Determination of Hydrazlne Using Potassium Dichromate as Oxidizing Agent

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Simultaneous genotyping and species identification using hybridization pattern recognition analysis of generic Mycobacterium DNA arrays

High-density oligonucleotide arrays can be used to rapidly examine large amounts of DNA sequence in a high throughput manner. An array designed to determine the specific nucleotide sequence of 705 bp of the rpoB gene of Mycobacterium tuberculosis accurately detected rifampin resistance associated with mutations of 44 clinical isolates of M. tuberculosis. The nucleotide sequence diversity in 121 Mycobacterial isolates (comprised of 10 species) was examined by both conventional dideoxynucleotide sequencing of the rpoB and 165 genes and by analysis of the rpoB oligonucleotide array hybridization patterns. Species identification for each of the isolates was similar irrespective of whether 16S sequence, rpoB sequence, or the pattern of rpoB hybridization was used. However, for several species, the number of alleles in the 16S and rpoB gene sequences provided discordant estimates of the genetic diversity within a species. In addition to confirming the array's intended utility for sequencing the region of M. tuberculosis that confers rifampin resistance, this work demonstrates that this array can identify the species of nontuberculous Mycobacteria. This demonstrates the general point that DNA microarrays that sequence important genomic regions (such as drug resistance or pathogenicity islands) can simultaneously identify species and provide some insight into the organism's population structure

Hamiltonian quantization of General Relativity with the change of signature

We show in this article how the usual hamiltonian formalism of General Relativity should be modified in order to allow the inclusion of the Euclidean classical solutions of Einstein's equations. We study the effect that the dynamical change of signature has on the superspace and we prove that it induces a passage of the signature of the supermetric from ($-+++++$) to ($+-----$). Next, all these features are more particularly studied on the example of minisuperspaces. Finally, we consider the problem of quantization of the Euclidean solutions. The consequences of different choices of boundary conditions are examined.Comment: 32 pages, GCR-93/11/01, To appear in Phys. Rev.

Implications of invariance of the Hamiltonian under canonical transformations in phase space

We observe that, within the effective generating function formalism for the implementation of canonical transformations within wave mechanics, non-trivial canonical transformations which leave invariant the form of the Hamilton function of the classical analogue of a quantum system manifest themselves in an integral equation for its stationary state eigenfunctions. We restrict ourselves to that subclass of these dynamical symmetries for which the corresponding effective generating functions are necessaarily free of quantum corrections. We demonstrate that infinite families of such transformations exist for a variety of familiar conservative systems of one degree of freedom. We show how the geometry of the canonical transformations and the symmetry of the effective generating function can be exploited to pin down the precise form of the integral equations for stationary state eigenfunctions. We recover several integral equations found in the literature on standard special functions of mathematical physics. We end with a brief discussion (relevant to string theory) of the generalization to scalar field theories in 1+1 dimensions.Comment: REVTeX v3.1, 13 page

Infinite Symmetry in the Quantum Hall Effect

Free planar electrons in a uniform magnetic field are shown to possess the symmetry of area-preserving diffeomorphisms ($W$-infinity algebra). Intuitively, this is a consequence of gauge invariance, which forces dynamics to depend only on the flux. The infinity of generators of this symmetry act within each Landau level, which is infinite-dimensional in the thermodynamical limit. The incompressible ground states corresponding to completely filled Landau levels (integer quantum Hall effect) are shown to be infinitely symmetric, since they are annihilated by an infinite subset of generators. This geometrical characterization of incompressibility also holds for fractional fillings of the lowest level (simplest fractional Hall effect) in the presence of Haldane's effective two-body interactions. Although these modify the symmetry algebra, the corresponding incompressible ground states proposed by Laughlin are again symmetric with respect to the modified infinite algebra.Comment: 28 page

Tibial tubercle osteotomy for access during revision knee arthroplasty: Ethibond suture repair technique

<p>Abstract</p> <p>Background</p> <p>Tibial Tubercle Osteotomy has shown much promise in revision total knee replacement. Methods of repair previously described include screw and wire fixation. Both methods have significant complications.</p> <p>Methods</p> <p>This article describes suture fixation of the osteotomy using Ethibond sutures placed medially with a lateral periosteal hinge.</p> <p>Results</p> <p>This method of fixation relies upon an adequate osteotomy segment including the entire insertion of the patella tendon. The lateral periosteal hinge is maintained and adds to the stability of the construct. A minimum of two number 5 Ethibond sutures are passed medially through drill holes to secure the osteotomy segment. No post-operative immobilisation is required.</p> <p>Conclusion</p> <p>Ethibond sutures provide adequate fixation of the tibial tubercle osteotomy segment in revision knee arthroplasty with reduced risk of complication as compared to conventional fixation methods.</p

Quantum equivalence of sigma models related by non Abelian Duality Transformations

Coupling constant renormalization is investigated in 2 dimensional sigma models related by non Abelian duality transformations. In this respect it is shown that in the one loop order of perturbation theory the duals of a one parameter family of models, interpolating between the SU(2) principal model and the O(3) sigma model, exhibit the same behaviour as the original models. For the O(3) model also the two loop equivalence is investigated, and is found to be broken just like in the already known example of the principal model.Comment: As a result of the collaboration of new authors the previously overlooked gauge contribution is inserted into eq.(43) changing not so much the formulae as part of the conclusion: for the models considered non Abelian duality is OK in one loo

Gluten sensitivity enteropathy in patients with recurrent aphthous stomatitis

<p>Abstract</p> <p>Background</p> <p>Gluten sensitive enteropathy (GSE) is an autoimmune enteropathy triggered by the ingestion of gluten-containing grains in susceptible individuals. Recurrent aphthous stomatitis (RAS) may be the sole manifestation of GSE. The aim of this study was to determine the prevalence of gluten sensitivity enteropathy (GSE) in a large group of patients with RAS and assess the efficacy of gluten free diet (GFD) on the improvement of aphthous lesions in those who were diagnosed with GSE.</p> <p>Methods</p> <p>Two hundred and forty seven patients with RAS were included. The patients had at least three aphthous attacks per year. Patients were screened by IgA anti-endomysial antibody (EMA), IgA anti tissue transglutaminase (TTG) and serum IgA level. Those with a positive serology underwent endoscopic biopsies of the duodenal mucosa and patients with negative serology were excluded. The diagnosis of GSE was based on a positive serological test and abnormal duodenal histology. For patients with GSE, gluten free diet was recommended.</p> <p>Results</p> <p>Six out of 247 RAS patients had positive TTG test alone, and one had positive EMA and TTG. All 7 patients with positive serologic tests underwent duodenal biopsies. Histological findings were compatible with GSE in all of them (Marsh I in four patients, Marsh II in two patients and Marsh IIIB in one another.). The mean age of GSE patients was 27.42 ± 10.56 (range, 13 to 40) years old. They were suffering from RAS for an average duration of 4.5 years. All of the 7 GSE patients had not responded to the routine anti-aphthae medications, including topical corticosteroids, tetracycline and colchicine. Four patients who adhered to a strict gluten-free diet showed noticeable improvement in their aphthous lesions over a period of 6 months.</p> <p>Conclusion</p> <p>A significant minority (e.g. 2.83%) of RAS patients have GSE. This could be compared with the 0.9% prevalence of GSE in the general population of Iran. This study suggests that evaluation for celiac disease is appropriate in patients with RAS. Additionally, the unresponsiveness to conventional anti-aphthae treatment could be an additional risk indicator.</p