Expressão de leucócitos na circulação sangüínea antes, durante e após miíase por Dermatobia hominis em ratos experimentalmente infectados

A expressão de leucócitos sangüínea foi investigada em ratos (Rattus norvegicus) experimentalmente infectados com larvas de Dermatobia hominis. As células foram contadas antes, durante, aos 6, 10, 15, 20 e 28 dias pós-infestação (dpi), e aos 7, 15, 30 e 60 dias pós-emergência das larvas dos hospedeiros. O total de leucócitos não apresentou marcante diferença entre todos os grupos de animais. Todavia, diferenças significantes foram observadas quanto ao parasitismo pelos estádios larvares, com nível crescente: L1, L2, controle e L3. Na comparação entre grupos: o número de leucócitos foi significativo pró-controle, -15, -20 ou -28 dpi do que aos 6 dpi; e pró-controle, -L2 ou -L3 do que para L1. Neutrófilos, eosinófilos e linfócitos (pequenos e grandes) foram também analisados. Em contraste, o número insuficiente de basófilos e monócitos não permitiram estudos estatísticos. Estes resultados estimulam a continuação dos estudos sobre a relação parasito-hospedeiro nas dermatobioses.Expression of circulating white blood cells was investigated in rats (Rattus norvegicus) experimentally infected with larvae of Dermatobia hominis, the human bot fly. Leucocytes were counted prior to infection (control group) as well as at 6, 10, 15, 20 and 28 days post-infection (dpi) and at 7, 15, 30 and 60 days post-larval emergence (dple). Total leucocyte numbers did not differ markedly among the groups. Significant differences were registered when values from control and animals harboring each larval stage of D. hominis were compared; with crescent rank: L1-, L2-, control and L3-infected groups. Leucocyte numbers were significantly higher in the control, 15, 20 or 28 dpi groups than in the 6 dpi animals. Higher counts were observed in control, L2- or L3-infected rats than L1-infected animals. Neutrophils, eosinophils and both large and small lymphocytes were also counted and analyzed. Basophils and monocytes were insufficient in number to permit statistical studies. These results stimulate the continuity of the studies about the host-parasite relationship in the dermatobiosis

A BODIPY derivative for selective fluorescent chemosensing of iron (III)

A BODIPY derivative functionalized with a phenyl group at the meso-position was synthesized and characterized through 1H NMR and UV–Vis absorption/emission spectroscopies. The compound showed an absorption band at 497 nm and a fluorescence band at 513 nm, with a ΦF = 0.68 in acetonitrile. The evaluation of the chemosensing ability of the BODIPY was investigated in the presence of several ions with environmental and biomedical relevance. A highly selective fluorimetric response was observed for Fe3+ through fluorescence quenching upon successive additions of this cation.The authors acknowledge Fundação para a Ciência e Tecnologia—FCT (Portugal) for funding through CQUM (UID/QUI/00686/2020) and a PhD grant to R.C.R. Gonçalves (SFRH/BD/05278/2020). The NMR spectrometer Bruker Avance III 400 is part of the National NMR Network and was purchased within the framework of the National Program for Scientific Re-equipment, contract REDE/1517/RMN/2005 with funds from POCI 2010 (FEDER) and FCT

Avaliação de biofertilizantes, extratos vegetais e diferentes substâncias alternativas no manejo de tripes em cebola em sistema orgânico.

Avaliou-se substâncias alternativas no manejo de tripes (Thrips tabaci Lind.), em cebola, cv, Crioula, no sistema orgânico. Os experimentos foram conduzidos na EPAGRI, Ituporanga (SC). Os períodos entre transplante e colheita foram de 11/09/1996 a 10/01/1997 e 13/08/1997 a 11/12/1997. O delineamento foi de blocos ao acaso com 8 tratamentos em 1996 e 12 tratamentos em 1997 e quatro repetições. Em 1996 os tratamentos incluíram o biofertilizante anaeróbico 50%, biofertilizante aeróbico 5%, sulfato de manganês 1%, extrato hidroalcoólico de própolis 0,2%, macerado de ervas (“fersoral”) 2% e 4%, extrato de fumo (Nicotiana tabacum) 2 L ha-1 + 1% detergente neutro, testemunha sem aplicação. Em 1997 os tratamentos incluíram o macerado de ervas (“fersoral”) 5% 10%, enxofre pó molhável 0,25% + extrato hidroalcoólico de própolis 0,2% + extrato de samambaia 3%, biofertilizante anaeróbico 50%, biofertilizante aeróbico 5%, extrato de losna (Artemisia verlotorum) 3%, extrato de timbó (Ateleia glazioviana) 0,5%, extrato de samambaia (Pteridium aquilinum) 10%, extrato de erva-de-santa-maria (Chenopodium ambrosioides) 10%, extrato de cinamomo (Melia azedarach) 10%,extrato de camomila (Matricaria chamomilla) 5%, testemunha sem aplicação. Para aplicação dos produtos empregou-se pulverizador de pressão constante a base de CO2. Os tratamentos não causaram redução significativa na incidência de tripes e aumentos significativos na produtividade

Rede De Apoio Social, Saúde Mental E Qualidade De Vida: Um Estudo Transversal Na Atenção Primária

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)The objective of this study was to identify the association between emotional distress and social support networks with quality of life in primary care patients. This was a cross-sectional study involving 1,466 patients in the cities of São Paulo and Rio de Janeiro, Brazil, in 2009/2010. The General Health Questionnaire, the Hospital Anxiety and Depression Scale and the brief version of the World Health Organization Quality of Life Instrument were used. The Social Support Network Index classified patients with the highest and lowest index as socially integrated or isolated. A bivariate analysis and four multiple linear regressions were conducted for each quality of life outcome. The means scores for the physical, psychological, social relations, and environment domains were, respectively, 64.7; 64.2; 68.5 and 49.1. In the multivariate analysis, the psychological domain was negatively associated with isolation, whereas the social relations and environment domains were positively associated with integration. Integration and isolation proved to be important factors for those in emotional distress as they minimize or maximize negative effects on quality of life. © 2016, Fundacao Oswaldo Cruz. All rights reserved.3212CNPq, Conselho Nacional de Desenvolvimento Científico e TecnológicoConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Biochemical parameters of silver catfish (Rhamdia quelen) after transport with eugenol or essential oil of Lippia alba added to the water

The transport of live fish is a routine practice in aquaculture and constitutes a considerable source of stress to the animals. The addition of anesthetic to the water used for fish transport can prevent or mitigate the deleterious effects of transport stress. This study investigated the effects of the addition of eugenol (EUG) (1.5 or 3.0 mu L L-1) and essential oil of Lippia alba (EOL) (10 or 20 mu L L-1) on metabolic parameters (glycogen, lactate and total protein levels) in liver and muscle, acetylcholinesterase activity (AChE) in muscle and brain, and the levels of protein carbonyl (PC), thiobarbituric acid reactive substances (TBARS) and nonprotein thiol groups (NPSH) and activity of glutathione-S-transferase in the liver of silver catfish (Rhamdia quelen; Quoy and Gaimard, 1824) transported for four hours in plastic bags (loading density of 169.2 g L-1). The addition of various concentrations of EUG (1.5 or 3.0 mu L L-1) and EOL (10 or 20 mu L L-1) to the transport water is advisable for the transportation of silver catfish, since both concentrations of these substances increased the levels of NPSH antioxidant and decreased the TBARS levels in the liver. In addition, the lower liver levels of glycogen and lactate in these groups and lower AChE activity in the brain (EOL 10 or 20 mu L L-1) compared to the control group indicate that the energetic metabolism and neurotransmission were lower after administration of anesthetics, contributing to the maintenance of homeostasis and sedation status.Fundacao de Amparo a Pesquisa do Estado do Rio Grande do Sul (FAPERGS/PRONEX) [10/0016-8]; Conselho Nacional de Pesquisa e Desenvolvimento Cientifico (CNPq) [470964/2009-0]; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES); CNPqinfo:eu-repo/semantics/publishedVersio

Suspected adverse drug reactions reported for Brazilian children: cross‐sectional study

To assess spontaneous reports of suspected adverse drug reactions in children aged 0-12 years from the Brazilian Health Regulatory Agency between 2008 and 2013.A cross-sectional study on suspected adverse drug reactions reports related to medicines and health products in children was carried out for a six-year period (2008-2013). Year of report, origin of report by Brazilian state, gender, age, suspected drug, adverse reaction description and seriousness were included in the analysis. The data obtained was compared to the number of pediatric beds in health services and to global data from the VigiBase (World Health Organization).A total of 3330 adverse drug reactions were reported in children in Brazil in the investigated period (54% were in boys). About 28% of suspected adverse drug reactions reports involved 0 to 1-year-old children. Almost 40% of reports came from the Southeast region. Approximately 60% were classified as serious events. There was death in 75 cases. Nearly 30% of deaths involved off-label use; 3875 medicines (465 active substances) were considered suspected drugs. Anti-infective (vancomycin, ceftriaxone, oxacillin, and amphotericin), nervous system (metamizole) and alimentary tract and metabolism medicines were more frequent in reports.The distribution of suspected adverse drug reactions reports by sex and age group corresponded to the profile of children hospitalized in Brazil. Data about seriousness and medicines reported may be useful to encourage regulatory actions and improve the safe use of medicines in children