10.1371/journal.pone.0126081PLoS ONE105e012608

Garini, Yuval

Gerber, Doron

Glick, Yair

Goldstein, Ronald S.

Grigoryan, Sergei S.

Kepten, Eldad

Kinchington, Paul R.

YANG IN HONG

Yee, Michael

PLoS ONE

PubMed

Varicella Zoster Virus (VZV), the alphaherpesvirus that causes varicella upon primary infection and Herpes zoster (shingles) following reactivation in latently infected neurons, is known to be fusogenic. It forms polynuclear syncytia in culture, in varicella skin lesions and in infected fetal human ganglia xenografted to mice. After axonal infection using VZV expressing green fluorescent protein (GFP) in compartmentalized microfluidic cultures there is diffuse filling of axons with GFP as well as punctate fluorescence corresponding to capsids. Use of viruses with fluorescent fusions to VZV proteins reveals that both proteins encoded by VZV genes and those of the infecting cell are transferred in bulk from infecting non-neuronal cells to axons. Similar transfer of protein to axons was observed following cell associated HSV1 infection. Fluorescence recovery after photobleaching (FRAP) experiments provide evidence that this transfer is by diffusion of proteins from the infecting cells into axons. Time-lapse movies and immunocytochemical experiments in co-cultures demonstrate that non-neuronal cells fuse with neuronal somata and proteins from both cell types are present in the syncytia formed. The fusogenic nature of VZV therefore may enable not only conventional entry of virions and capsids into axonal endings in the skin by classical entry mechanisms, but also by cytoplasmic fusion that permits viral protein transfer to neurons in bulk

Grigoryan, Sergei

Yee, Michael B.

Yang, In Hong

Name not available

Direct Transfer of Viral and Cellular Proteins from Varicella-Zoster Virus-Infected Non-Neuronal Cells to Human Axons

Grigoryan, S

Yee, MB

Glick, Y

Gerber, D

Kepten, E

Garini, Y

Yang, IH

Kinchington, PR

Goldstein, RS

D-Scholarship@Pitt

Direct transfer of viral and cellular proteins from varicella-zoster virus-infected non-neuronal cells to human axons

ScholarBank@NUS

Sergei Grigoryan

Michael B Yee

Yair Glick

Doron Gerber

Eldad Kepten

Yuval Garini

In Hong Yang

Paul R Kinchington

Ronald S Goldstein

Directory of Open Access Journals

Direct transfer of viral and cellular proteins from varicella-zoster virus-infected non-neuronal cells to human axons.

http://d-scholarship.pitt.edu/28478/1/journal.pone.0126081.PDF

Direct transfer of viral and cellular proteins from varicella-zoster virus-infected non-neuronal cells to human axons

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D-Scholarship@Pitt

ScholarBank@NUS

Directory of Open Access Journals

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