Single-born XX /XY chimaeric bulls with normal phenotype

International audienc

„Rendering Endangered Lexicons Interoperable through Standards Harmonization”: The RELISH Project

The RELISH project promotes language-oriented research by addressing a two-pronged problem: (1) the lack of harmonization between digital standards for lexical information in Europe and America, and (2) the lack of interoperability among existing lexicons of endangered languages, in particular those created with the Shoebox/Toolbox lexicon building software. The cooperation partners in the RELISH project are the University of Frankfurt (FRA), the Max Planck Institute for Psycholinguistics (MPI Nijmegen), and Eastern Michigan University, the host of the Linguist List (ILIT). The project aims at harmonizing key European and American digital standards whose divergence has hitherto impeded international collaboration on language technology for resource creation and analysis, as well as web services for archive access. Focusing on several lexicons of endangered languages, the project will establish a unified way of referencing lexicon structure and linguistic concepts, and develop a procedure for migrating these heterogeneous lexicons to a standards-compliant format. Once developed, the procedure will be generalizable to the large store of lexical resources involved in the LEGO and DoBeS projects

The sum of its parts? Sources of local legitimacy

The article analyses the sources of local actors’ legitimacy perceptions towards international peacebuilding operations. Local legitimacy perceptions are increasingly recognised as shaping local behaviour towards international peacebuilding, which influences the effective functioning of the operation. Legitimacy debates in peacebuilding are either absent or imported from the literature on domestic legitimacy, without respect to the specific temporal and spatial situation of international operations. The article first explores which legitimacy sources influence local legitimacy perceptions of international peacebuilding operations. It finds that two sources are relevant: output and procedure. Second, it investigates how exactly legitimacy arises from them. In doing so, it demonstrates that output and procedure are umbrella terms comprising several sub-elements which influence legitimacy in different, sometimes contradictory, ways. Finally, the article empirically explores which of the sources are important to local actors’ legitimacy perceptions using field data from the EU peacebuilding operations EULEX in Kosovo and EUPM Bosnia-Herzegovina

The C-Type Lysozyme from the upper Gastrointestinal Tract of Opisthocomus hoatzin, the Stinkbird

Muramidases/lysozymes are important bio-molecules, which cleave the glycan backbone in the peptidoglycan polymer found in bacterial cell walls. The glycoside hydrolase (GH) family 22 C-type lysozyme, from the folivorous bird Opisthocomus hoazin (stinkbird), was expressed in Aspergillus oryzae, and a set of variants was produced. All variants were enzymatically active, including those designed to probe key differences between the Hoatzin enzyme and Hen Egg White lysozyme. Four variants showed improved thermostability at pH 4.7, compared to the wild type. The X-ray structure of the enzyme was determined in the apo form and in complex with chitin oligomers. Bioinformatic analysis of avian GH22 amino acid sequences showed that they separate out into three distinct subgroups (chicken-like birds, sea birds and other birds). The Hoatzin is found in the “other birds” group and we propose that this represents a new cluster of avian upper-gut enzymes

The development of the würde + infinitive construction in Early Modern German (1650–1800)

This paper presents a corpus-based analysis of the evolution of the würde + infinitive construction in German during the Early Modern period (1650– 1800), using newly available data from the GerManC-corpus. We demonstrate how this construction occupies a unique position orthogonal to both the tense and mood systems of German through an analysis of the syntax and semantics of würde + infinitive clauses, beginning with Modern Standard German and then subsequently with a historical focus on the late seventeenth and eighteenth centuries. Through both quantitative and qualitative analyses, we examine the contexts in which the construction occurs and see how, as it came to be used more frequently over the period in question, it encroached more and more into contexts which had been the preserve of the synthetic preterite subjunctive, even being used in some where the latter is still the norm in modern German. Thus, by the end of the eighteenth century it had become difficult to identify a clear difference in meaning and use between these forms, and the reasons why the würde + in- finitive construction may be preferred over the synthetic preterite subjunctive are by no means clear. We conclude our discussion with an overview of how the würde + infinitive construction was received in the prescriptive tradition during this key period in the standardization of German, seeing its stigmatization in some contexts in part as an attempt by prescriptive grammarians to establish an explicit and clearly justified role for it in the language

Fungal GH25 muramidases : New family members with applications in animal nutrition and a crystal structure at 0.78Å resolution

Muramidases/lysozymes hydrolyse the peptidoglycan component of the bacterial cell wall. They are found in many of the glycoside hydrolase (GH) families. Family GH25 contains muramidases/lysozymes, known as CH type lysozymes, as they were initially discovered in the Chalaropsis species of fungus. The characterized enzymes from GH25 exhibit both β-1,4-N-acetyl- and β-1,4-N,6-O-diacetylmuramidase activities, cleaving the β-1,4-glycosidic bond between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) moieties in the carbohydrate backbone of bacterial peptidoglycan. Here, a set of fungal GH25 muramidases were identified from a sequence search, cloned and expressed and screened for their ability to digest bacterial peptidoglycan, to be used in a commercial application in chicken feed. The screen identified the enzyme from Acremonium alcalophilum JCM 736 as a suitable candidate for this purpose and its relevant biochemical and biophysical and properties are described. We report the crystal structure of the A. alcalophilum enzyme at atomic, 0.78 Å resolution, together with that of its homologue from Trichobolus zukalii at 1.4 Å, and compare these with the structures of homologues. GH25 enzymes offer a new solution in animal feed applications such as for processing bacterial debris in the animal gut