Identifying the transporters of different flavonoids in plants

We recently identified a new component of flavonoid transport pathways in Arabidopsis. The MATE protein FFT (Flower Flavonoid Transporter) is primarily found in guard cells and seedling roots, and mutation of the transporter results in floral and growth phenotypes. The nature of FFT’s substrate requires further exploration but our data suggest that it is a kaempferol diglucoside. Here we discuss potential partner H+-ATPases and possible redundancy among the close homologues within the large Arabidopsis MATE family

Extended x-ray absorption fine structure study of porous GaSb formed by ion implantation

Porous GaSb has been formed by Ga ion implantation into crystalline GaSb substrates at either room temperature or −180 °C. The morphology has been characterized using scanning electron microscopy and the atomic structure was determined using extended x-ray absorption fine structure spectroscopy. Room-temperature implantation at low fluences leads to the formation of ∼20-nm voids though the material remains crystalline. Higher fluences cause the microstructure to evolve into a network of amorphous GaSb rods ∼15 nm in diameter. In contrast, implantation at −180 °C generates large, elongated voids but no rods. Upon exposure to air, the surface of the porous material is readily oxidized yielding Ga₂O₃ and metallic Sb precipitates, the latter resulting from the reduction of unstable Sb₂O₃. We consider and discuss the atomic-scale mechanisms potentially operative during the concurrent crystalline-to-amorphous and continuous-to-porous transformations

Contribution of Scalar Loops to the Three-Photon Decay of the Z

I corrected 3 mistakes from the first version: that were an omitted Feynman integration in the function f^3_{ij}, a factor of 2 in front of log f^3_{ij} in eq.2 and an overall factor of 2 in Fig.1 c). The final result is changed drastically. Doing an expansion in the Higgs mass I show that the matrix element is identically 0 in the order (MZ/MH)^2, which is due to gauge invariance. Left with an amplitude of the order (MZ/MH)^4 the final result is that the scalar contribution to this decay rate is several orders of magnitude smaller than those of the W boson and fermions.Comment: 6 pages, plain Tex, 1 figure available under request via fax or mail, OCIP/C-93-5, UQAM-PHE-93/0

Force-detected nuclear magnetic resonance: Recent advances and future challenges

We review recent efforts to detect small numbers of nuclear spins using magnetic resonance force microscopy. Magnetic resonance force microscopy (MRFM) is a scanning probe technique that relies on the mechanical measurement of the weak magnetic force between a microscopic magnet and the magnetic moments in a sample. Spurred by the recent progress in fabricating ultrasensitive force detectors, MRFM has rapidly improved its capability over the last decade. Today it boasts a spin sensitivity that surpasses conventional, inductive nuclear magnetic resonance detectors by about eight orders of magnitude. In this review we touch on the origins of this technique and focus on its recent application to nanoscale nuclear spin ensembles, in particular on the imaging of nanoscale objects with a three-dimensional (3D) spatial resolution better than 10 nm. We consider the experimental advances driving this work and highlight the underlying physical principles and limitations of the method. Finally, we discuss the challenges that must be met in order to advance the technique towards single nuclear spin sensitivity -- and perhaps -- to 3D microscopy of molecules with atomic resolution.Comment: 15 pages & 11 figure

Carbon in different phases ([CII], [CI], and CO) in infrared dark clouds: Cloud formation signatures and carbon gas fractions

Context: How do molecular clouds form out of the atomic phase? And what are the relative fractions of carbon in the ionized, atomic and molecular phase? These are questions at the heart of cloud and star formation. Methods: Using multiple observatories from Herschel and SOFIA to APEX and the IRAM 30m telescope, we mapped the ionized, atomic and molecular carbon ([CII]@1900GHz, [CI]@492GHz and C18O(2-1)@220GHz) at high spatial resolution (12"-25") in four young massive infrared dark clouds (IRDCs). Results: The three carbon phases were successfully mapped in all four regions, only in one source the [CII] line remained a non-detection. Both the molecular and atomic phases trace the dense structures well, with [CI] also tracing material at lower column densities. [CII] exhibits diverse morphologies in our sample, from compact to diffuse structures probing the cloud environment. In at least two out of the four regions, we find kinematic signatures strongly indicating that the dense gas filaments have formed out of a dynamically active and turbulent atomic/molecular cloud, potentially from converging gas flows. The atomic-to-molecular carbon gas mass ratios are low between 7% and 12% with the lowest values found toward the most quiescent region. In the three regions where [CII] is detected, its mass is always higher by a factor of a few than that of the atomic carbon. The ionized carbon emission depends as well on the radiation field, however, we also find strong [CII] emission in a region without significant external sources, indicating that other processes, e.g., energetic gas flows can contribute to the [CII] excitation as well.Comment: 15 pages, 18 figures, accepted by Astronomy & Astrophysics, a higher resolution version can be found at http://www.mpia.de/homes/beuther/papers.htm

TOPoS: I. Survey design and analysis of the first sample

The metal-weak tail of the metallicity distribution function (MDF) of the Galactic Halo stars contains crucial information on the formation mode of the first generation of stars. To determine this observationally, it is necessary to observe large numbers of extremely metal-poor stars. We present here the Turn-Off Primordial Stars survey (TOPoS) that is conducted as an ESO Large Programme at the VLT. This project has {four} main goals: (i) to understand the formation of low-mass stars in a low-metallicity gas: determine the metal-weak tail of the halo MDF below [M/H]=-3.5. In particular, we aim at determining the critical metallicity, that is the lowest metallicity sufficient for the formation of low-mass stars; (ii) to determine the relative abundance of the elements in extremely metal-poor stars, which are the signature of the massive first stars; (iii) to determine the trend of the lithium abundance at the time when the Galaxy formed; and (iv) to derive the fraction of C-enhanced extremely metal-poor stars with respect to normal extremely metal-poor stars. The large number of stars observed in the SDSS provides a good sample of candidates of stars at extremely low metallicity. Candidates with turn-off colours down to magnitude g=20 were selected from the low-resolution spectra of SDSS by means of an automated procedure. X-Shooter has the potential of performing the necessary follow-up spectroscopy, providing accurate metallicities and abundance ratios for several key elements for these stars. We here present the stellar parameters of the first set of stars. The nineteen stars range in iron abundance between -4.1 and -2.9 dex relative to the Sun. Two stars have a high radial velocity and, according to our estimate of their kinematics, appear to be marginally bound to the Galaxy and are possibly accreted from another galaxy.Comment: A&A accepted for publicatio

PCNA binding proteins in Drosophila melanogaster : the analysis of a conserved PCNA binding domain

The eukaryotic polymerase processivity factor, PCNA, interacts with cell cycle regulatory proteins such as p21^(WAF1/Cip1) and Gadd45, as well as with proteins involved in the mechanics of DNA repair and replication. A conserved PCNA-binding motif is found in a subset of PCNA-interacting proteins, including p21, suggesting that the regulation of these interactions is important for the co-ordination of DNA replication and repair. We have identified several classes of protein which bind to Drosophila PCNA. Two of these proteins contain the consensus PCNA-binding domain: one is the Dacapo protein, a Drosophila homologue of p21^(WAF1/Cip1), and the second is the transposase encoded by the Pogo DNA transposon. A conserved PCNA-binding domain is also present in a human relative of Pogo, named Tigger, suggesting that this domain has a functional role in this class of transposable element. This raises interesting possibilities for a novel method of transposition in which the transposase might be targeted to replicating DNA. Finally, we have investigated the use of this conserved PCNAbinding domain as a predictor of PCNA-binding capacity