Determinación de la actividad lactasica de kluyveromyces fragilis

Se produjeron las células de Kluyveromyces-fragiiis por fermentación en cochada a 29°C, en un medio de cultivo previamente estandarizado por Manosalva, H., Nieto, G. (2) y se retiraron del medio de fermentación al inicio de la fase estacionaria (14 h) para posteriormente permeabilizarlas. Se compararon dos agentes de permeabilización, alcohol isoamílicoy bromuro de cetiltrimetilaminio(CTAB) y se determinó la actividad lactásica empleando dos sustratos, ortonitrofenil-(3Dgalactopiranósido (ONPG) y lactosa. La permeabilización de las células empleando uno u otro agente aumentó la actividad 200 veces respecto a las células intactas. Se hicieron ensayos de inmovilización de Kluyveromycesfragilis, por el método de absorción sobre tres carbones activados y se determinó la actividad lactásica de los complejos carbón-célula, usando lactosa como sustrato. Se encontró que a mayor área superficial mayor actividad del complejo carbón-célula

development of measures of polyneuropathy impairment in hattr amyloidosis from nis to mnis 7

Abstract Hereditary transthyretin-mediated amyloidosis (hATTR amyloidosis) is a rare, life-threatening disease, caused by point mutations in the transthyretin gene. It is a heterogeneous, multisystem disease with rapidly progressing polyneuropathy (including sensory, motor, and autonomic impairments) and cardiac dysfunction. Measures used to assess polyneuropathy in other diseases have been tested as endpoints in hATTR amyloidosis clinical trials (i.e. Neuropathy Impairment Score [NIS], NIS-lower limb, and NIS + 7), yet the unique nature of the polyneuropathy in this disease has necessitated modifications to these scales. In particular, the heterogeneous impairment and the aggressive disease course have been key drivers in developing scales that better capture the disease burden and progression of polyneuropathy in hATTR amyloidosis. The modified NIS + 7 (mNIS + 7) scale was specifically designed to assess polyneuropathy impairment in patients with hATTR amyloidosis, and has been the primary endpoint in two recent, phase III studies in this disease. The mNIS + 7 uses highly standardized, quantitative, and referenced assessments to quantify decreased muscle weakness, muscle stretch reflexes, sensory loss, and autonomic impairment. Physicians using this scale in clinical trials should be specifically trained and monitored to minimize variability. This article discusses the different scales that have been/are being used to assess polyneuropathy in patients with hATTR amyloidosis, their correlation with other disease assessments, and reflects on how and why scales have evolved to the latest iteration of mNIS + 7

The dependence of oxygen and nitrogen abundances on stellar mass from the CALIFA survey

We analysed the optical spectra of HII regions extracted from a sample of 350 galaxies of the CALIFA survey. We calculated total O/H abundances and, for the first time, N/O ratios using the semi-empirical routine HII-CHI-mistry, which, according to P\'erez-Montero (2014), is consistent with the direct method and reduces the uncertainty in the O/H derivation using [NII] lines owing to the dispersion in the O/H-N/O relation. Then we performed linear fittings to the abundances as a function of the de-projected galactocentric distances. The analysis of the radial distribution both for O/H and N/O in the non-interacting galaxies reveals that both average slopes are negative, but a non-negligible fraction of objects have a flat or even a positive gradient (at least 10\% for O/H and 4\% for N/O). The slopes normalised to the effective radius appear to have a slight dependence on the total stellar mass and the morphological type, as late low-mass objects tend to have flatter slopes. No clear relation is found, however, to explain the presence of inverted gradients in this sample, and there is no dependence between the average slopes and the presence of a bar. The relation between the resulting O/H and N/O linear fittings at the effective radius is much tighter (correlation coefficient $\rho_s$ = 0.80) than between O/H and N/O slopes ($\rho_s$ = 0.39) or for O/H and N/O in the individual \hii\ regions ($\rho_s$ = 0.37). These O/H and N/O values at the effective radius also correlate very tightly (less than 0.03 dex of dispersion) with total luminosity and stellar mass. The relation with other integrated properties, such as star formation rate, colour, or morphology, can be understood only in light of the found relation with mass.Comment: Accepted for publication in A&A. 20 pages, 19 figure

Performance of the CMS Level-1 trigger during commissioning with cosmic ray muons and LHC beams

52 páginas, 25 figuras.-- Open Access.-- CMS Collaboration: et al.The CMS Level-1 trigger was used to select cosmic ray muons and LHC beam events during data-taking runs in 2008, and to estimate the level of detector noise. This paper describes the trigger components used, the algorithms that were executed, and the trigger synchronisation. Using data from extended cosmic ray runs, the muon, electron/photon, and jet triggers have been validated, and their performance evaluated. Efficiencies were found to be high, resolutions were found to be good, and rates as expected.Acknowledge support from: FMSR (Austria); FNRS and FWO (Belgium); CNPq, CAPES, FAPERJ, and FAPESP (Brazil); MES (Bulgaria); CERN; CAS, MoST, and NSFC (China); COLCIENCIAS (Colombia); MSES (Croatia); RPF (Cyprus); Academy of Sciences and NICPB (Estonia); Academy of Finland, ME, and HIP (Finland); CEA and CNRS/IN2P3 (France); BMBF, DFG, and HGF (Germany); GSRT (Greece); OTKA and NKTH (Hungary); DAE and DST (India); IPM (Iran); SFI (Ireland); INFN (Italy); NRF (Korea); LAS (Lithuania); CINVESTAV, CONACYT, SEP, and UASLP-FAI (Mexico); PAEC (Pakistan); SCSR (Poland); FCT (Portugal); JINR (Armenia, Belarus, Georgia, Ukraine, Uzbekistan); MST and MAE (Russia); MSTDS (Serbia); MICINN and CPAN (Spain); Swiss Funding Agencies (Switzerland); NSC (Taipei); TUBITAK and TAEK (Turkey); STFC (United Kingdom); DOE and NSF (USA). Individuals have received support from the Marie-Curie IEF program (European Union); the Leventis Foundation; the A. P. Sloan Foundation; and the Alexander von Humboldt Foundation.Peer reviewe

Commissioning and performance of the CMS silicon strip tracker with cosmic ray muons

46 páginas, 17 figuras, 8 tablas.-- Open Access.-- CMS Collaboration: et al.During autumn 2008, the Silicon Strip Tracker was operated with the full CMS experiment in a comprehensive test, in the presence of the 3.8 T magnetic field produced by the CMS superconducting solenoid. Cosmic ray muons were detected in the muon chambers and used to trigger the readout of all CMS sub-detectors. About 15 million events with a muon in the tracker were collected. The efficiency of hit and track reconstruction were measured to be higher than 99% and consistent with expectations from Monte Carlo simulation. This article details the commissioning and performance of the Silicon Strip Tracker with cosmic ray muons.Individuals have received support from the Marie-Curie IEF program (European Union); the Leventis Foundation; the A. P. Sloan Foundation; and the Alexander von Humboldt Foundation.Peer reviewe

Fine synchronization of the CMS muon drift-tube local trigger using cosmic rays

33 páginas, 12 figuras.-- Open Access.-- CMS Collaboration: et al.The CMS experiment uses self-triggering arrays of drift tubes in the barrel muon trigger to perform the identification of the correct bunch crossing. The identification is unique only if the trigger chain is correctly synchronized. In this paper, the synchronization performed during an extended cosmic ray run is described and the results are reported. The random arrival time of cosmic ray muons allowed several synchronization aspects to be studied and a simple method for the fine synchronization of the Drift Tube Local Trigger at LHC to be developed.Individuals have received support from the Marie-Curie IEF program (European Union); the Leventis Foundation; the A. P. Sloan Foundation; and the Alexander von Humboldt Foundation.Peer reviewe

Performance of CMS muon reconstruction in cosmic-ray events

48 páginas, 23 figuras, 3 tablas.-- Open Access.-- CMS Collaboration: et al.The performance of muon reconstruction in CMS is evaluated using a large data sample of cosmic-ray muons recorded in 2008. Efficiencies of various high-level trigger, identification, and reconstruction algorithms have been measured for a broad range of muon momenta, and were found to be in good agreement with expectations from Monte Carlo simulation. The relative momentum resolution for muons crossing the barrel part of the detector is better than 1% at 10 GeV/c and is about 8% at 500 GeV/c, the latter being only a factor of two worse than expected with ideal alignment conditions. Muon charge misassignment ranges from less than 0.01% at 10 GeV/c to about 1% at 500 GeV/c.Acknowledge support from:FMSR (Austria); FNRS and FWO (Belgium); CNPq, CAPES, FAPERJ, and FAPESP (Brazil); MES (Bulgaria); CERN; CAS, MoST, and NSFC (China); COLCIENCIAS (Colombia); MSES (Croatia); RPF (Cyprus); Academy of Sciences and NICPB (Estonia); Academy of Finland, ME, and HIP (Finland); CEA and CNRS/IN2P3 (France); BMBF, DFG, and HGF (Germany); GSRT (Greece); OTKA and NKTH (Hungary); DAE and DST (India); IPM (Iran); SFI (Ireland); INFN (Italy); NRF (Korea); LAS (Lithuania); CINVESTAV, CONACYT, SEP, and UASLP-FAI (Mexico); PAEC (Pakistan); SCSR (Poland); FCT (Portugal); JINR (Armenia, Belarus, Georgia, Ukraine, Uzbekistan); MST and MAE (Russia); MSTDS (Serbia); MICINN and CPAN (Spain); Swiss Funding Agencies (Switzerland); NSC (Taipei); TUBITAK and TAEK (Turkey); STFC (United Kingdom); DOE and NSF (USA). Individuals have received support from the Marie-Curie IEF program (European Union); the Leventis Foundation; the A. P. Sloan Foundation; and the Alexander von Humboldt Foundation.Peer reviewe

Alignment of the CMS muon system with cosmic-ray and beam-halo muons

43 páginas, 11 figuras, 6 tablas.-- Open Access.-- CMS Collaboration: et al.The CMS muon system has been aligned using cosmic-ray muons collected in 2008 and beam-halo muons from the 2008 LHC circulating beam tests. After alignment, the resolution of the most sensitive coordinate is 80 microns for the relative positions of superlayers in the same barrel chamber and 270 microns for the relative positions of endcap chambers in the same ring structure. The resolution on the position of the central barrel chambers relative to the tracker is comprised between two extreme estimates, 200 and 700 microns, provided by two complementary studies. With minor modifications, the alignment procedures can be applied using muons from LHC collisions, leading to additional significant improvements.Individuals have received support from the Marie-Curie IEF program (European Union); the Leventis Foundation; the A. P. Sloan Foundation; and the Alexander von Humboldt Foundation.Peer reviewe

Alignment of the CMS silicon tracker during commissioning with cosmic rays

42 páginas, 15 figuras, 3 tablas.-- Open Access.-- CMS Collaboration: et al.The CMS silicon tracker, consisting of 1440 silicon pixel and 15 148 silicon strip detector modules, has been aligned using more than three million cosmic ray charged particles, with additional information from optical surveys. The positions of the modules were determined with respect to cosmic ray trajectories to an average precision of 3–4 microns RMS in the barrel and 3–14 microns RMS in the endcap in the most sensitive coordinate. The results have been validated by several studies, including laser beam cross-checks, track fit self-consistency, track residuals in overlapping module regions, and track parameter resolution, and are compared with predictions obtained from simulation. Correlated systematic effects have been investigated. The track parameter resolutions obtained with this alignment are close to the design performance.Individuals have received support from the Marie-Curie IEF program (European Union); the Leventis Foundation; the A. P. Sloan Foundation; and the Alexander von Humboldt Foundation.Peer reviewe