Consumption of Methane and CO_2 by Methanotrophic Microbial Mats from Gas Seeps of the Anoxic Black Sea

The deep anoxic shelf of the northwestern Black Sea has numerous gas seeps, which are populated by methanotrophic microbial mats in and above the seafloor. Above the seafloor, the mats can form tall reef-like structures composed of porous carbonate and microbial biomass. Here, we investigated the spatial patterns of CH_4 and CO_2 assimilation in relation to the distribution of ANME groups and their associated bacteria in mat samples obtained from the surface of a large reef structure. A combination of different methods, including radiotracer incubation, beta microimaging, secondary ion mass spectrometry, and catalyzed reporter deposition fluorescence in situ hybridization, was applied to sections of mat obtained from the large reef structure to locate hot spots of methanotrophy and to identify the responsible microbial consortia. In addition, CO_2 reduction to methane was investigated in the presence or absence of methane, sulfate, and hydrogen. The mat had an average δ^(13)C carbon isotopic signature of −67.1‰, indicating that methane was the main carbon source. Regions dominated by ANME-1 had isotope signatures that were significantly heavier (−66.4‰ ± 3.9 ‰ [mean ± standard deviation; n = 7]) than those of the more central regions dominated by ANME-2 (−72.9‰ ± 2.2 ‰; n = 7). Incorporation of ^(14)C from radiolabeled CH_4 or CO_2 revealed one hot spot for methanotrophy and CO2 fixation close to the surface of the mat and a low assimilation efficiency (1 to 2% of methane oxidized). Replicate incubations of the mat with ^(14)CH_4 or ^(14)CO_2 revealed that there was interconversion of CH_4 and CO_2. The level of CO_2 reduction was about 10% of the level of anaerobic oxidation of methane. However, since considerable methane formation was observed only in the presence of methane and sulfate, the process appeared to be a rereaction of anaerobic oxidation of methane rather than net methanogenesis

The Process \gamma^{*}_L+ q \to q\bar{q}g + q: Real Corrections to the Virtual Photon Impact Factor

We calculate, for the longitudinally polarized virtual photon, the cross section of the process \gamma^{*}+q\to (q\bar{q}g)+q at high energies with a large rapidity gap between the fragmentation system q\bar{q}g and the other quark. This process provides the real corrections of the virtual photon impact factor in the next-to leading order. Evidence is given for the appearance of a new q\bar{q}g Fock-component of the photon state.Comment: 17 pages, 4 figures, revte

The (gamma^*-q\bar q)-Reggeon Vertex in Next-to-Leading Order QCD

As a first step towards the computation of the NLO corrections to the photon impact factor in the $\gamma^*\gamma^* \to \gamma^* \gamma^*$ scattering process, we calculate the one loop corrections to the coupling of the reggeized gluon to the $\gamma^*\to q\bar{q}$ vertex. We list the results for the Feynman diagrams which contribute: all loop integrations are carried out, and the results are presented in the helicity basis of photon, quark, and antiquark.Comment: 26 pages LaTeX, 3 figures, typos fixe

NLO corrections to the photon impact factor: Combining real and virtual corrections.

In this third part of our calculation of the QCD NLO corrections to the photon impact factor we combine our previous results for the real corrections with the singular pieces of the virtual corrections and present finite analytic expressions for the quark-antiquark-gluon intermediate state inside the photon impact factor. We begin with a list of the infrared singular pieces of the virtual correction, obtained in the first step of our program. We then list the complete results for the real corrections (longitudinal and transverse photon polarization). In the next step we define, for the real corrections, the collinear and soft singular regions and calculate their contributions to the impact factor. We then subtract the contribution due to the central region. Finally, we combine the real corrections with the singular pieces of the virtual corrections and obtain our finite results

Spray nozzle designs for agricultural aviation applications

Techniques of generating monodisperse sprays and information concerning chemical liquids used in agricultural aviation are surveyed. The periodic dispersion of liquid jet, the spinning disk method, and ultrasonic atomization are the techniques discussed. Conceptually designed spray nozzles for generating monodisperse sprays are assessed. These are based on the classification of the drops using centrifugal force, on using two opposing liquid laden air jets, and on operating a spinning disk at an overloaded flow. Performance requirements for the designs are described and estimates of the operational characteristics are presented

Hookworm infection in the Australian sea lion (Neophoca cinerea)

For the Australian sea lion (Neophoca cinerea), an endangered keystone predator that demonstrates high rates of pup mortality and limited population recovery, an understanding of the role of infectious disease in influencing pup health, and how it may contribute towards shaping population demography, is a key knowledge gap. This thesis investigated the taxonomy, epidemiology, clinical impact, and management of hookworm infection in N. cinerea to address the hypothesis that hookworm infection is a significant cause of disease and mortality in this species. Hookworms collected from N. cinerea pups were identified and described as a novel species (Uncinaria sanguinis). Transmammary transmission in the immediate post-parturient period was implicated as the predominant route leading to patent hookworm infection in pups; however, in contrast to the fundamental role that colony substrate appears to play in shaping the epidemiology of hookworm infection in other otariid hosts, this thesis determined that all N. cinerea pups are infected with U. sanguinis irrespective of the type of colony substrate and that the intensity of hookworm infection appears to be influenced by colony-specific seasonal differences in host behaviour. The clinical impact of hookworm infection in pups was quantified and the occurrence of seasonal patterns in health parameters and the magnitude of colony pup mortality were related to the dynamics of hookworm infection. In addition, the effectiveness of ivermectin to eliminate hookworm infection was investigated. This thesis determined that U. sanguinis is an important cause of disease and mortality in N. cinerea; this thesis contributes towards an improved understanding of the role of infectious disease in influencing the health status and population demography of this endangered species, informing conservation management and providing a solid foundation for further investigations of the effect of disease on the health status of free-ranging species

Implications of non-linearities between cumulative CO2 emissions and CO2-induced warming for assessing the remaining carbon budget

To determine the remaining carbon budget, a new framework was introduced in the Intergovernmental Panel on Climate Change's Special Report on Global Warming of 1.5 °C (SR1.5). We refer to this as a 'segmented' framework because it considers the various components of the carbon budget derivation independently from one another. Whilst implementing this segmented framework, in SR1.5 the assumption was that there is a strictly linear relationship between cumulative CO2 emissions and CO2-induced warming i.e. the TCRE is constant and can be applied to a range of emissions scenarios. Here we test whether such an approach is able to replicate results from model simulations that take the climate system's internal feedbacks and non-linearities into account. Within our modelling framework, following the SR1.5's choices leads to smaller carbon budgets than using simulations with interacting climate components. For 1.5 °C and 2 °C warming targets, the differences are 50 GtCO2 (or 10%) and 260 GtCO2 (or 17%), respectively. However, by relaxing the assumption of strict linearity, we find that this difference can be reduced to around 0 GtCO2 for 1.5 °C of warming and 80 GtCO2 (or 5%) for 2.0 °C of warming (for middle of the range estimates of the carbon cycle and warming response to anthropogenic emissions). We propose an updated implementation of the segmented framework that allows for the consideration of non-linearities between cumulative CO2 emissions and CO2-induced warming

Spacetime colour reconnection in Herwig 7

We present a model for generating spacetime coordinates in the Monte Carlo event generator Herwig 7, and perform colour reconnection by minimizing a boost-invariant distance measure of the system. We compare the model to a series of soft physics observables. We find reasonable agreement with the data, suggesting that pp-collider colour reconnection may be able to be applied in larger systems

The aT distribution of the Z boson at hadron colliders

We provide the first theoretical study of a novel variable, $a_T$, proposed in Ref.[1] as a more accurate probe of the region of low transverse momentum $p_T$, for the $Z$ boson $p_T$ distribution at hadron colliders. The $a_T$ is the component of $p_T$ transverse to a suitably defined axis. Our study involves resummation of large logarithms in $a_T$ up to the next-to--leading logarithmic accuracy and we compare the results to those for the well-known $p_T$ distribution, identifying important physical differences between the two cases. We also test our resummed result at the two-loop level by comparing its expansion to order $\alpha_s^2$ with the corresponding fixed-order results and find agreement with our expectations.Comment: 30 pages, 3 figures, JHEP class included. Final version published in JHE

Packaging and preservation of space-vehicle hardware Final report, 9 Jun. 1966 - 8 Jun. 1967

Packaging and preservation of space vehicle hardwar