Analysis of SARS-CoV-2 infection associated cell entry proteins ACE2, CD147, PPIA, and PPIB in datasets from non SARS-CoV-2 infected neuroblastoma patients, as potential prognostic and infection biomarkers in neuroblastoma

SARS-CoV-2 viral contagion has given rise to a worldwide pandemic. Although most children experience minor symptoms from SARS-CoV-2 infection, some have severe complications including Multisystem Inflammatory Syndrome in Children. Neuroblastoma patients may be at higher risk of severe infection as treatment requires immunocompromising chemotherapy and SARS-CoV-2 has demonstrated tropism for nervous cells. To date, there is no sufficient epidemiological data on neuroblastoma patients with SARS-CoV-2. Therefore, we evaluated datasets of non-SARS-CoV-2 infected neuroblastoma patients to assess for key genes involved with SARS-CoV-2 infection as possible neuroblastoma prognostic and infection biomarkers. We hypothesized that ACE2, CD147, PPIA and PPIB, which are associated with viral-cell entry, are potential biomarkers for poor prognosis neuroblastoma and SARS-CoV-2 infection. We have analysed three publicly available neuroblastoma gene expression datasets to understand the specific molecular susceptibilities that high-risk neuroblastoma patients have to the virus. Gene Expression Omnibus (GEO) GSE49711 and GEO GSE62564 are the microarray and RNA-Seq data, respectively, from 498 neuroblastoma samples published as part of the Sequencing Quality Control initiative. TARGET, contains microarray data from 249 samples and is part of the Therapeutically Applicable Research to Generate Effective Treatments (TARGET) initiative. ACE2, CD147, PPIA and PPIB were identified through their involvement in both SARS-CoV-2 infection and cancer pathogenesis. In-depth statistical analysis using Kaplan-Meier, differential gene expression, and Cox multivariate regression analysis, demonstrated that overexpression of ACE2, CD147, PPIA and PPIB is significantly associated with poor-prognosis neuroblastoma samples. These results were seen in the presence of amplified MYCN, unfavourable tumour histology and in patients older than 18 months of age. Previously, we have shown that high levels of the nerve growth factor receptor NTRK1 together with low levels of the phosphatase PTPN6 and TP53 are associated with increased relapse-free survival of neuroblastoma patients. Interestingly, low levels of expression of ACE2, CD147, PPIA and PPIB are associated with this NTRK1-PTPN6-TP53 module, suggesting that low expression levels of these genes are associated with good prognosis. These findings have implications for clinical care and therapeutic treatment. The upregulation of ACE2, CD147, PPIA and PPIB in poor-prognosis neuroblastoma samples suggests that these patients may be at higher risk of severe SARS-CoV-2 infection. Importantly, our findings reveal ACE2, CD147, PPIA and PPIB as potential biomarkers and therapeutic targets for neuroblastoma

High-resolution ice-thickness mapping in South Greenland

Airborne radar sounding is difficult in South Greenland because of the presence of englacial water, which prevents the signal from reaching the bed. Data coverage remains suboptimal for traditional methods of ice-thickness and bed mapping that rely on geostatistical techniques, such as kriging, because important features are missing. Here we apply two alternative approaches of highresolution (̃300 m) ice-thickness mapping, that are based on the conservation of mass, to two regions of South Greenland: (1) Qooqqup Sermia and Kiattuut Sermiat, and (2) Ikertivaq. These two algorithms solve optimization problems, for which the conservation of mass is either enforced as a hard constraint, or as a soft constraint. For the first region, very few measurements are available but there is no gap in ice motion data, whereas for Ikertivaq, more ice-thickness measurements are available, but there are gaps in ice motion data. We show that mass-conservation algorithms can be used as validation tools for radar sounding. We also show that it is preferable to apply mass conservation as a hard constraint, rather than a soft constraint, as it better preserves elongated features, such as glacial valleys and ridges

Rapid ice discharge from southeast Greenland glaciers

This is the published version, also available here: http://dx.doi.org/10.1029/2004GL019474.[1] Interferometric synthetic-aperture radar (InSAR) observations of southeast Greenland glaciers acquired by the Earth Remote Sensing Satellites (ERS-1/2) in 1996 were combined with ice sounding radar data collected in the late 1990s to estimate a total discharge of 46 ± 3 km3 ice per year between 62°N and 66°N, which is significantly lower than a mass input of 29 ± 3 km3 ice per year calculated from a recent compilation of snow accumulation data. Further north, Helheim Glacier discharges 23 ± 1 km3/yr vs 30 ± 3 km3/yr accumulation; Kangerdlugssuaq Glacier discharges 29 ± 2 km3/yr vs 23 ± 2 km3/yr; and Daugaard-Jensen Glacier discharges 10.5 ± 0.6 km3/yr vs 10.5 ± 1 km3/yr. The mass balance of east Greenland glaciers is therefore dominated by the negative mass balance of southeast Greenland glaciers (−17 ± 4 km3/yr), equivalent to a sea level rise of 0.04 ± 0.01 mm/yr. Warmer and drier conditions cannot explain the imbalance which we attribute to long-term changes in ice dynamics

Radar Thickness Measurements over the Southern Part of the Greenland Ice Sheet

We performed ice thickness measurements over the southern part of the Greenland ice sheet during June and July 1993. We used an airborne coherent radar depth sounder for these measurements. The radar was operated from a NASA P-3 aircraft equipped with GPS receivers. Radar data were collected in conjunction with laser altimeter and microwave altimeter measurements of ice surface elevation. This report provides radio echograms and thickness profiles from data collected during 1993

A search in north Greenland for a new ice-core drill site

This is the published version. Copyright International Glaciological SocietyA new deep ice-core drilling site has been identified in north Greenland at 75.12 ° N, 42 .30 ° W, 316 km north-northwest (NNW) of the GRIP drill site on the summit of the ice sheet. The ice thickness here is 3085 m; the surface elevation is 2919 m. The North GRIP (NG RIP) site is identified so that ice of Eemian age (115- 130 ka BP, calendar years before present ) is located as far above bedrock as possible and so the thickness of the Eemian layer is as great as possible. An ice-flow model, similar to the one used to date the GRIP ice core, is used to simulate the flow along the NNW-trending ice ridge. Surface and bedrock elevations, surface accumulation-rate distribution and radio-echo sounding along the ridge have been used as model input. The surface accumulation rate drops from 0.23 mice equivalent year 1 at GRIP to 0.19 mice equivalent year- 1 50 km from GRIP. Over the following 300 km the accumulation is relatively constant, before it starts decreasing again further north. Ice thicknesses up to 3250 m bring the temperature of the basal ice up to the pressure-melting point 100- 250 km from GRIP. The NGRIP site is located 316 km from GRIP in a region where the bedrock is smooth and the accumulation rate is 0.19 m ice equivalent year 1 • The modeled basal ice here has always been a few degrees below the pressure-melting point. Internal radio-echo sounding horizons can be traceq between the GRIP and NGRIP sites, allowing us to date the ice down to 2300 m depth (52 ka BP ). An ice-flow model predicts that the Eemian-age ice will be located in the depth range 2710 - 2800 m, which is 285 m above the bedrock. This is 120 m further above the bedrock, and the thickness of the Eemian layer of ice is 20 m thicker, than at the GRIP ice-core ite

Компонентный состав фразеологических единиц, мотивирующих слова (на материале немецкого языка)

Статья из специализированного выпуска научного журнала "Культура народов Причерноморья", материалы которого объединены общей темой "Язык и Мир" и посвящены общим вопросам Языкознания и приурочены к 80-летию со дня рождения Николая Александровича Рудякова.Стаття із спеціалізованого випуску наукового журналу "Культура народов Причерноморья", матеріали якого поєднані загальною темою "Мова і Світ" і присвячені загальним питанням мовознавства і приурочені до 80-річчя з дня народження Миколи Олександровича Рудякова

Constraining the recent mass balance of Pine Island and Thwaites glaciers, West Antarctica, with airborne observations of snow accumulation

In Antarctica, uncertainties in mass input and output translate directly into uncertainty in glacier mass balance and thus in sea level impact. While remotely sensed observations of ice velocity and thickness over the major outlet glaciers have improved our understanding of ice loss to the ocean, snow accumulation over the vast Antarctic interior remains largely unmeasured. Here, we show that an airborne radar system, combined with ice-core glaciochemical analysis, provide the means necessary to measure the accumulation rate at the catchment-scale along the Amundsen Sea coast of West Antarctica. We used along-track radar-derived accumulation to generate a 1985–2009 average accumulation grid that resolves moderate- to large-scale features (>25 km) over the Pine Island–Thwaites glacier drainage system. Comparisons with estimates from atmospheric models and gridded climatologies generally show our results as having less accumulation in the lower-elevation coastal zone but greater accumulation in the interior. Ice discharge, measured over discrete time intervals between 1994 and 2012, combined with our catchment-wide accumulation rates provide an 18-year mass balance history for the sector. While Thwaites Glacier lost the most ice in the mid-1990s, Pine Island Glacier's losses increased substantially by 2006, overtaking Thwaites as the largest regional contributor to sea-level rise. The trend of increasing discharge for both glaciers, however, appears to have leveled off since 2008

Extensive Liquid Meltwater Storage in Firn Within the Greenland Ice Sheet

The accelerating loss of mass from the Greenland ice sheet is a major contribution to current sea level rise. Increased melt water runoff is responsible for half of Greenlands mass loss increase. Surface melt has been increasing in extent and intensity, setting a record for surface area melt and runoff in 2012. The mechanisms and timescales involved in allowing surface melt water to reach the ocean where it can contribute to sea level rise are poorly understood. The potential capacity to store this water in liquid or frozen form in the firn (multi-year snow layer) is significant, and could delay its sea-level contribution. Here we describe direct observation of water within a perennial firn aquifer persisting throughout the winter in the southern ice sheet,where snow accumulation and melt rates are high. This represents a previously unknown storagemode for water within the ice sheet. Ice cores, groundairborne radar and a regional climatemodel are used to estimate aquifer area (70 plue or minus 10 x 10(exp 3) square kilometers ) and water table depth (5-50 m). The perennial firn aquifer represents a new glacier facies to be considered 29 in future ice sheet mass 30 and energy budget calculations