Quantitative RT-PCR assay for VEGF mRNA in human tumors of the kidney.

8Angiogenesis is the formation of new capillaries from pre-existing vessels, and recent evidence has demonstrated that tumor growth is controlled mainly by angiogenesis. Vascular endothelial growth factor (VEGF) is an endothelium-specific growth factor which is strongly angiogenic in vitro and in vivo. We have developed a quantitative RT-PCR assay for the measurement of VEGF mRNA expression using a real-time procedure based on the use of fluorogenic probes and the ABI PRISM 7700 Sequence Detector System. The assay performance of this method in terms of practicability and reliability is reported with results that seem promising for its widespread use in the clinical laboratory. The method has been applied to the measurement of mRNA of VEGF in human renal cell carcinomas (RCC). Preliminary results show a significantly higher VEGF mRNA expression (ratio values between 181 and 2222) in tumor specimens compared to non-adjacent, non-tumoral tissue of the same subjects.nonenoneC. Tricarico;B. Salvadori;D. Villari;G. Nicita;A. D. Melina;P. Pinzani; M. Ziche; M. PazzagliC., Tricarico; B., Salvadori; D., Villari; G., Nicita; A. D., Melina; P., Pinzani; Ziche, Marina; M., Pazzagl

Prostate Cancer Characteristics in Renal Transplant Recipients: A 25-Year Experience From a Single Centre

Gallic acid and catechin are the most abundant phenolic and flavonoid contents found in all plant extracts. The contents and the bioassay-guided fractionating substances of th