3 research outputs found
Expression and role of PAICS, a de novo purine biosynthetic gene in prostate cancer
Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/143605/1/pros23533_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/143605/2/pros23533.pd
Comparative Functional Analysis of Rat and Xenopus laevis Promoters Suggest Differential Regulations
We have carried out a comparative functional analysis of the rat and Xenopus laevis promoters across several mammalian and amphibian cell lines. Progressive deletion constructs of both the promoters have been made using a PCR based approach and the basal promoter activities studied in Xenopus tadpole cell line (XTC), Xenopus adult kidney fibroblast cell line (A6), human hepatoma cell line (HepG2), normal rat kidney cell line (NRK), and Chinese hamster ovary cell line (CHO). Data suggests that the basal promoter activity of is low as compared to promoter in XTC cells but comparable in A6 cells, while promoter shows nearly negligible activity as compared to TGF-beta5 promoter in all the tested mammalian cell lines. Moreover, promoter is found to be repressed in XTC cells on treatment with protein. Thus, the regulation of and promoters is distinct in amphibian and mammalian species. We therefore suggest that contrary to the suggested functional equivalence of and proteins, and genes have distinct functions in their respective species