33 research outputs found
The effect of halogenation on PBDTT-TQxT based non-fullerene polymer solar cells – Chlorination vs fluorination
Expression-Enhanced Fluorescent Proteins Based on Enhanced Green Fluorescent Protein for Super-resolution Microscopy
“Smart fluorophores”, such as reversibly switchable fluorescent proteins, are crucial for advanced fluorescence imaging. However, only a limited number of such labels is available, and many display reduced biological performance compared to more classical variants. We present the development of robustly photoswitchable variants of enhanced green fluorescent protein (EGFP), named rsGreens, that display up to 30-fold higher fluorescence in E. coli colonies grown at 37 ?C and more than 4-fold higher fluorescence when expressed in HEK293T cells compared to their ancestor protein rsEGFP. This enhancement is not due to an intrinsic increase in the fluorescence brightness of the probes, but rather due to enhanced expression levels that allow many more probe molecules to be functional at any given time. We developed rsGreens displaying a range of photoswitching kinetics and show how these can be used for multimodal diffraction-unlimited fluorescence imaging such as pcSOFI and RESOLFT,
achieving a spatial resolution of ∼70 nm. By determining the first ever crystal structures of a negative reversibly switchable FP derived from Aequorea victoria in both the “on”- and “off”-conformation we were able to confirm the presence of a cis?trans isomerization and provide further insights into the mechanisms underlying the photochromism. Our work demonstrates that genetically encoded “smart fluorophores” can be readily optimized for biological performance and provides a practical strategy for developing maturation- and stability-enhanced photochromic fluorescent proteins.status: publishe
Expression-enhanced fluorescent proteins based on enhanced green fluorescent protein for super-resolution microscopy
International audience“Smart fluorophores”, such as reversibly switchable fluorescent proteins, are crucial for advanced fluorescence imaging. However, only a limited number of such labels is available, and many display reduced biological performance compared to more classical variants. We present the development of robustly photoswitchable variants of enhanced green fluorescent protein (EGFP), named rsGreens, that display up to 30-fold higher fluorescence in E. coli colonies grown at 37 °C and more than 4-fold higher fluorescence when expressed in HEK293T cells compared to their ancestor protein rsEGFP. This enhancement is not due to an intrinsic increase in the fluorescence brightness of the probes, but rather due to enhanced expression levels that allow many more probe molecules to be functional at any given time. We developed rsGreens displaying a range of photoswitching kinetics and show how these can be used for multimodal diffraction-unlimited fluorescence imaging such as pcSOFI and RESOLFT, achieving a spatial resolution of ∼70 nm. By determining the first ever crystal structures of a negative reversibly switchable FP derived from Aequorea victoria in both the “on”- and “off”-conformation we were able to confirm the presence of a cis–trans isomerization and provide further insights into the mechanisms underlying the photochromism. Our work demonstrates that genetically encoded “smart fluorophores” can be readily optimized for biological performance and provides a practical strategy for developing maturation- and stability-enhanced photochromic fluorescent proteins
Tuning Electronic and Morphological Properties for High-Performance Wavelength-Selective Organic Near-Infrared Cavity Photodetectors
Correction to:Tuning Electronic and Morphological Properties for High-Performance Wavelength-Selective Organic Near-Infrared Cavity Photodetectors
Structurally Pure and Reproducible Polymer Materials for High-Performance Organic Solar Cells
The
commercial uptake of (polymer-based) organic solar cells is
among others hindered by poor reproducibility of the device performance,
arising from the variability in molar mass distribution and the presence
of structural defects in push–pull conjugated polymers. Traditional
“in-flask” synthesis methods and commonly used catalysts
contribute to these issues. Flow chemistry has been proposed to provide
consistent molar masses, while a recently applied Buchwald catalyst
shows promise for reduced structural defect formation. However, this
catalyst has not been used for donor polymers affording state-of-the-art
efficiencies in organic solar cells, such as PM6 and D18. In this
work, we utilize these two polymers as model systems to probe the
effect of different synthetic conditions and examine the precise chemical
structures, including any homocoupled defects present, by matrix-assisted
laser desorption/ionization time-of-flight (MALDI–ToF) mass
spectrometry. Additionally, we analyze how these structural factors
impact the material and device properties. By combining droplet-flow
chemistry and defect-free synthesis, we demonstrate a reproducible
and scalable protocol for the synthesis of donor polymers with a tailorable
molar mass for high-efficiency organic photovoltaics
Thin-film photodetectors for NIR and SWIR Image Sensors with 0.13 μm tech node CMOS read-out
status: Published onlin
Organic- and QD-based image sensors integrated on 0.13 μm CMOS ROIC for high resolution, multispectral infrared imaging
status: publishe