Relation between composition, antioxidant and antibacterial activities and botanical origin of multifloral bee pollen

Harvested bee pollen is valuable for its nutritional value and healthy properties. This work relates the botanical origin of sixteen bee pollens from Chile with their phenolic, protein and carotenoid content, and antioxidant/antibacterial activities. Our results showed that the chemical properties of different bee pollens are associated with the plant’ species from which each one was derived from. Some correlations between chemical properties and botanical origin were observed. Bee pollen showed between 20.0- 30.4% protein, 2.8-50.2 mg/kg carotenoids, 22.8-918.4 mg/kg phenolics, and 4.51-91.19 mmol Fe+2/kg pollen. Antibacterial activity was observed against all bacteria assayed even surpassing the activity of traditional antibiotics. Brassica sp. and Galega officinalis are an abundant source of antioxidants and antibacterial compounds. Other species such as those derived from fruit and endemic plants from Chile, although they occur less frequently, are also good source of these compounds. Some correlations between botanical origin and chemical, antioxidant and antibacterial properties were observed. Knowing the influence of plant species over the antioxidant and antibacterial properties of bee pollen, will allow selecting the best location for honeycombs and will allow beekeepers to differentiate and add value to their products

Literacia estatística, probabilística e financeira: caminhos que se cruzam

Nessa pesquisa qualitativa, de natureza bibliográfico-documental, nós abordamos diferentes concepções a respeito de três modalidades de literacia que temos investigado nos últimos anos: a Literacia Estatística, a Literacia Probabilística e a Literacia Financeira, em busca de possíveis intersecções entre essas três áreas da experiência humana. Consideramos esse tema de grande relevância para a educação brasileira, em tempos de reforma curricular, impulsionada pela publicação da Base Nacional Comum Curricular – BNCC, documento esse que confere grande importância ao multiletramento. A Probabilidade e a Estatística ganharam muito espaço a partir da publicação da BNCC, pois constituem uma unidade temática que deve ser explorada em sala de aula, desde a Educação Infantil até o término do Ensino Médio. Vale destacar, ainda, que a Educação Financeira, ausente nos documentos norteadores da educação nacional até então, os Parâmetros Curriculares Nacionais – PCN, agora aparece nos novos currículos com grande destaque, uma vez que adquiriu o status de tema transversal. Ao final, propomos um modelo de literacia financeira compatível com modelos de literacia estatística e probabilística amplamente utilizados na educação estatística

Ensino de Estatística, Probabilidade e Combinatória na Educação Básica: : os novos desafios da BNCC

This article aims to identify some of the main challenges for the teaching of Stochastics — Statistics, Probability and Combinatorics — in Brazilian Basic Education (elementary and high school), which serves students aged from six to seventeen years, from the curricular reform, triggered by the publication of the National Common Curricular Base (BNCC), in 2018, and the training of teachers who teach Mathematics. We seek to highlight possible weaknesses in initial and continuing education in the field of Stochastics, in the face of new educational demands. It is a qualitative research, of a bibliographic and documentary nature, which analyzes official documents that guide basic education and recently published research, which characterize the difficulties faced and point to alternatives for the promotion of literacy, reasoning and stochastic thinking. At the end, we present some proposals that can contribute to achieving the central objectives of Stochastic education, in line with the BNCC.Este artículo tiene como objetivo identificar algunos de los principales desafíos para la enseñanza de la Estocástica — Estadística, Probabilidad y Combinatoria — en la educación básica brasileña, que atiende a alumnos de seis a diecisiete años de edad, a partir de la reforma curricular, desencadenada por la publicación del Base Nacional Común Curricular (BNCC), en 2018, y la formación de docentes que imparten Matemática. Buscamos evidenciar posibles debilidades en la formación inicial y continua en el campo de la Estocástica, frente a las nuevas demandas educativas. Se trata de una investigación cualitativa, de carácter bibliográfico y documental, que analiza documentos oficiales que orientan la educación básica e investigaciones de reciente publicación, que caracterizan las dificultades enfrentadas y apuntan alternativas para la promoción de la lectoescritura, el razonamiento y el pensamiento estocástico. Al final, presentamos algunas propuestas que pueden contribuir al logro de los objetivos centrales de la educación estocástica, en línea con la BNCC.Esse artigo tem por objetivo identificar alguns dos principais desafios para o ensino de Estocástica — Estatística, Probabilidade e Combinatória — na educação básica brasileira, que atende estudantes com idades na faixa de seis a dezessete anos, a partir da reforma curricular, desencadeada pela publicação da Base Nacional Comum Curricular (BNCC), em 2018, e a formação de professores que ensinam Matemática. Buscamos evidenciar possíveis fragilidades na formação inicial e continuada no campo da Estocástica, frente às novas demandas educacionais. É uma pesquisa de natureza qualitativa, de cunho bibliográfico e documental, que analisa documentos oficiais norteadores da educação básica e pesquisas recentemente publicadas, que caracterizam as dificuldades enfrentadas e apontam para alternativas para a promoção do letramento, raciocínio e pensamento estocásticos. Ao final, apresentamos algumas propostas que podem contribuir para alcançar os objetivos centrais da educação Estocástica, em consonância com a BNCC

Ensino de Estatística, Probabilidade e Combinatória na Educação Básica:

Esse artigo tem por objetivo identificar alguns dos principais desafios para o ensino de Estocástica — Estatística, Probabilidade e Combinatória — na educação básica brasileira, que atende estudantes com idades na faixa de seis a dezessete anos, a partir da reforma curricular, desencadeada pela publicação da Base Nacional Comum Curricular (BNCC), em 2018, e a formação de professores que ensinam Matemática. Buscamos evidenciar possíveis fragilidades na formação inicial e continuada no campo da Estocástica, frente às novas demandas educacionais. É uma pesquisa de natureza qualitativa, de cunho bibliográfico e documental, que analisa documentos oficiais norteadores da educação básica e pesquisas recentemente publicadas, que caracterizam as dificuldades enfrentadas e apontam para alternativas para a promoção do letramento, raciocínio e pensamento estocásticos. Ao final, apresentamos algumas propostas que podem contribuir para alcançar os objetivos centrais da educação Estocástica, em consonância com a BNCC

Ensino de Estatística, Probabilidade e Combinatória na Educação Básica: : os novos desafios da BNCC

This article aims to identify some of the main challenges for the teaching of Stochastics — Statistics, Probability and Combinatorics — in Brazilian Basic Education (elementary and high school), which serves students aged from six to seventeen years, from the curricular reform, triggered by the publication of the National Common Curricular Base (BNCC), in 2018, and the training of teachers who teach Mathematics. We seek to highlight possible weaknesses in initial and continuing education in the field of Stochastics, in the face of new educational demands. It is a qualitative research, of a bibliographic and documentary nature, which analyzes official documents that guide basic education and recently published research, which characterize the difficulties faced and point to alternatives for the promotion of literacy, reasoning and stochastic thinking. At the end, we present some proposals that can contribute to achieving the central objectives of Stochastic education, in line with the BNCC.Este artículo tiene como objetivo identificar algunos de los principales desafíos para la enseñanza de la Estocástica — Estadística, Probabilidad y Combinatoria — en la educación básica brasileña, que atiende a alumnos de seis a diecisiete años de edad, a partir de la reforma curricular, desencadenada por la publicación del Base Nacional Común Curricular (BNCC), en 2018, y la formación de docentes que imparten Matemática. Buscamos evidenciar posibles debilidades en la formación inicial y continua en el campo de la Estocástica, frente a las nuevas demandas educativas. Se trata de una investigación cualitativa, de carácter bibliográfico y documental, que analiza documentos oficiales que orientan la educación básica e investigaciones de reciente publicación, que caracterizan las dificultades enfrentadas y apuntan alternativas para la promoción de la lectoescritura, el razonamiento y el pensamiento estocástico. Al final, presentamos algunas propuestas que pueden contribuir al logro de los objetivos centrales de la educación estocástica, en línea con la BNCC.Esse artigo tem por objetivo identificar alguns dos principais desafios para o ensino de Estocástica — Estatística, Probabilidade e Combinatória — na educação básica brasileira, que atende estudantes com idades na faixa de seis a dezessete anos, a partir da reforma curricular, desencadeada pela publicação da Base Nacional Comum Curricular (BNCC), em 2018, e a formação de professores que ensinam Matemática. Buscamos evidenciar possíveis fragilidades na formação inicial e continuada no campo da Estocástica, frente às novas demandas educacionais. É uma pesquisa de natureza qualitativa, de cunho bibliográfico e documental, que analisa documentos oficiais norteadores da educação básica e pesquisas recentemente publicadas, que caracterizam as dificuldades enfrentadas e apontam para alternativas para a promoção do letramento, raciocínio e pensamento estocásticos. Ao final, apresentamos algumas propostas que podem contribuir para alcançar os objetivos centrais da educação Estocástica, em consonância com a BNCC

Polyphenolic Composition and Antioxidant Activity (ORAC, EPR and Cellular) of Different Extracts of Argylia radiata Vitroplants and Natural Roots

Plant biochemistry studies have increased in recent years due to their potential to improve human health. Argylia radiata is an extremophile plant with an interesting polyphenolic profile. However, its biomass is scarce and occasionally available. Argylia in vitro biomass was obtained from tissue culture and compared with in vivo roots regarding its polyphenolic and flavonoid content. Different solvents were used to prepare extracts from the in vitro tissue of callus and aerial plant organs and in vivo roots. UPLC-MS/MS was used to assess the chemical composition of each extract. ORAC-FL and scavenging of free radicals (DPPH and OH) methods were used to determine the antioxidant capacity of extracts. Furthermore, the biological activity of the extracts was established using the cellular antioxidant activity method. The vitroplants were a good source of polyphenols (25–68 mg GAE/100 g tissue FW), and methanol was the most efficient solvent. Eight polyphenolic compounds were identified, and their antioxidant properties were investigated by different chemical methods with EPR demonstrating its specific scavenging activity against free radicals. All extracts showed cellular dose-dependent antioxidant activity. The methanolic extract of vitroplants showed the highest cellular antioxidant activity (44.6% and 51%) at 1 and 10 µg/mL of extract, respectively. Vitroplants of A. radiata are proposed as a biotechnological product as a source of antioxidant compounds with multiple applications

Pan-Cancer Analysis of lncRNA Regulation Supports Their Targeting of Cancer Genes in Each Tumor Context

Long noncoding RNAs (lncRNAs) are commonly dys-regulated in tumors, but only a handful are known toplay pathophysiological roles in cancer. We inferredlncRNAs that dysregulate cancer pathways, onco-genes, and tumor suppressors (cancer genes) bymodeling their effects on the activity of transcriptionfactors, RNA-binding proteins, and microRNAs in5,185 TCGA tumors and 1,019 ENCODE assays.Our predictions included hundreds of candidateonco- and tumor-suppressor lncRNAs (cancerlncRNAs) whose somatic alterations account for thedysregulation of dozens of cancer genes and path-ways in each of 14 tumor contexts. To demonstrateproof of concept, we showed that perturbations tar-geting OIP5-AS1 (an inferred tumor suppressor) andTUG1 and WT1-AS (inferred onco-lncRNAs) dysre-gulated cancer genes and altered proliferation ofbreast and gynecologic cancer cells. Our analysis in-dicates that, although most lncRNAs are dysregu-lated in a tumor-specific manner, some, includingOIP5-AS1, TUG1, NEAT1, MEG3, and TSIX, synergis-tically dysregulate cancer pathways in multiple tumorcontexts

Pan-cancer Alterations of the MYC Oncogene and Its Proximal Network across the Cancer Genome Atlas

Although theMYConcogene has been implicated incancer, a systematic assessment of alterations ofMYC, related transcription factors, and co-regulatoryproteins, forming the proximal MYC network (PMN),across human cancers is lacking. Using computa-tional approaches, we define genomic and proteo-mic features associated with MYC and the PMNacross the 33 cancers of The Cancer Genome Atlas.Pan-cancer, 28% of all samples had at least one ofthe MYC paralogs amplified. In contrast, the MYCantagonists MGA and MNT were the most frequentlymutated or deleted members, proposing a roleas tumor suppressors.MYCalterations were mutu-ally exclusive withPIK3CA,PTEN,APC,orBRAFalterations, suggesting that MYC is a distinct onco-genic driver. Expression analysis revealed MYC-associated pathways in tumor subtypes, such asimmune response and growth factor signaling; chro-matin, translation, and DNA replication/repair wereconserved pan-cancer. This analysis reveals insightsinto MYC biology and is a reference for biomarkersand therapeutics for cancers with alterations ofMYC or the PMN

Genomic, Pathway Network, and Immunologic Features Distinguishing Squamous Carcinomas

This integrated, multiplatform PanCancer Atlas study co-mapped and identified distinguishing molecular features of squamous cell carcinomas (SCCs) from five sites associated with smokin

Spatial Organization and Molecular Correlation of Tumor-Infiltrating Lymphocytes Using Deep Learning on Pathology Images

Beyond sample curation and basic pathologic characterization, the digitized H&E-stained images of TCGA samples remain underutilized. To highlight this resource, we present mappings of tumorinfiltrating lymphocytes (TILs) based on H&E images from 13 TCGA tumor types. These TIL maps are derived through computational staining using a convolutional neural network trained to classify patches of images. Affinity propagation revealed local spatial structure in TIL patterns and correlation with overall survival. TIL map structural patterns were grouped using standard histopathological parameters. These patterns are enriched in particular T cell subpopulations derived from molecular measures. TIL densities and spatial structure were differentially enriched among tumor types, immune subtypes, and tumor molecular subtypes, implying that spatial infiltrate state could reflect particular tumor cell aberration states. Obtaining spatial lymphocytic patterns linked to the rich genomic characterization of TCGA samples demonstrates one use for the TCGA image archives with insights into the tumor-immune microenvironment