Tracking nanoplastics in freshwater microcosms and their impacts to aquatic organisms

In this work, we used palladium-doped polystyrene NPLs (PS-NPLs with a primary size of 286 ± 4 nm) with an irregular surface morphology which allowed for particle tracking and evaluation of their toxicity on two primary producers (cyanobacterium, Anabaena sp. PCC7120 and green algae, Chlamydomonas reinhardtii) and one primary consumer (crustacean, Daphnia magna). the concentration range for Anabaena and C. reinhardtii was from 0.01 to 1000 mg/L and for D. magna, the range was from 7.5 to 120 mg/L.EC50 s ranged from 49 mg NPLs/L for D. magna (48hEC50 s) to 248 mg NPLs/L (72hEC50 s for C. reinhardtii). PS-NPLs induced dose-dependent reactive oxygen species overproduction, membrane damage and metabolic alterations. To shed light on the environmental fate of PS-NPLs, the short-term distribution of PS-NPLs under static (using lake water and sediments) and stirring (using river water and sediments) conditions was studied at laboratory scale. The results showed that most NPLs remained in the water column over the course of 48 h. The maximum percentage of settled particles (∼ 30 %) was found under stirring conditions in comparison with the ∼ 10 % observed under static ones. Natural organic matter increased the stability of the NPLs under colloidal state while organisms favored their settlement. This study expands the current knowledge of the biological effects and fate of NPLs in freshwater environment

Microplastic pollution in sublittoral coastal sediments of a North Atlantic island: The case of La Palma (Canary Islands, Spain)

In this work, the microplastic content of sediments collected in July 2020 between 5 and 7 m depth was studied in four locations of La Palma island (Canary Islands, Spain). At each sampling location, three samples were taken parallel to the shoreline. The microplastic content in each sampling corer was studied every 2.5 cm depth after digestion with a H2O2 solution followed by flotation in a saturated NaCl solution. Visualization of the final filtrates under a stereomicroscope revealed that all the sediment samples evaluated contained mostly microfibers (98.3%) which were mainly white/colorless (86.0%) and blue (9.8%), with an average length of 2423 ± 2235 (SD) mm and an average concentration of 2682 ± 827 items per kg of dry weight, being the total number of items found 1,019. Fourier Transform Infrared microscopy analysis of 13.9% (n = 139) of the microfibers also showed that they were mainly cellulosic (81.3%). No significant differences were found between the depths of the sediment. However, significant differences were found between the number of fibers from the sampling sites at the east and west of the island. Such variability could be driven by the winds and ocean mesoscale dynamics in the area. This study confirms the wide distribution of microfibers in sediments from an oceanic island like La Palma, providing their first report in marine sediments of the Canary Islands.En prensa3,20

Probing Novel Scalar and Tensor Interactions from (Ultra)Cold Neutrons to the LHC

Scalar and tensor interactions were once competitors to the now well-established V-A structure of the Standard Model weak interactions. We revisit these interactions and survey constraints from low-energy probes (neutron, nuclear, and pion decays) as well as collider searches. Currently, the most stringent limit on scalar and tensor interactions arise from 0+ -> 0+ nuclear decays and the radiative pion decay pi -> e nu gamma, respectively. For the future, we find that upcoming neutron beta decay and LHC measurements will compete in setting the most stringent bounds. For neutron beta decay, we demonstrate the importance of lattice computations of the neutron-to-proton matrix elements to setting limits on these interactions, and provide the first lattice estimate of the scalar charge and a new average of existing results for the tensor charge. Data taken at the LHC is currently probing these interactions at the 10^-2 level (relative to the standard weak interactions), with the potential to reach the 10^-3 level. We show that, with some theoretical assumptions, the discovery of a charged spin-0 resonance decaying to an electron and missing energy implies a lower limit on the strength of scalar interactions probed at low energy.Comment: 58 pages, 14 figure

Analysis of norfloxacin ecotoxicity and the relation with its degradation by means of electrochemical oxidation using different anodes

[EN] In this work, ecotoxicological bioassays based on Lactuca sativa seeds and bioluminescent bacterium (Vibrio fischeri) have been carried out in order to quantify the toxicity of Norfloxacin (NOR) and sodium sulfate solutions, before and after treating them using electrochemical advanced oxidation. The effect of some process variables (anode material, reactor configuration and applied current) on the toxicity evolution of the treated solution has been studied. A NOR solution shows an EC50 (5 days) of 336 mg L-1 towards Lactuca sativa. This threshold NOR concentration decreases with sodium sulfate concentration, in solutions that contain simultaneously Norfloxacin and sodium sulfate. In every case considered in this work, the electrochemical advanced oxidation process increased the toxicity (towards both Lactuca sativa and Vibrio fischeri) of the solution. This toxicity increase is mainly due to the persulfate formation during the electrochemical treatment. From a final solution toxicity point of view, the best results were obtained using a BDD anode in a divided reactor applying the lowest current intensity.The authors are very grateful to the Ministerio de Economia y Competitividad (Projects CTQ2015-65202-C2-1-R and RTI2018-101341-B-C21) for their economic support.Montañés, M.; García Gabaldón, M.; Roca-Pérez, L.; Giner-Sanz, JJ.; Mora-Gómez, J.; Pérez-Herranz, V. (2020). Analysis of norfloxacin ecotoxicity and the relation with its degradation by means of electrochemical oxidation using different anodes. Ecotoxicology and Environmental Safety. 188:1-10. https://doi.org/10.1016/j.ecoenv.2019.109923S110188Banks, M. K., & Schultz, K. E. (2005). Comparison of Plants for Germination Toxicity Tests in Petroleum-Contaminated Soils. Water, Air, and Soil Pollution, 167(1-4), 211-219. doi:10.1007/s11270-005-8553-4Barreto, J. P. d. P., Araujo, K. C. d. F., de Araujo, D. M., & Martinez-Huitle, C. A. (2015). 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Intracellular free Ca2+ signals antibiotic exposure in cyanobacteria

Intracellular free Ca2+, [Ca2+]i, is a key element of the cellular response to many abiotic and biotic stresses. Cyanobacteria are ancient organisms which have evolved signal transduction systems in response to an ever changing environment. Cyanobacteria have been found to respond to specific and quick Ca2+ signatures (unique combinations of changes in [Ca2+]i) when exposed to environmental stress. Antibiotics are bioactive molecules which are being considered as pseudopersistent contaminants as they are being continuously released to the environment; although non-target organisms, cyanobacteria as prokaryotes are sensitive to antibiotics. In order to check whether [Ca2+]i could be involved in sensing antibiotic exposure, we have recorded the Ca2+ signatures induced by antibiotics belonging to different classes at two effective toxicity concentrations, the EC10 and the EC50. For that, we have used a recombinant bioluminescent strain of the cyanobacterium Anabaena sp. PCC7120 that constitutively expresses the calcium-binding indicator protein, apoaequorin. We found that Ca2+ signatures upon exposure to antibiotics were induced quickly (2-3 seconds) and that they differed between antibiotics and antibiotic concentration. As different classes of antibiotics have been detected simultaneously in the aquatic environment, we also recorded and analyzed the Ca2+ signatures induced by binary mixtures and a multiantibiotic mixture; we found that these Ca2+ signatures could determine the nature of the interaction between antibiotics in the mixture, whether synergistic or antagonistic, well before toxicity was evident. To our knowledge, this study is the first report of intracellular free Ca2+ as an early biomarker of antibiotic exposure in the environmentThis research was supported by CTM2013-45775-C2-2-R and CGL2010-15675 grants from MINECO/FEDER EU. MGP holds an FPI grant from MINECO/FEDER E