The CreC regulator of Escherichia coli, a new target for metabolic manipulations

The CreBC (carbon source-responsive) two-component regulation system of Escherichia coli affects a number of functions, including intermediary carbon catabolism. The impacts of different creC mutations (a ΔcreC mutant and a mutant carrying the constitutive creC510 allele) on bacterial physiology were analyzed in glucose cultures under three oxygen availability conditions. Differences in the amounts of extracellular metabolites produced were observed in the null mutant compared to the wild-type strain and the mutant carrying creC510 and shown to be affected by oxygen availability. The ΔcreC strain secreted more formate, succinate, and acetate but less lactate under low aeration. These metabolic changes were associated with differences in AckA and LdhA activities, both of which were affected by CreC. Measurement of the NAD(P)H/NAD(P)+ ratios showed that the creC510 strain had a more reduced intracellular redox state, while the opposite was observed for the ΔcreC mutant, particularly under intermediate oxygen availability conditions, indicating that CreC affects redox balance. The null mutant formed more succinate than the wild-type strain under both low aeration and no aeration. Overexpression of the genes encoding phosphoenolpyruvate carboxylase from E. coli and a NADH-forming formate dehydrogenase from Candida boidinii in the ΔcreC mutant further increased the yield of succinate on glucose. Interestingly, the elimination of ackA and adhE did not significantly improve the production of succinate. The diverse metabolic effects of this regulator on the central biochemical network of E. coli make it a good candidate for metabolic-engineering manipulations to enhance the formation of bioproducts, such as succinate.Fil: Godoy, Manuel Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Nikel, Pablo Ivan. Consejo Superior de Investigaciones Científicas; EspañaFil: Cabrera Gomez, José Gregorio. Unviversidad de San Pablo; BrasilFil: Pettinari, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentin

Comentario sobre Avila-Serrano et al. “Variaciones sedimentarias y transporte litoral en Playa de la Victoria, Cádiz, España”

The article published by Avila-Serrano et al. (2009) presents an interesting analysis of the sediment dynamics at Victoria Beach based on a series of samples taken together with fortnightly topographic profiles between February and October 2004. The authors of this comment, directors of this beach’s regeneration and monitoring programs over the past 20 years, believe some observations are relevant. We aim to provide a series of constructive comments that complement Avila-Serrano et al.’s commendable study

Current Strategies to Inhibit High Affinity FcεRI-Mediated Signaling for the Treatment of Allergic Disease

Allergies and asthma are a major cause of chronic disease whose prevalence has been on the rise. Allergic disease including seasonal rhinitis, atopic dermatitis, urticaria, anaphylaxis, and asthma, are associated with activation of tissue-resident mast cells and circulating basophils. Although these cells can be activated in different ways, allergic reactions are normally associated with the crosslinking of the high affinity Fc receptor for Immunoglobulin E, FcεRI, with multivalent antigen. Inflammatory mediators released from cytoplasmic granules, or biosynthesized de novo, following FcεRI crosslinking induce immediate hypersensitivity reactions, including life-threatening anaphylaxis, and contribute to prolonged inflammation leading to chronic diseases like asthma. Thus, inappropriate or unregulated activation of mast cells and basophils through antigenic crosslinking of FcεRI can have deleterious, sometimes deadly, consequences. Accordingly, FcεRI has emerged as a viable target for the development of biologics that act to inhibit or attenuate the activation of mast cells and basophils. At the forefront of these strategies are (1) Anti-IgE monoclonal antibody, namely omalizumab, which has the secondary effect of reducing FcεRI surface expression, (2) Designed Ankyrin Repeat Proteins (DARPins), which take advantage of the most common structural motifs in nature involved in protein-protein interactions, to inhibit FcεRI-IgE interactions, and (3) Fusion proteins to co-aggregate FcεRI with the inhibitory FcγRIIb. This review presents the published research studies that support omalizumab, DARPins, and fusion proteins as, arguably, the three most currently viable strategies for inhibiting the expression and activation of the high affinity FcεRI on mast cells and basophils

Characterization of wind-blown sediment transport with height in a highly mobile dune (SW Spain)

The Valdevaqueros dune is located at one of the windiest points of Europe, where the frequent occurrence of strong easterly winds has generated a highly mobile dune. Several rotating cup anemometers in vertical array and a self-designed vertical sand trap, were placed to retain the drift sands at different heights over the surface in order to determine theoretical and actual sand transport rates in the Valdevaqueros dune system. General results show that 90% of the wind-blown sand is transported within the first 20cm above the dune crest surface. Theoretical transport rates based on different empirical formulae were 0.33 to 0.78 times the in-situ sand transport rate detected, which was 2.08·10-2kgm-1s-1 under moderate wind power (mean speed ranging from 8.4 to 17.9ms-1). Analysis of different statistical grain-size parameters helped to understand sand transport distribution at different heights

Molecular Line Emission from Accretion Disks Around YSOs

In this work we model the expected molecular emission from protoplanetary disks, modifying different physical parameters, such as dust grain size, mass accretion rate, viscosity, and disk radius, to obtain observational signatures in these sources. Having in mind possible future observations, we study correlations between physical parameters and observational characteristics. Our aim is to determine the kind of observations that will allow us to extract information about the physical parameters of disks. We also present prospects for molecular line observations of protoplanetary disks, using millimeter and submillimeter interferometers (e.g., SMA or ALMA), based on our results.Comment: 6 pages, 2 figures. Proceeding of the workshop "Magnetic fields and star formation: theory versus observation", Madrid (Spain), April 21 - 25, 200

Dense gas and exciting sources of the molecular outflow in the AFGL 437 star-forming region

We present Very Large Array (VLA) high resolution observations of the NH3(1,1) and NH3(2,2) molecular transitions towards the high mass star forming region AFGL 437. Our aim was to investigate if the poorly collimated CO molecular outflow previously detected in the region is the result of a projection effect, with no intrinsic bipolarity, as suggested by Gomez et al. We complemented our observations with radio continuum archived data from the VLA at 2 and 3.6 cm, and with unpublished public data at 450 {\mu}m taken with Submillimetre Common-User Bolometer Array at the James Clerk Maxwell Telescope. Ammonia emission was found mainly in three clumps located at the south and east of the position of the compact infrared cluster of AFGL 437, where the CO outflow seemed to have its origin. One of the NH3(1,1) clumps coincides with the maximum of NH3(2,2) and with a local peak of emission at 450 {\mu}m. A near infrared source (s11) is also found at that position. Our continuum map at 2 cm shows extended elongated emission associated with the infrared source AFGL 437W. This elongated morphology and its spectral index between 3.6 and 2 cm (\simeq 0.4) suggest the presence of a jet in AFGL 437W. We suggest that several molecular bipolar outflows may exist in the region. The observed CO outflow would be the superposition of those individual outflows, which would explain its low degree of collimation observed at larger scales.Comment: 9 pages, 5 figures, 3 tables, accepted by MNRA

How to set challenging goals and conduct fair evaluation in regional public health systems. Insights from Valencia and Tuscany Regions

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New design parameters for biparabolic beach profiles (SW Cadiz, Spain)

165 profiles of seventy-one beaches along the Gulf of Cadiz (SW, Spain) were studied to improve the formulation of the beach profile in tidal seas. Maritime climate, degree of energy exposure and size of the sand grains were taken into account to study the two sections of the biparabolic profile. The objective of the study was the determination of more accurate formulations of the design parameters for the equilibrium profile that involves tidal seas. These formulations were modelled and validated based on existing profiles to quantify the error existing between the real profile and the modelling. This comparative analysis was extended by considering the formulations proposed by other authors. The best results were obtained with the proposal presented herein