Sclerochronological studies in the humboldt current system, a highly variable ecosystem

The Humboldt Current that bathes the west coast of South America is affected by different influences at daily to decadal periodicities. Environmental influences such as upwelling or coastal trapped waves as well as climate influences such as El Nino southern oscillation and Pacific decadal oscillation events interact and modify the thermonutricline depth of this Humboldt Current System. The position of this thermonutricline plays a key role in Humboldt Current System functioning by driving sea surface temperature (SST) and primary production variations. As filter feeders, bivalves are particularly affected by SST and primary production, and their shells can provide information about these two environmental factors. Using sclerochronology, we studied three bivalve species from different ecological niches living along the Peruvian-Chilean coast. Depending on the species, thick or thin sections, etched or not, were studied using an optical microscope. Increment thicknesses were measured at the surface of the shell and/or in cross-section. Moreover, the mineralogical composition of the different layers of the studied shells was determined on thin sections by Fourier transform infrared spectroscopic analyses. Growth results were then interpreted tentatively in terms of environmental variations. For the free-living, short-lived Pectinidae Argopecten purpuratus, the daily growth rhythm is linked to the solar period whereas growth amplitude seems to be related to the occurrence of spawning events, probably triggered by specific water temperature patterns. tidal regime and SST seem to be the major environmental parameters that govern shell growth rhythms and/or increment thickness for two nearshore species-the Mytilidae Choromytilus chorus and the Veneridae Eurhomalea rufa-which grow more slowly and have a life span longer than A. purpuratus, and have a strong potential for paleo-environmental and paleo-El Nino southern oscillation reconstruction. These results need to be confirmed studying multiple shells to quantify individual growth variations and to enhance the significance of the findings on the different environmental parameters recorded in the growth patterns of the South American bivalve species studied

Ageing prolongs inflammatory marker expression in regenerating rat skeletal muscles after injury

<p>Abstract</p> <p>Background</p> <p>Some of the most serious consequences of normal ageing relate to its effects on skeletal muscle, particularly significant wasting and associated weakness, termed "sarcopenia". The underlying mechanisms of sarcopenia have yet to be elucidated completely but an altered muscle inflammatory response after injury is a likely contributing factor. In this study we investigated age-related changes in the expression of numerous inflammatory markers linked to successful muscle regeneration.</p> <p>Methods</p> <p>Right extensor digitorum longus (EDL) muscles from young (3 month), adult (12 month) and old (24 month) male F344 rats were injected with bupivacaine hydrochloride to cause complete muscle fibre degeneration, then excised 12, 24, 36, and 72 hours later (n = 5/age group/time point). We used qRT-PCR to quantify the mRNA expression levels of the inflammatory markers TNFα, IFNγ, IL1, IL18, IL6, and CD18 as well as regenerative markers MyoD and myogenin.</p> <p>Results</p> <p>Inflammatory markers were all increased significantly in all age groups after myotoxic injury. There was a trend for expression of inflammatory markers to be higher in uninjured muscles of old rats, especially at 72 hours post injury where the expression levels of several markers was significantly higher in old compared with young and adult rats. There was also a decrease in the expression of regenerative markers in old rats at 72 hours post injury.</p> <p>Conclusion</p> <p>Our findings identify a prolonged inflammatory signature in injured muscles from old compared with young and adult rats together with a blunted expression of key markers of regeneration in muscles of old rats. Importantly, our findings identify potential targets for future therapeutic strategies for improving the regenerative capacity of skeletal muscle during ageing.</p

Effect of and Acute Bout of Kettlebell Exercise on Glucose Tolerance in Sedentary Men: a Preliminary Study

International Journal of Exercise Science 9(4): 524-535, 2016. Impaired glucose tolerance can have significant health consequences. The purposes of this preliminary study were to examine whether a single session of kettlebell exercise improves acute post-exercise glucose tolerance in sedentary individuals, and whether it was as effective as high-intensity interval running. Six sedentary male subjects underwent a two-hour oral glucose tolerance test following three different conditions: 1) control (no exercise); 2) kettlebell exercise (2 sets of 7 exercises, 15 repetitions per exercise with 30 seconds rest between each exercise); or 3) high-intensity interval running (10 one-minute intervals at a workload corresponding to 90% VO2max interspersed with one-minute active recovery periods). Blood glucose and insulin levels were measured before (0 minutes), and 60 and 120 minutes after glucose ingestion. Both kettlebell and high-intensity interval running exercise significantly lowered blood glucose 60 minutes after glucose ingestion compared with control. However, there was no significant difference in blood glucose between the two exercise conditions at any time point. In addition, there were no significant differences in insulin concentration between high intensity interval running, kettlebell, and control conditions at all time points. Results indicate that an acute bout of kettlebell exercise is as effective as high intensity interval running at improving glucose tolerance in sedentary young men