CYTOMORPHOLOGY OF THE BULBAR CONJUNCTIVAL CELLS IN PATIENTS WITH DRY EYE

Dry eye is among the most common pathological conditions in ophthalmology. The aim of our study was to present possibilites of two different cytological methods for examination of cytomorphology of bulbar conjunctival cells- impression cytology (IC) and combined cytological method for scanning electron microscopy in the diagnosis  of dry eye (ICSEM). A hundred and twenty-two patients of both sexes, in different age groups, were analyzed by clinical method (slit lamp, Schirmer I, TBUT, Rose Bengal) and two cytomorphological methods – IC and ICSEM. In patients with dry eye, squamous metaplasia, inflammation and severe loss of adhesiveness of the epithelium were present. ICSEM gives an advantage in early diagnosis of changes, before the lesion of superficial conjunctival epithelium in patients with dry eye. The phenomenon of metaplasia appears in the epithelium of the bulbar conjunctiva in the absence of manifest dry eye and represents the basis for understanding the increased incidence of this syndrome in older patients with dry eye

REAKTIVIRANJE BROWNFIELD-A U SRBIJI

Ulaganja stranih investitora u brownfield lokacijepodrazumevaju ulaganja u zemljiste i objekte koji su, danas, napusteni ili se minimalno koriste, cesto izazivajuci zagadenja zivotne sredine. Ove lokacije nalaze se u nekada razvi- jenim lokacijama u gradskim, industrijskim, prigradskim ili ruralnim podru- cjima. Revitalizacija, odnosno ponovno ozivljavanje brownfield lokacija ima pozitivne efekte na mnogim nivoima. Naime, ulaganje investicija u brownfield lokacije doprinosi povecanju ekonomskog razvoja, kroz porast zaposlenosti, investicija i revitalizaciju naselja. Ponovno ozivljavanje brownfield lokacija ima i fiskalni uticaj, koji ukljucuje generisanje novih izvora prihoda lokalne samouprave izvedene kroz strana ulaganja u neproduktivna zemljista i sma- njenje neophodnih ulaganja u infrastrukturu. Osim toga, ulaganje sredstava, kroz brownfield investicije ima povoljan uticaj i na zivotnu sredinu, cuvajuci zemljiste od negativnih spoljnih uticaja, smanjenje emisijegasova, poboljsanja kvaliteta vode i slicno.Stimulisanjem stranih investitora da ulazu u brownfield lokacije, gde vec postoji tehnicka i saobracajna infrastuktura postala bi velika sansa Republike Srbije, kojom bi se omogucio brzi i stabilniji ekonomski rast sa ciljem daljeg ukljucivanja zemlje u medunarodne integracione procese

Lomovi kondenzatorskih cevi od mesinga

Causes of failure of brass condenser tubes (CuZn28Sn1) in a thermal power plant in Serbia are analysed. Results of testing by Fourier transformed infrared spectroscopy (FTIR) have indicated the presence of ammonia and nitro- gen compounds on fracture surfaces and on the inner surface of the tubes. These compounds are specific agents that cause stress corrosion cracking (SCC) in brass. Analy- sis of fracture surfaces using scanning electron microscopy (SEM) has shown the brittle transgranular fracture due to the occurrence of SCC. The resistance of brass condenser tubes to other types of corrosion, such as erosion, general and selective corrosion (dezincification) is also considered. Some procedures are proposed to reduce the risk of SCC in the brass condenser tubes.Analizirani su uzroci pojave loma kondenzatorskih cevi od mesinga CuZn28Sn1 u jednoj termoelektrani u Srbiji. Rezultati ispitivanja metodom Furijeove transformisane infracrvene spektroskopije (FTIR) ukazuju na prisustvo amonijačnih i azotnih jedinjenja na površini loma i na unutrašnjoj površini cevi. Ta jedinjenja su specifični agensi za pojavu naponske korozije mesinga. Analiza površine loma primenom skening elektronske mikroskopije (SEM) pokazala je prisustvo krtog transkistalnog loma nastalog usled pojave naponske korozije. Razmatrana je otpornost cevi od mesinga CuZn28Sn1 prema drugim vidovima koro- zije, kao što su eroziona, opšta i selektivna korozija (decin- kacija). Predloženi su postupci za smanjenje rizika od pojave naponske korozije u kondenzatorskim cevima

Denture base resins biocompatibility testing in vivo

Background/Aim. The wearing of acrylic dentures is the cause of the inflammatory reaction of the oral mucosa. The aim of this study was to investigate the response of rat tissues to subcutaneous and intramuscular implantation of different acrylic samples, by histopathological analysis of the tissue. Methods. The study included two samples of hard and three samples of soft acrylic resins (heat and cold polymerized), that were subcutaneously and intramuscularly implanted in rats tissues. Implantation tests were designed to test the biological response of the surrounding tissue to the tested materials after their application for the period of two weeks and the period of four months. Results. After two weeks, regardless of the type of implantation, histopathological analysis showed an acute inflammatory response. There was an intense hyperplasia of inflammatory cells, multiplication of connective tissue as well as formation of many new blood vessels. The highest level of inflammatory changes was observed after the application of cold-polymerized resins. A lower intensity of inflammation in the case of heat polymerised resin was the result of its more complete polymerization. After the second observation period, fibrotic capsules were formed around the implanted samples indicating a chronic course of the inflammatory process. Less visible signs of inflammation and chronicity of the processes indicate that with time, i.e. with the length of the observation period, reduces inflammation. Conclusion. The subcutaneous and intramuscular implantation of acrylic resins material samples led to inflammatory response whose intensity was decreased over time. Heat polymerized resin was a biologically more acceptable in comparison to the cold polymerized acrylates

Mesenteric cyst

Mesenteric cysts are rare abdominal findings. Due to absent or unspecific clinical presentation, very low incidence, and lack of adequate classification these cysts may sometimes represent a diagnostic and therapeutic challenge. We report a case of 37-year-old man with vague palpatory tenderness in left hypochondrium and paraumbilically and with palpable large intra-abdominal mass in whom mesenteric cyst was diagnosed using US and CT imaging. He was operated and cyst was extirpated in toto. Histopathological examination revealed a thick fibrous cyst wall with the signs of chronic inflammation and without inner epithelial lining, which suggested its traumatic origin. Considering the possibility of malignancy mesenteric cysts should be radically resected (with resection of adjacent organs if necessary) due to their strong relapsing potential and a tendency for sudden, progressive local enlargement if not removed in toto

Failures of brass condenser tubes

Causes of failure of brass condenser tubes (CuZn28Sn1) in a thermal power plant in Serbia are analysed. Results of testing by Fourier transformed infrared spectroscopy (FTIR) have indicated the presence of ammonia and nitrogen compounds on fracture surfaces and on the inner surface of the tubes. These compounds are specific agents that cause stress corrosion cracking (SCC) in brass. Analysis of fracture surfaces using scanning electron microscopy (SEM) has shown the brittle transgranular fracture due to the occurrence of SCC. The resistance of brass condenser tubes to other types of corrosion, such as erosion, general and selective corrosion (dezincification) is also considered. Some procedures are proposed to reduce the risk of SCC in the brass condenser tubes

Antimicrobial synergism and cytotoxic properties of Citrus limon L., Piper nigrum L. and Melaleuca alternifolia (Maiden and Betche) Cheel essential oils

Objectives: The chemical composition, antimicrobial and synergistic effect, and cytotoxic activity of Citrus limon (lemon), Piper nigrum (green pepper) and Melaleuca alternifoila (tea tree) essential oils (EOs) were investigated. Methods: Chemical analyses of essential oils were tested by GC-FID and GC-MS spectroscopy. The antimicrobial activity assay was conducted using microdilution method against several oral bacteria and Candida spp. originating from the humans with oral disorders. The synergistic antimicrobial activity was evaluated using checkerboard method. The cytotoxicity evaluation of EOs was assessed using MTT test. Key findings: Limonene (37.5%) and β-pinene (17.9%) were the major compounds in C. limon oil, β-pinene (34.4%), δ-3-carene (19.7%), limonene (18.7%) and α-pinene (10.4%) in P. nigrum oil and terpinen-4-ol (38.6%) and γ-terpinene (21.7%) in M. alternifolia oil. The broad-spectrum antimicrobial activity was achieved by tested three EOs, with C. limon oil being the strongest against bacteria and M. alternifolia oil strongest against fungi. The EOs demonstrated synergism; their combined application revealed an increase in antimicrobial activity. All tested essential oils showed lower cytotoxic activity in comparison with the positive control, and the obtained results confirmed a dose-dependent activity. Conclusions: The results of this study encourage use of tested EOs in development of a novel agent intended for prevention or therapy of corresponding oral disorders.Journal of Pharmacy and Pharmacology (2017), 69(11): 1606-161