Primary vaginal leiomyosarcoma, a rare tumour: case report and review

Primary vaginal leiomyosarcomas (pvLMS) are rare, recurrent tumours accounting for ca. 2% of all vaginal cancers. The etiology is still unknown, the prognosis is poor and there is no consensus guideline on its management. Diagnosis is usually made during the 5th decade due to the presence of a vaginal mass or nodule [1-2]. Current medical literature reports about 200 cases (PubMed®); only 3 studies have considered the ultrastructure [2-4]. Herein a pvLMS is presented and discussed. A nodular, 25 x 23 x 28 mm-mass, infiltrating the urethra but not the rectovaginal septum, was widely excised from the superior vaginal wall of a 58-year-old previously hysterectomized woman. Macroscopic images and MRI were performed. Iliac lymph nodes and HMB45 were negative. The sample was fixed and prepared for light microscopy, transmission (TEM) and scanning (SEM) electron microscopy. Semithin sections showed a storiform pattern of spindle shaped cells with blunt-ended nuclei. Cells arranged in interwoven fascicles within a dense and richly vascularised stroma (neoangiogenesis). Some atypic mitotic figures and focal necrosis were seen. SEM evidenced a dense collagenous stroma with numerous microvessels. TEM showed neoplastic and pleomorphic cells with complex cytoplasm projections containing paranuclear crowds of dilated mitochondria, free ribosomes and a well-developed rough endoplasmic reticulum. Nuclei were large, mostly hyperchromatic, usually indented, with prominent nucleoli and nucleolonema. The dense intercellular space contained dense bundles of collagen fibers. A high and reactive endothelium lined blood vessels. After 4 follow-ups, the patient is fine and without recurrence. Best outcomes occur when the tumour is small, localized, and can be removed surgically with wide, clear margins, as it was for this case. As there are different kinds of LMS, biopsy followed by immunohistochemistry and electron microscopy still represents a good diagnostic choice. References [1] Umeadi et al. (2008) Vaginal leiomyosarcoma. J Obstet Gynaecol 28(5): 553-554. [2] Tobon et al. (1973) Primary leiomyosarcoma of the vagina. Light and electron microscopic observations. Cancer 32(2): 450-457. [3] Akhtar et al. (1978) Primary leiomyosarcoma of the vagina: light and electron microscopic study of a case with review of literature. Tex Med 74(9): 67-71. [4] Rastogi et al. (1984) Primary leiomyosarcoma of the vagina: a study of five cases. Gynecol Oncol 18(1): 77-86

The Mathematical description of lactation curves in dairy cattle

This review gives an overview of the mathematical modelling of lactation curves in dairy cattle. Over the last ninety years, the development of this field of study has followed the main requirements of the dairy cattle industry. Non-linear parametric functions have represented the preferred tools for modelling average curves of homogeneous groups of animals, with the main aim of predicting yields for management purposes. The increased availability of records per individual lactations and the genetic evaluation based on test day records has shifted the interest of modellers towards more flexible and general linear functions, as polynomials or splines. Thus the main interest of modelling is no longer the reconstruction of the general pattern of the phenomenon but the fitting of individual deviations from an average curve. Other specific approaches based on the modelling of the correlation structure of test day records within lactation, such as mixed linear models or principal component analysis, have been used to test the statistical significance of fixed effects in dairy experiments or to create new variables expressing main lactation curve traits. The adequacy of a model is not an absolute requisite, because it has to be assessed according to the specific purpose it is used for. Occurrence of extended lactations and of new productive and functional traits to be described and the increase of records coming from automatic milking systems likely will represent some of the future challenges for the mathematical modelling of the lactation curve in dairy cattle

Available Pathways for Operationalizing Circular Economy into the Olive Oil Supply Chain: Mapping Evidence from a Scoping Literature Review

Circular economy (CE) is increasingly seen as a promising paradigm for transitioning agri-food systems towards more sustainable models of production and consumption, enabling virtuous and regenerative biological metabolisms based on strategies of eco-efficiency and eco-effectiveness. This contribution seeks to provide a theoretical and empirical framework for operationalizing the CE principles into the olive oil supply chain, that plays a central role in the agroecological systems of the Mediterranean region. A scoping literature review has been conducted in order to identify the available pathways so far explored by scholars for reshaping the olive oil supply chain from a circular perspective. The analyzed literature has been charted on the base of the circular pathway examined, and according to the supply chain subsystem(s) to which it refers. Results are discussed highlighting the main issues, the technology readiness level of the available pathways, the prevailing approaches and knowledge gaps. A synthetic evidence map is provided, framing visually the scrutinized pathways into the Ellen MacArthur Foundation’s CE ‘butterfly’ graph. The work is intended to be a valuable baseline for inquiring how circularity can be advanced in the specific supply chain of olive oil, and which are the strategic opportunities, as well as the barriers to overcome, in order to foster the transition

Exosomes as Intercellular Signaling Organelles Involved in Health and Disease: Basic Science and Clinical Applications

Cell to cell communication is essential for the coordination and proper organization of different cell types in multicellular systems. Cells exchange information through a multitude of mechanisms such as secreted growth factors and chemokines, small molecules (peptides, ions, bioactive lipids and nucleotides), cell-cell contact and the secretion of extracellular matrix components. Over the last few years, however, a considerable amount of experimental evidence has demonstrated the occurrence of a sophisticated method of cell communication based on the release of specialized membranous nano-sized vesicles termed exosomes. Exosome biogenesis involves the endosomal compartment, the multivesicular bodies (MVB), which contain internal vesicles packed with an extraordinary set of molecules including enzymes, cytokines, nucleic acids and different bioactive compounds. In response to stimuli, MVB fuse with the plasma membrane and vesicles are released in the extracellular space where they can interact with neighboring cells and directly induce a signaling pathway or affect the cellular phenotype through the transfer of new receptors or even genetic material. This review will focus on exosomes as intercellular signaling organelles involved in a number of physiological as well as pathological processes and their potential use in clinical diagnostics and therapeutics

Large-scale wave breaking over a barred beach : SPH numerical simulation and comparison with experiments

Acknowledgements The experiments were supported by the European Community’s Horizon 2020 Programme through the grant to the budget of the integrated infrastructure initiative HYDRALAB＋, Contract no. 654110, and were conducted as part of the transnational access project HYBRID. Dr. Corrado Altomare acknowledges funding from the Spanish government and the European Social Found (ESF) under the programme ’Ramón y Cajal 2020’ (RYC2020-030197- /AEI/10.13039/501100011033). Pietro Scandura acknowledges the support received from the University of Catania, Italy by funding the research project ‘Valutazione del rischio idraulico in sistemi complessi (VARIO)’.Peer reviewedPublisher PD

Personalized Treatment of Vulvar Cancer

Vulvar cancer (VC) accounts for 5% of all gynecologic cancer and the most common histological type is squamous cell carcinoma (up to 90%

Mixed Reality for Orthopedic Elbow Surgery Training and Operating Room Applications: A Preliminary Analysis

The use of Mixed Reality in medicine is widely documented to be a candidate to revolutionize surgical interventions. In this paper we present a system to simulate k-wire placement, that is a common orthopedic procedure used to stabilize fractures, dislocations, and other traumatic injuries. With the described system, it is possible to leverage Mixed Reality (MR) and advanced visualization techniques applied on a surgical simulation phantom to enhance surgical training and critical orthopedic surgical procedures. This analysis is centered on evaluating the precision and proficiency of k-wire placement in an elbow surgical phantom, designed with a 3D modeling software starting from a virtual 3D anatomical reference. By visually superimposing 3D reconstructions of internal structures and the target K-wire positioning on the physical model, it is expected not only to improve the learning curve but also to establish a foundation for potential real-time surgical guidance in challenging clinical scenarios. The performance is measured as the difference between K-wires real placement in respect to target position; the quantitative measurements are then used to compare the risk of iatrogenic injury to nerves and vascular structures of MR- guided vs non MR-guided simulated interventions