Antiproliferative Effects Of The Orthosiphon Stamineus And Tualang Honey On Oral Squamous Cell Carcinoma And Human Osteosarcoma Cell Lines

Cancer is the fourth leading cause of death in Malaysia with almost 30,000 people contracting the disease annually. One of the important research fields is the beneficial effects of plants and natural products as effective chemotherapeutic agents. Honey exhibits a broad spectrum of therapeutic properties including antibacterial, antifungal, anti-inflammatory and moderate anticancer effects. It contains many biologically active compounds such as caffeic acid, caffeic acid phenethyl ester and flavonoid glycones which have been proven to exhibit inhibitory effect on tumor cell proliferation. Orthosiphon stamineus (O. stamineus) or ‘misai kucing’ is a popular traditional folk medicine used as medicinal herb for many centuries. It has many medicinal properties such as antioxidant activity, antifungal activity, diuretic activity, antidiabetic activity, anti-inflammatory activity. No previous study has been done to evaluate the effect of Tualang honey and O. stamineus on oral squamous cell carcinoma (OSCC) and human osteosarcoma (HOS). This study was aimed to verify the antiproliferative activity for several concentrations of Tualang honey, O. stamineus and mixed concentrations of honey and O. stamineus on OSCC and HOS cell lines. Several doses of Tualang honey (1% - 20%), several concentrations of O. stamineus (100μg/ml to 1mg/ml) and several mixed solutions of both were applied on OSCC and HOS cell culture for 3, 6, 12, 24, 48 and 72 hours

A web for Supreme Judicial Institute Library in Yemen

This chapter discusses about the development of a website for Supreme Judicial Institute Library in Yemen. This project intended to serve (S1I)Library administration, staff (lecturers, researchers, and management), and students. The objectives of this project is to develop and build a website for the SJI library in Yemen to provide Automated searching, booking, and checking the statues of the book by remote users anywhere and anytime,Automated book management to add, update, save, borrow and delete books and journals and their information, and Automated users and staff management to add, update, save and delete their information accordingly. The system is developed using Macromedia Dreamweaver, PHP, and MySQL

Antiproliferative effect of Tualang honey on oral squamous cell carcinoma and osteosarcoma cell lines

<p>Abstract</p> <p>Background</p> <p>The treatment of oral squamous cell carcinomas (OSCC) and human osteosarcoma (HOS) includes surgery and/or radiotherapy which often lead to reduced quality of life. This study was aimed to study the antiproliferative activity of local honey (Tualang) on OSCC and HOS cell lines.</p> <p>Methods</p> <p>Several concentrations of Tualang honey (1% - 20%) were applied on OSCC and HOS cell lines for 3, 6, 12, 24, 48 and 72 hours. Morphological characteristics were observed under light and fluorescent microscope. Cell viability was assessed using MTT assay and the optical density for absorbance values in each experiment was measured at 570 nm by an ELISA reader. Detection of cellular apoptosis was done using the Annexin V-FITC Apoptosis Detection Kit.</p> <p>Results</p> <p>Morphological appearance showed apoptotic cellular changes like becoming rounded, reduction in cell number, blebbed membrane and apoptotic nuclear changes like nuclear shrinkage, chromatin condensation and fragmented nucleus on OSCC and HOS cell lines. Cell viability assay showed a time and dose-dependent inhibitory effect of honey on both cell lines. The 50% inhibitory concentration (IC_<b>50</b>) for OSCC and HOS cell lines was found to be 4% and 3.5% respectively. The maximum inhibition of cell growth of ≥80% was obtained at 15% for both cell lines. Early apoptosis was evident by flow cytometry where percentage of early apoptotic cells increased in dose and time dependent manner.</p> <p>Conclusion</p> <p>Tualang honey showed antiproliferative effect on OSCC and HOS cell lines by inducing early apoptosis.</p

Prevalence of Oral Mucosal Lesions in Patients with Type 2 Diabetes Attending Hospital Universiti Sains Malaysia

Objectives: Diabetes mellitus is associated with a greater likelihood of developing certain oral mucosal disorders. This study was aimed at assessing the prevalence of oral mucosal lesions (OMLs) in patients with type 2 diabetes (DM2) and to investigate the association of such lesions with metabolic control of the disease. Methods: This cross-sectional study involved 391 patients with DM2 and 391 non-diabetic control subjects. Demographic information and data on the duration and type of diabetes, glycosylated hemoglobin (HbA1c) values, medical history, and current use of medication were obtained from medical records. Detailed oral examination was performed in accordance with international criteria. Results: The prevalence of OMLs was significantly higher among diabetic patients (45.5%) than among control subjects (38.4%) (P = 0.042). Patients with diabetes had a higher prevalence of geographic tongue (GT) (P = 0.017), denture stomatitis (P = 0.018), and angular cheilitis (P = 0.006) than controls. Overall, diabetic patients with poor metabolic control had a significantly higher prevalence of OMLs and xerostomia than patients with moderately and well-controlled disease (P < 0.05). Conclusions: The prevalence of OMLs was significantly higher in diabetic patients than in control subjects. Higher occurrence of OMLs was significantly associated with poor metabolic control

Effect of Erica sp. Honey against Microorganisms of Clinical Importance: Study of the Factors Underlying this Biological Activity

This study aimed to determine the factors (phenolic compounds, flavonoids, sugars or H2O2) that contribute the most to the antimicrobial activity of heather honey samples against four yeasts and four bacteria with medical importance. To discard the effect of H2O2 in the antimicrobial activity, catalase was added. To evaluate the osmotic pressure’s effect, artificial honey was also used. Phenolic compounds and flavonoids were determined and Pearson’s correlation analysis was performed to assess whether these correlated with antimicrobial activity. The amount of phenolic compounds ranged from 630.89 ± 5.21 GAE kg−1 to 718.92 ± 4.41 GAE kg−1, while the flavonoids varied between 450.72 ± 5.67 CAE kg−1 and 673.98 ± 4.33 CAE kg−1. For the bacteria, the minimum inhibitory concentration (MIC) of the honey without catalase ranged from 1.01 ± 0.50% to 10.00 ± 4.72% and was between 2.00 ± 0.94% and 13.27 ± 5.23% for honey with catalase. Concerning the yeasts, the MICs was between 13.16 ± 4.08% and 20.00 ± 5.09% for honey without catalase and between 14.95 ± 4.16% and 25.67 ± 5.50% for honey with catalase. The elucidation of the antimicrobial factors and action mechanisms is essential for the correct use of honey in therapeutic applications