Chronology of Range Expansion of the Coyote, Canis latrans, in New York

Coyotes (Canis latrans) were historically restricted to central North America. In less than two centuries, however, Coyotes have colonized most of the continent, including much of northeastern North America. Better understanding causes and proximate mechanisms of this expansion requires a detailed understanding of how Coyotes colonized areas on a fine scale. We examined the establishment of Coyotes in the State of New York by collecting and analyzing reports of their first occurrence throughout the state over the past century, and creating a detailed map of range expansion. Coyotes first entered New York from the north, circled the Adirondack region prior to colonizing it, and then expanded southward and westward at ca. 78-90 km/decade. The revealed pattern lends little support to the hypotheses that the range expansion is attributable to translocations and releases, or that Coyotes were historically present in the region and only recently expanded in numbers. Rather, the data suggest a correlative relationship between anthropogenic land use and Coyote range expansion

Influence of Type of Cigarette on Peripheral versus Central Lung Cancer

Objectives: Adenocarcinoma has replaced squamous cell carcinoma as the most common cell type of lung cancer in the United States. It has been proposed that this shift is due to the increased use of filter and lower-tar cigarettes, resulting in increased delivery of smoke to peripheral regions of the lungs, where adenocarcinoma usually occurs. We reviewed radiologic data to evaluate the hypothesis that tumors in smokers of cigarettes with lower-tar yield are more likely to occur peripherally than tumors in smokers of higher-yield cigarettes. Methods: At two urban academic medical centers, we reviewed computed tomographic scans, chest radiographs, and medical records to assign tumor location (peripheral or central) for 330 smokers diagnosed with carcinoma of the lung between 1993 and 1999. We compared the proportion of tumors in a peripheral versus central location by lifetime filter use and average lifetime tar rating (<21 and ≥21 mg). Results: Tumor location (69% peripheral and 31% central) was unrelated to cigarette filter use. Smokers of cigarettes with lower-tar ratings were more likely than those with higher ratings to have peripheral rather than central tumors (odds ratio, 1.76; 95% confidence interval, 0.89-3.47). When restricted to subjects with adenocarcinoma or squamous cell carcinoma, the odds ratio (95% confidence interval) was 2.31 (1.05-5.08). Conclusions: Among cigarette smokers with lung cancer, use of cigarettes with lower-tar yield was associated with preferential occurrence of tumors in peripheral sites. Our findings support the hypothesis that changes in smoking associated with lower-tar cigarettes have led to a shift in the location of smoking-related lung cancer

Multiple Paternity in a Reintroduced Population of the Orinoco Crocodile (Crocodylus intermedius) at the El Frío Biological Station, Venezuela

The success of a reintroduction program is determined by the ability of individuals to reproduce and thrive. Hence, an understanding of the mating system and breeding strategies of reintroduced species can be critical to the success, evaluation and effective management of reintroduction programs. As one of the most threatened crocodile species in the world, the Orinoco crocodile (Crocodylus intermedius) has been reduced to only a few wild populations in the Llanos of Venezuela and Colombia. One of these populations was founded by reintroduction at Caño Macanillal and La Ramera lagoon within the El Frío Biological Station, Venezuela. Twenty egg clutches of C. intermedius were collected at the El Frío Biological Station for incubation in the lab and release of juveniles after one year. Analyzing 17 polymorphic microsatellite loci from 335 hatchlings we found multiple paternity in C. intermedius, with half of the 20 clutches fathered by two or three males. Sixteen mothers and 14 fathers were inferred by reconstruction of multilocus parental genotypes. Our findings showed skewed paternal contributions to multiple-sired clutches in four of the clutches (40%), leading to an overall unequal contribution of offspring among fathers with six of the 14 inferred males fathering 90% of the total offspring, and three of those six males fathering more than 70% of the total offspring. Our results provide the first evidence of multiple paternity occurring in the Orinoco crocodile and confirm the success of reintroduction efforts of this critically endangered species in the El Frío Biological Station, Venezuela

High levels of population genetic differentiation in the American crocodile (Crocodylus acutus)

The American crocodile (Crocodylus acutus) is a widely distributed species across coastal and brackish areas of the Neotropical region of the Americas and the Greater Antilles. Available information on patterns of genetic differentiation in C. acutus shows a complex structuring influenced by interspecific interactions (mainly hybridization) and anthropogenic actions (mostly historical hunting, recent poaching, habitat loss and fragmentation, and unintentional translocation of individuals). In this study, we used data on mitochondrial DNA control region and 11 nuclear polymorphic microsatellite loci to assess the degree of population structure of C. acutus in South America, North America, Central America and the Greater Antilles. We used traditional genetic differentiation indices, Bayesian clustering and multivariate methods to create a more comprehensive picture of the genetic relationships within the species across its range. Analyses of mtDNA and microsatellite loci show evidence of a strong population genetic structure in the American crocodile, with unique populations in each sampling locality. Our results support previous findings showing large degrees of genetic differentiation between the continental and the Greater Antillean C. acutus. We report three new haplotypes unique to Venezuela, which are considerably less distant from the Central and North American haplotypes than to the Greater Antillean ones. Our findings reveal genetic population differentiation between Cuban and Jamaican C. acutus and offer the first evidence of strong genetic differentiation among the populations of Greater Antillean C. acutus

Risky business: Modeling mortality risk near the urban-wildland interface for a large carnivore

We examined the spatial distribution of 382 black bear (Ursus americanus) mortalities in the Lake Tahoe Basin and Western Great Basin Desert (WGB) in Nevada, USA from 1997 to 2013. Of the 364 mortalities for which we could determine cause of death, vehicle collisions (n = 160) and direct removal of bears by management personnel (n = 132) were the two largest sources of mortality for bears in our study area at the confluence of the Sierra-Nevada Mountains and the Great Basin Desert. Here we use logistic regression and resource selection probability functions (RSPF) to model probability of mortality in the WGB based on anthropogenic and landscape variables. Further, we assessed the impact of spatial resolution on our analyses and resulting probability of mortality models. Human-induced mortalities of black bears were overwhelmingly concentrated near major roads (defined in our analyses as paved roads with two lanes or more), in the town of Incline Village, Nevada, and along the foothills of the Carson Range east of Lake Tahoe. Our results suggest mortality risk is associated with density of and distance to multiple forest types, human population density, landcover, recreation site density and distance, road density and distance, stream distance, hiking trail density, and waterbody distance. Our model results found environmental variables measured at coarse spatial resolutions such as distance to and density of forest best predicted black bear mortality risk, while anthropogenic variables measured at fine spatial resolutions like distance to and density of recreation site best predicted black bear mortality risk in our study area. Our results demonstrate that carnivore mortality as a phenomenon likely operates at multiple spatial resolutions and thus considering scale is important for modeling mortality risk on the landscape. Keywords: Carnivore, Black bear, Mortality, RSPF, Scale, Human-wildlife conflic