CARACTERÍSTICAS DEL QUESO TEPEQUE DE LA TIERRA CALIENTE DE MICHOACÁN: UN QUESO PRODUCIDO EN UN SISTEMA SILVOPASTORIL INTENSIVO

El objetivo del presente estudio fue describir las características del Queso Tepeque de la Tierra Caliente del Estado de Michoacán en términos de su región de origen, proceso de manufactura, características fisicoquímicas y microbiológicas, y documentar los cambios atribuibles a la implementación de un sistema silvopastoril intensivo (SSPi) para la alimentación del ganado. La metodología consintió en visitar la zona de estudio para recolectar información sobre el territorio donde se produce el queso, se realizaron entrevistas semi- estructuradas a productores para conocer el sistema de producción de leche y el proceso de elaboración del queso. Se determinaron las características físicas (pH y densidad) y químicas (contenidos de proteína, grasa, lactosa, sólidos no grasos y agua adicionada) de la leche que se emplea en la elaboración del queso. En el queso se determinó su contenido de proteína, grasa, sal, humedad, cenizas y pH. Se determinaron las características sanitarias de ambos productos incluyendo conteo de microorganismos mesófilos aerobios, coliformes, hongos, levaduras y la presencia de Listeria spp y Staphylococcus aureus. El estudio se llevó a cabo en el año 2010, se dividió en dos períodos, época de lluvias y época de estiaje. Se estudiaron 15 productores, siete del SSPi y ocho del sistema tradicional de producción de leche. Se observó que el Queso Tepeque es un producto de excelente calidad desde el punto de vista de sus principales nutrientes, está elaborado con leche de buena calidad fisicoquímica, tiene una historia y tradición que supera los 300 años. El queso elaborado con leche de SSPi presenta características similares (P>0.05) e incluso superiores en el contenido de grasa (P0.05) atribuibles al SSPi en ninguno de los parámetros evaluados. Finalmente, la calidad sanitaria de la leche y el queso fue deficiente en ambos sistemas, aunque el proceso de maduración atenúa este problema, por lo que el consumo de queso madurado y añejado se recomienda en lugar del queso oreado

Effect of different growth stages of rapeseed (brassica rapa L.) on nutrient intake and digestibility, nitrogen balance, and rumen fermentation kinetics in sheep diets

The objective of the present study was to determine the dietary effect of different growth stages of rapeseed (Brassica rapa L.) on nutrient intake and digestibility, nitrogen balance, and rumen fermentation kinetics in sheep.The objective of the present study was to determine the dietary effect of different growth stages of rapeseed (Brassica rapa L.) on nutrient intake and digestibility, nitrogen balance, and rumen fermentation kinetics in sheep. Four dietary treatments were utilised. A basal control diet based on alfalfa hay, oat hay, soybean meal and corn grain. Then alfalfa hay was replaced with 300 g/kg DM of rapeseed forage harvested at three different growth stages: Vegetative, Flowering and Pod. In vitro gas production was determined using three rumen cannulated Suffolk sheep in a completely randomised design, and nutrients intake and digestibility of each diet were determined using four Suffolk sheep in a 4 4 Latin square design with 21 d periods consisting of 14 d for diet adaptation and 7 d for sample collection. Feed intake and excretion of faeces and urine were recorded. Dry matter intake was higher for control and Pod compared to Vegetative and Flowering. The digestibility of dry matter, organic matter, neutral detergent fibre and acid detergent fibre were similar among treatments. Nitrogen intake was higher for control and Pod and lower for Vegetative and Flowering. In vitro gas production was similar among treatments (P > .05). In vitro gas yield at 24 h was higher (P < .05) for control than the rest of the treatments. Overall, inclusion of 300 g/kg DM of rapeseed forage harvested at pod stage as a substitute for alfalfa hay is an alternative source of protein without affecting nutrient intake and digestibility.Amelia Zetina Sanchez thanked the National Council for Science and Technology (Conacyt, Mexico) for the scholarship for her studies at the Universidad Nacional Autonoma de Mexico. Lizbeth Esmeralda Robles Jimenez was awarded a Conacyt-Mexico scholarship during her PhD program at the Agricultural Sciences and Natural Resources at the Universidad Autonoma del Estado de México. During the study, Dr. Einar Vargas-Bello-Pérez was a visiting scholar also supported by project number 4974/2020CIB from Universidad Autonoma del Estado de México

Impact of COVID-19 on cardiovascular testing in the United States versus the rest of the world

Objectives: This study sought to quantify and compare the decline in volumes of cardiovascular procedures between the United States and non-US institutions during the early phase of the coronavirus disease-2019 (COVID-19) pandemic. Background: The COVID-19 pandemic has disrupted the care of many non-COVID-19 illnesses. Reductions in diagnostic cardiovascular testing around the world have led to concerns over the implications of reduced testing for cardiovascular disease (CVD) morbidity and mortality. Methods: Data were submitted to the INCAPS-COVID (International Atomic Energy Agency Non-Invasive Cardiology Protocols Study of COVID-19), a multinational registry comprising 909 institutions in 108 countries (including 155 facilities in 40 U.S. states), assessing the impact of the COVID-19 pandemic on volumes of diagnostic cardiovascular procedures. Data were obtained for April 2020 and compared with volumes of baseline procedures from March 2019. We compared laboratory characteristics, practices, and procedure volumes between U.S. and non-U.S. facilities and between U.S. geographic regions and identified factors associated with volume reduction in the United States. Results: Reductions in the volumes of procedures in the United States were similar to those in non-U.S. facilities (68% vs. 63%, respectively; p = 0.237), although U.S. facilities reported greater reductions in invasive coronary angiography (69% vs. 53%, respectively; p < 0.001). Significantly more U.S. facilities reported increased use of telehealth and patient screening measures than non-U.S. facilities, such as temperature checks, symptom screenings, and COVID-19 testing. Reductions in volumes of procedures differed between U.S. regions, with larger declines observed in the Northeast (76%) and Midwest (74%) than in the South (62%) and West (44%). Prevalence of COVID-19, staff redeployments, outpatient centers, and urban centers were associated with greater reductions in volume in U.S. facilities in a multivariable analysis. Conclusions: We observed marked reductions in U.S. cardiovascular testing in the early phase of the pandemic and significant variability between U.S. regions. The association between reductions of volumes and COVID-19 prevalence in the United States highlighted the need for proactive efforts to maintain access to cardiovascular testing in areas most affected by outbreaks of COVID-19 infection

Estudios de Caso sobre Ciencias Agropecuarias y Rurales en el siglo XXI.

Libro científico sobre estudios de casos en el medio agropecuario y ruralCon el advenimiento del siglo XXI y el avance de los procesos de globalización, el medio rural presenta diversos cambios económicos, sociales, políticos y culturales. Lo anterior significa que el campo es un objeto de estudio altamente dinámico, complejo e inasible. las ciencias agropecuarias y rurales, en la actualidad, requieren de un abordaje sistémico e interdisciplinario que den cuenta de la heterogeneidad de situaciones y contextos que enfrenta el campo mexicano. La presente obra agrupa 18 estudios de caso, que capturan algunas fotografías de las diversas problemáticas de la ruralidad mexicana, con lo cual se pretende dar cuenta tanto de los objetivos de estudio como de la perspectiva teórico metodológico desde que estos son abordados. lo anterior tiene que ver con el hecho de que las ciencias agropecuarias y rurales manifiestan un alto grado de observación empírica, motivo por el que los estudios de caso se convierten en la perspectiva metodológica idónea que permite ir y venir de la realidad a la teoría y viceversa para la construcción de objetos de estudio. En este volumen se aborda una gran diversidad de casos, que sintetizan la heterogeneidad de enfoques y perspectivas mediante las cuales los fenómenos agropecuarios y rurales han sido abordados en el Instituto de Ciencias Agropecuarias y Rurales de la Universidad Autónoma del Estado de México, en los últimos 30 años

Com o diabo no corpo: os terríveis papagaios do Brasil colônia

Desde a Antiguidade, papagaios, periquitos e afins (Psittacidae) fascinaram os europeus por seu vivo colorido e uma notável capacidade de interação com seres humanos. A descoberta do Novo Mundo nada faria além de acrescentar novos elementos ao tráfico de animais exóticos há muito estabelecido pelos europeus com a África e o Oriente. Sem possuir grandes mamíferos, a América tropical participaria desse comércio com o que tinha de mais atrativo, essencialmente felinos, primatas e aves - em particular os papagaios, os quais eram embarcados em bom número. Contudo, a julgar pelos documentos do Brasil colônia, esses voláteis podiam inspirar muito pouca simpatia, pois nenhum outro animal - exceto as formigas - foi tantas vezes mencionado como praga para a agricultura. Além disso, alguns psitácidas mostravam-se tão loquazes que inspiravam a séria desconfiança de serem animais demoníacos ou possessos, pois só três classes de entidades - anjos, homens e demônios - possuíam o dom da palavra. Nos dias de hoje, vários representantes dos Psittacidae ainda constituem uma ameaça para a agricultura, enquanto os indivíduos muito faladores continuam despertando a suspeita de estarem possuídos pelo demônio. Transcendendo a mera curiosidade, essa crença exemplifica o quão intrincadas podem ser as relações do homem com o chamado “mundo natural”, revelando um universo mais amplo e multifacetado do que se poderia supor a princípio. Nesse sentido, a existência de aves capazes de falar torna essa relação ainda mais complexa e evidencia que as dificuldades de estabelecer o limite entre o animal e o humano se estendem além dos primatas e envolvem as mais inusitadas espécies zoológicas.Since ancient times, parrots and their allies (Psittacidae) have fascinated Europeans by their striking colors and notable ability to interact with human beings. The discovery of the New World added new species to the international exotic animal trade, which for many centuries had brought beasts to Europe from Africa and the Orient. Lacking large mammals, tropical America participated in this trade with its most appealing species, essentially felines, primates and birds - especially parrots - which were shipped in large numbers. It should be noted, however, that at times these birds were not well liked. In fact, according to documents from colonial Brazil, only the ants rank higher than parrots as the animals most often mentioned as agricultural pests. On the other hand, some of these birds were so chatty that people suspected them to be demonic or possessed animals, since only three classes of beings - angels, men and demons - have the ability to speak. Nowadays, several Psittacidae still constitute a threat to agriculture, and the suspicion that extremely talkative birds were demon possessed has also survived. More than a joke or a mere curiosity, this belief exemplifies how intricate man’s relationships with the “natural world” may be. In this sense, the existence of birds that are able to speak adds a further twist to these relationships, demonstrating that the problem of establishing a boundary between the animal and the human does not only involve primates, but also includes some unusual zoological species

Protocol to downregulate GABAergic-astrocyte signaling via astrocyte-selective ablation of GABAB receptor in adult mice

Here, we present a protocol to selectively downregulate GABA receptor (GABAR) expression in astrocytes of mouse medial prefrontal cortex (mPFC). We first describe the procedure of surgeries and viral injections. We then detail genetic, histological, and functional characterizations of astrocytic GABAR ablation using RT-PCR, imaging, and behavioral assays. The use of GABA flox mice can be easily adapted to generate astrocyte-selective GABAR ablation in different brain areas and postnatal stages, leading to local downregulation of GABAergic-astrocyte signaling without developmental issues. For complete details on the use and execution of this protocol, please refer to Mederos et al. (2021).This research was supported by grants from the MICINN/AEI/FEDER, UE-Spain (PRE2020-092999) to A.S.-R.; EMBO ALTF 327-2021 to S.M.; PID2019-106579RB-I00 funded by MCIN/AEI/10.13039/501100011033; ASTROTECH 956325 H2020-MSCA-ITN-2020, and Fundacio ́ La Marato ́TV3 #225619 to G.P

Diagnóstico de la calidad sanitaria en las queserías artesanales del municipio de Zacazonapan, Estado de México

Objetivo. Determinar la prevalencia y el grado de contaminación por mohos y levaduras (M&L), bacterias mesófilas aerobias (BMA), coliformes totales (CT) y Salmonella spp. (S) como indicadores de calidad e higiene en el entorno de fabricación del queso de Zacazonapan. Material y métodos. En cinco queserías se obtuvieron muestras de agua, leche, cuajo y queso, y de superficie de las manos y de los utensilios que están en contacto con el queso. Resultados. Todas las superficies presentaron contaminación; por lo tanto, se observa falta de higiene al elaborar el queso. Los conteos durante la elaboración de queso fueron, para leche, de 6.8, 6.7 y 4.5 log10 UFC/ml para M&L, BMA y CT, respectivamente. En queso, se detectó la presencia de S y cuentas de 9.16, 9.23 y 9.18 log10 UFC/g para M&L, BMA y CT. Conclusiones. La poca higiene en queserías y utensilios al elaborar el queso representa un riesgo para la salud humana

Embryonic Stem Cell-Specific miR302-367 Cluster: Human Gene Structure and Functional Characterization of Its Core Promoter▿ †

MicroRNAs (miRNAs) play a central role in the regulation of multiple biological processes including the maintenance of stem cell self-renewal and pluripotency. Recently, the miRNA cluster miR302-367 was shown to be differentially expressed in embryonic stem cells (ESCs). Unfortunately, very little is known about the genomic structure of miRNA-encoding genes and their transcriptional units. Here, we have characterized the structure of the gene coding for the human miR302-367 cluster. We identify the transcriptional start and functional core promoter region which specifically drives the expression of this miRNA cluster. The promoter activity depends on the ontogeny and hierarchical cellular stage. It is functional during embryonic development, but it is turned off later in development. From a hierarchical standpoint, its activity decays upon differentiation of ESCs, suggesting that its activity is restricted to the ESC compartment and that the ESC-specific expression of the miR302-367 cluster is fully conferred by its core promoter transcriptional activity. Furthermore, algorithmic prediction of transcription factor binding sites and knockdown studies suggest that ESC-associated transcription factors, including Nanog, Oct3/4, Sox2, and Rex1 may be upstream regulators of miR302-367 promoter. This study represents the first identification, characterization, and functional validation of a human miRNA promoter in stem cells. This study opens up new avenues to further investigate the upstream transcriptional regulation of the miR302-367 cluster and to dissect how these miRNAs integrate in the complex molecular network conferring stem cell properties to ESCs