Evaluating the ≤10:1 wholegrain criterion in identifying nutrient quality and health implications of UK breads and breakfast cereals

This article has been published in a revised form in Public Health Nutrition DOI: https://doi.org/10.1017/S1368980017003718. This version is free to view and download for private research and study only. Not for re-distribution, re-sale or use in derivative works. © 2017 The Authors. Under embargo until 26 June 2018.Objective: To evaluate the nutrient quality of breads and breakfast cereals identified using the wholegrain definition of ≤10:1 carbohydrate:fibre ratio. Design: Following a cross-sectional study design, nutritional information was systematically gathered from food labels of breads and breakfast cereals that met the ≤10:1 carbohydrate:fibre criterion. The median nutrient content was compared with the UK Food Standards Agency nutrient profiling standards and the association between carbohydrate:fibre ratio and other nutrients were analysed. Subgroup analyses were undertaken for products with and without fruit, nuts and/or seeds. Setting: Products from four major supermarket stores in the UK. Subjects: 162 breads and 266 breakfast cereals. Results: Breads which met the ≤10:1 criterion typically contained medium fat, low saturated fat, low sugar and medium sodium. Breakfast cereals typically contained medium fat, low saturated fat, high sugar and low sodium. In both groups, as the carbohydrate:fibre ratio decreased, fat content increased (bread: p=0.029, r=-0.171; breakfast cereal: p=0.033, r=-0.131) and, in breakfast cereals, as the ratio increased, sugar content increased (p<0.0005, r=0.381). Breakfast cereals with fruit, nuts and/or seeds contained, per 100 g, more energy (p=0.002), fat, saturated fat and sugar (all p<0.0005) while seeded breads had more energy, fat and saturated fat (all p<0.0005). Conclusions: Overall, breads and breakfast cereals meeting the ≤10:1 criterion have good nutritional quality, suggesting that the criterion could be useful in public health and/or food labelling. The utility of applying the 10:1 criterion to products containing fruit, nuts and/or seeds is less clear and requires further research.Peer reviewedFinal Accepted Versio

Local scour due to free fall jets in non-uniform sediment

AbstractThe results of experiments on the local scour due to free fall jets are presented in this paper. Experiments were conducted for various values of the densimetric Froude number, the relative tailwater depth, the relative drop height and the relative sediment size. It has been found that by increasing the sediment non-uniformity parameter the scour hole parameters decrease. Moreover, in non-uniform sediment, d90 can be used instead of d50 in the densimetric Froude number of the jet. By using the present and previous experimental data, new equations for the scour parameters were developed. The validity of the developed equations was checked by available prototype data on the scour depth

Experimental Investigation of Leakage Flow Rate and Fluidisation beneath Polyethylene Pipes in Non-Uniform Soils

This is the final version. Available on open access from MDPI via the DOI in this recordData Availability Statement: Some or all of the data, models, or code that support the findings of this study are available from the corresponding author upon reasonable request.Soil fluidisation around buried pipes is one of the water leakage effects that has a direct influence on the ultimate failure of pipelines. In this research, using a laboratory model, the fluidisation caused by water leakage from three cracks with three lengths (14, 17, and 20 mm) and a 3 mm diameter hole for five pressures (1.5–5.5 bar) in non-uniform soils has been evaluated. The experiments were carried out both for pipes buried in soil, as well as exposed pipes. In the buried pipe tests, leakage flow rate, fluidisation, and mobile bed zone dimensions were investigated. The results showed that the increase in leakage flow rate due to an increase in pressure and crack length in exposed pipes is higher than in buried pipes. The exponent of the leakage–pressure relationship was obtained between 0.40 and 0.47 for the hole and between 0.8 and 1.9 in the crack. Observing different development patterns for fluidisation and mobile bed zones in cracks and holes, new relationships are presented for the height, width, and cross-sectional area of the leakage zones

Association of TNF-α G308A gene polymorphism in essential hypertensive patients without type 2 diabetes mellitus

This study aims to investigate the effects of tumor necrosis factor alpha (TNF-α) G308A gene polymorphism on essential hypertension (EHT) with or without type 2 diabetes mellitus (T2DM). The project was conducted on buccal epithelial and blood cells for case and control patients, respectively. Epithelial cells were obtained from the inner part of the cheeks. Techniques including DNA extraction, polymerase chain reaction (PCR), and restriction fragment length polymorphism (RFLP) were utilized to assess biomarkers of DNA damage. Our results demonstrated significant differences between wild and mutated genotypes among EHT patients without T2DM. We also found a significant association between wild and mutated allele frequencies in EHT patients (P < 0.05). Clinical characteristics between the groups (EHT with or without T2DM and controls) showed statistically significant association (P < 0.05). Overall, we show that G308A polymorphism of the TNF-αgene may be a significant genetic risk factor for EHT without T2DM patients in Malaysia

Spectrum of Genetic Changes in Patients with Non-Syndromic Hearing Impairment and Extremely High Carrier Frequency of 35delG GJB2 Mutation in Belarus

The genetic nature of sensorineural hearing loss (SNHL) has so far been studied for many ethnic groups in various parts of the world. The single-nucleotide guanine deletion (35delG) of the GJB2 gene coding for connexin 26 was shown to be the main genetic cause of autosomal recessive deafness among Europeans. Here we present the results of the first study of GJB2 and three mitochondrial mutations among two groups of Belarusian inhabitants: native people with normal hearing (757 persons) and 391 young patients with non-syndromic SNHL. We have found an extremely high carrier frequency of 35delG GJB2 mutation in Belarus −5.7%. This point deletion has also been detected in 53% of the patients with SNHL. The 312del14 GJB2 was the second most common mutation in the Belarus patient cohort. Mitochondrial A1555G mt-RNR1 substitution was found in two SNHL patients (0.55%) but none were found in the population cohort. No individuals carried the A7445G mutation of mitochondrial mt-TS1. G7444A as well as T961G substitutions were detected in mitochondrial mt-RNR1 at a rate of about 1% both in the patient and population cohorts. A possible reason for Belarusians having the highest mutation carrier frequency in Europe 35delG is discussed

Genetic and environmental mechanisms underlying stability and change in problem behaviors at ages 3, 7, 10, and 12.

Maternal ratings on internalizing (INT) and externalizing (EXT) behaviors were collected in a large, population-based longitudinal sample. The numbers of participating twin pairs at ages 3, 7, 10, and 12 were 5,602, 5,115, 2,956, and 1,481, respectively. Stability in both behaviors was accounted for by genetic and shared environmental influences. The genetic contribution to stability (INT: 43%; EXT: 60%) resulted from the fact that a subset of genes expressed at an earlier age was still active at the next time point. A common set of shared environmental factors operated at all ages (INT: 47%; EXT: 34%). The modest contribution of nonshared environmental factors (INT: 10%; EXT: 6%) could not be captured by a simple model. Significant age-specific influences were found for all components, indicating that genetic and environmental factors also contributed to changes in problem behavior. Understanding the origins, nature, and course of psychopathology across childhood is important for clinical purposes as well as for scientific purposes. Of particular clinical importance are the mechanisms underlying continuity. The longer an individual continues along a maladaptive pathway, the more difficult it is to reclaim a normal developmental trajectory (Sroufe, 1990). Further

MRPS18CP2 alleles and DEFA3 absence as putative chromosome 8p23.1 modifiers of hearing loss due to mtDNA mutation A1555G in the 12S rRNA gene

<p>Abstract</p> <p>Background</p> <p>Mitochondrial DNA (mtDNA) mutations account for at least 5% of cases of postlingual, nonsyndromic hearing impairment. Among them, mutation A1555G is frequently found associated with aminoglycoside-induced and/or nonsyndromic hearing loss in families presenting with extremely variable clinical phenotypes. Biochemical and genetic data have suggested that nuclear background is the main factor involved in modulating the phenotypic expression of mutation A1555G. However, although a major nuclear modifying locus was located on chromosome 8p23.1 and regardless intensive screening of the region, the gene involved has not been identified.</p> <p>Methods</p> <p>With the aim to gain insights into the factors that determine the phenotypic expression of A1555G mutation, we have analysed in detail different genetic and genomic elements on 8p23.1 region (<it>DEFA3 </it>gene absence, <it>CLDN23 </it>gene and <it>MRPS18CP2 </it>pseudogene) in a group of 213 A1555G carriers.</p> <p>Results</p> <p>Family based association studies identified a positive association for a polymorphism on <it>MRPS18CP2 </it>and an overrepresentation of <it>DEFA3 </it>gene absence in the deaf group of A1555G carriers.</p> <p>Conclusion</p> <p>Although none of the factors analysed seem to have a major contribution to the phenotype, our findings provide further evidences of the involvement of 8p23.1 region as a modifying locus for A1555G 12S rRNA gene mutation.</p

Towards Alignment Independent Quantitative Assessment of Homology Detection

Identification of homologous proteins provides a basis for protein annotation. Sequence alignment tools reliably identify homologs sharing high sequence similarity. However, identification of homologs that share low sequence similarity remains a challenge. Lowering the cutoff value could enable the identification of diverged homologs, but also introduces numerous false hits. Methods are being continuously developed to minimize this problem. Estimation of the fraction of homologs in a set of protein alignments can help in the assessment and development of such methods, and provides the users with intuitive quantitative assessment of protein alignment results. Herein, we present a computational approach that estimates the amount of homologs in a set of protein pairs. The method requires a prevalent and detectable protein feature that is conserved between homologs. By analyzing the feature prevalence in a set of pairwise protein alignments, the method can estimate the number of homolog pairs in the set independently of the alignments' quality. Using the HomoloGene database as a standard of truth, we implemented this approach in a proteome-wide analysis. The results revealed that this approach, which is independent of the alignments themselves, works well for estimating the number of homologous proteins in a wide range of homology values. In summary, the presented method can accompany homology searches and method development, provides validation to search results, and allows tuning of tools and methods

Repeated exposure to socioeconomic disadvantage and health selection as life course pathways to mid-life depressive and anxiety disorders

The biomedical examination was funded by Medical Research Council [G0000934], awarded under the Health of the Public initiative. Charlotte Clark is supported by an Engineering and Physical Sciences Research Fellowship. Bryan Rodgers is supported by Research Fellowships Nos 148948 and 366758 and by Program Grant No. 179805 from the National Health and Medical Research Council of Australia. Research at the Institute of Child Health and Great Ormond Street Hospital for Children NHS Trust benefits from R&D funding received from the NHS Executive