Migration and Ethnobotanical Practices: The Case of Tifey Among Haitian Immigrants in Cuba

Ethnobotanical knowledge and practices are dynamic and they change as they are transferred and appropriated by people who are adapting to new surroundings and changing environments. Using tifey, a multispecies drink, as a case study, we discuss the changes that emigration brought about related to tifey, and the processes that determined these changes. Tifey is a Haitian drink prepared by soaking Artemisia absinthium and other plants in rum or aguardiente. It probably had its origin in the adoption of the absinthe-based liquor used by French settlers and troops during the colonial period. Haitians progressively added culturally relevant flavorings and medicinal plants to this drink, and differentiated its production and use for medicinal, medicinal food, ritual ( religious and social), and economic purposes. When Haitians migrated to Cuba, they brought tifey with them, but over the course of the twentieth century its use declined and its composition changed due to sociocultural factors such as the dissolution of Haitian settlements, and to ecological factors such as difficulty in cultivation and/or procurement of A. absinthium in the new environment

Electronic Rubric Grading: Establishing a Foundation for the Future

Many institutions of higher education measure learning outcomes through performance-based assessments or rubrics, resulting in the exploration of innovative methods to administer these types of assessments (Anglin, Anglin, Schumann & Kaliski, 2008). At Western University of Health Sciences – College of Dental Medicine, performance-based assessments have been transformed into interactive, electronic versions in which faculty graders use their computers or mobile devices to submit scored rubrics complete with feedback for the students. A major advantage of the software, ExamSoft, we utilize is the ability to link learning outcomes to assessments, resulting in generating robust reports that display longitudinal data for individual students and each cohort. A pilot test was launched in December 2014 and lasted through February 2015 in order to gather data and improve processes prior to fully transitioning to electronic rubrics for dental competency exams. The development, implementation, and launching of electronic rubric grading was challenging, yet produced numerous benefits for the dental program. Faculty, staff, and administration can generate more robust reports that measure students’ institutional and program learning outcomes. With this data, longitudinal reports are a means to track and measure student outcomes. The transition to electronic grading has also resulted in a streamlined process, efficient and smoother workflow, decrease in the likelihood of miscalculations due to human error, quicker turnaround for releasing grades and feedback to faculty members and students, and data to support recommended improvements to the curriculum. Our advancement in electronic grading ensures our place at the forefront of dental education, assessment technologies, and higher education

Bioinspired snapping-claw apparatus to study hydrodynamic cavitation effects on the corrosion of metallic samples

A creative low-cost and compact mechanical device that mimics the rapid closure of the pistol shrimp claw was used to conduct electrochemical experiments, in order to study the effects of hydrodynamic cavitation on the corrosion of aluminum and steel samples. Current-time curves show significant changes associated with local variations in dissolved O2 concentration, cavitation-induced erosion and changes in the nature of the surface corrosion products

Laser-Assisted Cryosurgery in ex vivo Mice Hepatic Tissue: Viability Assays Using Green Fluorescent Protein

An experimental investigation is carried out to develop a novel approach to cryosurgery, where laser heating counteracts tissue freezing to better confine damage to the targeted cancerous tissue within a lethal low-temperature isothermal boundary—an approach we refer to as laser-assisted cryosurgery (LAC). The advantage of this procedure relative to conventional cryosurgery assisted with urethral warmers or cryoheaters is that laser heating provides volumetric rather than superficial heating, which leads to deeper penetration, more homogeneous tissue protection and better demarcation of the destructive freezing effect to a well-defined targeted volume. Tissue viability assays are performed using green fluorescence protein (GFP) as a viability marker and correlated with temperature history after performing LAC procedures on ex vivo mice hepatic tissue. The limit for cell denaturation at the irradiated surface predicted by GFP analysis is further confirmed using reverse transcription polymerase chain reaction (RT-PCR). In addition, the correlation between GFP fluorescence and cell viability and loss of GFP fluorescence in non-viable cells has been tested and validated by histological analysis using a standard cell viability measuring method (hematoxylin and eosin staining). Analysis of our experimental measurements show that reproducible thermal gradients (of 236 °C/cm) and predictable tissue necrosis can be reliably produced by LAC without exceeding temperature thresholds for cell denaturation (of Tsurf ≈ 48 °C) beyond preset tissue boundaries (with resolution of 0.1 °C/mm). The results have shown the feasibility of controlling temperatures at specified tissue locations to prevent hyperthermal or freezing damage

Experimental and computational analyses reveal that environmental restrictions shape HIV-1 spread in 3D cultures

Here, using an integrative experimental and computational approach, Imle et al. show how cell motility and density affect HIV cell-associated transmission in a three-dimensional tissue-like culture system of CD4+ T cells and collagen, and how different collagen matrices restrict infection by cell-free virions

T-cell derived acetylcholine aids host defenses during enteric bacterial infection with Citrobacter rodentium.

The regulation of mucosal immune function is critical to host protection from enteric pathogens but is incompletely understood. The nervous system and the neurotransmitter acetylcholine play an integral part in host defense against enteric bacterial pathogens. Here we report that acetylcholine producing-T-cells, as a non-neuronal source of ACh, were recruited to the colon during infection with the mouse pathogen Citrobacter rodentium. These ChAT+ T-cells did not exclusively belong to one Th subset and were able to produce IFNγ, IL-17A and IL-22. To interrogate the possible protective effect of acetylcholine released from these cells during enteric infection, T-cells were rendered deficient in their ability to produce acetylcholine through a conditional gene knockout approach. Significantly increased C. rodentium burden was observed in the colon from conditional KO (cKO) compared to WT mice at 10 days post-infection. This increased bacterial burden in cKO mice was associated with increased expression of the cytokines IL-1β, IL-6, and TNFα, but without significant changes in T-cell and ILC associated IL-17A, IL-22, and IFNγ, or epithelial expression of antimicrobial peptides, compared to WT mice. Despite the increased expression of pro-inflammatory cytokines during C. rodentium infection, inducible nitric oxide synthase (Nos2) expression was significantly reduced in intestinal epithelial cells of ChAT T-cell cKO mice 10 days post-infection. Additionally, a cholinergic agonist enhanced IFNγ-induced Nos2 expression in intestinal epithelial cell in vitro. These findings demonstrated that acetylcholine, produced by specialized T-cells that are recruited during C. rodentium infection, are a key mediator in host-microbe interactions and mucosal defenses