The effect of erythropoietin on healing of obstructive vs nonobstructive left colonic anastomosis: an experimental study

<p>Abstract</p> <p>Background</p> <p>Anastomotic leakage is an important problem following primary resection in the left colon and is even more prominent when obstruction is present. We aimed to evaluate the possible effects of erythropoietin on the healing of anastomosis under both obstructive and non-obstructive states.</p> <p>Methods</p> <p>Forty male Wistar albino rats were divided into four groups. In group I, two cm left colonic resection and primary anastomosis were done. In group II, left colon were completely ligated and 24 hours later animals were re-operated for segmental resection. The same procedures were performed for rats in group III and IV in respect to group I and II and, 500 IU/kg a day erythropoietin were given in the latter two groups for seven days. For the quantative description of anastomotic healing mechanical, biochemical and histopathological parameters were employed on the seventh day and the animals were sacrificied.</p> <p>Results</p> <p>Although erythropoietin had positive effects on bursting pressure in group IV when compared to group II, it has no effect in group III. Despite the increased tissue hydroxyproline levels in group IV, erythropoietin failed to show any effects in group III.</p> <p>Erythropoietin had positive effects on neovascularization, fibroblast proliferiation and storage of collagen in group IV.</p> <p>Conclusion</p> <p>We failed to find any direct and evident effects of erythropoietin on healing of left colonic anastomosis. On the other hand, erythropoietin might prevent negative effects of obstruction on healing.</p

An analysis of the conformation and electronic structure of newly synthesized cryptand

1013-1015Using molecular mechanical methods (MM2, AMBER, OPLS), a conformational analysis of a newly synthesized cryptand is made and its stable conformations are determined. The electronic structures of the stable conformations are analysed by the AM1 method, and its donor-acceptor characteristics are investigated

A quantum chemical investigation of electrophilic addition reaction of bromine to bicyclo[3.2.2]nona-6,8-diene

221-226Full geometric optimization of bicyclo[3.2.2]nona-6,8-diene (BND) has been done by semiempirical and ab initio methods and the structure of the molecule has also been investigated. The double bond (I) situated in the opposite direction of methylene group in END molecule is more exo pyramidalized than the other double bond (II). The electron density (qi,HOMO) of the double bond(I) in HOMO of the molecule is more than that of the (II) double bond. Exo and endo faces of exo pyramidalized double bonds of the molecule are not equal and electron density is higher in endo faces. The molecular complexes of BND with bromine have been investigated by AM1 method and their stable configurations determined. The reason for endo molecular complexes being more stable than exo is that the stability is caused by electronic and steric factors. Because of electronic factors, BND ... Br2(endo1) complex is more stable than BND Br2 ...(endo2). The endo-bridged bromonium cation(I) is relatively more stable than the endo-bridged cation(IV). Endo- facial stereoselectivity and regioselectivity should be observed in the addition of bromine to BND molecule. Endo-facial stereoselectivity is caused by electronic and steric effects, regioselectivity by electronic effects. The rearranged bromocarbonium cation(V) is the most stable among the cationic intermediates and the ionic addition reaction occurs via this cation

Infectious Complications of Induction Therapies in Kidney Transplantation.

Background: Cytomegalovirus (CMV) and BK virus (BKV) are post-transplant opportunistic viral infections that affect patientand graft survival. This study was designed to evaluate the risk of BKV nephropathy and CMV disease in kidneytransplant recipients who received induction therapy with ATG or basiliximab.Material/Methods: We retrospectively analyzed information on 257 adult patients who underwent kidney transplantation betweenJanuary 2007 and 2017. Patients were categorized into 3 groups according to the induction therapies. The primaryendpoint was the onset of CMV disease or biopsy-confirmed BKV nephropathy. The secondary endpointswere biopsy-proven rejection episodes, graft loss, loss to follow-up, and death.Results: We followed 257 patients for a median of 55.5 months. The incidence of CMV disease was significantly higherin the only ATG group compared to the group without induction treatment (p&lt;0.001). There was no significantdifference in the incidence of BKV nephropathy among groups (p&gt;0.05). The dosage of ATG (OR, 10.685; 95%CI, 1.343 5 to 85.009; P=0.025) was independent risk factor for death.Conclusions: This study demonstrated that a higher dosage of ATG in high-risk patients is associated with an increased riskof CMV disease and patient death, also, reducing the dosage may be a rational strategy for increasing graftand patient’s survival

CHARACTERISTICS AND SURVIVAL RESULTS OF PERITONEAL DIALYSIS PATIENTS SUFFERING FROM COVID-19 IN TURKEY: A MULTICENTER NATIONAL STUDY

INTRODUCTION: We aimed to study the characteristics of peritoneal dialysis (PD) patients with coronavirus disease-19 (COVID-19), determine the short-term mortality and other medical complications, and delineate the factors associated with COVID-19 outcome. METHODS: In this multicenter national study, we included PD patients with confirmed COVID-19 from 27 centers. The baseline demographic, clinical, laboratory, and radiological data and outcomes at the end of the first month were recorded. RESULTS: We enrolled 142 COVID-19 patients (median age: 52 years). 58.2% of patients had mild disease at diagnosis. Lung involvement was detected in 60.8% of patients. Eighty-three (58.4%) patients were hospitalized, 31 (21.8%) patients were admitted to intensive care unit and 24 needed mechanical ventilation. Fifteen (10.5%) patients were switched to hemodialysis and hemodiafiltration was performed for four (2.8%) patients. Persisting pulmonary symptoms (n = 27), lower respiratory system infection (n = 12), rehospitalization for any reason (n = 24), malnutrition (n = 6), hypervolemia (n = 13), peritonitis (n = 7), ultrafiltration failure (n = 7), and in PD modality change (n = 8) were reported in survivors. Twenty-six patients (18.31%) died in the first month of diagnosis. The non-survivor group was older, comorbidities were more prevalent. Fever, dyspnea, cough, serious-vital disease at presentation, bilateral pulmonary involvement, and pleural effusion were more frequent among non-survivors. Age (OR: 1.102; 95% CI: 1.032–1.117; p: 0.004), moderate-severe clinical disease at presentation (OR: 26.825; 95% CI: 4.578–157.172; p < 0.001), and baseline CRP (OR: 1.008; 95% CI; 1,000–1.016; p: 0.040) were associated with first-month mortality in multivariate analysis. DISCUSSION/CONCLUSIONS: Early mortality rate and medical complications are quite high in PD patients with COVID-19. Age, clinical severity of COVID-19, and baseline CRP level are the independent parameters associated with mortality

Determinants of mortality in a large group of hemodialysis patients hospitalized for COVID-19

Background: Maintenance hemodialysis (MHD) patients are at increased risk for coronavirus disease 2019 (COVID-19). The aim of this study was to describe clinical, laboratory, and radiologic characteristics and determinants of mortality in a large group of MHD patients hospitalized for COVID-19

Characteristics and outcomes of hospitalised older patients with chronic kidney disease and COVID-19: A multicenter nationwide controlled study

Objective Older adults with co-morbidities have been reported to be at higher risk for adverse outcomes of coronavirus disease 2019 (COVID-19). The characteristics of COVID-19 in older patients and its clinical outcomes in different kidney disease groups are not well known

Middle-term outcomes in renal transplant recipients with COVID-19: a national, multicenter, controlled study

Background In this study, we evaluated 3-month clinical outcomes of kidney transplant recipients (KTR) recovering from COVID-19 and compared them with a control group. Method The primary endpoint was death in the third month. Secondary endpoints were ongoing respiratory symptoms, need for home oxygen therapy, rehospitalization for any reason, lower respiratory tract infection, urinary tract infection, biopsy-proven acute rejection, venous/arterial thromboembolic event, cytomegalovirus (CMV) infection/disease and BK viruria/viremia at 3 months. Results A total of 944 KTR from 29 different centers were included in this study (523 patients in the COVID-19 group; 421 patients in the control group). The mean age was 46 +/- 12 years (interquartile range 37-55) and 532 (56.4%) of them were male. Total number of deaths was 8 [7 (1.3%) in COVID-19 group, 1 (0.2%) in control group; P = 0.082]. The proportion of patients with ongoing respiratory symptoms [43 (8.2%) versus 4 (1.0%); P Conclusion The prevalence of ongoing respiratory symptoms increased in the first 3 months post-COVID in KTRs who have recovered from COVID-19, but mortality was not significantly different