A microscopy technique based on bio-impedance sensors

It is proposed a microscopy for cell culture applications based on impedance sensors. The imagined signals are measured with the Electrical Cell-Substrate Spectroscopy (ECIS) technique, by identifying the cell area. The proposed microscopy allows real-time monitoring inside the incubator, reducing the contamination risk by human manipulation. It requires specific circuits for impedance measurements, a two-dimensional sensor array (pixels), and employing electrical models to decode efficiently the measured signals. Analogue Hardware Description Language (AHDL) circuits for cell-microelectrode enables the use of geometrical and technological data into the system design flow. A study case with 8x8 sensor array is reported, illustrating the evolution and power of the proposed image acquisition.Junta de Andalucía P0-TIC-538

Design for testability of high-order OTA-C filters

Copyright © 2016 John Wiley & Sons, Ltd.A study of oscillation-based test for high-order Operational Transconductance Amplifier-C (OTA-C) filters is presented. The method is based on partition of a high-order filter into second-order filter functions. The opening Q-loop and adding positive feedback techniques are developed to convert the second-order filter section into a quadrature oscillator. These techniques are based on an open-loop configuration and an additional positive feedback configuration. Implementation of the two testability design methods for nth-order cascade, IFLF and leapfrog (LF) filters is presented, and the area overhead of the modified circuits is also discussed. The performances of the presented techniques are investigated. Fourth-order cascade, inverse follow-the-leader feedback (IFLF) and LF OTA-C filters were designed and simulated for analysis of fault coverage using the adding positive feedback method based on an analogue multiplexer. Simulation results show that the oscillation-based test method using positive feedback provides high fault coverage of around 97%, 96% and 95% for the cascade, IFLF and LF OTA-C filters, respectively. Copyright ÂPeer reviewe

Testing mixed-signal cores: a practical oscillation-based test in an analog macrocell

A formal set of design decisions can aid in using oscillation-based test (OBT) for analog subsystems in SoCs. The goal is to offer designers testing options that do not have significant area overhead, performance degradation, or test time. This work shows that OBT is a potential candidate for IP providers to use in combination with functional test techniques. We have shown how to modify the basic concept of OBT to come up with a practical method. Using our approach, designers can use OBT to pave the way for future developments in SoC testing, and it is simple to extend this idea to BIST.European Union 2635

On-chip evaluation of oscillation-based-test output signals for switched-capacitor circuits

This work presents a simple and low-cost method for on-chip evaluation of test signals coming from the application of the Oscillation-Based-Test (OBT) technique. This method extracts the main test signal features (amplitude, frequency and DC level) in the digital domain requiring just a very simple and robust circuitry. Experimental results obtained from an integrated chip demonstrate the feasibility of the approac

Data-Analytics Modeling of Electrical Impedance Measurements for Cell Culture Monitoring

High-throughput data analysis challenges in laboratory automation and lab-on-a-chip devices’ applications are continuously increasing. In cell culture monitoring, specifically, the electrical cell-substrate impedance sensing technique (ECIS), has been extensively used for a wide variety of applications. One of the main drawbacks of ECIS is the need for implementing complex electrical models to decode the electrical performance of the full system composed by the electrodes, medium, and cells. In this work we present a new approach for the analysis of data and the prediction of a specific biological parameter, the fill-factor of a cell culture, based on a polynomial regression, data-analytic model. The method was successfully applied to a specific ECIS circuit and two different cell cultures, N2A (a mouse neuroblastoma cell line) and myoblasts. The data-analytic modeling approach can be used in the decoding of electrical impedance measurements of different cell lines, provided a representative volume of data from the cell culture growth is available, sorting out the difficulties traditionally found in the implementation of electrical models. This can be of particular importance for the design of control algorithms for cell cultures in tissue engineering protocols, and labs-on-a-chip and wearable devices applicationsEspaña, Ministerio de Ciencia e Innovación y Universidades project RTI2018-093512-B-C2

Estrés cotidiano infantil y memoria auditiva inmediata en estudiantes de cuarto y quinto de primaria de una Institución Educativa - 2017

La presente investigación tiene como objetivo determinar la relación entre el estrés cotidiano infantil y la memoria auditiva inmediata en estudiantes de cuarto y quinto de primaria de la institución educativa N° 82105 Escuela Concertada Solaris – 2017. Siendo este trabajo de tipo descriptivo – correlacional, ya que busca medir la relación entre el estrés cotidiano infantil, a través del Inventario de Estrés Cotidiano infantil (IECI) y la Memoria Auditiva Inmediata, mediante el test de memoria auditiva inmediata (MAI), en una población de 99 estudiantes de cuarto y quinto de primaria, el coeficiente de correlación utilizado se obtuvo por la correlación de spearman. Los resultados obtenidos muestran que si existe correlación (r= 0,246), con un nivel de significancia (p = 0.014) menor al 5%, entre la variable estrés cotidiano infantil y la memoria auditiva inmediata; asimismo si existe correlación (r= 0,352), con un nivel de significancia de (p= 0.00) entre la variable estrés en el ámbito familiar (IECI) y la memoria auditiva inmediata, mientras que no se encontró correlación entre las escalas de estrés en el ámbito escolar (r = 0,128) y problemas de salud y psicosomáticos (r = 0,033) y la memoria auditiva inmediata. Se obtuvo que el 52% de la población estudiada presenta un nivel regular de estrés, el 19% de los estudiantes un nivel alto de estrés y el 28% presenta un nivel bajo de estrés. Finalmente el 46% de los estudiantes logro obtener un nivel regular de memoria, mientras que el 27% alcanzo un nivel bajo, y el 26% un nivel alto de memoria

A practical floating-gate Muller-C element using vMOS threshold gates

This paper presents the rationale for vMOS-based realizations of digital circuits when logic design techniques based on threshold logic gates are used. Some practical problems in the vMOS implementation of threshold gates have been identified and solved. The feasibility and versatility of the proposed technique as well as its potential as a low-cost design technique for CMOS technologies have been shown by experimental results from a multiple-input Muller C-element. The proposed new realization exhibits better performance related to delay and area and power consumption than the traditional logic implementation

The bio-oscillator: A circuit for cell-culture assays

A system for cell-culture real-time monitoring using an oscillation-based approach is proposed. The system transforms a cell culture under test into a suitable “biological” oscillator, without needing complex circuitry for excitation and measurement. The obtained oscillation parameters are directly related to biological test, owed to an empirically extracted cell–electrode electrical model. A discrete prototype is proposed and experimental results with living cell culture are presented, achieving the expected performances.Ministerio de Economía y Competitividad TEC2011-28302, TEC2013-46242-C3-1-

Towards Bio-impedance Based Labs: A Review

In this article, some of the main contributions to BI (Bio-Impedance) parameter-based systems for medical, biological and industrial fields, oriented to develop micro laboratory systems are summarized. These small systems are enabled by the development of new measurement techniques and systems (labs), based on the impedance as biomarker. The electrical properties of the life mater allow the straightforward, low cost and usually non-invasive measurement methods to define its status or value, with the possibility to know its time evolution. This work proposes a review of bio-impedance based methods being employed to develop new LoC (Lab-on-a-Chips) systems, and some open problems identified as main research challenges, such as, the accuracy limits of measurements techniques, the role of the microelectrode-biological impedance modeling in measurements and system portability specifications demanded for many applications.Spanish founded Project: TEC 2013-46242-C3-1-P: Integrated Microsystem for Cell Culture AssaysFEDE

Cell-Culture Measurements Using Voltage Oscillations

A comprehensive system for real-time monitoring of a set of cell-cultures using a Voltage Oscillation (VO) methodology is proposed. The main idea is to connect the bio-electrical elements (electrodes & cell-culture) in such a way that sequentially a suitable electrical oscillator, which only uses a DC power source, is built. Using the employed electrical models given in [1, 2], the attained oscillation parameters (frequency and amplitude) can be directly related to the biological test. A digital circuitry is developed to pick-up the experimental measurements, which are gathered and shown in real-time in a web application.Ministerio de Economía y Competitividad TEC2013-46242-C3-1-