58 research outputs found
Chemical Detection of Short-Lived Species Induced in Aqueous Media by Atmospheric Pressure Plasma
Non-thermal atmospheric pressure plasmas are widely used in biomedical research and clinical applications. Such plasmas generate a variety of reactive oxygen and nitrogen species upon interaction with ambient surroundings. These species further interact with a biological substrate and are responsible for the biomedical effects of plasma. Liquid water is an essential part of any biological systems. Some of the most reactive species induced by plasma in aqueous media are radicals and atoms. Hence, the presence of certain chemical components in a plasma ‘cocktail’ presents an important task for both understanding and further development of plasma systems with specific purposes. In this chapter, we discuss various methods of detection of the plasma-generated short-lived reactive species. We dissert various plasma-induced radicals and atoms (•OH, O2•−/•OOH, •NO, O), together with non-radical short-lived species (−OONO, O3, 1O2). Electron paramagnetic resonance (EPR) is the most direct method of radical detection in water-based media. Special attention is paid to the limitations of the detection methods, with an emphasis on spin trapping used in EPR analysis
Plasma-treated liquids in medicine: let's get chemical
Fundamental and applied research on plasma-treated liquids for biomedical applications was boosted in the last few years, dictated by their advantages with respect to direct treatments. However, often, the lack of consistent analysis at a molecular level of these liquids, and of the processes used to produce them, have raised doubts of their usefulness in the clinic. The aim of this article is to critically discuss some basic aspects related to the use of plasma-treated liquids in medicine, with a focus on their chemical composition. We analyze the main liquids used in the field, how they are affected by non-thermal plasmas, and the possibility to replicate them without plasma treatment.Peer ReviewedObjectius de Desenvolupament Sostenible::3 - Salut i BenestarPostprint (published version
Non-target Biomolecules Alter Macromolecular Changes Induced by Bactericidal Low-temperature Plasma
Investigation of plasma-induced chemistry in organic solutions for enhanced electrospun PLA nanofibers
Electrospinning is a versatile technique for the fabrication of polymer-based nano/microfibers. Both physical and chemical characteristics of pre-electrospinning polymer solutions affect the morphology and chemistry of electrospun nanofibers. An atmospheric-pressure plasma jet has previously been shown to induce physical modifications in polylactic acid (PLA) solutions. This work aims at investigating the plasma-induced chemistry in organic solutions of PLA, and their effects on the resultant PLA nanofibers. Therefore, very broad range of gas, liquid, and solid (nanofiber) analyzing techniques has been applied. Plasma alters the acidity of the solutions. SEM studies illustrated that complete fiber morphology enhancement only occurred when both PLA and solvent molecules were exposed to pre-electrospinning plasma treatment. Additionally, the surface chemistry of the PLA nanofibers was mostly preserved
Oxidative stress in healthy pregnancy and preeclampsia is linked to chronic inflammation, iron status and vascular function
Background During normal pregnancy, placental oxidative stress (OS) is present during all three trimesters and is necessary to obtain normal cell function. However, if OS reaches a certain level, pregnancy complications might arise. In preeclampsia (PE), a dangerous pregnancy specific hypertensive disorder, OS induced in the ischemic placenta causes a systemic inflammatory response and activates maternal endothelial cells. In this study, we aimed to quantify superoxide concentrations (as a measure of systemic OS) using electron paramagnetic resonance (EPR) and correlate them to markers of systemic inflammation, iron status and vascular function. Methods Fifty-nine women with a healthy pregnancy (HP), 10 non-pregnant controls (NP) and 28 PE patients (32±3.3weeks) were included. During HP, blood samples for superoxide, neutrophil to lymphocyte ratio (NLR), mean platelet volume (MPV) and iron status were taken at 10, 25 and 39 weeks. Vascular measurements for arterial stiffness (carotid-femoral pulse wave velocity (CF-PWV), augmentation index (AIx), augmentation Pressure (AP)) and microvascular endothelial function (reactive hyperemia index (RHI)) were performed at 35 weeks. In PE, all measurements were performed at diagnosis. CMH (1-hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine) was used as spin probe for EPR, since the formed CM radical corresponds to the amount of superoxide. Results Superoxide concentration remains stable during pregnancy (p = 0.92), but is significantly higher compared to the NP controls (p<0.0001). At 25 weeks, there is a significant positive correlation between superoxide and ferritin concentration. (p = 0.04) In PE, superoxide, systemic inflammation and iron status are much higher compared to HP (all p<0.001). During HP, superoxide concentrations correlate significantly with arterial stiffness (all p<0.04), while in PE superoxide is significantly correlated to microvascular endothelial function (p = 0.03). Conclusions During HP there is an increased but stable oxidative environment, which is correlated to ferritin concentration. If superoxide levels increase, there is an augmentation in arterial stiffness. In PE pregnancies, systemic inflammation and superoxide concentrations are higher and result in a deterioration of endothelial function. Together, these findings support the hypothesis that vascular function is directly linked to the amount of OS and that measurement of OS in combination with vascular function tests might be used in the prediction of PE
Chapter Chemical Detection of Short-Lived Species Induced in Aqueous Media by Atmospheric Pressure Plasma
Non-thermal atmospheric pressure plasmas are widely used in biomedical research and clinical applications. Such plasmas generate a variety of reactive oxygen and nitrogen species upon interaction with ambient surroundings. These species further interact with a biological substrate and are responsible for the biomedical effects of plasma. Liquid water is an essential part of any biological systems. Some of the most reactive species induced by plasma in aqueous media are radicals and atoms. Hence, the presence of certain chemical components in a plasma ‘cocktail’ presents an important task for both understanding and further development of plasma systems with specific purposes. In this chapter, we discuss various methods of detection of the plasma-generated short-lived reactive species. We dissert various plasma-induced radicals and atoms (•OH, O2•−/•OOH, •NO, O), together with non-radical short-lived species (−OONO, O3, 1O2). Electron paramagnetic resonance (EPR) is the most direct method of radical detection in water-based media. Special attention is paid to the limitations of the detection methods, with an emphasis on spin trapping used in EPR analysis
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