HP-LT metamorphism in Elba Island: Implications for the geodynamic evolution of the inner Northern Apennines (Italy)

The inner Northern Apennines belt (i.e., northern Tyrrhenian Sea and Tuscany) is an Alpine chain affectedby high-P metamorphic conditions during its evolution. Although Elba Island is structurally located closeto the Adria-Europe suture zone, for several authors it represents a sector of the orogen affected bylow-P metamorphism. The involvement of Elba Island tectonic units in high-P metamorphism was onlysuspected for the sparse presence of phengitic white mica in the metasedimentary rocks. This paperpresents the first clear evidence of high-P and low-T metamorphism found in metabasite rocks embed-ded in the Cretaceous calcschist of eastern Elba Island. Mineral composition of metabasite includesGln + Cpx + Ep + Ab + Act + Qtz + Ilm ± Ti-oxide ± Spn and is indicative of a former equilibration in the epi-dote blueschist subfacies and subsequent retrogression in the greenschist facies. Recorded metamorphicconditions are P = 0.9–1.0 GPa and T = 330–350◦C. Tectonic discrimination using immobile elements inthe metabasite does not point to an oceanic setting. As a consequence, the metasedimentary succes-sion containing metabasite is explained as belonging to the Tuscan continental domain and not to theLigurian-Piedmont Ocean, as previously interpreted. Our results have two significant implications: (i)it is confirmed and strengthened that the tectonic stacking of the Elba Island units did not occur in alow-pressure context; (ii) Elba Island is now completely reconciled in the tectonic and metamorphicevolution of the inner Northern Apennines

PO-485 Low abundance circulating proteins in giant cell tumours of bone

Introduction Circulating low-abundance proteins/fragments generating from tumour cells and tissues, represent the most important source of cancer biomarkers useful for early diagnosis and prognosis. Giant cell tumour of bone (GCT) is a benign neoplasm occurring in the long bone and in the axial skeleton of young adults. Approximately 5% of GCT develop pulmonary metastases. Although many biomarkers have been proposed, identification of circulating low abundance molecules may be useful to predict metastasis with a non invasive method. Material and methods The hydrogel nanoparticles technique followed by mass spectrometry was used to detect low molecular weight serum proteins or protein fragments in serum of 20 GCT patients with different clinical course and in 10 healthy sera used as control. The most representative low-abundant de novo or differentially abundant proteins were submitted to String database in order to define protein-protein interaction network. Cluster analysis was performed to identify prognostic groups of patients with similar abundance of proteins that significantly discriminate between the groups. Results and discussions For the 25 low-abundant de novo or differentially abundant proteins identified, we recognised that the top interconnected pathways included protein activation cascade, wound healing, blood coagulation, cell-substrate adhesion. Proteoma cluster analysis separated metastasis-free from metastatic GCT patients in two well-defined groups where serum levels of signalling transduction mediators and regulators of kinase activity presented a high discriminatory power. Increased expression of proteins STAT5B, GRB2 and OXSR1 was related to a higher probability of metastasis. Conclusion In conclusion, using a no invasive technique, we identified differentially abundant serum biomarkers, also providing prognostic information in patients with GCT of bone. Future studies are ongoing to establish the interplay between these biomarkers in order to fully understand the mechanism involved in tumour development and to focus on the planning of tailored therapies that should be more effective and less toxic

Distribución del parásito <i>Lernaea cyprinacea</i> en el territorio argentino

Lernaea cyprinacea fue introducida en América, a principios del siglo XX, junto con peces tropicales de cultivo y/u ornamentales. Los efectos deletéreos sobre los hospedadores se identifican de forma sinèrgica, los propios de la actividad parasitaria y los causados por la colonización de agentes oportunistas. El presente trabajo pretende divulgar los hallazgos de L. cyprinacea en diferentes ambientes de Argentina.Facultad de Ciencias VeterinariasFacultad de Ciencias Naturales y Muse

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer‐reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state‐of‐the‐art handbook for basic and clinical researchers.DFG, 389687267, Kompartimentalisierung, Aufrechterhaltung und Reaktivierung humaner Gedächtnis-T-Lymphozyten aus Knochenmark und peripherem BlutDFG, 80750187, SFB 841: Leberentzündungen: Infektion, Immunregulation und KonsequenzenEC/H2020/800924/EU/International Cancer Research Fellowships - 2/iCARE-2DFG, 252623821, Die Rolle von follikulären T-Helferzellen in T-Helferzell-Differenzierung, Funktion und PlastizitätDFG, 390873048, EXC 2151: ImmunoSensation2 - the immune sensory syste