance in non-Saccharomyces cerevisiae yeast strains, which could be used for the development of advanced generation bioethanol processes. Spot plate and phenotypic microarray methods were used to screen the formic acid tolerance of 7 non-Saccharomyces cerevisiae yeasts. S. kudriavzeii IFO1802 and S. arboricolus 2.3319 displayed a higher formic acid tolerance when compared to other strains in the study. Strain S. arboricolus 2.3319 was selected for further investigation due to its genetic variability among the Saccharomyces species as related to Saccharomyces cerevisiae and availability of two sibling strains: S. arboricolus 2.3317 and 2.3318 in the lab. The tolerance of S. arboricolus strains (2.3317, 2.3318 and 2.3319) to formic acid was further investigated by lab-scale fermentation analysis, and compared with S. cerevisiae NCYC2592. S. arboricolus 2.3319 demonstrated improved formic acid tolerance and a similar bioethanol synthesis capacity to S. cerevisiae NCYC2592, while S. arboricolus 2.3317 and 2.3318 exhibited an overall inferior performance. Metabolite analysis indicated that S. arboricolus strain 2.3319 accumulated comparatively high concentrations of glycerol and glycogen, which may have contributed to its ability to tolerate high levels of formic acid
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