Epidermal growth factor-mediated T-cell factor/lymphoid enhancer factor transcriptional activity is essential but not sufficient for cell cycle progression in nontransformed mammary epithelial cells

Because beta-catenin target genes such as cyclin D1 are involved in cell cycle progression, we examined whether beta-catenin has a more pervasive role in normal cell proliferation, even upon stimulation by non-Wnt ligands. Here, we demonstrate that epidermal growth factor (EGF) stimulates T-cell factor/lymphoid enhancer factor (Tcf/Lef) transcriptional activity in nontransformed mammary epithelial cells (MCF-10A) and that its transcriptional activity is essential for EGF-mediated progression through G(1)/S phase. Thus, expression of dominant-negative Tcf4 blocks EGF-mediated Tcf/Lef transcriptional activity and bromodeoxyuridine uptake. In fact, the importance of EGF-mediated Tcf/Lef transcriptional activity for cell cycle progression may lie further upstream at the G(1)/S phase transition. We demonstrate that dominant-negative Tcf4 inhibits a reporter of cyclin D1 promoter activity in a dose-dependent manner. Importantly, dominant-negative Tcf4 suppresses EGF- mediated cell cycle activity specifically by thwarting EGF- mediated Tcf/Lef transcriptional activity, not by broader effects on EGF signaling. Thus, although expression of dominant-negative Tcf4 blocks EGF- mediated TOPFLASH activation, it has no effect on either EGF receptor or ERK phosphorylation, further underscoring the fact that Tcf/ Lef-mediated transcription is essential for cell cycle progression, even when other pro-mitogenic signals are at normal levels. Yet, despite its essential role, Tcf/Lef transcriptional activity alone is not sufficient for cell cycle progression. Serum also stimulates Tcf/ Lef transcriptional activation in MCF-10A cells but is unable to promote DNA synthesis. Taken together, our data support a model wherein EGF promotes Tcf/ Lef transcriptional activity, and this signal is essential but not sufficient for cell cycle activity

Tracing the Dynamics of Disk Galaxies with Optical and IR Surface Photometry: Color Gradients in M99

We present optical and IR surface photometry of M99 (NGC 4254) at g, r_S i, J and K'. We also present a K' image of M51 (NGC 5194) for comparison. Fourier decomposition of the disk light reveals that the radial distribution of power depends on wavelength, which in turn implies that the spiral structure traced in the visual (i.e. young population I and dust) is different from the one detected at 2 microns (i.e. old stellar disk). We observe radial modulation of the power and a dependency of power with wavelength that are consistent with modal theory of spiral structure. A central motivation for our research is the fundamental idea of density wave theory that the passage of a spiral density wave triggers star formation. We have found a stellar population age gradient consistent with this scenario in a reddening-free, red supergiant-sensitive, Q-like photometric parameter at 6 kpc galactocentric distance across one of the arms of M99. We rule out that the change in this parameter, Q(r_SJgi), across the arm is mainly due to dust. The difference in Q(r_SJgi) going from the interarm regions to the arms also indicates that arms cannot be due exclusively to crowding of stellar orbits. We present the first measurement of Omega_p, the angular speed of the spiral pattern, and of the location of the corotation radius, derived from the drift velocity of the young stars away from their birth site. The measured Q(r_SJgi) implies a star formation rate for M99 within the range of 10-20 M_odot/yr; a disk stellar mass surface density of ~80 M_odot/pc^2; and a maximum contribution of ~20 percent from red supergiants to the K' light in a small region, and much smaller on average. We measure a K' arm--interarm contrast of 2-3, too high for M99 to be a truly isolated galaxy.Comment: 25 pages of uuencoded, compressed Postscript (text only). To appear in 1 April 1996 issue of The Astrophysical Journal. Also available, together with 2 uuencoded, compressed PostScript files with 10 figures each, at http://astro.berkeley.edu/preprints.htm

A review of NASA combustor and turbine heat transfer research

The thermal design of the combustor and turbine of a gas turbine engine poses a number of difficult heat transfer problems. The importance of improved prediction techniques becomes more critical in anticipation of future generations of gas turbine engines which will operate at higher cycle pressure and temperatures. Research which addresses many of the complex heat transfer processes holds promise for yielding significant improvements in prediction of metal temperatures. Such research involves several kinds of program including: (1) basic experiments which delineate the fundamental flow and heat transfer phenomena that occur in the hot sections of the gas turbine but at low enthalpy conditions; (2) analytical modeling of these flow and heat transfer phenomena which results from the physical insights gained in experimental research; and (3) verification of advanced prediction techniques in facilities which operate near the real engine thermodynamic conditions. In this paper, key elements of the NASA program which involves turbine and combustor heat transfer research will be described and discussed

A contribution to the discussion on the safety of air weapons

Firearms legislation in the UK stems from the Firearms Act 1968 with its definition of a firearm as a lethal barrelled weapon of any description. The Act allows certain exceptions to be held without licence, most notably air weapons although these are limited by The Firearms (Dangerous Air Weapons) Rules 1969 and related regulations to below 12 ft lb (16.3 J) for air rifles and below 6 ft lb (8.1 J) for air pistols. Despite this there are occasional fatalities, typically 1 or 2 each year in the UK, from legally owned air weapons. In the USA there are over 20,000 visits each year to emergency departments due to injuries from air weapons and paintball guns. Despite this, limited research appears to have been carried out into the safety of air weapons and the present study tries to address this.Fresh samples of animal tissue were obtained from an abattoir or butcher and were embedded in ballistic gelatin. Pig heart, lung, liver and shoulder were used. By firing pellets into gelatin alone and into the combination of the gelatin and animal tissue it was possible to compare gelatin as a model for these tissues. The depth of penetration was similar but the residual track appeared to remain more open in the animal tissue. Pellets penetrated completely through the organ, with total penetration of gelatin and organ being typically around 10–15 cm.Samples of pig, cow and chicken skin were placed in contact with the gelatin or embedded in the gelatin to simulate the effect of skin on penetration into a body. Chicken skin had no effect, pig skin stopped the pellet and cow skin was perforated by the pellet. If cow skin was embedded in the gelatin there was little effect on the total amount of penetration, but cow skin on the front surface of the gelatin reduced penetration by about 30%.Computed tomography was used to examine the pellet track and to calculate the volume of damage produced. However, due to the similar densities of gelatin and organ a technique had to be developed to differentiate phases. A barium salt paste was applied to outer surfaces and iodine solution or barium nitrate solution containing red food colouring was injected into the pellet track to enhance the contrast of the track. The track through the gelatin tended to enclose itself whereas the track through the organ remained more open, presumably due to the inhomogeneity of the fibrous nature of the tissue.Pellets were also fired at construction materials (wood, plasterboard and brick) and computed tomography used to determine the volume of damage created. Pellets perforated single layers of wood and plasterboard and would embed in a second layer. However, if the two layers were in contact the pellet did not penetrate the first layer. An air rifle pellet could therefore perforate house construction materials, although the resultant kinetic energy would be low and further damage would be limited.Some of the possible physical parameters are discussed that might help predict the degree of damage caused, but from this study it is not possible to define a limit which could be proposed as safe

An Infrared Camera for Leuschner Observatory and the Berkeley Undergraduate Astronomy Lab

We describe the design, fabrication, and operation of an infrared camera which is in use at the 30-inch telescope of the Leuschner Observatory. The camera is based on a Rockwell PICNIC 256 x 256 pixel HgCdTe array, which is sensitive from 0.9-2.5 micron. The primary purpose of this telescope is for undergraduate instruction. The cost of the camera has been minimized by using commercial parts whereever practical. The camera optics are based on a modified Offner relay which forms a cold pupil where stray thermal radiation from the telescope is baffled. A cold, six-position filter wheel is driven by a cryogenic stepper motor, thus avoiding any mechanical feed throughs. The array control and readout electronics are based on standard PC cards; the only custom component is a simple interface card which buffers the clocks and amplifies the analog signals from the array.Comment: 13 pages, 17 figures. Submitted to Publications of the Astronomical Society of the Pacific: 2001 Jan 10, Accepted 2001 Jan 1

Portable reflectance spectrometer

A portable reflectance spectrometer is disclosed. The spectrometer essentially includes an optical unit and an electronic recording unit. The optical unit includes a pair of thermoelectrically-cooled detectors, for detecting total radiance and selected radiance projected through a circular variable filter wheel, and is capable of operating to provide spectral data in the range 0.4 to 2.5 micrometers without requiring coventional substitution of filter elements. The electronic recording unit includes power supplies, amplifiers, and digital recording electronics designed to permit recordation of data on tape casettes. Both the optical unit and electronic recording unit are packaged to be manually portable

The role of renal transporters and novel regulatory interactions in the TAL that control blood pressure

Hypertension (HTN), a major public health issue is currently the leading factor in the global burden of disease, where associated complications account for 9.4 million deaths worldwide every year (98). Excessive dietary salt intake is among the environmental factors that contribute to HTN, known as salt sensitivity. The heterogeneity of salt sensitivity and the multiple mechanisms that link high salt intake to increases in blood pressure are of upmost importance for therapeutic application. A continual increase in the kidney's reabsorption of sodium (Na+) relies on sequential actions at various segments along the nephron. When the distal segments of the nephron fail to regulate Na+, the effects on Na+ homeostasis are unfavourable. We propose that the specific nephron region where increased active uptake occurs as a result of variations in Na+ reabsorption is at the thick ascending limb of the loop of Henle (TAL). The purpose of this review is to urge the consideration of the TAL that contributes to the pathophysiology of salt sensitive HTN. Further research in this area will enable development of a therapeutic application for targeted treatment