Thermal receptivity of free convective flow from a heated vertical surface: linear waves

Numerical techniques are used to study the receptivity to small-amplitude thermal disturbances of the boundary layer flow of air which is induced by a heated vertical flat plate. The fully elliptic nonlinear, time-dependent Navier–Stokes and energy equations are first solved to determine the steady state boundary-layer flow, while a linearised version of the same code is used to determine the stability characteristics. In particular we investigate (i) the ultimate fate of a localised thermal disturbance placed in the region near the leading edge and (ii) the effect of small-scale surface temperature oscillations as means of understanding the stability characteristics of the boundary layer. We show that there is a favoured frequency of excitation for the time-periodic disturbance which maximises the local response in terms of the local rate of heat transfer. However the magnitude of the favoured frequency depends on precisely how far from the leading edge the local response is measured. We also find that the instability is advective in nature and that the response of the boundary layer consists of a starting transient which eventually leaves the computational domain, leaving behind the large-time time-periodic asymptotic state. Our detailed numerical results are compared with those obtained using parallel flow theory

Advancing the Frontier of Peacekeeping Research

The impact of United Nations (UN) peacekeeping on conflict has received a sustained amount of attention in the empirical literature. The advent of new data on UN peacekeeping and new temporal units of analysis have enabled researchers to expand the frontiers of peacekeeping research and undertake a more nuanced examination of peacekeeping effectiveness. In this special section, a series of articles examine how UN peacekeeping affects different types of violence within conflicts and leads to different types of peaceful outcomes. Factors such as the cultural affinity between peacekeepers and local communities, the size of peacekeeping operations and the specific composition of UN forces are shown to be important variables associated with lower levels of casualties and violence and also a higher likelihood of mediation and timely peaceful settlements in civil wars. In the aggregate, these articles suggest that robust peacekeeping is associated with better outcomes in many stages of conflict

Neurodegeneration and Motor Dysfunction in Mice Lacking Cytosolic and Mitochondrial Aldehyde Dehydrogenases: Implications for Parkinson's Disease

Previous studies have reported elevated levels of biogenic aldehydes in the brains of patients with Parkinson's disease (PD). In the brain, aldehydes are primarily detoxified by aldehyde dehydrogenases (ALDH). Reduced ALDH1 expression in surviving midbrain dopamine neurons has been reported in brains of patients who died with PD. In addition, impaired complex I activity, which is well documented in PD, reduces the availability of the NAD+ co-factor required by multiple ALDH isoforms to catalyze the removal of biogenic aldehydes. We hypothesized that chronically decreased function of multiple aldehyde dehydrogenases consequent to exposure to environmental toxins and/or reduced ALDH expression, plays an important role in the pathophysiology of PD. To address this hypothesis, we generated mice null for Aldh1a1 and Aldh2, the two isoforms known to be expressed in substantia nigra dopamine neurons. Aldh1a1−/−×Aldh2−/− mice exhibited age-dependent deficits in motor performance assessed by gait analysis and by performance on an accelerating rotarod. Intraperitoneal administration of L-DOPA plus benserazide alleviated the deficits in motor performance. We observed a significant loss of neurons immunoreactive for tyrosine hydroxylase (TH) in the substantia nigra and a reduction of dopamine and metabolites in the striatum of Aldh1a1−/−×Aldh2−/− mice. We also observed significant increases in biogenic aldehydes reported to be neurotoxic, including 4-hydroxynonenal (4-HNE) and the aldehyde intermediate of dopamine metabolism, 3,4-dihydroxyphenylacetaldehyde (DOPAL). These results support the hypothesis that impaired detoxification of biogenic aldehydes may be important in the pathophysiology of PD and suggest that Aldh1a1−/−×Aldh2−/− mice may be a useful animal model of PD

Multi-transmission-line-beam interactive system

We construct here a Lagrangian field formulation for a system consisting of an electron beam interacting with a slow-wave structure modeled by a possibly non-uniform multiple transmission line (MTL). In the case of a single line we recover the linear model of a traveling wave tube (TWT) due to J.R. Pierce. Since a properly chosen MTL can approximate a real waveguide structure with any desired accuracy, the proposed model can be used in particular for design optimization. Furthermore, the Lagrangian formulation provides for: (i) a clear identification of the mathematical source of amplification, (ii) exact expressions for the conserved energy and its flux distributions obtained from the Noether theorem. In the case of uniform MTLs we carry out an exhaustive analysis of eigenmodes and find sharp conditions on the parameters of the system to provide for amplifying regimes

Direct transfer of viral and cellular proteins from varicella-zoster virus-infected non-neuronal cells to human axons

10.1371/journal.pone.0126081PLoS ONE105e012608

A surrogate-based approach for post-genomic partner identification

BACKGROUND: Modern drug discovery is concerned with identification and validation of novel protein targets from among the 30,000 genes or more postulated to be present in the human genome. While protein-protein interactions may be central to many disease indications, it has been difficult to identify new chemical entities capable of regulating these interactions as either agonists or antagonists. RESULTS: In this paper, we show that peptide complements (or surrogates) derived from highly diverse random phage display libraries can be used for the identification of the expected natural biological partners for protein and non-protein targets. Our examples include surrogates isolated against both an extracellular secreted protein (TNFβ) and intracellular disease related mRNAs. In each case, surrogates binding to these targets were obtained and found to contain partner information embedded in their amino acid sequences. Furthermore, this information was able to identify the correct biological partners from large human genome databases by rapid and integrated computer based searches. CONCLUSIONS: Modified versions of these surrogates should provide agents capable of modifying the activity of these targets and enable one to study their involvement in specific biological processes as a means of target validation for downstream drug discovery

PAR Proteins Regulate Microtubule Dynamics at the Cell Cortex in C. elegans

BACKGROUND: The PAR proteins are known to be localized asymmetrically in polarized C. elegans, Drosophila, and human cells and to participate in several cellular processes, including asymmetric cell division and spindle orientation. Although astral microtubules are known to play roles in these processes, their behavior during these events remains poorly understood. RESULTS: We have developed a method that makes it possible to examine the residence time of individual astral microtubules at the cell cortex of developing embryos. Using this method, we found that microtubules are more dynamic at the posterior cortex of the C. elegans embryo compared to the anterior cortex during spindle displacement. We further observed that this asymmetry depends on the PAR-3 protein and heterotrimeric G protein signaling, and that the PAR-2 protein affects microtubule dynamics by restricting PAR-3 activity to the anterior of the embryo. CONCLUSIONS: These results indicate that PAR proteins function to regulate microtubule dynamics at the cortex during microtubule-dependent cellular processes

Compactifications of Heterotic Theory on Non-Kahler Complex Manifolds: I

We study new compactifications of the SO(32) heterotic string theory on compact complex non-Kahler manifolds. These manifolds have many interesting features like fewer moduli, torsional constraints, vanishing Euler character and vanishing first Chern class, which make the four-dimensional theory phenomenologically attractive. We take a particular compact example studied earlier and determine various geometrical properties of it. In particular we calculate the warp factor and study the sigma model description of strings propagating on these backgrounds. The anomaly cancellation condition and enhanced gauge symmetry are shown to arise naturally in this framework, if one considers the effect of singularities carefully. We then give a detailed mathematical analysis of these manifolds and construct a large class of them. The existence of a holomorphic (3,0) form is important for the construction. We clarify some of the topological properties of these manifolds and evaluate the Betti numbers. We also determine the superpotential and argue that the radial modulus of these manifolds can actually be stabilized.Comment: 75 pages, Harvmac, no figures; v2: Some new results added, typos corrected and references updated. Final version to appear in JHE

High‐density magnetospheric He+ at the dayside magnetopause and its effect on magnetic reconnection

Observations from the Magnetospheric Multiscale (MMS) mission are used to quantify the maximum effect of magnetospheric H+ and He+ on dayside magnetopause reconnection. A data base of current-sheet crossings from the first 2 years of the MMS mission is used to identify magnetopause crossings with the highest He+ concentrations. While all of these magnetopause crossings exhibit evidence of plasmaspheric plume material, only half of the crossings are directly associated with plasmaspheric plumes. The He+ density varies dramatically within the magnetosphere adjacent to the magnetopause, with density variations of an order of magnitude on timescales as short as 10 s, the time resolution of the composition instrument on MMS. Plasma wave observations are used to determine the total electron density, and composition measurements are used to determine the mass density in the magnetosheath and magnetosphere. These mass densities are then used with the magnetic field observations to determine the theoretical reduction in the reconnection rate at the magnetopause. The presence of high-density plasmaspheric plume material at the magnetopause causes transient reductions in the reconnection rate of up to ∼40%.publishedVersio