591 research outputs found

    The 12 prophets dataset

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    The "Ajeijadinho 3D" project is an initiative supported by the University of S\~ao Paulo (Museum of Science and Dean of Culture and Extension), which involves the 3D digitization of art works of Brazilian sculptor Antonio Francisco Lisboa, better known as Aleijadinho. The project made use of advanced acquisition and processing of 3D meshes for preservation and dissemination of the cultural heritage. The dissemination occurs through a Web portal, so that the population has the opportunity to meet the art works in detail using 3D visualization and interaction. The portal address is http://www.aleijadinho3d.icmc.usp.br. The 3D acquisitions were conducted over a week at the end of July 2013 in the cities of Ouro Preto, MG, Brazil and Congonhas do Campo, MG, Brazil. The scanning was done with a special equipment supplied by company Leica Geosystems, which allowed the work to take place at distances between 10 and 30 meters, defining a non-invasive procedure, simplified logistics, and without the need for preparation or isolation of the sites. In Ouro Preto, we digitized the churches of Francisco of Assis, Our Lady of Carmo, and Our Lady of Mercy; in Congonhas do Campo we scanned the entire Sanctuary of Bom Jesus de Matosinhos and his 12 prophets. Once scanned, the art works went through a long process of preparation, which required careful handling of meshes done by experts from the University of S\~ao Paulo in partnership with company Imprimate.Comment: Full dataset online at http://aleijadinho3d.icmc.usp.br/data.htm

    Systematic evaluation of software product line architectures

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    The architecture of a software product line is one of its most important artifacts as it represents an abstraction of the products that can be generated. It is crucial to evaluate the quality attributes of a product line architecture in order to: increase the productivity of the product line process and the quality of the products; provide a means to understand the potential behavior of the products and, consequently, decrease their time to market; and, improve the handling of the product line variability. The evaluation of product line architecture can serve as a basis to analyze the managerial and economical values of a product line for software managers and architects. Most of the current research on the evaluation of product line architecture does not take into account metrics directly obtained from UML models and their variabilities; the metrics used instead are difficult to be applied in general and to be used for quantitative analysis. This paper presents a Systematic Evaluation Method for UML-based Software Product Line Architecture, the SystEM-PLA. SystEM-PLA differs from current research as it provides stakeholders with a means to: (i) estimate and analyze potential products; (ii) use predefined basic UML-based metrics to compose quality attribute metrics; (iii) perform feasibility and trade-off analysis of a product line architecture with respect to its quality attributes; and, (iv) make the evaluation of product line architecture more flexible. An example using the SEI’s Arcade Game Maker (AGM) product line is presented as a proof of concept, illustrating SystEM-PLA activities. Metrics for complexity and extensibility quality attributes are defined and used to perform a trade-off analysis

    What is the best strategy for retaining gestures in working memory?

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    This study aimed to determine whether the recall of gestures in working memory could be enhanced by verbal or gestural strategies. We also attempted to examine whether these strategies could help resist verbal or gestural interference. Fifty-four participants were divided into three groups according to the content of the training session. This included a control group, a verbal strategy group (where gestures were associated with labels) and a gestural strategy group (where participants repeated gestures and were told to imagine reproducing the movements). During the experiment, the participants had to reproduce a series of gestures under three conditions: "no interference", gestural interference (gestural suppression) and verbal interference (articulatory suppression). The results showed that task performance was enhanced in the verbal strategy group, but there was no significant difference between the gestural strategy and control groups. Moreover, compared to the "no interference" condition, performance decreased in the presence of gestural interference, except within the verbal strategy group. Finally, verbal interference hindered performance in all groups. The discussion focuses on the use of labels to recall gestures and differentiates the induced strategies from self-initiated strategies

    Characterization and transferability of microsatellite markers of the cultivated peanut (Arachis hypogaea)

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    BACKGROUND: The genus Arachis includes Arachis hypogaea (cultivated peanut) and wild species that are used in peanut breeding or as forage. Molecular markers have been employed in several studies of this genus, but microsatellite markers have only been used in few investigations. Microsatellites are very informative and are useful to assess genetic variability, analyze mating systems and in genetic mapping. The objectives of this study were to develop A. hypogaea microsatellite loci and to evaluate the transferability of these markers to other Arachis species. RESULTS: Thirteen loci were isolated and characterized using 16 accessions of A. hypogaea. The level of variation found in A. hypogaea using microsatellites was higher than with other markers. Cross-transferability of the markers was also high. Sequencing of the fragments amplified using the primer pair Ah11 from 17 wild Arachis species showed that almost all wild species had similar repeated sequence to the one observed in A. hypogaea. Sequence data suggested that there is no correlation between taxonomic relationship of a wild species to A. hypogaea and the number of repeats found in its microsatellite loci. CONCLUSION: These results show that microsatellite primer pairs from A. hypogaea have multiple uses. A higher level of variation among A. hypogaea accessions can be detected using microsatellite markers in comparison to other markers, such as RFLP, RAPD and AFLP. The microsatellite primers of A. hypogaea showed a very high rate of transferability to other species of the genus. These primer pairs provide important tools to evaluate the genetic variability and to assess the mating system in Arachis species

    Taxa de acúmulo de matéria seca e biomassa aérea de uma pastagem de capim braquiária.

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    Esse estudo faz parte do projeto de pesquisa denominado "Variabilidade espacial dos índices de qualidade do solo para sistemas agropecuários Qualisolo". O objetivo do estudo é a obtenção da taxa de acúmulo de matéria seca e a biomassa aérea de uma pastagem de capim braquiária usualmente encontrada em propriedades rurais brasileiras de criação de gado de corte. No presente trabalho fez-se também, para efeito de comparação, a estimativa de taxa de acúmulo de matéria seca através de modelos empíricos da literatura que utilizam alguns elementos climáticos. O estudo foi realizado na fazenda experimental do Instituto de Zootecnia, em Nova Odessa (SP), de novembro de 2010 a junho de 2011. A pastagem foi manejada sob pastejo contínuo e taxa de lotação variável. A taxa média de acúmulo da forragem no período foi de 50,5 kg MS. ha?¹.dia?¹ e o acúmulo total de matéria seca em 213 dias foi 10.754,4 kg MS. ha?¹. A análise geoestatística da biomassa obtida em 50 pontos de amostragem revelou dependência espacial somente para a biomassa média dos meses agrupados de verão e outono.bitstream/item/58506/1/001-12.pd

    Secondary Metabolites

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    Plants are the main source for obtaining secondary metabolites that are used in the pharmaceutical, cosmetic and food industries. In nature, the performance of bioactive compounds is affected by biotic and abiotic factors, an alternative to overcome this adversity is in vitro plant cultures and particularly plant cell culture that has multiple advantages, highlighting the possibility of controlling variables to increase the content. of compounds of interest. The objective of this research was to determine and optimize the effect of some elicitors on the production of secondary flavonoid metabolites in suspension cell cultures of Thevetia peruviana on a shake flask scale. The experimental part was performed from cell cultures in suspension cells of T. peruviana maintained by the Bioconversion Laboratory of the Universidad Nacional de Colombia, sede Medellin. Firstly, methyl jasmonate (MeJa) 3 µM and salicylic acid (SA) 300 µM were added to two los of cell cultures, that circumstantially they differed in their time suspension state lot 1 (9 months) and lot 2 (3 months). Secondly, certain were evaluated, in order to be optimized; the operational parameters cosidered were: day of addition, concentration and hours of harvest. Finally, the combined effect of MeJa (0.3 µM) and SA (100 µM) was determined in different proportions, the day of elicitation and the hours of harvest were constant. Flavonoid content was quantified by UV-vis spectrophotometry usinfg the AlCl3 complexation method and was evaluated at extracellular and intracellular level. In the first part, at intracellular level, SA generated 14.6 % and 9.56 % more flavonoid content than MeJa in lots 1 and 2, respectively. In the second part, 4.14 mg EQ/g DW were generated with MeJa 0.3 µM, elicitation day 5 and harvest at 90 h; 3.75 mg EQ/g DW were generated with 100 µM SA, elicitation day 0 and harvests at 96 h. In the third part, 4.62 mg EQ / g DW were generated with the combination of MeJa (0.3 µM) – SA (100 µM) in a ratio of 20-80, elicitation on day 0 and harvest at 96 h. Eliciting cell cultures of Thevetia peruviana with MeJa and SA under optimal conditions of concentration, day of addition and hours of harvest increased the content of flavonoid compounds. The results obtained could serve as a basis for the development of investigations at the bioreactor scale.Las plantas son la principal fuente para la obtención de metabolitos secundarios que se usan en la industria farmacéutica, cosmética y alimentaria. En la naturaleza el rendimiento de compuestos bioactivos se ve afectado por factores bióticos y abióticos, una alternativa para superar esta adversidad son los cultivos vegetales in vitro y particularmente el cultivo de células en suspensión que presenta múltiples ventajas destacándose la posibilidad de controlar variables para aumentar el contenido de compuestos de interés. El objetivo del presente trabajo de investigación fue determinar y optimizar el efecto de algunos elicitores sobre la producción de metabolitos secundarios tipo flavonoides en cultivos de células en suspensión de Thevetia peruviana a escala de matraz agitado. La parte experimental se realizó a partir de cultivos de células en células en suspensión de T. peruviana que mantenía el laboratorio de bioconversiones de la Universidad Nacional de Colombia sede Medellín. Primeramente, metil jasmonato (MeJa) 3 μM y ácido salicílico (AS) 300 μM se adicionaron a dos lotes de cultivos de células; que circunstancialmente diferían en su tiempo en estado de suspensión lote 1 (9 meses) y lote 2 (3 meses). Segundamente, determinadas condiciones fueron evaluadas, con el fin de ser optimizadas; los parámetros operacionales que se consideraron fueron: día de adición, concentración y las horas de cosecha. Por último, el efecto combinado de MeJa (0,3 μM) – AS (100 μM) se determinó en diferentes proporciones, el día de elicitación y las horas de cosecha fueron constantes. El contenido de flavonoides se cuantificó por espectrofotometría UV – vis por el método de complejación de AlCl3 y se evaluaron a nivel extracelular e intracelular. En la primera parte, a nivel intracelular AS generaron 14,6 % y 9,56 % más contenido de flavonoides que MeJa en lotes 1 y 2, respectivamente. En la segunda parte, 4,14 mg EQ/g MS fueron generados con MeJa 0,3 μM, elicitación día 5 y cosecha a 90 h; por otro lado, 3,75 mg EQ/g MS fueron generados con AS 100 μM, elicitación día 0 y cosecha a 96 h. En la tercera parte, 4,62 mg EQ/g MS fueron generados con la combinación de MeJa (0,3 μM) – AS (100 μM) en proporción 20 – 80, elicitación el día 0 y cosecha a las 96 h. Elicitar cultivos celulares de Thevetia peruviana con MeJa y AS en condiciones óptimas de concentración, día de adición y horas de cosecha incrementaron el contenido de compuestos flavonoides. Los resultados obtenidos podrían servir como base para el desarrollo de investigaciones a escala de biorreactor.Maestrí
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