Starting Day Care in Espoo from the Viewpoint of Chinese Parents

The thesis is a small-scale evaluative research. The day care start folder, which provided practical and detailed information about starting Finnish day care to support multicultural parents in Otaniemi day-care centre and Servin-Maija day-care centre, was assessed among nine Chinese parents of children in five municipal and outsourced day-care centres in the City of Espoo. The purposes of the current research were to find out how Chinese parents perceive starting day care in the City of Espoo and their difficulties and suggestions concerning starting day care in the City of Espoo; to evaluate how the day care start folder is experienced as a working method in opinion of Chinese parents living in the City of Espoo; and to further improve the day care start folder as a working method for multicultural families and personnel in Otaniemi day-care centre and possibly for other day-care centres in the similar situation. Two types of interview, namely semi-structured individual interview and focus group interview, were utilised as the primary qualitative data-collecting methods in the current research. In addition, questionnaire as an assistant quantitative data-collecting method was employed as well to ensure verification of the consistency of the data, and supplement the abundance of the narrative data collected from interviews. The qualitative data gathered from the six interviews was coded, categorised and analysed in accordance with the method of content analysis. The results showed that the participating Chinese parents basically concerned about the information on starting day care; their child’s language learning; their communication and cooperation with day-care staff; cultures, religions and festivals; playing, learning and friends; child protection and legislation and so forth. Furthermore, these Chinese parents also put forward some important problems and suggestions on Finnish day care, such as problems caused by cultural differences, different opinions on learning by playing and Finnish as a second language-teaching, difficulties to obtain enough English information about Finnish day care, and the lack of mediums for foreign families to receive help concerning Finnish day care. In addition, these Chinese parents perceived the day care start folder as useful and adequate. The results based on the questionnaires supported and verified the results generated from the qualitative data analysis

A novel copper complex induces ROS generation in doxorubicin resistant Ehrlich ascitis carcinoma cells and increases activity of antioxidant enzymes in vital organs in vivo

BACKGROUND: In search of a suitable GSH-depleting agent, a novel copper complex viz., copper N-(2-hydroxyacetophenone) glycinate (CuNG) has been synthesized, which was initially found to be a potential resistance modifying agent and later found to be an immunomodulator in mice model in different doses. The objective of the present work was to decipher the effect of CuNG on reactive oxygen species (ROS) generation and antioxidant enzymes in normal and doxorubicin-resistant Ehrlich ascites carcinoma (EAC/Dox)-bearing Swiss albino mice. METHODS: The effect of CuNG has been studied on ROS generation, multidrug resistance-associated protein1 (MRP1) expression and on activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). RESULTS: CuNG increased ROS generation and reduced MRP1 expression in EAC/Dox cells while only temporarily depleted glutathione (GSH) within 2 h in heart, kidney, liver and lung of EAC/Dox bearing mice, which were restored within 24 h. The level of liver Cu was observed to be inversely proportional to the level of GSH. Moreover, CuNG modulated SOD, CAT and GPx in different organs and thereby reduced oxidative stress. Thus nontoxic dose of CuNG may be utilized to reduce MRP1 expression and thus sensitize EAC/Dox cells to standard chemotherapy. Moreover, CuNG modulated SOD, CAT and and GPx activities to reduce oxidative stress in some vital organs of EAC/Dox bearing mice. CuNG treatment also helped to recover liver and renal function in EAC/Dox bearing mice. CONCLUSION: Based on our studies, we conclude that CuNG may be a promising candidate to sensitize drug resistant cancers in the clinic

Evi1 regulates the expression of other CHD genes during embryonic heart development.

<p>(A) The number of CHD genes represented in Evi1 ChIP-Seq data (Evi1 bound genes) or in the list of genes regulated by Mecom. An enriched number of CHD genes were found bound or regulated by Mecom (50 out of 143 genes), p = 0.0453 and p = 0.0276, respectively. These genes represent potential Mecom target genes in heart development. (B) Mecom regulates the expression of 23 CHD genes, which contain Evi1-binding sites specifically in heart. Heart and head (neural crest) tissues were harvested from WT and Evi1^δex3/δex3 embryos of somite number 9 to 18. RT-qPCR assays were performed. Genes considered to be mis-regulated in Evi1^δex3/δex3 hearts were increased or decreased in expression by at least three fold in average for all samples of the same time-point. These graphs are representative of two to five independent experiments.</p

Overview of Major Reported Expression Domains.

<p><b>Key</b></p><p>AA – Aortic Arch and Aortic Arch Arteries.</p><p>CC/HT - Cardiac Crescent/Heart Tube.</p><p>End – Endocardium (+Csn – including Cushions).</p><p>Myo – Myocardium.</p><p>NC –Neural Crest (Cardiac).</p><p>OFT – Outflow Tract.</p><p>SHF – Secondary Heart Field.</p

Expression of Mecom mRNA in cardiac structures of wild type embryos.

<p>(A–D) Whole mount mRNA <i>in situ</i> hybridization to show Mecom expression. A–C) Expression during subsequent stages of heart tube formation E8.5 (black brackets). D) At E9.5 Evi1 is expressed in the endothelial cells and in the endocardium of the heart and in the mesenchyme of the aortic arches. Expression also includes a population of migrating neural crest cells (white arrowhead). E–J) E10.5 Sagittal sections (from right to left) showing Evi1 in the aortic arches (a), mesenchyme of the secondary heart field (black arrowheads), outflow and atrio-ventricular canal endocardium including the cushions.</p

Spontaneous lethal bone marrow depletion in mice harboring an Evi1 exon3 deletion in the hematopoietic system.

<p>(A) Histology was performed on sick Vav-iCre; Evi1^fl3/fl3 and littermate control mice. Bone marrow depletion was observed in the mutant mice. Adipose tissue replaced the hematopoietic cells in the bone marrow. (B) Increased erythropoiesis in the spleen of Vav-iCre; Evi1^fl3/fl3 mice. No visible border was found between the red pulp and white pulp. Erythroid cells are shown by the arrows. Excess erythropoiesis in spleen likely happens to compensate for bone marrow loss. (C) H&E stained sections of the brain of a dying Vav-iCre; Evi1^fl3/fl3 mouse. Hemorrhages (red areas) were visible at several locations (also see Fig. S3E in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0089397#pone.0089397.s001" target="_blank">File S1</a>). (D) Histological sections of tissues from dying Vav-iCre; Evi1^fl3/fl3 animals showing bacteremia. Red arrows indicate the presence of bacteria in alveolar capillaries. Giemsa stains reveal the presence of cocci or small rods within glomerular capillaries. No sign of immune system defense (inflammatory cells) was observed despite the infection.</p

Deletion of Evi1 exon3 generates a hypomorphic allele.

<p>(A) Sequenced products obtained after 5′RACE from wild type or Evi1^δex3/δex3 mutant embryos. (B) Table showing the fraction of embryos of each genotype detected at different stages of embryonic development. The Mendelian ratios were not affected by the Evi1 exon3 deletion. (C) Pictures of 28 hr-old littermates highlight the poor health of dying Evi1^δex3/δex3 pups. (D) Kaplan-Meyer curves for wild type, Evi1^δex3/+ and Evi1^δex3/δex3 progeny indicate lethality of all Evi1^δex3/δex3 pups by three days after birth (n = 5 to 16 per genotype). Log rank test, Chi square p value <0.0001. (E) RT-qPCR from cDNA of E14.5 embryos. The primers used amplified the regions between Evi1 exons 2 and 3, 3 and 4 or 13 and 14. Mean of three different samples per condition. The standard deviation is shown. (F) Expression of Evi1 and γ-tubulin protein products in E14.5 wild type or E17.5 Evi1^δex3/+ and Evi1^δex3/δex3 mutant embryos (100 µg protein/lane). (G) Nucleotide sequence of Evi1 cDNA in the exon 3 and 4 genomic region. Two ATG sites are present in exon 3 and one in exon 4. All ATGs are conserved in higher vertebrates.</p

Cardiac malformations and failure in Evi1^δex3/δex3 mice.

<p>(A) Transverse sections and (B) 3D reconstruction (left-ventral oblique view) of hearts from Evi1^δex3/δex3 or wild type littermate (+/+) E15.5 embryos analyzed by magnetic resonance imaging (MRI). The aorta (Ao), right ventricle (RV), left ventricle (LV), ventricular septum (VS), trachea (Tr), aortic arch (AoA) and ductus arteriosus (DA) are indicated. Ventricular septal defect (VSD), interrupted aortic arch (IAA) and common arterial trunk (CAT) were observed in Evi1^δex3/δex3 hearts. (C) List of the congenital heart defects identified in fifteen E15.5 embryos of various different genotypes by MRI and 3D reconstruction. (D) Hematoxylin and eosin staining of 5 µm sections of a sick Evi1^δex3/δex3 pup. Subcutaneous and other tissue edema (white spaces) was present, consistent with heart failure.</p