A Dense Reference Network for Mass-Market Centimeter-Accurate Positioning

The quality of atmospheric corrections provided by a dense reference network for centimeter-accurate carrierphase differential GNSS (CDGNSS) positioning is investigated. A dense reference network (less than 20 km inter-station distance) offers significant benefits for mass-market users, enabling lowcost (including single-frequency) CDGNSS positioning with rapid integer ambiguity resolution. Precise positioning on a massmarket platform would significantly influence the world economy, ushering in a host of consumer-focused applications such as globally-registered augmented and virtual reality and improved all-weather safety and efficiency for intelligent transportation systems, applications which have so far been hampered by the several-meter-level errors in standard GNSS positioning. This contribution examines CDGNSS integer ambiguity resolution performance in terms of network correction uncertainty, and network correction uncertainty, in turn, in terms of network density. It considers the total error in network corrections: a sum of ionospheric, tropospheric, and reference station multipath components. The paper’s primary goal is to identify the network density beyond which mass-market users would see no further significant improvement in ambiguity resolution performance. It finishes by describing development and deployment of a low-cost dense reference network in Austin, Texas.Aerospace Engineering and Engineering Mechanic

Combining community-based research and local knowledge to confront asthma and subsistence-fishing hazards in Greenpoint/Williamsburg, Brooklyn, New York.

Activists in the environmental justice movement are challenging expert-driven scientific research by taking the research process into their own hands and speaking for themselves by defining, analyzing, and prescribing solutions for the environmental health hazards confronting communities of the poor and people of color. I highlight the work of El Puente and The Watchperson Project--two community-based organizations in the Greenpoint/Williamsburg neighborhood in Brooklyn, New York, that have engaged in community-based participatory research (CBPR) to address asthma and risks from subsistence-fish diets. The CBPR process aims to engage community members as equal partners alongside scientists in problem definition, information collection, and data analysis--all geared toward locally relevant action for social change. In the first case I highlight how El Puente has organized residents to conduct a series of asthma health surveys and tapped into local knowledge of the Latino population to understand potential asthma triggers and to devise culturally relevant health interventions. In a second case I follow The Watchperson Project and their work surveying subsistence anglers and note how the community-gathered information contributed key data inputs for the U.S. Environmental Protection Agency Cumulative Exposure Project in the neighborhood. In each case I review the processes each organization used to conduct CBPR, some of their findings, and the local knowledge they gathered, all of which were crucial for understanding and addressing local environmental health issues. I conclude with some observations about the benefits and limits of CBPR for helping scientists and communities pursue environmental justice

Circumnuclear stellar population, morphology and environment of Seyfert 2 galaxies: an evolutionary scenario

We investigate the relation between the characteristics of the circumnuclear stellar population and both the galaxy morphology and the presence of close companions for a sample of 35 Seyfert 2 nuclei. Fifteen galaxies present unambiguous signatures of recent episodes of star formation within $\approx$300 pc from the nucleus. When we relate this property with the Hubble type of the host galaxy, we find that the incidence of recent circumnuclear star formation increases along the Hubble sequence, and seems to be larger than in non-Seyfert galaxies for the early Hubble types S0 and Sa, but similar to that in non-Seyfert galaxies for later Hubble types. Both in early-type and late-type Seyferts, the presence of recent star-formation is related to the galaxy morphology in the inner few kiloparsecs, as observed in HST images through the filter F606W by Malkan et al., who has assigned a late ``inner Hubble type'' to most Seyfert 2s with recent nuclear star-formation. This new classification is due to the presence of dust lanes and spiral structures in the inner region. The presence of recent star formation in Seyfert 2 nuclei is also related to interactions: among the 13 galaxies of the sample with close companions or in mergers, 9 have recent star formation in the nucleus. These correlations between the presence of companions, inner morphology and the incidence of recent star formation suggest an evolutionary scenario in which the interaction is responsible for sending gas inwards which both feeds the AGN and triggers star-formation. The starburst then fades with time and the composite Seyfert 2 + Starburst nucleus evolves to a ``pure'' Seyfert 2 nucleus with an old stellar population.Comment: 12 pages, 6 figures, 2 table

Systems Integration and Test of the Lunar Flashlight Spacecraft

Lunar Flashlight is a 6U CubeSat launching in late 2022 or early 2023 that will search for surface water ice content in permanently shadowed regions at the south pole of the Moon using infrared relative reflectance spectroscopy. The mission will act as a technology demonstration of an Advanced Spacecraft Energetic NonToxic (ASCENT) green propulsion system and active laser spectroscopy within the CubeSat form-factor. This paper provides an overview of the entire Systems Integration and Test campaign which took place at the Jet Propulsion Laboratory and the Georgia Institute of Technology. From initial testing of the isolated avionics and payload subsystems to the final tests with a fully integrated spacecraft, the project’s integration and test campaign is reviewed, with a focus on lessons learned

Cdk1 inactivation terminates mitotic checkpoint surveillance and stabilizes kinetochore attachments in anaphase

Two mechanisms safeguard the bipolar attachment of chromosomes in mitosis. A correction mechanism destabilizes erroneous attachments that do not generate tension across sister kinetochores [1]. In response to unattached kinetochores, the mitotic checkpoint delays anaphase onset by inhibiting the anaphase-promoting complex/cyclosome (APC/CCdc20) [2]. Upon satisfaction of both pathways, the APC/CCdc20 elicits the degradation of securin and cyclin B [3]. This liberates separase triggering sister chromatid disjunction and inactivates cyclin-dependent kinase 1 (Cdk1) causing mitotic exit. How eukaryotic cells avoid the engagement of attachment monitoring mechanisms when sister chromatids split and tension is lost at anaphase is poorly understood [4]. Here we show that Cdk1 inactivation disables mitotic checkpoint surveillance at anaphase onset in human cells. Preventing cyclin B1 proteolysis at the time of sister chromatid disjunction destabilizes kinetochore-microtubule attachments and triggers the engagement of the mitotic checkpoint. As a consequence, mitotic checkpoint proteins accumulate at anaphase kinetochores, the APC/CCdc20 is inhibited, and securin reaccumulates. Conversely, acute pharmacological inhibition of Cdk1 abrogates the engagement and maintenance of the mitotic checkpoint upon microtubule depolymerization. We propose that the simultaneous destruction of securin and cyclin B elicited by the APC/CCdc20 couples chromosome segregation to the dissolution of attachment monitoring mechanisms during mitotic exit

Interfacial Self-Assembly to Spatially Organize Graphene Oxide Into Hierarchical and Bioactive Structures

Multicomponent self-assembly holds great promise for the generation of complex and functional biomaterials with hierarchical microstructure. Here, we describe the use of supramolecular co-assembly between an elastin-like recombinamer (ELR5) and a peptide amphiphile (PA) to organise graphene oxide (GO) flakes into bioactive structures across multiple scales. The process takes advantage of a reaction – diffusion mechanism to enable the incorporation and spatial organization of GO within multiple ELR5/PA layers. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), and ImageJ software were used to demonstrate the hierarchical organisation of GO flakes within the ELR5/PA layers and the distribution profiles of GO throughout the ELR5/PA membranes. Furthermore,atomic force microscopy (AFM) revealed improved Young’s moduli of the ELR5/PA/GOmembranes compared to the ELR5/PA membranes. Lastly, we investigated biocompatibility of the ELR5/PA/GO membrane via various cell culture methods

On the Origin of Broad Fe K alpha and Hi H alpha Lines in AGN

We examine the properties of the Fe emission lines that arise near 6.4 keV in the ASCA spectra of AGN. Our emphasis is on the Seyfert 1 galaxies where broad and apparently complex Fe K alpha emission is observed. We consider various origins for the line but focus on the pros and cons for line emitting accretion disk models. We develop a simple model of an illuminated disk capable of producing both X-ray and optical lines from a disk. The model is able to reproduce the observed Fe K alpha FWHM ratio as well as the radii of maximum emissivity implied by the profile redshifts. The overall profile shapes however do not fit well the predictions of our disk illumination model nor do we derive always consistent disk inclinations for the two lines. We conclude that the evidence for and against an accretion disk origin for the Fe K alpha emission is equal at best. The bulk of the data requires a very disparate set of line fits which shed little light on a coherent physical model. We briefly consider alternatives to disk emission models and show that a simple bicone model can reproduce the FE line profiles equally well.Comment: 29 pages, 6 tables, 6 figures. Submitted for publication in the Astrophysical Journal part

CD32 is expressed on cells with transcriptionally active HIV but does not enrich for HIV DNA in resting T cells

The persistence of HIV reservoirs, including latently infected, resting CD4+ T cells, is the major obstacle to cure HIV infection. CD32a expression was recently reported to mark CD4+ T cells harboring a replication-competent HIV reservoir during antiretroviral therapy (ART) suppression. We aimed to determine whether CD32 expression marks HIV latently or transcriptionally active infected CD4+ T cells. Using peripheral blood and lymphoid tissue of ART-treated HIV+ or SIV+ subjects, we found that most of the circulating memory CD32+ CD4+ T cells expressed markers of activation, including CD69, HLA-DR, CD25, CD38, and Ki67, and bore a TH2 phenotype as defined by CXCR3, CCR4, and CCR6. CD32 expression did not selectively enrich for HIV- or SIV-infected CD4+ T cells in peripheral blood or lymphoid tissue; isolated CD32+ resting CD4+ T cells accounted for less than 3% of the total HIV DNA in CD4+ T cells. Cell-associated HIV DNA and RNA loads in CD4+ T cells positively correlated with the frequency of CD32+ CD69+ CD4+ T cells but not with CD32 expression on resting CD4+ T cells. Using RNA fluorescence in situ hybridization, CD32 coexpression with HIV RNA or p24 was detected after in vitro HIV infection (peripheral blood mononuclear cell and tissue) and in vivo within lymph node tissue from HIV-infected individuals. Together, these results indicate that CD32 is not a marker of resting CD4+ T cells or of enriched HIV DNA–positive cells after ART; rather, CD32 is predominately expressed on a subset of activated CD4+ T cells enriched for transcriptionally active HIV after long-term ART