19 research outputs found
Transcriptional and Proteomic Analysis of a Ferric Uptake Regulator (Fur) Mutant of Shewanella oneidensis: Possible Involvement of Fur in Energy Metabolism, Transcriptional Regulation, and Oxidative Stress
The iron-directed, coordinate regulation of genes depends on the fur (ferric uptake regulator) gene product, which acts as an iron-responsive, transcriptional repressor protein. To investigate the biological function of a fur homolog in the dissimilatory metal-reducing bacterium Shewanella oneidensis MR-1, a fur knockout strain (FUR1) was generated by suicide plasmid integration into this gene and characterized using phenotype assays, DNA microarrays containing 691 arrayed genes, and two-dimensional polyacrylamide gel electrophoresis. Physiological studies indicated that FUR1 was similar to the wild-type strain when they were compared for anaerobic growth and reduction of various electron acceptors. Transcription profiling, however, revealed that genes with predicted functions in electron transport, energy metabolism, transcriptional regulation, and oxidative stress protection were either repressed (ccoNQ, etrA, cytochrome b and c maturation-encoding genes, qor, yiaY, sodB, rpoH, phoB, and chvI) or induced (yggW, pdhC, prpC, aceE, fdhD, and ppc) in the fur mutant. Disruption of fur also resulted in derepression of genes (hxuC, alcC, fhuA, hemR, irgA, and ompW) putatively involved in iron uptake. This agreed with the finding that the fur mutant produced threefold-higher levels of siderophore than the wild-type strain under conditions of sufficient iron. Analysis of a subset of the FUR1 proteome (i.e., primarily soluble cytoplasmic and periplasmic proteins) indicated that 11 major protein species reproducibly showed significant (P < 0.05) differences in abundance relative to the wild type. Protein identification using mass spectrometry indicated that the expression of two of these proteins (SodB and AlcC) correlated with the microarray data. These results suggest a possible regulatory role of S. oneidensis MR-1 Fur in energy metabolism that extends the traditional model of Fur as a negative regulator of iron acquisition systems
Identification of Membrane Proteins in the Hyperthermophilic Archaeon Pyrococcus Furiosus Using Proteomics and Prediction Programs
Cell-free extracts from the hyperthermophilic archaeon Pyrococcus furiosus were
separated into membrane and cytoplasmic fractions and each was analyzed by 2D-gel
electrophoresis. A total of 66 proteins were identified, 32 in the membrane fraction and 34
in the cytoplasmic fraction. Six prediction programs were used to predict the subcellular
locations of these proteins. Three were based on signal-peptides (SignalP, TargetP, and
SOSUISignal) and three on transmembrane-spanning α-helices (TSEG, SOSUI, and
PRED-TMR2). A consensus of the six programs predicted that 23 of the 32 proteins
(72%) from the membrane fraction should be in the membrane and that all of the proteins
from the cytoplasmic fraction should be in the cytoplasm. Two membrane-associated
proteins predicted to be cytoplasmic by the programs are also predicted to consist
primarily of transmembrane-spanning β-sheets using porin protein models, suggesting that
they are, in fact, membrane components. An ATPase subunit homolog found in the
membrane fraction, although predicted to be cytoplasmic, is most likely complexed with
other ATPase subunits in the membrane fraction. An additional three proteins predicted to
be cytoplasmic but found in the membrane fraction, may be cytoplasmic contaminants.
These include a chaperone homolog that may have attached to denatured membrane
proteins during cell fractionation. Omitting these three proteins would boost the
membrane-protein predictability of the models to near 80%. A consensus prediction using
all six programs for all 2242 ORFs in the P. furiosus genome estimates that 24% of the
ORF products are found in the membrane. However, this is likely to be a minimum value
due to the programs’ inability to recognize certain membrane-related proteins, such as
subunits associated with membrane complexes and porin-type proteins
Knock-out of SO1377 gene, which encodes the member of a conserved hypothetical bacterial protein family COG2268, results in alteration of iron metabolism, increased spontaneous mutation and hydrogen peroxide sensitivity in Shewanella oneidensis MR-1
BACKGROUND: Shewanella oneidensis MR-1 is a facultative, gram-negative bacterium capable of coupling the oxidation of organic carbon to a wide range of electron acceptors such as oxygen, nitrate and metals, and has potential for bioremediation of heavy metal contaminated sites. The complete 5-Mb genome of S. oneidensis MR-1 was sequenced and standard sequence-comparison methods revealed approximately 42% of the MR-1 genome encodes proteins of unknown function. Defining the functions of hypothetical proteins is a great challenge and may need a systems approach. In this study, by using integrated approaches including whole genomic microarray and proteomics, we examined knockout effects of the gene encoding SO1377 (gi24372955), a member of the conserved, hypothetical, bacterial protein family COG2268 (Clusters of Orthologous Group) in bacterium Shewanella oneidensis MR-1, under various physiological conditions. RESULTS: Compared with the wild-type strain, growth assays showed that the deletion mutant had a decreased growth rate when cultured aerobically, but not affected under anaerobic conditions. Whole-genome expression (RNA and protein) profiles revealed numerous gene and protein expression changes relative to the wild-type control, including some involved in iron metabolism, oxidative damage protection and respiratory electron transfer, e. g. complex IV of the respiration chain. Although total intracellular iron levels remained unchanged, whole-cell electron paramagnetic resonance (EPR) demonstrated that the level of free iron in mutant cells was 3 times less than that of the wild-type strain. Siderophore excretion in the mutant also decreased in iron-depleted medium. The mutant was more sensitive to hydrogen peroxide and gave rise to 100 times more colonies resistant to gentamicin or kanamycin. CONCLUSION: Our results showed that the knock-out of SO1377 gene had pleiotropic effects and suggested that SO1377 may play a role in iron homeostasis and oxidative damage protection in S. oneidensis MR-1
Comparative systems biology across an evolutionary gradient within the Shewanella genus
Author Posting. © The Authors, 2009. This is the author's version of the work. It is posted here by permission of National Academy of Sciences for personal use, not for redistribution. The definitive version was published in Proceedings of the National Academy of Sciences 106 (2009): 15909-15914, doi:10.1073/pnas.0902000106.To what extent genotypic differences translate to phenotypic variation remains a poorly
understood issue of paramount importance for several cornerstone concepts of microbiology
including the species definition. Here, we take advantage of the completed genomic sequences,
expressed proteomic profiles, and physiological studies of ten closely related Shewanella
strains and species to provide quantitative insights into this issue. Our analyses revealed that,
despite extensive horizontal gene transfer within these genomes, the genotypic and phenotypic
similarities among the organisms were generally predictable from their evolutionary
relatedness. The power of the predictions depended on the degree of ecological specialization
of the organisms evaluated. Using the gradient of evolutionary relatedness formed by these
genomes, we were able to partly isolate the effect of ecology from that of evolutionary
divergence and rank the different cellular functions in terms of their rates of evolution. Our
ranking also revealed that whole-cell protein expression differences among these organisms
when grown under identical conditions were relatively larger than differences at the genome
level, suggesting that similarity in gene regulation and expression should constitute another
important parameter for (new) species description. Collectively, our results provide important
new information towards beginning a systems-level understanding of bacterial species and
genera.The authors have
been supported by the DOE through the Shewanella Federation consortium and the Proteomics
Application project. The MSU work relevant to speciation was also supported by NSF (DEB
0516252)
The influence of cultivation methods on Shewanella oneidensis physiology and proteome expression
High-throughput analyses that are central to microbial systems biology and ecophysiology research benefit from highly homogeneous and physiologically well-defined cell cultures. While attention has focused on the technical variation associated with high-throughput technologies, biological variation introduced as a function of cell cultivation methods has been largely overlooked. This study evaluated the impact of cultivation methods, controlled batch or continuous culture in bioreactors versus shake flasks, on the reproducibility of global proteome measurements in Shewanellaoneidensis MR-1. Variability in dissolved oxygen concentration and consumption rate, metabolite profiles, and proteome was greater in shake flask than controlled batch or chemostat cultures. Proteins indicative of suboxic and anaerobic growth (e.g., fumarate reductase and decaheme c-type cytochromes) were more abundant in cells from shake flasks compared to bioreactor cultures, a finding consistent with data demonstrating that “aerobic” flask cultures were O2 deficient due to poor mass transfer kinetics. The work described herein establishes the necessity of controlled cultivation for ensuring highly reproducible and homogenous microbial cultures. By decreasing cell to cell variability, higher quality samples will allow for the interpretive accuracy necessary for drawing conclusions relevant to microbial systems biology research
GELBANK: a database of annotated two-dimensional gel electrophoresis patterns of biological systems with completed genomes
GELBANK is a publicly available database of two-dimensional gel electrophoresis (2DE) gel patterns of proteomes from organisms with known genome information (available at http://gelbank.anl.gov and ftp://bioinformatics.anl.gov/gelbank/). Currently it includes 131 completed, mostly microbial proteomes available from the National Center for Biotechnology Information. A web interface allows the upload of 2D gel patterns and their annotation for registered users. The images are organized by species, tissue type, separation method, sample type and staining method. The database can be queried based on protein or 2DE-pattern attributes. A web interface allows registered users to assign molecular weight and pH gradient profiles to their own 2D gel patterns as well as to link protein identifications to a given spot on the pattern. The website presents all of the submitted 2D gel patterns where the end-user can dynamically display the images or parts of images along with molecular weight, pH profile information and linked protein identification. A collection of images can be selected for the creation of animations from which the user can select sub-regions of interest and unlimited 2D gel patterns for visualization. The website currently presents 233 identifications for 81 gel patterns for Homo sapiens, Methanococcus jannaschii, Pyro coccus furiosus, Shewanella oneidensis, Escherichia coli and Deinococcus radiodurans
Differential recovery of biotinylated microbial proteins using monomeric or polymeric avidin
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Differential Protein Expression in the Metal-Reducing Bacterium Geobacter Sulfurreducens Strain PCA Grown with Fumarate or Ferric Citrate
Geobacter sulfurreducens, generally considered to be a strict anaerobe, is a predominant microbe in subsurface environments, where it utilizes available metals as electron acceptors. To better understand the metabolic processes involved in the metal-reduction capability of this microbe, the proteins expressed by cells grown anaerobically with either fumarate or ferric citrate as electron acceptor were compared. Proteins were separated by 2-DE under denaturing or nondenaturing conditions, and proteins varying in abundance with a high level of statistical significance (p\u3c0.0001) were identified by peptide mass analysis. Denaturing 2-DE revealed significant differences in the relative abundance of the membrane proteins OmpA and peptidoglycan-associated lipoprotein, several metabolic enzymes, and, in addition, superoxide dismutase and rubredoxin oxidoreductase. Nondenaturing 2-DE revealed elevated catalase in cells grown with ferric citrate. These results suggest that, in addition to adjustments in membrane transport and specific metabolic pathways in response to these two different electron acceptors, distinct differences exist in the oxidative environment within the cell when fumarate or soluble ferric citrate is provided as electron acceptor. Although an anaerobe, G. sulfurreducens appears to have alternate mechanisms for dealing with reactive oxygen species in response to increased intracellular soluble iron
An End-to-End System for Organizing and Sharing Raw and Derived Mass Spectrometry Data
With the increasing amount of work being performed in the field of Mass Spectrometry (MS), a huge amount of data is being generated. This data needs to be properly managed, organized and shared among researchers at various institutions. The problem is further complicated by the different proprietary formats used by manufacturers of MS machines. We demonstrate an end-toend system to automate the process of converting the data to an open format, and to upload the data to a centralized server where it is easily organized and managed. The system allows scientists to browse, download, and use the data with third party tools. The user-view is simple and hides the underlying data-management system.