Fabrication of Flexible Graphene Films for Capacitive Displacement Pressure Transducer

The long term goal of this project is to synthesize a flexible graphene film to act as an electrode for a capacitive pressure transducer. This project was commissioned by Dwyer Instruments, Inc. to replace the silicon electrode in their miniature capacitive displacement pressure transducer. Replacing silicon with graphene should yield a pressure transducer that more accurately measures low pressures and has a larger dynamic range. The first step of this project is to convert graphite to graphite oxide (GO) using permanganate and hydrogen peroxide oxidation. The resulting GO powder has been characterized using x-ray diffraction and infrared spectroscopy. Several methods are being explored to reduce the GO to form a flexible graphene-containing film. One potential method is to soak sheets of paper in an aqueous GO solution, allow the water to evaporate, and then reduce the GO by heating the film in a 250 °C oven

Baseline Data Analysis of the Throgren Basin Retrofit

The Thorgren Basin, located in Valparaiso, IN, was created as a component of the stormwater management system for the city. The basin’s primary function is to collect stormwater from two separate regions of the Salt Creek Watershed after large storm events, that is then discharged through a single outlet. In its current state the basin has limited infiltration capabilities due to a lack of vegetation and pervious surfaces. The Save the Dunes Conservation Fund, a local non-profit group, provided a cost share opportunity to naturalize Thorngren basin. The goal of the summer 2011 project was to create a monitoring strategy for the basin, collect baseline water quality data to later evaluate the effectiveness of the retrofit, and provide public education and awareness of the project. Water quality and quantity parameters were evaluated on weekly sampling events within the interior and exterior of the basin. The water quality parameters analyz ed include Total Suspended Solids (TSS), nitrates, Total Phosphorus (TP), Dissolved Oxygen (DO), pH, temperature, and conductivity. Benthic macroinvertebrates were also collected to assess the extent of biologic degradation. Water quantity was evaluated using discharge rates from the inlets and outlets of the basin, along with evaluating the retention rates after storm events. After examining the water quality parameters, the northern most inlet (21F and 21B) had the most inconsistent water chemistry values and was the main contributor to water pollutants such as nitrates, Total Suspended Solids (TSS), and E. coli bacteria. By the time pollutants reached the outlet, the data reflects a mild dilution of nutrients and sediment, whether it be absorbed by plant or macroinvertebrate life. The effectiveness of the retrofit will be noted by the decrease in these pollutants and water chemistry after future data is collected post-implementation caused by alterations in plant species and the internal structure of the basin

Hypersonic Boundary Layer Measurements with Variable Blowing Rates Using Molecular Tagging Velocimetry

Measurements of mean and instantaneous streamwise velocity profiles in a hypersonic boundary layer with variable rates of mass injection (blowing) of nitrogen dioxide (NO2) were obtained over a 10-degree half-angle wedge model. The NO2 was seeded into the flow from a slot located 29.4 mm downstream of the sharp leading edge. The top surface of the wedge was oriented at a 20 degree angle in the Mach 10 flow, yielding an edge Mach number of approximately 4.2. The streamwise velocity profiles and streamwise fluctuating velocity component profiles were obtained using a three-laser NO2->NO photolysis molecular tagging velocimetry method. Observed trends in the mean streamwise velocity profiles and profiles of the fluctuating component of streamwise velocity as functions of the blowing rate are described. An effort is made to distinguish between the effect of blowing rate and wall temperature on the measured profiles. An analysis of the mean velocity profiles for a constant blowing rate is presented to determine the uncertainty in the measurement for different probe laser delay settings. Measurements of streamwise velocity were made to within approximately 120 gm of the model surface. The streamwise spatial resolution in this experiment ranged from 0.6 mm to 2.6 mm. An improvement in the spatial precision of the measurement technique has been made, with spatial uncertainties reduced by about a factor of 2 compared to previous measurements. For the quiescent flow calibration measurements presented, uncertainties as low as 2 m/s are obtained at 95% confidence for long delay times (25 gs). For the velocity measurements obtained with the wind tunnel operating, average single-shot uncertainties of less than 44 m/s are obtained at 95% confidence with a probe laser delay setting of 1 gs. The measurements were performed in the 31-inch Mach 10 Air Tunnel at the NASA Langley Research Center

P-11 Social Support and Fundamentalism as Predictors of Religious Internalization

What variables are the strongest predictors of religious identification? Religious internalization can be broken down into identification, the full adoption of religious values, and introjection, a partial internalization of values. Fundamentalism is one variable thought to predict these motivational types. We defined fundamentalism as the degree to which someone holds their sacred texts as true. Additionally, social support relates to religious internalization. To measure these variables, two separate scales were used for each: Multidimensional Scale of Perceived Social Support and Basic Psychological Need Satisfaction Scale for social support, Intratextual Fundamentalism Scale and Revised Religious Fundamentalism scale for fundamentalism, and The Faith Maturity Scale and Christian Religious Internalization Scale for religious internalization. After extensive review of previous literature, it was hypothesized that measures of fundamentalism would be independent predictors of religious identification relative to social support. The results of this study were analyzed using a hierarchical linear regression and the results of this hypothesis were partially supported. Fundamental beliefs and a personal relationship with Christ were the predictors of religious identification after measures of social support were included; however, measures of social support accounted for minimal additional variance

Veterinary antibiotic sorption to agroforestry buffer, grass buffer and cropland soils

Paper presented at the 11th North American Agroforesty Conference, which was held May 31-June 3, 2009 in Columbia, Missouri.In Gold, M.A. and M.M. Hall, eds. Agroforestry Comes of Age: Putting Science into Practice. Proceedings, 11th North American Agroforestry Conference, Columbia, Mo., May 31-June 3, 2009.Veterinary antibiotics are used to treat infectious animal diseases and enhance animal growth. In Missouri, the increased growth of confined animal feeding operations (CAFOs) and the need to dispose of manure generated by CAFOS may be problematic due to co-application of antibiotics during land application of manure. Surface runoff events from claypan or claypan-like soils are relatively frequent; thus, there is a need to develop and evaluate the use of vegetative buffer strips (VBS) as management tools to reduce antibiotic transport to surface water resources. The objectives of this study were to (1) investigate oxytetracycline (OTC) and sulfadimethoxine (SDT) sorption to agroforestry (tree/grass) buffer, grass buffer, and cropland soils, (2) evaluate differences in antibiotic sorption between soils collected from different vegetative species, and (3) elucidate relationships between soil properties and antibiotic sorption. Sorption/desorption isotherms generated using batch techniques were well-fitted by the Freundlich isotherm model (r2 [greater than] 0.80). Oxytetracycline was strongly adsorbed by all soils, and the antibiotic was not readily desorbed; hysteresis was observed between all adsorption and desorption isotherms. Solid-solution distribution coefficients (Kd) values of OTC are an order of magnitude greater than those of SDT. Statistical analyses indicate that OTC Kd values are significantly greater for VBS soils relative to cropland soil, and STD Kd values are significantly greater for agroforestry soils as compared to other soils studied. Regression analyses correlating antibiotic sorption to soil properties are in progress. Results indicate that agroforestry and grass buffers may effectively mitigate antibiotic loss from agroecosystems due to enhanced antibiotic sorption properties.Bei Chu (1), Keith Goyne (1), Stephen H. Anderson (1), Ranjith P. Udawatta (2) and Chung-Ho Lin (2) ; 1. Department of Soil, Environmental and Atmospheric Sciences, University of Missouri, 302 ABNR Building, Columbia, MO 65211. 2. Center for Agroforestry, University of Missouri, 203 ABNR, Columbia, MO 65211.Includes bibliographical references

Hypersonic Boundary Layer Transition Measurements Using NO2 approaches NO Photo-dissociation Tagging Velocimetry

Measurements of instantaneous and mean streamwise velocity profiles in a hypersonic laminar boundary layer as well as a boundary layer undergoing laminar-to-turbulent transition were obtained over a 10-degree half-angle wedge model. A molecular tagging velocimetry technique consisting of a NO2 approaches?NO photo-dissociation reaction and two subsequent excitations of NO was used. The measurement of the transitional boundary layer velocity profiles was made downstream of a 1-mm tall, 4-mm diameter cylindrical trip along several lines lying within a streamwise measurement plane normal to the model surface and offset 6-mm from the model centerline. For laminar and transitional boundary layer measurements, the magnitudes of streamwise velocity fluctuations are compared. In the transitional boundary layer the fluctuations were, in general, 2-4 times larger than those in the laminar boundary layer. Of particular interest were fluctuations corresponding to a height of approximately 50% of the laminar boundary layer thickness having a magnitude of nearly 30% of the mean measured velocity. For comparison, the measured fluctuations in the laminar boundary layer were approximately 5% of the mean measured velocity at the same location. For the highest 10% signal-to-noise ratio data, average single-shot uncertainties using a 1 ?Es and 50 ?Es interframe delay were ~115 m/s and ~3 m/s, respectively. By averaging single-shot measurements of the transitional boundary layer, uncertainties in mean velocity as low as 39 m/s were obtained in the wind tunnel. The wall-normal and streamwise spatial resolutions were 0.14-mm (2 pixel) and 0.82-mm (~11 pixels), respectively. These measurements were performed in the 31-inch Mach 10 Air Wind Tunnel at the NASA Langley Research Center

Islet Autoantibody Measurements from Dried Blood Spots on Filter Paper Strongly Correlate to Serum Levels

Type 1 diabetes (T1D) is increasing in incidence and predictable with measurement of serum islet autoantibodies (iAb) years prior to clinical disease onset. Identifying iAb positive individuals reduces diabetic ketoacidosis and identifies individuals for T1D prevention trials. However, large scale screening for iAb remains challenging as assays have varying sensitivities and specificities, insulin autoantibodies remain difficult to measure and venipuncture is generally required to obtain serum. We developed an approach to reliably measure all four major iAb, including insulin autoantibodies, from dried blood spots (DBS) on filter-paper. By spiking iAb positive serum into iAb negative whole blood in a dose titration, we optimized the conditions for autoantibody elution from filter paper as measured by fluid phase radioimmunoassays. After assessing stability of measuring iAb from DBS over time, we then screened iAb from DBS and the corresponding serum in new-onset T1D (n = 52), and controls (n = 72) which included first-degree relatives of T1D patients. iAb measured from eluted DBS in new-onset T1D strongly correlated with serum measurements (R2 = 0.96 for mIAA, GADA = 0.94, IA-2A = 0.85, ZnT8A = 0.82, p<0.01 for each autoantibody). There were no false positives in control subjects, and 5/6 with previously unknown iAb positivity in sera were detected using DBS. With further validation, measuring iAb from DBS can be a reliable method to screen for T1D risk

ABCC6 plays a significant role in the transport of nilotinib and dasatinib, and contributes to TKI resistance in vitro, in both cell lines and primary patient mononuclear cells

ATP Binding Cassette family efflux proteins ABCB1 and ABCG2 have previously been demonstrated to interact with Tyrosine Kinase Inhibitors (TKIs); however, evidence for the interaction of other potentially relevant drug transporters with TKIs is lacking. Through Taqman transporter array technology we assessed the impact of nilotinib on mRNA expression of ABC transporters, with ABCC6 identified as a transporter of interest. Additionally, increased expression of ABCC6 mRNA was observed during in vitro development of nilotinib resistance in BCR-ABL1-expressing cell lines. K562 cells exposed to gradually increasing concentrations of nilotinib (to 2 μM) expressed up to 57-fold higher levels of ABCC6 mRNA when compared with control cells (p = 0.002). Analogous results were observed in nilotinib resistant K562-Dox cells (up to 33-fold higher levels of ABCC6, p = 0.002). IC50 experiments were conducted on patient mononuclear cells in the absence and presence of three ABCC6 inhibitors: indomethacin, probenecid and pantoprazole. Results demonstrated that all three inhibitors significantly reduced nilotinib IC50 (p<0.001) indicating ABCC6 is likely involved in nilotinib transport. Cell line data confirmed these findings. Similar results were obtained for dasatinib, but not imatinib. Combined, these studies suggest that nilotinib and dasatinib are likely substrates of ABCC6 and to our knowledge, this is the first report of ABCC6 involvement in TKI transport. In addition, ABCC6 overexpression may also contribute to nilotinib and dasatinib resistance in vitro. With nilotinib and dasatinib now front line therapy options in the treatment of CML, concomitant administration of ABCC6 inhibitors may present an attractive option to enhance TKI efficacy.Laura N. Eadie, Phuong Dang, Jarrad M. Goyne, Timothy P. Hughes, Deborah L. Whit